Change of Biomechanical Parameters in the Lower Jaws of Rabbits with Experimental Osteoporosis after Implantation of Calcium-Phosphate Bioceramic Material in the Greater Trochanter Region

2016 ◽  
Vol 721 ◽  
pp. 224-228
Author(s):  
Girts Salms ◽  
Vladislavs Ananjevs ◽  
Vladimirs Kasyanovs ◽  
Andrejs Skagers ◽  
Ilze Salma ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Strontium Ranelate ◽  
Biomechanical Properties ◽  
Control Group ◽  
Greater Trochanter ◽  
Lower Jaw ◽  
Experimental Osteoporosis ◽  
Rabbit Bone ◽  
Biomechanical Parameters

Investigation of biomechanical properties of the rabbit bone tissue from a corner of the lower jaw was done. Experimental osteoporosis was induced by ovariectomy and following injections of methylprednisolone. The defects in the greater trochanter region was created and filled with granules of a hydroxyapatite and tricalcium phosphate (HAP/TCP 30/70) or HAP/TCP 30/70 together with 5% strontium ranelate. After 3 month animals were euthanased, squared samples have been cut out from a corner of the lower jaw and tested on a bend. Results of research show, that the corner of a lower jaw in rabbit becomes more rigid after filling of defects in the greater trochanter region with granules of a hydroxyapatite and tricalcium phosphate (HAP/TCP 30/70) or granules together with strontium ranelate. The ultimate strain for the bone tissue in the 2nd and 3rd group is less, than for control group. Thus, local uses calcium – phosphatic bioceramic material around the greater trochanter region improves biomechanical parameters of a bone tissue in the lower jaw of animals.

scholarly journals Calcium Phosphate Bioceramic Material Local Influence on the Bone Biomechanical Properties at Rabbits with Experimental Osteoporosis

2018 ◽  
Vol 7 (2.23) ◽  
pp. 496
Author(s):  
Vladislavs Ananjevs ◽  
Aleksandra Anaņjeva ◽  
Janis Vetra ◽  
Andrejs Skagers ◽  
Ilze Salma ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Test Group ◽  
Biomechanical Properties ◽  
Local Influence ◽  
Control Groups ◽  
Greater Trochanter ◽  
Experimental Osteoporosis ◽  
Rabbit Cartilage

Biomechanical characteristics of the rabbit cartilage component from the femur were investigated. The control osteoporosis had been activated by ovariectomy alongside the injections of methylprednisolone. During the experiment defects in the femur’s greater trochanter zone was created and afterwards filled with the fragments of a hydroxyapatite as well as tricalcium phosphate (HAP/TCP 70/30) or HAP/TCP 70/30 mixed with five percent strontium. Three months later animals were euthanazed, squared samples were cut out from the femur’s body and later tested on a bend. The results of the research revealed that the femurs become more stringent after filling off defects in the greater trochanter zone with HAP/TCP 70/30 or HAP/TCP 70/30 mixed with five percent strontium. The ultimate pain for the cartilage component in the control groups was less, than in the test group. The flexure modulus of flexibility of a cartilage component in the test group was statistically reliable less, than the value of the flexure modulus of flexibility of the cartilage component in the control groups.  Therefore, local usage of calcium – phosphatic bioceramic component over the greater trochanter part increases biomechanical characteristics of the cartilage component in the femur of animals.  

The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model

2021 ◽  
pp. 194338752110483
Author(s):  
Jonathan Ribeiro da Silva ◽  
Maria Cristina de Moraes Balbas ◽  
Caroline Águeda Corrêa ◽  
Manuella Zanela ◽  
Roberta Okamoto ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Tissue ◽  
Clinical Evaluation ◽  
Tricalcium Phosphate ◽  
Bone Grafts ◽  
Osteonecrosis Of The Jaw ◽  
Control Group ◽  
Bovine Bone ◽  
Beta Tricalcium Phosphate ◽  
Group I

