scholarly journals TOPICALITY OF FORENSIC MOLECULAR GENETIC EXAMINATION AND ISSUES REGARDING ITS PERFORMING

2018 ◽  
Vol 18 ◽  
pp. 256-263
Author(s):  
V. V. Topchiy
Keyword(s):  
Dna Analysis ◽  
Human Life ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Molecular Genetic Analysis ◽  
Present Stage ◽  
Law Enforcement Agencies ◽  
Biological Origin ◽  
Genetic Features ◽  
Genetic Examination

Modern progress in forensic molecular genetic examination allow to obtain information about a particular person using traces variety of biological origin especially while committing grave crimes against human life and health, that are usually found at the scene and belong to a human body. A significant advantage of this method under crime investigation is precisely the safe exclusion of suspected persons not involved in the commission of a crime, in identifying those who committed a crime with a high probability level. At the present stage of forensic molecular genetic examination development there are significant gaps in legislation that are solved by adopting relevant normative and legal acts and improving existing ones. Effective method for of DNA analysis development is the creation of appropriate bases of genetic features of a person. However, the legislative consolidation of this process should take place in the context of respecting and protecting personal rights. However, terms of performing molecular genetic examination significantly exceed the terms of pre-trial investigation. This problem can be solved by expanding network of laboratories that perform such examination. Despite presence of a small number of problems, it is possible to affirm that DNA analysis is the most effective and reliable of all known methods of person identification at the present stage. At present, expert molecular genetic analysis develops not only as a section of molecular genetic research but also as a complete element of criminalistic knowledge that is aimed at investigating and disclosing crimes. Therefore, implementation of molecular genetic research methods into the practice of law enforcement agencies in Ukraine will significantly increase investigation effectiveness of many serious crimes against person.

scholarly journals HUMAN BEING AS THE BEARER OF IDENTIFYING BIOLOGICAL INFORMATION

2021 ◽  
Author(s):  
L. Кotliarenko ◽  
А. Коfanov ◽  
O. Коfаnоvа ◽  
V. Zherebak
Keyword(s):  
Genetic Analysis ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Molecular Genetic Analysis ◽  
Genetic Identification ◽  
Criminal Proceedings ◽  
Genetic Traits ◽  
Criminal Offenses ◽  
Genetic Examination

In forensic practice, biological traces of a person are very often used as material evidence - blood, hair, saliva, semen, urine, sweat, as well as parts of organs and tissues. Establishing the origin of these traces from a specific person is very important for the investigation of criminal offenses. The current level of development of molecular genetic research indicates the need to use DNA analysis in the detection and investigation of criminal offenses against a person. Today, molecular genetic identification reveal reliable prospects for solving identification problems in the criminal proceedings and developing the evidence base, and also has a number of advantages over traditional serological methods for studying human biological traces. It should be noted that along with the traditional method of nuclear DNA research, mitochondrial DNA research is also being carried out, which allows solving the problem of molecular genetic examination to establish biological affinity. The value of this method lies in its effectiveness in the study of a small amount of degraded DNA, secretions and heavily damaged objects, the study of which is impossible by traditional methods. When performing a forensic molecular genetic examination for the full identification of the detected traces when examining the places of committed criminal offenses, comparative samples are important, as well as the selection of appropriate biological samples to establish paternity and family ties. Molecular genetic analysis of DNA is only one of the stages of identification, and in order to arrive at the final result, a statistical analysis of the data obtained is necessary, which is especially important when the genotypes of the criminal and the suspect in mixed tracks coincide. For a probable-statistical assessment of the results of the identification significance of the set of established genetic traits, the frequencies of the distribution of the studied alleles in the population are required. Today, the DNA analysis method has become one of the most demanded directions in the development of forensic examinations, and its results are quite reliable evidence of the involvement of a specific person in a crime. Due to its unique capabilities, molecular genetic analysis of DNA is a powerful tool in the investigation of criminal proceedings.

scholarly journals Forensic Molecular-Genetic Analysis of Objects of Biological Origin – a New Direction of Forensic Activity of the Russian Ministry of Justice

