scholarly journals Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi Kosterm.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Alifni Adha Bakti ◽  
Liling Triyasmono ◽  
Muhammad Ikhwan Rizki
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Natural Resources ◽  
Experimental Research ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Anti Oxidant

ABSTRAK Bahan alam dapat dijadikan bahan obat baru karena mengadung metabolit sekunder. Di Indonesia terdapat lebih kurang 30.000 jenis tumbuh-tumbuhan yang mengandung metabolit sekunder, lebih kurang 7.500 jenis diantaranya termasuk tanaman berkhasiat obat. Salah satunya adalah tanaman kasturi (Mangifera casturi Kosterm.). Tanaman M. casturi merupakan tumbuhan khas Kalimantan Selatan yang hanya dimanfaatkan oleh masyarakat untuk dikonsumsi, tidak untuk pengobatan. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dan aktivitas antioksidan dari ekstrak etanol daun M. casturi. Penelitian ini bersifat non-eksperimental. Sampel yang digunakan adalah daun M. casturi yang berasal dari Kabupaten Banjar, Kalimantan Selatan. Penentuan Kadar flavonoid total dilakukan secara spektrofotometri UV-Vis dengan pereaksi kompleks AlCl3 sedangkan aktivitas antioksidan ditentukan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil penelitian diperoleh kadar flavonoid total ekstrak etanol daun M. casturi sebesar 9,31 ± 0,08 %b/b dan aktivitas antioksidan dengan nilai IC50 sebesar 34,558 ppm sehingga termasuk dalam kategori antioksidan yang sangat aktif. Kata kunci: Antioksidan, DPPH, Flavonoid Total, Mangifera casturi. ABSTRACT Natural resources can be used as the new medicine ingridients because it has second metabolite. In Indonesia, there are more than 30.000 kinds of plants that contain second metabolite, more or less than 7.500 kinds of those are medicinal plants. One of those plants is Kasturi (Mangifera casturi Kosterm.). M. casturi is a typical plant from South Kalimantan that be used as a food not as a medicine. The purpose of this research is to determine the total of flavonoid content and anti-oxidant activity from ethanol extract of M. casturi leaves. This study is a non-experimental research. Sample which used in this research is M. casturi Leaves from Banjar Region, South Kalimantan. The research for total Flavonoid content is done by UV-Vis spectrophotometric with AlCl3 reagent complex while the anti-oxidant activity is determined by DPPH (2,2-difenil-1-pikrilhidrazil) method. The result of this research are extract of M. casturi leaves obtains 9,31 ± 0,08 %b/b of total flavonoid and the antioxidant activity result with IC50 value is 34,558 ppm, so it can be categorized as a very active anti-oxidant. Keywords: Antioxidant, DPPH, Total Flavonoid, Mangifera casturi.

scholarly journals Determination of the total content of flavonoids in herbal mixtures with anti-diabetic activity

2021 ◽  
pp. 77-84
Author(s):  
А. О. Савич ◽  
Л. С. Криськів ◽  
Л. М. Мосула
Keyword(s):  
Diabetes Mellitus ◽  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Total Flavonoid ◽  
Hydroxyl Groups ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Herbal Mixture ◽  
Herbal Mixtures

