scholarly journals USE OF SEQUENCING METHODS FOR SPECIES IDENTIFICATION EXEMPLIFIED BY PHYLOGENETIC RELATIONSHIPS WITHIN GENUS HEDYSARUM L.

2021 ◽  
Vol 9 (6) ◽  
pp. 506-518
Author(s):  
D. R. Imachueva ◽  
F. K. Serebryanaya ◽  
E. M. Machs ◽  
V. V. Kotseruba
Keyword(s):  
Research Methods ◽  
Dna Analysis ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Raw Material ◽  
North Caucasus ◽  
Plant Materials ◽  
The North ◽  
Material Sources ◽  
5.8S Rrna

At the moment, a relevant objective in pharmacognosy, is the use of all kinds of the DNA analysis methods for identifying plant materials, detecting counterfeits, genetically modified crops and products.The aim of the research is to study the possibility of using molecular genetic research methods in the analysis of the genus Hedysarum L., for the identification of medicinal plant materials. This article presents the results of the application of molecular genetic research methods in the analysis of the genus Hedysarum L. in the flora of the North Caucasus.Materials and methods. The study material was the samples of the genus Hedysarum L. species collected in the North Caucasus: Hedysarum caucasicum M. Bieb. (in the fruiting phase in the territory of the Karachay-Cherkess Republic); Hedysarum grandiflorum Pall. (in the fruiting phase in the Volgograd region); Hedysarum daghestanicum Rupr. ex Boiss. (in the flowering phase in the Republic of Dagestan).  Sequencing of the ITS1-5.8S-ITS2 marker region of gene 5.8S by the RNA ribosome was carried out according to the Sanger method on the AbiPrism 3130 genetic analyzer at the laboratory of biosystematics and cytology of Komarov Botanical Institute of the Russian Academy of Sciences.Results. Based on a comparative study of the marker region of the nuclear ribosomal gene 5.8S rRNA, marker nucleotide substitutions of Hedysarum caucasicum M. Bieb., Hedysarum daghestanicum Rupr. ex Boiss., Hedysarum grandiflorum Pall, have been identified. The most probable secondary structure of 5.8S rRNA has been constructed. It has been shown that based on the analysis performed, it is possible to predict additional raw material sources of mangiferin and other groups of xanthones using the molecular data exemplified by the Obscura section.Conclusion. Based on the data obtained, it can be concluded that the morphological classification of the genus Hedysarum L. can be confirmed within the Obscura section.

scholarly journals Phenolic Compounds and Antioxidant Potential of Wild-Growing Plant Materials of the North Caucasus Region

2019 ◽  
Vol 9 (2) ◽  
pp. 5062-5071 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Raw Materials ◽  
Functional Materials ◽  
Total Content ◽  
Raw Material ◽  
North Caucasus ◽  
Plant Materials ◽  
The North ◽  
Quantitative Content

The article presents the results of studying the qualitative composition and quantitative content of some groups of phenolic compounds in 11 types of medicinal plants growing in the foothills of the North Caucasus, and provides the estimates of the antioxidant activity of extracts from these plants. The qualitative and quantitative content of phenolic compounds was determined using a Kapel-105M capillary electrophoresis system, and the total antioxidant activity of the extracts was measured on a Tsvet Yauza-01-AA device with an amperometric detector. In the studied plant samples, the total content of tannins was determined, eight phenolcarbonic acids were identified and quantified, as well as quercetin and rutin — two of the most important flavonols. The highest total content of phenolcarbonic acids (11,776.2 mg/kg), as well as the highest antioxidant activity were noted in the aqueous extract obtained from Echinacea purpurea (lat. Echinacea angustifolia). The direct relationship between the antioxidant activity of the studied medicinal raw material and the content of phenolic compounds has been experimentally established as follows: the higher is the concentration of phenolic substances, the higher is the antioxidant activity. The results of this study provide new information on the composition and content of phenolic compounds in some types of wild-growing plant raw materials of the North Caucasus and the antioxidant activity of extracts based thereon that will facilitate the use of the studied plants as a potential source of natural antioxidants in the production of functional materials.