2021 ◽  
Vol 16 (1) ◽  
pp. 6-18
Author(s):  
S. A. Smirnova ◽  
G. G. Omel’yanyuk ◽  
I. V. Storozhenko ◽  
A. A. Rybakova ◽  
V. V. Gulevskaya
Keyword(s):  
Genetic Analysis ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Criminal Cases ◽  
Biological Origin ◽  
Forensic Dna Analysis ◽  
Forensic Institutions ◽  
History Of ◽  
Plant Biomaterials

The article addresses the importance and basic preconditions for the forming a new direction of forensic activity in the system of forensic institutions of the Russian Ministry of Justice a new direction of forensic activity - molecular-genetic analysis of the objects of biological origin. The authors present the advantages of DNA analysis - one of the most modern and efficient methods in investigating criminal cases. The article also demonstrates the potential of different methods of DNA-analysis for forensic investigations. The history of forensic DNA-analysis development in Russia and its features when examining the human, animal, and plant biomaterials are briefly discussed. The authors propose the definitions for the molecular-genetic examinations’ object and subject, formulate the model tasks, and suggest a list of sample questions for this study.

scholarly journals EVOLUTION OF DNA SEQUENCING METHODS AND PROSPECTS FOR THEIR USE IN FORENSIC DNA EXAMINATION

2020 ◽  
Vol 11 (87) ◽  
Author(s):  
Zhanna Bazyliuk ◽  
Keyword(s):  
Dna Sequencing ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Snp Markers ◽  
Degraded Dna ◽  
Phenotypic Traits ◽  
Nucleotide Polymorphisms ◽  
Biological Origin ◽  
Genetic Traits ◽  
Str Loci

The study of the human genome makes it possible to use genetic information to identify individual traits, diagnosis of diseases and forecasting and prevention of their development, promotes a personal approach when choosing treatment methods; population research, ethnogenesis and evolutionary processes. Introduction of DNA sequencing methods in domestic genetic fingerprinting will contribute to a more informative establishment of human genetic traits. The main purpose of molecular genetic research is to establish the genetic features of missing people, their relatives, to conduct paternity, to identify traces of biological origin and their identification. This article talks about the gradual development of DNA sequencing technology, which is conventionally divided into three types. The first type includes sequencing using capillary electrophoresis and pyrosequencing. The second type is high-throughput pyrosequencing, semiconductor, cyclic ligase, and the use of fluorescently labeled precursors, based on the sequencing of millions of DNA fragments simultaneously. The third stage includes methods that do not require prior sample preparation. These are methods of nanoporous sequencing, sequencing of one molecule, one-molecular sequencing. Today, each of the sequencing methods is aimed at performing different tasks. A number of methods are promising in the field of molecular-genetic examination. In world jurisprudence, sequencing is implemented mainly with the help of devices - Illumina’s, MiSeq FGx, Ion Torrent PGM from ThermoFisher and Ion S5. Research in forensic expertise of single nucleotide polymorphisms (SNP), sequencing of STR-loci and mitochondrial DNA, STR-loci and SNP-markers of the Y chromosome, will provide a high level of information, determination of human phenotypic traits, the possibility of establishing genetic traits from significantly degraded DNA. This article deals with modern problems of identification of human genetic traits and the prospect of introduction of the newest methods of sequencing for their qualitative and complete establishment.

The genetic analysis of growth and body composition in the mouse as a livestock model

2005 ◽  
Vol 2005 ◽  
pp. 239-239
Author(s):  
W. G. Hill ◽  
L. Bünger
Keyword(s):  
Body Composition ◽  
Food Intake ◽  
Genetic Analysis ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Molecular Genetic Analysis ◽  
Genetic Changes ◽  
Livestock Breeding ◽  
Farm Livestock ◽  
Human Genetic Research

The similarities between the mouse and farm livestock at the genetic and functional level make it a useful model for farm livestock breeding and indeed for human genetic research, taking the opportunities presented by its short generation interval, the development of inbred lines, and the ability to do gene knock-outs. Genetic similarities apply both at the molecular level, in gene structure and sequence, and at the quantitative level, for example genetic parameters of growth. We discuss here what can be learnt about the action and interaction of genes that influence traits of growth and body composition and contribute to genetic changes in them from work on the mouse, concentrating particularly on candidate genes and experiments conducted in our laboratory. As a resource we have developed highly divergent selection lines for growth, body composition and food intake, brought together lines of mice selected for growth from around the world, and inbred them both for stability and to facilitate their use for molecular genetic analysis. These lines differ greatly as a consequence of selection not only in growth and degree of fatness, but also in efficiency, as assessed by food intake corrected for body weight.

