scholarly journals Determination of the total content of flavonoids in herbal mixtures with anti-diabetic activity

2021 ◽  
pp. 77-84
Author(s):  
А. О. Савич ◽  
Л. С. Криськів ◽  
Л. М. Мосула
Keyword(s):  
Diabetes Mellitus ◽  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Total Flavonoid ◽  
Hydroxyl Groups ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Herbal Mixture ◽  
Herbal Mixtures

Diabetes mellitus is an important social and medical problem, as it causes the development of dangerous complications that lead to disability and mortality. This disease is characterized by a multi-vector pathogenesis that requires a comprehensive approach to treatment. Due to the use of mixtures of medicinal plants in the treatment of diabetes, it is possible to cover all aspects of the development of this disease and its complications, because the combination of different medicinal plants contain more biologically active substances that affect all parts of the pathogenetic mechanism of diabetes mellitus and its complications. Plant biocompounds have a wide range of pharmacological action and various mechanisms of influence on the development of diabetes and diabetic angiopathies (inactivation of the antioxidant defense system, activation of lipid peroxidation and the development of oxidative stress). In addition, the niche of the pharmaceutical market of Ukraine with phytomixtures is almost empty. The aim of the work was to study the total flavonoid content in some herbal mixtures, which have established hypoglycemic, hypolipidemic and antioxidant activity in previous studies in vivo The study of the total flavonoid content in herbal samples was carried out by UV-spectrophotometry with the recording of the absorption spectrum of complexation product of flavonoids with aluminum chloride (λmax 415 nm) in terms of rutin. According to the results of UV-spectrophotometric determination, it was found that the total flavonoid content in the herbal mixture No 3 was 1.47 ± 0.04%, in the herbal mixture No 4 – 1.78 ± 0.04%, in the herbal mixture No 7 – 1.29 ± 0.02%, in the herbal mixture No 13 – 1.16 ± 0.02% and in the herbal mixture No 19 – 3.62 ± 0.06% in terms of rutin. It was found that the best flavonoid content (3.62 ± 0.06%) was found in the plant mixture № 19, which contains nettle leaves, dandelion roots, blueberry leaves, rose hips and peppermint herb. Phytochemical studies may indicate a correlation between the content of flavonoids and pharmacological properties of the studied mixtures, because the functional hydroxyl groups of the latter cause strong antioxidant activity by neutralizing free radicals, chelation of heavy metal ions, increasing the activity of antioxidant protection products, which has been confirmed in previous in vivo and in vitro studies.

scholarly journals Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi Kosterm.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Alifni Adha Bakti ◽  
Liling Triyasmono ◽  
Muhammad Ikhwan Rizki
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Natural Resources ◽  
Experimental Research ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Anti Oxidant

ABSTRAK Bahan alam dapat dijadikan bahan obat baru karena mengadung metabolit sekunder. Di Indonesia terdapat lebih kurang 30.000 jenis tumbuh-tumbuhan yang mengandung metabolit sekunder, lebih kurang 7.500 jenis diantaranya termasuk tanaman berkhasiat obat. Salah satunya adalah tanaman kasturi (Mangifera casturi Kosterm.). Tanaman M. casturi merupakan tumbuhan khas Kalimantan Selatan yang hanya dimanfaatkan oleh masyarakat untuk dikonsumsi, tidak untuk pengobatan. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dan aktivitas antioksidan dari ekstrak etanol daun M. casturi. Penelitian ini bersifat non-eksperimental. Sampel yang digunakan adalah daun M. casturi yang berasal dari Kabupaten Banjar, Kalimantan Selatan. Penentuan Kadar flavonoid total dilakukan secara spektrofotometri UV-Vis dengan pereaksi kompleks AlCl3 sedangkan aktivitas antioksidan ditentukan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil penelitian diperoleh kadar flavonoid total ekstrak etanol daun M. casturi sebesar 9,31 ± 0,08 %b/b dan aktivitas antioksidan dengan nilai IC50 sebesar 34,558 ppm sehingga termasuk dalam kategori antioksidan yang sangat aktif. Kata kunci: Antioksidan, DPPH, Flavonoid Total, Mangifera casturi. ABSTRACT Natural resources can be used as the new medicine ingridients because it has second metabolite. In Indonesia, there are more than 30.000 kinds of plants that contain second metabolite, more or less than 7.500 kinds of those are medicinal plants. One of those plants is Kasturi (Mangifera casturi Kosterm.). M. casturi is a typical plant from South Kalimantan that be used as a food not as a medicine. The purpose of this research is to determine the total of flavonoid content and anti-oxidant activity from ethanol extract of M. casturi leaves. This study is a non-experimental research. Sample which used in this research is M. casturi Leaves from Banjar Region, South Kalimantan. The research for total Flavonoid content is done by UV-Vis spectrophotometric with AlCl3 reagent complex while the anti-oxidant activity is determined by DPPH (2,2-difenil-1-pikrilhidrazil) method. The result of this research are extract of M. casturi leaves obtains 9,31 ± 0,08 %b/b of total flavonoid and the antioxidant activity result with IC50 value is 34,558 ppm, so it can be categorized as a very active anti-oxidant. Keywords: Antioxidant, DPPH, Total Flavonoid, Mangifera casturi.