Objective: To evaluate the effects of inorganic bovine bone graft (Lumina Bone, Criteria, Brazil) and beta-tricalcium phosphate (β-TCP) graft (ChronOS, Synthes, Brazil) in rats with the risk of developing post-extraction medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen male Wistar rats weighing 350 to 450 g were induced to develop MRONJ using zoledronic acid for 5 weeks. In the sixth week, the right maxillary first molar was extracted. The animals in Group I (G1) did not receive bone grafts after tooth extraction, while Group II (G2) animals received inorganic bovine bone grafts, and Group III (G3) animals received beta-tricalcium phosphate (β-TCP) grafts. Clinical evaluation and histomorphometric and immunohistochemical analyses were performed. ANOVA and Tukey’s statistical tests were used and a level of significance was considered to be 5%. Results: In the clinical evaluation, animals from G2 and G3 did not present clinical manifestations of osteonecrosis, unlike the control group (G1) animals, which presented necrotic bone tissue exposure in all samples. In the histomorphometric evaluation, animals in G3 showed greater formation of bone tissue (66%) and less formation of bone lacuna (18%) than animals in G1 (58%/32%) and in G2 (59%/27%) ( P < 0.05). Moderate (++) immunostaining was observed in G2 and G3 for RANKL, TRAP, and OC, while G1 showed moderate (++) labeling for OC and mild (+) immunostaining for TRAP and RANKL. Conclusions: Greater formation of bone tissue and fewer bone lacunae were found in animals treated with β-TCP. In clinical evaluation, bone graft groups presented with the clinical manifestation of MRONJ and showed higher intensity of immunostaining for TRAP and RANKL. Despite the limitations of experimental animal studies, the results of this work may assist in the development of future clinical research for the prevention of MRONJ.

scholarly journals Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing

2018 ◽  
Vol 62 (3) ◽  
pp. 385-394 ◽  
Author(s):  
Hadi Eftekhari ◽  
Alireza Jahandideh ◽  
Ahmad Asghari ◽  
Abolfazl Akbarzadeh ◽  
Saeed Hesaraki
Keyword(s):  
Bone Tissue ◽  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Healing Process ◽  
Control Group ◽  
Time Intervals ◽  
Positive Effects ◽  
Bone Scaffolds ◽  
Modern Engineering ◽  
And Control

AbstractIntroductionIn recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications.Material and MethodsThis study was performed on 60 adult male New Zealand rabbits divided into four experimental groups: the control group without any treatment, the second group receiving hydroxyapatite, the third group treated with β-tricalcium phosphate, and the fourth group receiving nanocomposite polycaprolactone (PCL) scaffold. In a surgical procedure, a defect 6 mm in diameter was made in a hind limb femur. Four indexes were used to assess histopathology, which were union index, spongiosa index, cortex index, and bone marrow.ResultsThe results showed that nanocomposite PCL and control groups always had the respective highest and lowest values among all the groups at all time intervals. The histopathological assessment demonstrated that the quantity of newly formed lamellar bone in the nanocomposite PCL group was higher than in other groups.ConclusionAll these data suggest that PCL had positive effects on the bone healing process, which could have great potential in tissue engineering and clinical applications.

scholarly journals Assessment of Corneal Biomechanical Properties and Intraocular Pressure in Myopic Spanish Healthy Population

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
María A. del Buey ◽  
Laura Lavilla ◽  
Francisco J. Ascaso ◽  
Elena Lanchares ◽  
Valentín Huerva ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
High Myopia ◽  
Biomechanical Properties ◽  
Control Group ◽  
Healthy Population ◽  
Iop Measurement ◽  
Group 3 ◽  
Biomechanical Parameters ◽  
Group 2 ◽  
The Relationship

Purpose. To examine biomechanical parameters of the cornea in myopic eyes and their relationship with the degree of myopia in a western healthy population.Methods. Corneal hysteresis (CH), corneal resistance factor (CRF), Goldmann correlated intraocular pressure (IOP), and corneal compensated IOP (IOPcc) were measured using the ocular response analyzer (ORA) in 312 eyes of 177 Spanish subjects aged between 20 and 56 years. Refraction was expressed as spherical equivalent (SE), which ranged from 0 to −16.50 diopters (D) (mean:−3.88±2.90 D). Subjects were divided into four groups according to their refractive status: group 1 or control group: emmetropia (-0.50≤SE<0.50); group 2: low myopia (-0.75≤SE<3.00 D); group 3: moderate myopia (-3.00≤SE≤-6.00 D); and group 3: high myopia (SE greater than −6.00 D). We analyzed the relationship between corneal biomechanics measured with ORA and SE.Results. CH in the emmetropia, low myopia, moderate myopia, and high myopia groups was11.13±0.98,11.49±1.25,10.52±1.54, and10.35±1.33 mmHg, respectively. CH in the highly myopic group was significantly lower than that in the emmetropic group (P=0.07) and low myopic group (P=0.035); however, there were no differences with the moderate myopic group (P=0.872). There were no statistically significant differences regarding IOP among the four groups (P>0.05); nevertheless, IOPcc was significantly higher in the moderately myopic (15.47±2.47 mmHg) and highly myopic (16.14±2.59 mmHg) groups than in the emmetropia (15.15±2.06 mmHg) and low myopia groups (14.53±2.37 mmHg). No correlation between age and the measured parameters was found. CH and IOPcc were weakly but significantly correlated with SE (r=0.171,P=0.002andr=-0.131,P=0.021, resp.).Conclusions. Present study showed only a very weak, but significant, correlation between CH and refractive error, with CH being lower in both moderately and highly myopic eyes than that in the emmetropic and low myopic eyes. These changes in biomechanical properties of the cornea may have an impact on IOP measurement, increasing the risk of glaucoma.