DNA-analysis of genotypes of the genus Fragaria l. For anthracnose resistance

2020 ◽  
Vol 62 ◽  
pp. 53-58
Author(s):  
A. S. Lyzhin ◽  
I. V. Luk’yanchuk ◽  
E. V. Zhbanova
Keyword(s):  
Genetic Analysis ◽  
Resistance Gene ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Anthracnose Resistance

The results of the molecular genetic analysis of strawberry genotypes for the Rca2 anthracnose resistance gene were shown. The marker STS-Rca2_240 linked to Rca2 gene was identified in the strawberry varieties Elianny and Laetitia (the putative genotype is Rca2Rca2 or Rca2rca2). In the remaining studied genotypes of the genus Fragaria L. the marker STS-Rca2_240 was not detected (the putative genotype is rca2rca2).

scholarly journals The Legal Policy of the Kyrgyz Republic on the Digitalization of Law Enforcement Agencies, Local Government Bodies at the Present Stage

2020 ◽  
Vol 6 (8) ◽  
pp. 216-221
Author(s):  
A. Beishembek kyzy ◽  
M. Abzhaparov
Keyword(s):  
Public Policy ◽  
Law Enforcement ◽  
Positive Impact ◽  
Human Life ◽  
Digital Technologies ◽  
Digital Transformation ◽  
Kyrgyz Republic ◽  
Present Stage ◽  
Law Enforcement Agencies

The article discusses government policy in the field of digital transformation of the country. The concept of public policy and digitalization is considered. It is noted that a properly constructed legal and state policy is the key to achieving success in the intended goal in the development of the country. An attempt is made to highlight the positive aspects of both competent public policy and the development of the state at the rate of using innovative technologies. The author tried to highlight the positive impact of the transition to digital technologies on the quality of human life and government activities in the future. Several examples of the implementation of automated information systems are given.

scholarly journals Monitoring of rabies in wild animals in the Kirov region after oral immunization

2016 ◽  
Vol 61 (4) ◽  
pp. 186-192 ◽  
Author(s):  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
A. L. Elakov ◽  
K. S. Kochergin-Nikitsky ◽  
T. I. Aliper ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Vaccine Strain ◽  
Oral Vaccine ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Oral Immunization ◽  
Molecular Genetic Analysis ◽  
Wild Isolate ◽  
Field Isolates ◽  
Pcr Techniques

This work presents the results of the molecular genetic research on genomes of field isolates of the rabies virus circulating in the territory of the Kirov region in order to analyze the phylogenetic relationship between the wild isolate genomes and to determine the possible reversion of the vaccine strain of the rabies virus used in the oral vaccine to virulent variant. We studied 24 brain samples from wild carnivores shot after oral immunization of the area with Rabivak-O/333. A bait with the vaccine provided by the Veterinary Service of the Kirov was also studied. All samples were found to be positive for the presence of the rabies virus as established by FAT and RT-PCR techniques. Phylogenetic analysis of N genome fragments of the rabies virus showed that the field isolates from the Kirov regions were genetically close to the field isolates from Buryatia 2012. Analysis of G genome fragments showed that the Kirov field isolates were close to the isolates from Lipetsk (2011), as well as to the Ukrainian isolates (2006 and 2010). Molecular genetic analysis of the gene fragments N and G for the field isolates and fragments of the genome of the rabies virus vaccine did not reveal any reversion to the virulent vaccine strain.

scholarly journals Forensic support of the activities of forensic science institutions and pre-trial investigation and inquiry bodies in counteraction to crime

2021 ◽  
Vol 23 (1) ◽  
pp. 61-74
Author(s):  
O. Yukhno
Keyword(s):  
Law Enforcement ◽  
Forensic Science ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Legal Framework ◽  
Modern State ◽  
Law Enforcement Agencies ◽  
Scientific Paradigm ◽  
Criminal Offenses ◽  
Forensic Expert