scholarly journals Genetic identification of bovine leukaemia virus

2018 ◽  
Vol 6 (2) ◽  
pp. 314-324 ◽  
Author(s):  
Irina Donnik ◽  
Irina Donnik ◽  
Ramil Vafin ◽  
Ramil Vafin ◽  
Aram Galstyan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Research Methods ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Genetic Identification ◽  
Gene Fragment ◽  
Phylogenetic Classification ◽  
Pcr Rflp

Molecular genetic research methods make it possible to evaluate the genetic diversity of bovine leukemia virus (BLV) and are the most informative approaches to its genetic identification. Molecular genetic research methods work well for the phylogenetic analysis of sequenced nucleotide DNA sequences of the provirus, as well as for the polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) according to the phylogenetic classification of the pathogen. The purpose of the research was to study the scientific and methodological approaches to the genetic identification of bovine leukemia virus, integrated into the molecular monitoring of infection of cattle with BLV genotypes. The authors used PCR-RFLP-genotyping and comparative phylogenetic analysis of aligned nucleotide sequences of the env gene fragment of the BLV provirus isolates to detect the genotypic affiliation of the cattle from twenty-one livestock farms of the Republic of Tatarstan. As a result, isolates of four out of ten BLV genotypes were found in the Tatarstani cattle, namely genotypes 1, 4, 7, and 8. The research involved a comparative analysis of 505 nucleotide sequences of a fragment of the BLV env gene, including those deposited in GenBank NCBI. The analysis confirms the inconsistency of several earlier PCR-RFLP typing strategies with the current approach in assessing the genotypic diversity by phylogenetic analysis. The improved strategy of PCR-RFLP genotyping of BLV corresponds with its modern phylogenetic classification. The strategy makes it possible to identify all the known genotypes of the viral pathogen. Its validity has been proved by in silico modelling of restrictogrammes and a phylogenetic analysis of the env gene fragment of 57 reference isolates of ten BLV genotypes that generate 57 genotype-associated combinations of diagnostically significant PCR-RFLP profiles.

scholarly journals TOPICALITY OF FORENSIC MOLECULAR GENETIC EXAMINATION AND ISSUES REGARDING ITS PERFORMING

2018 ◽  
Vol 18 ◽  
pp. 256-263
Author(s):  
V. V. Topchiy
Keyword(s):  
Dna Analysis ◽  
Human Life ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Molecular Genetic Analysis ◽  
Present Stage ◽  
Law Enforcement Agencies ◽  
Biological Origin ◽  
Genetic Features ◽  
Genetic Examination

Modern progress in forensic molecular genetic examination allow to obtain information about a particular person using traces variety of biological origin especially while committing grave crimes against human life and health, that are usually found at the scene and belong to a human body. A significant advantage of this method under crime investigation is precisely the safe exclusion of suspected persons not involved in the commission of a crime, in identifying those who committed a crime with a high probability level. At the present stage of forensic molecular genetic examination development there are significant gaps in legislation that are solved by adopting relevant normative and legal acts and improving existing ones. Effective method for of DNA analysis development is the creation of appropriate bases of genetic features of a person. However, the legislative consolidation of this process should take place in the context of respecting and protecting personal rights. However, terms of performing molecular genetic examination significantly exceed the terms of pre-trial investigation. This problem can be solved by expanding network of laboratories that perform such examination. Despite presence of a small number of problems, it is possible to affirm that DNA analysis is the most effective and reliable of all known methods of person identification at the present stage. At present, expert molecular genetic analysis develops not only as a section of molecular genetic research but also as a complete element of criminalistic knowledge that is aimed at investigating and disclosing crimes. Therefore, implementation of molecular genetic research methods into the practice of law enforcement agencies in Ukraine will significantly increase investigation effectiveness of many serious crimes against person.

scholarly journals Pottery of Early Iron Age from the Glinjeni II-La Șanț (North-Western Pontic Sea Region): Composition, Technology and Raw Material Sources