Diabetes mellitus is an important social and medical problem, as it causes the development of dangerous complications that lead to disability and mortality. This disease is characterized by a multi-vector pathogenesis that requires a comprehensive approach to treatment. Due to the use of mixtures of medicinal plants in the treatment of diabetes, it is possible to cover all aspects of the development of this disease and its complications, because the combination of different medicinal plants contain more biologically active substances that affect all parts of the pathogenetic mechanism of diabetes mellitus and its complications. Plant biocompounds have a wide range of pharmacological action and various mechanisms of influence on the development of diabetes and diabetic angiopathies (inactivation of the antioxidant defense system, activation of lipid peroxidation and the development of oxidative stress). In addition, the niche of the pharmaceutical market of Ukraine with phytomixtures is almost empty. The aim of the work was to study the total flavonoid content in some herbal mixtures, which have established hypoglycemic, hypolipidemic and antioxidant activity in previous studies in vivo The study of the total flavonoid content in herbal samples was carried out by UV-spectrophotometry with the recording of the absorption spectrum of complexation product of flavonoids with aluminum chloride (λmax 415 nm) in terms of rutin. According to the results of UV-spectrophotometric determination, it was found that the total flavonoid content in the herbal mixture No 3 was 1.47 ± 0.04%, in the herbal mixture No 4 – 1.78 ± 0.04%, in the herbal mixture No 7 – 1.29 ± 0.02%, in the herbal mixture No 13 – 1.16 ± 0.02% and in the herbal mixture No 19 – 3.62 ± 0.06% in terms of rutin. It was found that the best flavonoid content (3.62 ± 0.06%) was found in the plant mixture № 19, which contains nettle leaves, dandelion roots, blueberry leaves, rose hips and peppermint herb. Phytochemical studies may indicate a correlation between the content of flavonoids and pharmacological properties of the studied mixtures, because the functional hydroxyl groups of the latter cause strong antioxidant activity by neutralizing free radicals, chelation of heavy metal ions, increasing the activity of antioxidant protection products, which has been confirmed in previous in vivo and in vitro studies.

scholarly journals Total Flavonoid Levels of Ethanol Extract of Lemongrass Leaves and Thatch Grass Leaves Using UV-VIS Spectrophotometry

2018 ◽  
pp. 337-345
Author(s):  
Yudhi Nuryadin ◽  
Tadjuddin Naid ◽  
Andi Amaliah Dahlia ◽  
Seniwati Dali
Keyword(s):  
Quantitative Analysis ◽  
Medicinal Plants ◽  
Qualitative Analysis ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Natural Antioxidant ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Lemongrass Leaves (Cymbopogoncitratus DC.) and Thatch Grass Leaves (Imperata cylindrical (L.)Raeusch) are medicinal plants belong to similary family. Their activity as natural antioxidant sources because it’s contains the flavonoids compound. This study aimed to determine the total flavonoid content on the ethanol extract of lemongrass leaves and the thatch grass leaves using UV-Vis spectrophotometry. The extraction wasdone by maceration methodusing ethanol. The first sample is 3,95% lemongrass leaves, and the second one is 2,98% thatch grass leaves. The qualitative analysis by TLC method with the eluent ratio n-Hexane:ethyl acetate (6:4) showed that both samples positively contain flavonoids. The quantitative analysis by spectrophotometry at the maximum wavelength 431 nm showed that total flavonoid content in the first sample is 2,8666 % and the second sample is 3,4866 %.

scholarly journals PHYTOCHEMICAL PROFILING, ASSESSMENT OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF ETHNOMEDICINAL PLANT, MEYNA SPINOSA FROM ASSAM

2019 ◽  
pp. 61-63
Author(s):  
REKHA BORA ◽  
SEEMA KHAKHALARY ◽  
TAPAN DUTTA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Radical Scavenging Assay