scholarly journals Influence of dextran solution and corneal collagen crosslinking on Corneal Biomechanical Parameters Evaluated by Corvis ST

2021 ◽  
Author(s):  
Xiao Qin ◽  
Lei Tian ◽  
Hui Zhang ◽  
Di Zhang ◽  
Li-Li Guo ◽  
...  
Keyword(s):  
Biomechanical Properties ◽  
Control Group ◽  
Corvis St ◽  
Collagen Crosslinking ◽  
Corneal Collagen Crosslinking ◽  
Biomechanical Parameters ◽  
Dextran Solution ◽  
Corneal Biomechanical Properties ◽  
Porcine Eyes ◽  
Corneal Collagen

Abstract Purpose: To analyze the influence of dextran solution and corneal collagen crosslinking (CXL) on corneal biomechanical parameters evaluated by Corneal Visualization Scheimpflug Technology (Corvis ST). Materials and Methods: Forty porcine eyes were included in this study. Twenty porcine eyes were soaked in dextran solution for 30 minutes (10 eyes in 2% dextran solution and 10 eyes in 20% dextran solution). CXL treatment was performed in 10 porcine eyes, the other 10 porcine eyes were regarded as a control group. Each eye was fixed on an experimental inflation platform to carry out Corvis measurements at different IOPs. Corneal biomechanical parameters were calculated based on Corvis measurement. Statistical analysis was used to analyze the influence of dextran solution and CXL on corneal biomechanical parameters based on Corvis parameters. Results: Corneal energy absorbed area (Aabsorbed) decreased after being soaked in dextran solution under IOP of 15 mmHg; Corneal elastic modulus (E) decreased after being soaked in 2% dextran solution and increased after being soaked in 20% dextran solution; SP-A1 increased after CXL. Conclusion: Both dextran solution and CXL can change corneal biomechanical properties; SP-A1 may be used as an effective parameter for the evaluation of CXL.

scholarly journals Evaluaton of the Use of Bone Implants as a Therapy for Deep Defects in the Parodoncium

2014 ◽  
Vol 15 (2) ◽  
pp. 79-82
Author(s):  
Momir Stevanović ◽  
Dušica Ćirić
Keyword(s):  
Tricalcium Phosphate ◽  
Periodontal Regeneration ◽  
Clinical Problem ◽  
Periodontal Pocket ◽  
Control Group ◽  
Lower Jaw ◽  
Standard Application ◽  
Inflammatory Processes ◽  
Infrabony Defects ◽  
And Control

ABSTRACT Reconstruction of infrabony defects created by the chronic inflammatory processes of parodontopathy represents a major clinical problem in paradontology. The objective of this research was to compare and evaluate the efficiency of two bone substituents at reversing the deep infrabony defects in the paradentium after the application of a new “Biohapel” material consisting of biphasic calcium phosphate/poly DL-lactide-co-glycolide. This study included 12 patients of both genders with an average age of 49.7 years who were suffering from clinically manifested parodontopathy. The main criteria for selecting patients were the presence of six similar infrabony periodontal defects that were at least 4 mm deep and in the lower side of the teeth on both sides of the jaw. After applying a basic causal parodontopathy treatment, defects were divided into two groups, including an experimental group (n=72) in which defects were reconstructed using the “Biohapel” bone substituent (on one side of the lower jaw) and a control group (n=72) in which infrabony defects were reconstructed using β tricalcium phosphate (βTCP) (CerasorbR), a preparation very commonly applied in regenerative parodontopathy treatment (on the other side of the lower jaw). Markers to assess treatment efficacy were determined before surgery and 6 months after surgery by measuring the depth of periodontal pocket and the level of the junctional epithelium. We observed statistically significant differences in the periodontal regeneration markers between the experimental and control groups (p<0.05). Application of “Biohapel” during the surgical treatment of infrabony defects in the paradentium significantly improves the bone regeneration of the paradentium when compared with the standard application of β-tricalcium phosphate .

