Hypoxia Marker GLUT-1 (Glucose Transporter 1) is an Independent Prognostic Factor for Survival in Bladder Cancer Patients Treated with Radical Cystectomy

BackgroundBladder cancer is the 10th most common cancer and most common urothelial malignancy worldwide. Prognostic biomarkers for bladder cancer patients are required for individualized treatment. Monocarboxylate transporter 4 (MCT4), encoded by SLC16A3 gene, is a potential biomarker for bladder cancer because of its crucial role in the lactate efflux in the aerobic glycolysis process. We aimed to study the association between MCT4 expression and the overall survival (OS) of bladder cancer patients.MethodsThe published single-cell RNA sequencing data of 49,869 bladder cancer cells and 15,827 normal bladder mucosa cells and The Cancer Genome Atlas (TCGA) bladder cancer cohort data were used to explore the mRNA expression of SLC16A3 in bladder cancer. Eighty-nine consecutive bladder cancer patients who had undergone radical cystectomy were enrolled as a validation cohort. The expression of MCT4 proteins in bladder cancer specimens was detected using immunohistochemistry staining. The Kaplan–Meier survival analysis and Cox regression were performed to analyze the association between MCT4 protein expression and OS in bladder cancer patients.ResultsSLC16A3 mRNA was upregulated in bladder cancer cells. The upregulated genes in SLC16A3-positive epithelial cells were enriched in the glycolysis process pathway and monocarboxylic acid metabolic process pathway. Patients with high SLC16A3 mRNA expression showed significantly poor OS (p = 0.016). High MCT4 protein expression was also found to be an independent predictor for poor OS in bladder cancer patients (HR: 2.462; 95% CI: 1.202~5.042, p = 0.014). A nomogram was built based on the results of the multivariate Cox analysis.ConclusionBladder cancer with high SLC16A3 mRNA expression has a poor OS. High MCT4 protein expression is an independent prognostic factor for bladder cancer patients who had undergone radical cystectomy.

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MP38-02 THE AMOUNT OF INTRA- AND POSTOPERATIVE BLOOD TRANSFUSION IN BLADDER CANCER PATIENTS WITH RADICAL CYSTECTOMY IS AN INDEPENDENT PROGNOSTIC FACTOR FOR POOR OUTCOME – RESULTS OF A SINGLE-CENTER STUDY

The Journal of Urology ◽

10.1016/j.juro.2016.02.106 ◽

2016 ◽

Vol 195 (4S) ◽

Author(s):

Alexander Buchner ◽

Tobias Grimm ◽

Birte-Swantje Schneevoigt ◽

Alexander Kretschmer ◽

Markus Grabbert ◽

...

Keyword(s):

Bladder Cancer ◽

Prognostic Factor ◽

Blood Transfusion ◽

Cancer Patients ◽

Radical Cystectomy ◽

Independent Prognostic Factor ◽

Single Center ◽

Postoperative Blood Transfusion ◽

Single Center Study ◽

Center Study

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Gender as an independent prognostic factor for final tumor stage and overall survival in patients with muscle invasive bladder cancer treated with radical cystectomy

European Urology Supplements ◽

10.1016/s1569-9056(16)15048-1 ◽

2016 ◽

Vol 15 (5) ◽

pp. e1220

Author(s):

D. Gajewska ◽

A. Lemiński ◽

K. Kaczmarek ◽

J. Lisiński ◽

M. Puszyński ◽

...