Heritage ◽  
2021 ◽  
Vol 4 (4) ◽  
pp. 2853-2875
Author(s):  
Marianna A. Kulkova ◽  
Maya T. Kashuba ◽  
Aleksandr M. Kulkov ◽  
Maria N. Vetrova
Keyword(s):  
Iron Age ◽  
Ceramic Technology ◽  
Raw Material ◽  
Early Iron Age ◽  
Cultural Traditions ◽  
The North ◽  
Material Sources ◽  
Cultural Layers ◽  
Thin Section Analysis ◽  
North Western

Transition to the Early Iron Age was marked by the appearance of innovations such as iron technology and changes in the lifestyle of local societies on the territory of the North-Western Pontic Sea region. One of the most interesting sites of this period is the Glinjeni II-La Șanț fortified settlement, located in the Middle Dniester basin (Republic of Moldova). Materials of different cultural traditions belonged to the Cozia-Saharna culture (10th–9th cc. BC) and the Basarabi-Șoldănești culture (8th–beginning of 7th cc. BC) were found on this site. The article presents the results of a multidisciplinary approach to the study of ceramic sherds from these archaeological complexes and cultural layers as well as raw clay sources from this area. The archaeometry analysis, such as the XRF-WD, the thin section analysis, SEM-EDX of ceramics, m-CT of pottery were carried out. The study of ancient pottery through a set of mineralogical and geochemical analytic methods allowed us to obtain new results about ceramic technology in different chronological periods, ceramic paste recipes and firing conditions. Correlation of archaeological and archaeometry data of ceramics from the Glinjeni II-La Șanț site gives us the possibility to differ earlier and later chronological markers in the paste recipes of pottery of 10th–beginning of 7th cc. BC in the region of the Middle Dniester basin.

scholarly journals Industri Alat Mikrolit di Situs Balang Metti: Teknologi Toala Akhir dan Kontak Budaya di Dataran Tinggi Sulawesi Selatan

AMERTA ◽  
2017 ◽  
Vol 35 (2) ◽  
pp. 93
Author(s):  
Suryatman Suryatman ◽  
Budianto Hakim ◽  
Afdalah Harris
Keyword(s):  
Early Modern ◽  
Research Methods ◽  
Late Pleistocene ◽  
Late Holocene ◽  
Raw Material ◽  
Cultural Contact ◽  
South Sulawesi ◽  
Material Sources ◽  
Early Modern Humans ◽  
Out Of Africa

Abstract. The Microlith Tool Industry at Balang Metti Site: Late Toalean Technology and Cultural Contact in the Highlands of South Sulawesi.The presence and distribution of microlith tools in Africa, Europe, and Asia have often been debated by prehistorians. The technology was brought by Early Modern Humans out of Africa to some areas of Europe and Asia during the Late Pleistocene. In South Sulawesi, it exists from the Middle to Late Holocene and is classed as part of the ‘Toalean’ culture. Excavations at Balang Metti site revealed a layer of microlith tools representing an industry that occurred for no more than 3,500 years ago. This is remarkable as the site is located in the highlands, whereas all previously known Toalean occupation sites are dispersed throughout the lowlands of South Sulawesi. The purpose of our research is to explain this microlith technology, especially the implication of its cultural contact, which occurred up to the highlands. Research methods done by classified, counted, and measured all lithic artefacts from excavation. The results show that the early stages of flaking (reduction) occurred not only in the cave but also out of the site, possibly close to the raw material sources. Abstrak.Kehadiran dan persebaran alat mikrolit di Afrika, Eropa, dan Asia telah diperdebatkan oleh kalangan peneliti prasejarah. Peralatan tersebut dibawa oleh manusia modern awal keluar dari Afrika ke beberapa wilayah Eropa dan Asia pada akhir Pleistosen. Di Sulawesi Selatan peralatan ini baru muncul pada pertengahan hingga akhir Holosen dan digolongkan sebagai bagian dari budaya Toalean. Penggalian di Situs Balang Metti menunjukkan lapisan budaya industri alat mikrolit berumur tidak lebih dari 3.500 tahun. Permasalahannya adalah situs tersebut berada di wilayah dataran tinggi, yang sebelumnya situs-situs hunian Toalean hanya ditemukan tersebar di wilayah dataran rendah Sulawesi Selatan. Penelitian ini bertujuan untuk menjelaskan teknologi alat mikrolit dan implikasi kontak budaya yang terjadi hingga di dataran tinggi Sulawesi Selatan. Metode penelitian dilakukan dengan mengklasifikasi, menghitung, dan mengukur semua artefak batu dari penggalian. Hasil penelitian menunjukkan bahwa proses penyerpihan tidak hanya dilakukan di dalam gua, tetapi juga di luar gua yang mungkin tidak jauh dari lokasi pengambilan bahan.