scholarly journals Effect of implants of hydroxyapatite with tricalcium phosphates alloyed with Si on histomorphological and biochemical parameters in cases of bone defects of rabbits

2021 ◽  
Vol 12 (2) ◽  
pp. 281-288
Author(s):  
V. O. Chemerovskiy ◽  
M. V. Rublenko ◽  
S. V. Rublenko ◽  
N. V. Ulanchych ◽  
S. O. Firstov ◽  
...  
Keyword(s):  
General Anesthesia ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Biochemical Parameters ◽  
Bone Defects ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Spongy Bone ◽  
Diaphyseal Bone ◽  
Bone Trabeculae

Complex comminuted fractures are accompanied by development of bone defects and loss of reparative potential of the bone tissue in the region of the trauma. This brings the necessity of using implants with optimum osteoconductive and osteointegration properties. The objective of the study was determining the condition of biochemical bone markers and peculiarities of histomorphological changes under the influence of ceramic hydroxyapatite (HA) implants with various physical-chemical properties in the conditions of diaphyseal bone defects in rabbits. We composed control and experimental groups of rabbits with 10 individuals in each with diaphyseal bone defects (3 mm) of the radial bones formed under general anesthesia. In one experimental group, they were filled with granules of hydroxyapatite with α-tricalcium phosphate, and in the second group – with β-tricalcium phosphate, alloyed with Si. In the control rabbits, the defects healed under a blood clot. Blood was analyzed on the 3rd, 7th, 14th, 21st and 42nd days, and as reference we used biochemical parameters of blood of clinically healthy rabbits (n = 10). Bone biopsied materials were taken on days 21–42 under general anesthesia. When using hydroxyapatite with β-tricalcium phosphate, alloyed with Si, we determined early intensification of the levels of nitrogen oxide, angiogenesis and development of bone regenerate in conditions of shortening of inflammatory resorption phase, which was verified according to the level of tartrate-resistant acid phosphatase. According to the level of bone isoenzyme of alkaline phosphatase in the blood serum of animals of the control group, the reparative osteogenesis developed slowly and peaked on day 42, whereas in animals implanted with α-tricalcium phosphate, its development peaked peaked on days 14–42, and when using Si-alloy – on days 7–14. Histomorphologically, on the 21st day, in the case of replacement of bone defect with hydroxyapatite with α-tricalcium phosphate, coarse-fibered type of bone regenerate developed with no dense contact with the elements of the regenerate, while spongy bone trabeculae occurred when hydroxyapatite was applied with β-tricalcium phosphate alloyed with Si, and the control rabbits were observed to be in the stage of cartilaginous callus. On the 42nd day, under the influence of implants of hydroxyapatite with α-tricalcium phosphate, the spongy bone tissue transformed into compact tissue with further mineralization. With implants alloyed with Si, there occurred compact bone tissue, and bone regenerates of the control animals were regions of coarse-fibered and spongy bone tissue without dense contact with the parent bone. This study revealed that hydroxyapatite with β-tricalcium phosphate alloyed with Si had notable osteoinductive and osteointegrating properties, as indicated by early angiogenesis and osteoblast reaction, positive dynamics of the marker biochemical parameters with faster and better development of bone regenerate as spongy bone trabeculae.

scholarly journals Histomorphological characteristics of bone replacement in rabbits with hydroxyapatite ceramics and Platelet-Rich Fibrin

2021 ◽  
Vol 23 (102) ◽  
pp. 43-52
Author(s):  
S. М. Shevchenko ◽  
M. V. Rublenko ◽  
N. V. Ulyanchich ◽  
P. P. Klymenko
Keyword(s):  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Blood Clot ◽  
Compact Bone ◽  
Control Group ◽  
Bone Lesions ◽  
Platelet Rich Fibrin ◽  
Reparative Osteogenesis ◽  
Hydroxyapatite Ceramics ◽  
Experimental Group