scholarly journals Antioxidant Activity of Dry Birch (Betula Pendula) Leaves Extract

Folia Medica ◽  
2018 ◽  
Vol 60 (4) ◽  
pp. 571-579 ◽  
Author(s):  
Dimitar Penkov ◽  
Velichka Andonova ◽  
Delian Delev ◽  
Ilia Kostadinov ◽  
Margarita Kassarova
Keyword(s):  
Antioxidant Activity ◽  
Betula Pendula ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Albino Rats ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Abstract Background: Betula pendula is widespread in Europe and Asia. It has been used in traditional medicine since ancient times. Birch leaf extracts are known to exhibit a number of pharmacological activities. Antioxidant activity has also been reported. Aim: The aim of this work was to investigate the antioxidant activity of a dry leaf extract from Betula pendula Roth. Materials and methods: The total flavonoid content was determined. Some of the most commonly used methods were applied to evaluate the antioxidant capacity of the extract in vitro and in vivo. The ability of the extract to scavenge DPPH free radicals was evaluated by the method described by Brand-Williams with suitable modifications. ABTS decolorization assay was also applied. The in vivo assay was performed after acute and chronic administration of the extract into white albino rats, in a dose of 100 and 500 mg/kg bw. The antioxidant potential of the plasma was determined using FRAP reagent. Results: A total flavonoid content of 42.5 mg/g was found, expressed as quercetin. The antioxidant activity against ABTS was concentration and time dependent. For example the concentration of 200 μg/ml led to 70.95% – 99.46% scavenging activity. DPPH scavenging activity was found to be about 98% at a concentration of 80 μg/ml. The extract possesses antioxidant potential, comparable with that of Trolox, in acute application. In chronic application, poorer results are observed, probably due to biotransformation and elimination processes. Conclusion: Dried birch leaf extract has a relatively high antioxidant potential and could be used as a natural source of antioxidants.

scholarly journals Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Aatika Sikandar ◽  
Mengyue Zhang ◽  
Yuanyuan Wang ◽  
Xiaofeng Zhu ◽  
Xiaoyu Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Penicillium Chrysogenum ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Pharmaceutical Industries

Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.

scholarly journals Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Thandiwe Alide ◽  
Phanice Wangila ◽  
Ambrose Kiprop
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Cooking Temperature ◽  
Ethanolic Extracts ◽  
The Mean

Abstract Objective To investigate the effect of cooking temperature and time on the total phenolic content, total flavonoid content and antioxidant activity of aqueous and ethanolic extracts of garlic. Results The mean total phenolic content of fresh garlic were 303.07 ± 6.58 mg gallic acid equivalent per 100 g (GAE/100 g) and 638.96 ± 15.30 mg GAE/100 g of plant material for the aqueous and ethanolic extracts respectively. The mean total flavonoid content 109.78 ± 6.78 mg quercetin equivalent per 100 g (QE/100 g) and 258.47 ± 12.37 QE/100 g for aqueous and ethanolic extracts respectively. Fourier transform infrared spectral data showed absorptions in the range for carboxylic acids, hydroxyl group, esters, and alcohols, confirming the presence of phenols and flavonoids in the extracts. Cooking temperature had a significant effect on total phenolic content and total flavonoid content while cooking time did not have a significant effect on the phytochemicals and antioxidant activity.

scholarly journals Antioxidative Activities and Active Compounds of Extracts fromCatalpaPlant Leaves

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hongyu Xu ◽  
Gege Hu ◽  
Juane Dong ◽  
Qin Wei ◽  
Hongbo Shao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Crude Extracts ◽  
Catalpa Ovata ◽  
High Antioxidant Activity ◽  
Water Fractions

In order to screen theCatalpaplant with high antioxidant activity and confirm the corresponding active fractions fromCatalpa ovataG. Don,C. fargesiiBur., andC. bungeiC. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions ofCatalpaplant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content inC. bungeiC. A. Mey. (30.07 mg/g·DW) was the highest, followed by those inC. fargesiiBur. (25.55 mg/g·DW) andC. ovataG. Don (24.96 mg/g·DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves ofC. bungeiC. A. Mey., the total flavonoid content and antioxidant activity in crude extracts fromC. bungeiC. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed byn-butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin (1) and apigenin (2), respectively.

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