scholarly journals Oral carriage of methicillin-resistant Staphylococcus aureus strains among patients with diabetic foot ulcer

2020 ◽  
pp. 5-12
Keyword(s):  
Staphylococcus Aureus ◽  
Oral Cavity ◽  
Diabetic Foot ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Diabetic Patients ◽  
Methicillin Resistant ◽  
Mrsa Strains

Introduction: Diabetic foot ulcer (DFU) caused by Staphylococcus aureus is one of the most feared complications of diabetes mellitus. The studies reporting the oral cavity as a potential reservoir of S. aureus in diabetic patients are sparse. The aim of the study was to compare the prevalence of methicillin-resistant Staphylococcus aureus strains in the oral and in the diabetic foot specimens from DFU patients. Materials and Methods: A total 80 specimens (40 oral swabs and 40 DFU swabs) were collected from diabetic patients with foot ulcer. The specimens were subcultured and the susceptibility of isolated S. aureus strains to antimicrobial agents was determined. Suspected methicillin-resistant S. aureus (MRSA) strains were further examined for the presence of modified PBP2a protein. Results: Less than one-fifth of patients with DFU had oral S. aureus carriage, however the colonization is significantly associated with S. aureus diabetic foot infection. S. aureus strains were isolated from 52.5% of DFU specimens, 17.5% were resistant to methicillin. S. aureus strains were isolated from 17.5% of oral specimens of diabetic patients; 2.5% were methicillin-resistant. The MRSA strains were isolated sevenfold more frequently from the diabetic foot than from the oral cavity. Conclusions: Although diabetic foot infections caused by an endogenous S. aureus strains colonizing the oral cavity of diabetic patients seems unlikely, it is evidently important to monitor the oral S. aureus carriage in diabetic patients and their resistance to antibiotics.

Methicillin-Resistant Staphylococcus aureus Endocarditis from a Diabetic Foot Ulcer

2018 ◽  
Vol 108 (6) ◽  
pp. 528-531 ◽  
Author(s):  
Andrew J. Hale ◽  
Emily Vicks ◽  
Mary T. LaSalvia ◽  
John M. Giurini ◽  
Adolf W. Karchmer
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
The United States ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers ◽  
Methicillin Resistant ◽  
Diabetic Foot Infections

Diabetic foot infections are a common cause of morbidity and mortality in the United States, and successful treatment often requires an aggressive and prolonged approach. Recent work has elucidated the importance of appropriate therapy for a given severity of diabetic foot infection, and highlighted the ongoing risk such patients have for subsequent invasive life-threatening infection should diabetic foot ulcers fail to heal. The authors describe the case of a man with diabetes who had prolonged, delayed healing of a diabetic foot ulcer. The ulcer subsequently became infected by methicillin-resistant Staphylococcus aureus (MRSA). The infection was treated conservatively with oral therapy and minimal debridement. Several months later, he experienced MRSA bloodstream infection and complicating endocarditis. The case highlights the ongoing risk faced by patients when diabetic foot ulcers do not heal promptly, and emphasizes the need for aggressive therapy to promote rapid healing and eradication of MRSA.

scholarly journals Case report: Infected foot ulcer and methicillin-resistant staphylococcus aureus bacteremia in a diabetic patient

2019 ◽  
Vol 6 (2) ◽  
pp. 20
Author(s):  
Margarita Goula ◽  
Patricia Felix ◽  
Meta-Celestine Mbuyamba ◽  
Benoit Pirotte
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Patient ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Diagnostic Tools ◽  
Methicillin Resistant ◽  
Staphylococcus Aureus Bacteremia ◽  
Peripheral Arterial ◽  
The Right

Diabetic foot ulcer is one of the most frequent complications affecting the diabetic patient. While there has been considerable progress regarding the diagnostic tools and the range of antibiotic choices, the outcome is often unsatisfactory, the presence of peripheral arterial occlusive disease being among the main factors influencing the evolution. This paper describes the management of a patient presenting with an infected foot ulcer and persistent methicillin-resistant Staphylococcus aureus bacteremia, furthercomplicated by spondylodiskitis and infectious endocarditis: the case gives an opportunity to review the literature in search of theoptimal care and the right treatment choices.

scholarly journals Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 439 ◽  
Author(s):  
Vanessa Silva ◽  
Telma de Sousa ◽  
Paula Gómez ◽  
Carolina Sabença ◽  
Madalena Vieira-Pinto ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Spa Typing ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Resistance To Penicillin ◽  
Mrsa Strains