Keyword(s):

Overall Survival ◽

Bladder Cancer ◽

Prognostic Factor ◽

Radical Cystectomy ◽

Tumor Stage ◽

Independent Prognostic Factor ◽

Invasive Bladder Cancer ◽

Muscle Invasive Bladder Cancer ◽

Muscle Invasive

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1980 LYMPH NODE METASTASES ABOVE THE ILIAC BIFURCATION AS AN INDEPENDENT PROGNOSTIC FACTOR IN BLADDER CANCER PATIENTS UNDERGOING RADICAL CYSTECTOMY WITH EXTENDED LYMPH NODE DISSECTION

The Journal of Urology ◽

10.1016/j.juro.2011.02.2206 ◽

2011 ◽

Vol 185 (4S) ◽

Author(s):

Ryan Dorin ◽

Pascal Zehnder ◽

Manuel Eisenberg ◽

Jie Cai ◽

Gus Miranda ◽

...

Keyword(s):

Bladder Cancer ◽

Lymph Node ◽

Prognostic Factor ◽

Cancer Patients ◽

Radical Cystectomy ◽

Lymph Node Metastases ◽

Node Dissection ◽

Extended Lymph Node Dissection ◽

Node Metastases ◽

Iliac Bifurcation

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MicroRNA-340 Inhibits Bladder Cancer Cell Growth by Downregulating Glucose Transporter-1

10.21203/rs.3.rs-48508/v1 ◽

2020 ◽

Author(s):

Junlong Li ◽

Gang Xu ◽

Shouhua Pan

Keyword(s):

Cell Proliferation ◽

Bladder Cancer ◽

Gene Networks ◽

Glucose Transporter ◽

Inverse Correlation ◽

Western Blots ◽

Tissue Samples ◽

Glucose Transporter 1 ◽

Glut 1 ◽

Bladder Cell

Abstract Background: MicroRNA (miRNA)-340 is emerging as a critical regulator for the development and progression of various cancers, such as oral squamous cell carcinoma and prostate cancer. However, little was known about the role of miR-340 in bladder cancer. Methods: Bladder cancer and adjacent non-tumor tissue samples were collected from thirty patients undergoing surgical resection at Shaoxing People's Hospital. The expression of miR-340 and Glut-1 were studied via real-time quantitative PCR (RT-qPCR) or western blots. Bladder cancer T24 cells were transfected with miR-340 mimics to explore cell proliferation and apoptosis via MTT assay, flow cytometer, RT-qPCR and western blots. Results: Compared with that of normal tissues, miR-340 expression was significantly lower, while both mRNA and protein expression of Glut-1 were higher in bladder cancer tissues. The miR-340 could negatively regulate Glut-1 expression in bladder cells. Moreover, bladder cell proliferation could be inhibited by miR-340 and the corresponding antitumor effect could partially reverse by the overexpression of Glut-1. In view of the complexity of gene networks, some other multiple pathways might also confer to miR-340 inducing bladder cell apoptosis, including downregulating PCNA, upregulating Bax and decreasing the phosphorylation levels of PI3K and AKT.Conclusion: This work suggested an inverse correlation between miR-340 and glucose transporter-1 (Glut-1) expression in bladder cancer. miR-340/Glut-1 axis might be a potential and novel therapeutic target for the treatment of bladder cancer. More investigations need to further explore the applications of miR-340 in bladder cancer.

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Comparison of Preoperative Neutrophil-Lymphocyte and Platelet-Lymphocyte Ratios in Bladder Cancer Patients Undergoing Radical Cystectomy

BioMed Research International ◽

10.1155/2019/3628384 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Ruiliang Wang ◽

Yang Yan ◽

Shenghua Liu ◽

Xudong Yao

Keyword(s):