Behavioral and Molecular Genetics of Emotion Dysregulation

2018 ◽  
pp. 201-220
Author(s):  
Lance M. Rappaport ◽  
Sage E. Hawn ◽  
Cassie Overstreet ◽  
Ananda B. Amstadter
Keyword(s):  
Emotion Regulation ◽  
Psychiatric Disorders ◽  
Research Methods ◽  
Emotion Dysregulation ◽  
Molecular Genetic ◽  
Critical Role ◽  
Genetic Research ◽  
Emotional Dysregulation ◽  
Underlying Structure

Given the critical role that emotion dysregulation plays in many psychiatric disorders, there is a need to understand the biological underpinnings of emotion regulation deficits. This chapter opens with a brief overview of emotion regulation and constructs that fall under its broad umbrella. Next, it provides a brief primer of behavioral genetic research methods, summarizes existing literature regarding the heritability of emotional dysregulation, provides an overview of molecular genetic research methods, and reviews extant molecular genetic literature on emotion regulation. Finally, the chapter reviews the limitations of existing research and identifies promising areas of future inquiry that may clarify the underlying structure of emotion dysregulation and identify the role of common genetic loci in associations between emotion dysregulation and psychopathology.

scholarly journals The study of amino acid composition of roots of dandlion (Taraxacum officinale F.H. Wigg.) growing in the North Caucasus

2020 ◽  
Vol 20 (5-6) ◽  
pp. 184-190
Author(s):  
Anastasia O. Suschenko ◽  
Evgenia V. Kompantseva
Keyword(s):  
Amino Acids ◽  
Medicinal Plant ◽  
Human Body ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
North Caucasus ◽  
Isoleucine Leucine ◽  
The North ◽  
Plant Raw Material

Aim. Being an integral part of proteins, amino acids are involved in all physiological processes in the human body. Plants being an accessible source of biologically active substances, there is a need to study medicinal plant raw material containing amino acids that are interchangeable and essential for the human body. Materials and methods. Air-dry roots of dandelion (Тaraxacum officinale Wigg.) collected on the slopes of mount Mashuk (Pyatigorsk) in April and September 2018 were studied. They were cleared of traces of soil. To determine the bound forms of amino acids the raw material was prepared by hydrolysis during heating. Data about the composition and amount of amino acids in the analyzed samples of dandelion roots were obtained by means of the system of capillary electrophoresis Drops-105. Processing of the data was carried out with the use of the computer program Multichrom for Windows. Results and discussion. 13 free amino acids were found in the roots of dandelion, of which 7 were nonessential (-alanine, glycine, serine, tyrosine, glutamic acid, arginine, proline) and 6 were essential amino acids (valine, isoleucine, leucine, methionine, threonine, -phenylalanine). Bound amino acids were found to include 8 nonessential amino acids (-alanine, glycine, serine, tyrosine, arginine, Proline, glutamic and aspartic acid) and 6 essential ones (valine, isoleucine, leucine, methionine, threonine, -phenylalanine). The amount of free and bound amino acids in the roots of dandelion collected in April is about 1.4% by weight of the raw material and reaches 3.5% in the roots collected in September. Conclusion. The qualitative and quantitative composition of amino acids in dandelion roots growing in the North Caucasus has been studied. The detected amino acids make a certain contribution to the total pharmacological effect of this type of medicinal plant raw material.