Objective: The present study qualitative phytoconstituents examine the total phenol, total flavonoid content (TFC), and antioxidant efficiencies traditionally used plant, Meyna spinosa. Methods: Chemical profiling, estimation of total phenolic content (TPC), TFC, and antioxidant activity of ethanol extracts of M. spinosa have performed by applying standard protocols. Antioxidant activity of leaf and stem was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. TPC and TFC of the plant were assessed using Folin–Ciocalteu colorimetric and aluminum colorimetric assay, respectively. Results: The findings of the study exhibit that ethanol extract of M. spinosa is proved to be the presence of phytoconstituents (7/9) such as alkaloids, terpenoids, saponins, tannins, phytosterols, flavonoids, and phenolic compounds, while carbohydrate, fixed oils, and fats are unavailable. In addition, phenolic compositions of ethanol extract of leaf and stem; 93.21±2.93 and 54.33±0.69 mg gallic acid equivalents/g extract, respectively; TFCs of leaf and stem have recorded as 61.55±1.21 and 37.55±1.28 mg quercetin equivalents/g extract, respectively. Antioxidant efficiency of both leaf and stem is tested using DPPH radical scavenging assay as IC50 20.68±0.32 and 50.99±0.56 μg/ml, respectively. Conclusion: From the above results, it has concluded that the ethanol extract of the M. spinosa leaves and stems seizes rich phytoconstituents which can be applied in food technology, drug industries, ethnopharmacological fields, etc., for the development of healthiness and to battle against negative health consequences.

scholarly journals Aktivitas Antioksidan Dari Ekstrak Etanol Herba Poguntano (Picria Fel-Terrae Lour.) Secara In Vitro

2018 ◽  
Vol 1 (1) ◽  
pp. 218-223
Author(s):  
Nevi Hayati Utami ◽  
Suryadi Achamad ◽  
Panal Sitorus
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Latar Belakang: Antioksidan adalah senyawa yang mampu menangkal atau meredam efek negatif oksidan dalam tubuh, bekerja dengan cara mendonorkan satu elektronnya kepada senyawa yang bersifat oksidan sehingga aktivitas senyawa oksidan tersebut dapat dihambat. Herba poguntano (Picria fel-terrae Lour.) digunakan sebagai obat antidiabetes dan telah dilaporkan mengandung senyawa kimia yang terdiri dari alkaloid, saponin, flavonoid, dan polifenol yang merupakan metabolit sekunder yang bersifat antioksidan. Tujuan Penelitian: Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan serta mengetahui kandungan fenolat dan flavonoid total dari ekstrak etanol herba poguntano. Metode Penelitian: Ekstrak diperoleh secara maserasi dengan pelarut etanol 96%, lalu dipekatkan dengan alat rotary evaporator hingga didapatkan ekstrak kental. Ekstrak diuji dengan metode pemerangkapan radikal bebas DPPH (1,1-diphenyl-2-picrylhydrazyl) serta mengukur kandungan fenolat total dan flavonoid total dengan mengukur absorbansi menggunakan spektrofotometri uv-vis yang diinkubasi berturut-turut selama 60, 90 dan 40 menit pada panjang gelombang 516, 775 dan 432 nm. Hasil: Hasil pengukuran aktivitas antioksidan dengan metode pemerangkapan radikal bebas DPPH dari ekstrak etanol herba poguntano menunjukkan kekuatan antioksidan dalam kategori sangan kuat dengan nilai IC50 21.79 ppm dan kuersetin menunjukkan kekuatan antioksidan dalam kategori sangat kuat dengan nilai IC50 4.9 ppm. Hasil pengukuran kandungan fenolat total dengan metode Folin-Ciocalteau menunjukkan angka 66.29 mg GAE/g ekstrak serta hasil pengukuran kandungan flavonoid total menunjukkan angka 19.26 mg QE/g ekstrak. Kesimpulan: Ekstrak etanol herba poguntano mempunyai aktivitas antioksidan dengan kategori sangat kuat serta memiliki kandungan fenolat total yang tinggi dibandingkan dengan kandungan flavonoid total. Background: Antioxidants are compounds capable of counteracting or reducing the negative effects of oxidants in the body. Antioxidants work by donating an electron to an oxidant compound so that the activity of the oxidant compound can be inhibited. Poguntano (Picriafel-terrae Lour) is used as an antidiabetic drug and has been reported to contain chemical compounds, , as secondary metabolites that have antioxidant properties. Objective: This study aimed to determine antioxidant activity and total phenolic and flavonoid content of the ethanol extract of poguntano. Research Method: . The dried materials were macerated using 96% ethanol and then it was concentrated using rotary evaporator to obtain crude extract. The extract was tested by DPPH free radical scavenging method (1,1-diphenyl-2-picrylhydrazyl) and the total phenolic and flavonoid content was determinedby measuring absorbance using uv-vis spectrophotometry which was incubated successively for 60, 90 and 40 minutes at wavelength 516, 775 and 432 nm. Results: The evaluationof antioxidant activity using DPPH free radical scavenging method ofethanol extract of poguntano showed the antioxidant activitywas in very strong category with IC50 value of 21.79 ppm and quercetin showed the antioxidant activitywas in very strong category with IC50 value of 4.9 ppm. The result of the measurement of total phenolic content by the Folin-Ciocalteu method was66.29 mg GAE/g extract and the result of measurements of total flavonoid content was19.26 mg QE/g extract. Conclusion: Ethanol extract of poguntanohas antioxidant activity in a very strong category and it has higher total phenolic content compared with total flavonoid content.