Bladder Cancer ◽

Prognostic Factors ◽

Prognostic Factor ◽

Receiver Operating Characteristic ◽

Cancer Patients ◽

Radical Cystectomy ◽

Prognostic Value ◽

Operating Characteristic ◽

Receiver Operating Characteristic Curves ◽

Lymphocyte Ratio

Introduction. Neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) have been proven to be significant prognostic factors in many cancers. We aimed to retrospectively investigate the prognostic value of NLR and PLR in patients with bladder cancer undergoing radical cystectomy. Materials and Methods. The study comprised patients from 2010 to 2018 who were diagnosed with bladder cancer and received radical cystectomy. Clinical and pathological parameters were collected. Receiver operating characteristic curves of NLR and PLR were plotted for overall survival (OS) and cancer-specific survival (CSS). The best cutoff value of NLR and PLR were determined using X-tile software. The prognostic value of NLR and PLR for OS and CSS was analyzed using the Kaplan–Meier method and Cox regression models. Results. A total of 223 patients were enrolled with a medium follow-up period of 57 months. Receiver operating characteristic curves showed that PLR was superior to NLR as a prognostic factor in patients with bladder cancer undergoing radical cystectomy. Univariate analysis revealed that NLR (p=0.032 and p=0.041) and PLR (p=0.003 and p=0.003) were significantly associated with both OS and CSS, respectively. Multivariate analysis identified only PLR as independent prognostic factors for OS (p=0.046) and CSS (p=0.039), respectively. Conclusions. The present findings suggested that compared with NLR, PLR was a superior prognostic factor of OS and CSS in bladder cancer patients indicated to radical cystectomy.

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MP61-08 PROGRESSION OF T1 HIGH RISK INTO MUSCLE-INVASIVE BLADDER CANCER IS AN INDEPENDENT PROGNOSTIC FACTOR OF MORTALITY AFTER RADICAL CYSTECTOMY

The Journal of Urology ◽

10.1016/j.juro.2014.02.1885 ◽

2014 ◽

Vol 191 (4S) ◽

Author(s):

Federico Pellucchi ◽

Marco Moschini ◽

Lorenzo Rocchini ◽

Giovanni La Croce ◽

Vito Cucchiara ◽

...

Keyword(s):

Bladder Cancer ◽

High Risk ◽

Prognostic Factor ◽

Radical Cystectomy ◽

Independent Prognostic Factor ◽

Invasive Bladder Cancer ◽

Muscle Invasive Bladder Cancer ◽

Muscle Invasive

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The Role of Hypoxia-Inducible Factor-1α, Glucose Transporter-1, (GLUT-1) and Carbon Anhydrase IX in Endometrial Cancer Patients

BioMed Research International ◽

10.1155/2014/616850 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 21

Author(s):

Pawel Sadlecki ◽

Magdalena Bodnar ◽

Marek Grabiec ◽

Andrzej Marszalek ◽

Pawel Walentowicz ◽

...

Keyword(s):

Endometrial Cancer ◽

Cancer Patients ◽

Glucose Transporter ◽

Hypoxia Inducible Factor ◽

Clinicopathological Features ◽

Glucose Transporter 1 ◽

Histologic Subtype ◽

Risk Of Recurrence ◽

Glut 1

Hypoxia-inducible factor-1α(HIF-1α), glucose transporter-1 (GLUT-1), and carbon anhydrase IX (CAIX) are important molecules that allow adaptation to hypoxic environments. The aim of our study was to investigate the correlation between HIF-1α, GLUT-1, and CAIX protein level with the clinicopathological features of endometrial cancer patients.Materials and Methods. 92 endometrial cancer patients, aged 37–84, were enrolled to our study. In all patients clinical stage, histologic grade, myometrial invasion, lymph node, and distant metastases were determined. Moreover, the survival time was assessed. Immunohistochemical analyses were performed on archive formalin fixed paraffin embedded tissue sections.Results. High significant differences(P=0.0115)were reported between HIF-1αexpression and the histologic subtype of cancer. Higher HIF-1αexpression was associated with the higher risk of recurrence(P=0.0434). The results of GLUT-1 and CAIX expression did not reveal any significant differences between the proteins expression in the primary tumor and the clinicopathological features.Conclusion. The important role of HIF-1αin the group of patients with the high risk of recurrence and the negative histologic subtype of the tumor suggest that the expression of this factor might be useful in the panel of accessory pathomorphological tests and could be helpful in establishing more accurate prognosis in endometrial cancer patients.

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