 Pressing issues of forensic support of activities of pre-trial investigation and inquiry bodies in countering criminal offenses are outlined. The concept and essence of crime counteraction are considered. The article analyzes the national criminal procedural legislation, departmental regulatory legal framework and their amendments concerning the direction under study, genesis of scientific findings on theoretical and applied issues of forensic support of the activities of pre-trial investigation and inquiry bodies in this direction, as well as the genesis of the concept and essence of forensic science as a science. The modern state of the development of forensic science, current high-priority issues and feasibility of changing the scientific paradigm of forensic science as well as the use of innovations are studied; ways for their improvement are proposed. Theoretical and applied problematic issues of criminalistics are subject to thorough study and resolution. Fundamental changes are required both in criminalistics in general and in particular in its individual areas. The current legislation, law enforcement agencies, forensic science institutions, prosecutors’ bodies and judicial bodies, as well as law enforcement, in which modern advances in science and technology (in particular, computer and telecommunication technologies) are being implemented should be reformed. The article highlights such problematic issues as the improvement of the forensic characteristics of cer-tain types of crimes, forensic techniques combining forensic techniques and tac-tics. The issue of further implementation in law enforcement and forensic expert activities of promising molecular genetic examinations for pre-trial investigation bodies, including the method of DNA analysis is outlined separately and fully. Relying on the results of research, specific author proposals and recommenda-tions are provided on the studied area of activity in general and in individual directions.

scholarly journals Modern principles and methods of objects of biological origin in forensic immunology

2017 ◽  
pp. 76-79
Author(s):  
Mykola Shevchuk ◽  
Iuliia Demchuk
Keyword(s):  
Research Methods ◽  
Biological Material ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Biological Origin ◽  
Forensic Expert ◽  
Material Evidence ◽  
Over Time

Over time, the more important question is the preservation of biological material discovered in the wake of material evidence during the immunological expertise for the further application of molecular genetic research methods, which in turn increases the objectivity of evidence and forensic expert results. The article suggested directions, the steps and methods of modern immunology expertise.

scholarly journals USE OF SEQUENCING METHODS FOR SPECIES IDENTIFICATION EXEMPLIFIED BY PHYLOGENETIC RELATIONSHIPS WITHIN GENUS HEDYSARUM L.

2021 ◽  
Vol 9 (6) ◽  
pp. 506-518
Author(s):  
D. R. Imachueva ◽  
F. K. Serebryanaya ◽  
E. M. Machs ◽  
V. V. Kotseruba
Keyword(s):  
Research Methods ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Raw Material ◽  
North Caucasus ◽  
Plant Materials ◽  
The North ◽  
Material Sources ◽  
5.8S Rrna

At the moment, a relevant objective in pharmacognosy, is the use of all kinds of the DNA analysis methods for identifying plant materials, detecting counterfeits, genetically modified crops and products.The aim of the research is to study the possibility of using molecular genetic research methods in the analysis of the genus Hedysarum L., for the identification of medicinal plant materials. This article presents the results of the application of molecular genetic research methods in the analysis of the genus Hedysarum L. in the flora of the North Caucasus.Materials and methods. The study material was the samples of the genus Hedysarum L. species collected in the North Caucasus: Hedysarum caucasicum M. Bieb. (in the fruiting phase in the territory of the Karachay-Cherkess Republic); Hedysarum grandiflorum Pall. (in the fruiting phase in the Volgograd region); Hedysarum daghestanicum Rupr. ex Boiss. (in the flowering phase in the Republic of Dagestan).  Sequencing of the ITS1-5.8S-ITS2 marker region of gene 5.8S by the RNA ribosome was carried out according to the Sanger method on the AbiPrism 3130 genetic analyzer at the laboratory of biosystematics and cytology of Komarov Botanical Institute of the Russian Academy of Sciences.Results. Based on a comparative study of the marker region of the nuclear ribosomal gene 5.8S rRNA, marker nucleotide substitutions of Hedysarum caucasicum M. Bieb., Hedysarum daghestanicum Rupr. ex Boiss., Hedysarum grandiflorum Pall, have been identified. The most probable secondary structure of 5.8S rRNA has been constructed. It has been shown that based on the analysis performed, it is possible to predict additional raw material sources of mangiferin and other groups of xanthones using the molecular data exemplified by the Obscura section.Conclusion. Based on the data obtained, it can be concluded that the morphological classification of the genus Hedysarum L. can be confirmed within the Obscura section.

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