scholarly journals KANDUNGAN SENYAWA FLAVONOID PADA DAUN MELINJO (Gnetum gnemon L.) BERDASARKAN PERBEDAAN TEMPAT TUMBUH

2017 ◽  
Vol 3 (2) ◽  
pp. 142-147
Author(s):  
Mersy T Tanamal ◽  
Pamella Mercy Papilaya ◽  
Alwi Smith
Keyword(s):  
Natural Resources ◽  
Total Flavonoid ◽  
Flavonoid Compound ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Gnetum Gnemon ◽  
The Village

Background: The village of Latuhalat and Kayu Putih village is a place that is on the beach and in the mountains. Potential natural resources are quite a lot of plants melinjo (Gnetum gnemon L). This study aims to determine the total compound of flavonoid content of leaf melinjo (Gnetum gnemon L) based on place difference. Method: The method used in this study using the method of Chang et al. The solvent used for diluting the melinjo extract was methanol solvent, then measured using a UV-VIS spectrophotometer. Result: Result of analysis of flavonoid compound content, Latuhalat Village has average total flavonoid content of13.080% and average total flavonoid content in Kayu Putih Village equal to 17.028%. Kayu Putih Village has more flavonoid compound than Latuhalat Village. Conclusion: Total levels of melinjo leaf flavonoids (Gnetum gnemon L) in Kayu Putih Village were more than total flavonoids of leaf melinjo (Gnetum gnemon L) in Latuhalat Village.

scholarly journals Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Aatika Sikandar ◽  
Mengyue Zhang ◽  
Yuanyuan Wang ◽  
Xiaofeng Zhu ◽  
Xiaoyu Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Penicillium Chrysogenum ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Pharmaceutical Industries

Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.

scholarly journals Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Thandiwe Alide ◽  
Phanice Wangila ◽  
Ambrose Kiprop
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Cooking Temperature ◽  
Ethanolic Extracts ◽  
The Mean

Abstract Objective To investigate the effect of cooking temperature and time on the total phenolic content, total flavonoid content and antioxidant activity of aqueous and ethanolic extracts of garlic. Results The mean total phenolic content of fresh garlic were 303.07 ± 6.58 mg gallic acid equivalent per 100 g (GAE/100 g) and 638.96 ± 15.30 mg GAE/100 g of plant material for the aqueous and ethanolic extracts respectively. The mean total flavonoid content 109.78 ± 6.78 mg quercetin equivalent per 100 g (QE/100 g) and 258.47 ± 12.37 QE/100 g for aqueous and ethanolic extracts respectively. Fourier transform infrared spectral data showed absorptions in the range for carboxylic acids, hydroxyl group, esters, and alcohols, confirming the presence of phenols and flavonoids in the extracts. Cooking temperature had a significant effect on total phenolic content and total flavonoid content while cooking time did not have a significant effect on the phytochemicals and antioxidant activity.

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