scholarly journals Complex experimental-trasologic and ethnographic researches of ceramics: technology of manufacturing and burning

2018 ◽  
Vol 7 (3) ◽  
pp. 165-172
Author(s):  
Galina Nikolaevna Poplevko
Keyword(s):  
Research Methods ◽  
Experimental Modeling ◽  
North Caucasus ◽  
Ancient Ceramics ◽  
The North ◽  
Ethnographic Studies ◽  
Series Of Experiments ◽  
Ethnographic Data ◽  
First Time ◽  
Technological Methods

Complex experimental-trasological and ethnographic studies of ceramics are shown on the example of their application to the study of technological methods for the manufacture of ancient ceramics. The paper considers the use of different research methods using the materials of Rakushechny Yar settlement on the Lower Don and Maikop culture in the North Caucasus and ethnographic data. Experimental modeling of flat-bottomed vessels of the Neolithic and round-bottomed vessels of Maikop culture is given as an example. It is shown that ceramics in different archaeological epochs were made using several techniques. The technique of making vessels was modeled by experiments. The study of ethnographic data on the technique of making round-bottomed vessels shows that the methods of modeling different vessels are much larger. The work presents archaeological, experimental, tracological and ethnographic data on the production of both flat-bottomed and round-bottomed vessels using the knockout technique. Recently, with the help of this technique, a series of experiments on the modeling of vessels and the subsequent tracological study of the technology of their manufacture with the help of a knockout was performed. For the first time the author shows the use of a combination of various methods of modeling in the manufacture of Neolithic vessels. The data of petrography and experiments on the burning of vessels are given.

scholarly journals Orchidaceae Juss. in Flora of Chechnya and Their Protection

2019 ◽  
Author(s):  
E L Tasueva ◽  
R S Magomadova ◽  
A S Abdurzakova ◽  
A M Umaeva ◽  
Z I Shakhgirieva ◽  
...  
Keyword(s):  
Vegetation Cover ◽  
Family Members ◽  
Common Species ◽  
Raw Material ◽  
North Caucasus ◽  
The North ◽  
The Family ◽  
Protected Plants ◽  
First Time

For the first time, perennial materials on the Orchidacaeae Juss. family of Chechnya are summarizedб consisting of 15 families and 29 species. The largest genus in the family – Orchis L. is represented by 8 species. The article presents information that all members of the family are protected plants listed in the Red Book of Chechnya, Ingushetia, Dagestan, and the Stavropol Territory. Some species are not included in the list of plants to be protected, including those species that have underground tuberoids and can serve as a source of salep (for instance, Platanthera bifolia, P. chlorantha, Herminium monorchis, Traunsteinera sphaerica, Dactylorhisa incarnata, D. salina, D. urvilleana). In this connection, data on the resources of family members and the potential possibilities of their use as a medicinal raw material are of interest. We have carried out resource studies of the most common species of Orchis tridentata Scop, within the North Caucasus. The obtained data allow us to expand our understanding of the species role of Orchidaceae Juss family. Пoлyчeнныe дaнныe пoзBoляют pacшиpить пpeдcтaBлeниe o poли BидoB ceмeйcтBa Orchidaceae Juss. of the North Caucasus flora in the composition of vegetation cover.

scholarly journals Modern principles and methods of objects of biological origin in forensic immunology

2017 ◽  
pp. 76-79
Author(s):  
Mykola Shevchuk ◽  
Iuliia Demchuk
Keyword(s):  
Research Methods ◽  
Biological Material ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Biological Origin ◽  
Forensic Expert ◽  
Material Evidence ◽  
Over Time

Over time, the more important question is the preservation of biological material discovered in the wake of material evidence during the immunological expertise for the further application of molecular genetic research methods, which in turn increases the objectivity of evidence and forensic expert results. The article suggested directions, the steps and methods of modern immunology expertise.

Sign in / Sign up

Export Citation Format

Share Document