The results of a histomorphological study of bone regenerates in rabbits after osteosubstitution with platelet-rich fibrin and its combination with hydroxyapatite granules with β-tricalcium phosphate in spongy and compact bone tissue at different periods of reparative osteogenesis are presented. Three groups of rabbits were formed. In the control group, the defects were left to heal under the blood clot. Bone lesions in the first experimental group were filled with platelet-rich fibrin, in the second – with a combination of platelet-rich fibrin and hydroxyapatite with β-tricalcium phosphate. All animals were kept in the same conditions of feeding and housing, had unlimited access to water. During the study, the rabbits were monitored clinically. The animals were taken out of the experiment on the 14th, 21st and 42nd days, samples of bone tissue were taken, they were fixed, decalcified, dehydrated in alcohols of increasing concentration and embedded in paraffin. In the presented study, it is most likely that the newly formed bone tissue is formed precisely due to osteoinduction in the experimental groups. When using granules of hydroxyapatite and β-tricalcium phosphate with platelet-rich fibrin, bone regenerate is between the composite granules and is not associated with contact with the maternal bone. Its cells appear in different places of the defect. In the spongy bone tissue on the 21st day, regeneration proceeded more fully and faster in the second experimental group, as evidenced by the significant density of cells of the osteoblastic row, the thickness of the bone trabeculae and their volume, filling the site of the defect. On the 42nd day, in the second experimental group, when using a combination of autobiomaterial and hydroxyapatite granules with β-tricalcium phosphate, the regenerate contained a significantly larger number of osteogenic cells in the thickness of the trabeculae, which indicated a more intensive course of reparative osteogenesis in comparison with the first experimental and control groups. In the compact bone on day 21, regeneration was more complete and faster in the second experimental group. On the 42nd day, according to the degree of maturity of the bone regenerate, calcium-phosphate ceramics in combination with platelet-rich fibrin optimizes reparative osteogenesis most clearly. According to the degree of intensity of the osteoregeneration process, the groups can be placed in the following sequence: control ˂PRF˂PRF+HA/β-TCP–700. According to histomorphological characteristics, the combination of hydroxyapatite with β-tricalcium phosphate and platelet-rich fibrin gives greater osteoinduction to the composite material, which is confirmed by the high cell density, namely of osteoblasts and osteocytes. The use of PRF in combination with other materials may become promising for the correction of reparative osteogenesis in conditions of limited or reduced regenerative potential of bone tissue.

The Histomorphometry of Rabbit Bone Tissue with Experimental Osteoporosis after Implantation of Biphasic Calcium Phosphate Materials

2020 ◽  
Vol 850 ◽  
pp. 249-253
Author(s):  
Vladislavs Ananjevs ◽  
Arnis Abolins ◽  
Janis Locs ◽  
Ilze Salma ◽  
Andrejs Skagers ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Trabecular Bone ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Bone Area ◽  
Calcium Phosphate Ceramic ◽  
Trochanter Major ◽  
Lower Jaw ◽  
Trabecular Bone Area ◽  
Rabbit Bone

The histomorphometry of the rabbit bone tissue from the lower jaw was done. Authors hypothesized that local enhancement with biphasic calcium phosphate ceramic materials in the femur trochanter major area increase the trabecular bone volume outside the implantation zone in vivo. Twenty-two California female rabbits were included in this study and were divided into four groups. Four healthy rabbits composed a control group (A group), while other eighteen underwent ovariectomy. Bone defects were created in femur trochanter major region. Sham surgery group (B group) consisted of four female rabbits with osteoporosis and bone defect, but no biomaterials were implanted. In C group (seven rabbits) created defects were filled with granules of biphasic calcium phosphate ceramic (hydroxyapatite (HAP) and tricalcium phosphate (TCP) 30/70); in D group (seven rabbits) defects were filled with the same granules (HAP/TCP 30/70) together with strontium (5% by mass). Twenty-two bone samples were taken from lower jaw premolar region. Trabecular bone area was measured using Image Pro Plus 7 program, where three equal fields (0.975 mm2) of view were at random chosen in all bone samples. Results have shown that the trabecular bone area in A group was 0.201 mm2 (0.176-0.233), which is statistically significantly higher (p <0.0001) than in B group 0.127 mm2 (0.118 – 0.149), C group 0.136 mm2 (0.108 – 0.166) and D group 0.135 mm2 (0.126 – 0.164), respectively. Statistically significant differences between B, C and D groups were not found (p > 0.05).

Sign in / Sign up

Export Citation Format

Share Document