Methicillin-resistant Staphylococcus aureus (MRSA) are one of the main pathogens associated with purulent infections. MRSA clonal complex 97 (CC97) has been identified in a wide diversity of livestock animals. Therefore, we aimed to investigate the antibiotic resistance profiles of MRSA strains isolated from purulent lesions of food-producing rabbits. Samples from purulent lesions of 66 rabbits were collected in a slaughterhouse in Portugal. Samples were seeded onto ORSAB plates with 2 mg/L of oxacillin for MRSA isolation. Susceptibility to antibiotics was tested by the disk diffusion method against 14 antimicrobial agents. The presence of resistance genes, virulence factors and the immune evasion cluster (IEC) system was studied by polymerase chain reaction. All isolates were characterized by multilocus sequence typing (MLST), agr and spa typing. From the 66 samples analyzed, 16 (24.2%) MRSA were detected. All strains were classified as multidrug-resistant as they were resistant to at least three classes of antibiotics. All isolates showed resistance to penicillin, erythromycin and clindamycin. Seven isolates were resistant to gentamicin and harbored the aac(6′)-Ie-aph (2″)-Ia gene. Resistance to tetracycline was detected in 10 isolates harboring the tet(K) gene. The IEC genes were detected in three isolates. MRSA strains belonged to CC97, CC1, CC5, CC15 or CC22. The isolates were assigned to six different spa types. In this study we found a moderate prevalence of multidrug-resistant MRSA strains in food-producing rabbits. This may represent concern for food safety and public health, since cross-contamination may occur, leading to the spread of MRSA and, eventually, the possibility of ingestion of contaminated meat.

scholarly journals Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Foot Infection

2019 ◽  
Vol 14 (4) ◽  
pp. 329-337 ◽  
Author(s):  
Pegah Kananizadeh ◽  
Solmaz Ohadian Moghadam ◽  
Yasaman Sadeghi ◽  
Abbas Rahimi Foroushani ◽  
Hossein Adibi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Diffusion Method ◽  
Diabetic Patients ◽  
Test Technique ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Patients With Diabetes

Background & Objective: Diabetic foot ulcer (DFU), is one of the most frequent causes for hospitalizations in patients with diabetes. A major problem in the treatment of DFU is the increased-incidence of methicillin-resistant Staphylococcus aureus (MRSA). The aim of this study was to determine the SCCmec types of MRSA isolates and their epidemiology among patients with diabetes. Methods: This study was carried out on 145 diabetic patients with DFUs. The antibiotic susceptibility tests (ASTs) were performed using the disk diffusion method and E-test technique. SCCmec typing was done by multiplex PCR. Moreover, the presence of virulence toxin genes, including pvl and lukED was detected by PCR assay. Results: In 145 samples from which S. aureus was predominantly isolated, 19.48% were MRSA. Analysis of MRSA isolates revealed that the most prevalent SCCmec type was type IV (46.7%) followed by type III (30.0%) and type V (20.0%). One strain (3.3%) was untypeable. The prevalence of pvl and lukED was 56.7% and 100%, respectively. Conclusion: The high prevalence of MRSA in DFUs represents the high levels of antibiotic usage among patients with diabetes. In this study, resistance to other important clinical antibiotics was detected among MRSA isolates. The high proportion of SCCmec type IV and V strains, even in former hospitalized patients, indicates the entrance of these clones to the clinical setting.

scholarly journals Microbiological Study of Diabetic Foot Ulcer

2018 ◽  
Vol 8 (3) ◽  
pp. 251-256
Author(s):  
Mohammuddunnobi ◽  
Tasnuva Jahan ◽  
Abdullah Al Amin
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Klebsiella Pneumonia ◽  
Diabetic Patients ◽  
Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening infection in diabetic patients are usually polymicrobial involving both multiple aerobic and anaerobic organisms.Methods: The present study was a cross sectional study, conducted in the Department of Surgery and Microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September’ 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is ‘any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus’.Results: 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillinclavulanic acid, amikacin, piperacillin-tazobactam. In Coagulase-negative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem.Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Citrobacter spp.Birdem Med J 2018; 8(3): 251-256

scholarly journals Efficacy of vancomycin in combination with various antimicrobial agents against clinical methicillin resistant Staphylococcus aureus strains

2020 ◽  
Vol 37 (1) ◽  
Author(s):  
Gulseren Aktas
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Additive Interaction ◽  
Methicillin Resistant ◽  
Creative Commons ◽  
Mrsa Strains ◽  
New Generation ◽  
Open Access Article

Background: Multi-drug resistant methicillin resistant Staphylococcus aureus (MRSA) strains that have been isolated frequently worldwide have difficulties in the treatment and therefore alternative choices for the treatment of the infections are required. The aim of the study was to evaluate the interaction of various antimicrobials in combination with vancomycin against MRSA. Methods: Twenty five clinical MRSA strains isolated in 2016 were included in the study. The interaction between vancomycin and new generation/conventional antimicrobials against MRSA strains was analyzed by E-test. Results: All of the strains tested was found to be susceptible to vancomycin, telavancin, dalbavancin, ceptobiprole, daptomycin, linezolid, quinupristin-dalfopristin, trimethoprim-sulfamethoxazole, rifampicin and tigecycline. The susceptibility rates of the isolates were found to be high, with the lowest rate (48%) against azithromycin. According to the fractional inhibitory concentration index results, synergistic interaction with vancomycin was determined with trimethoprim-sulfamethoxazole, azithromycin, linezolid, minocycline, dalbavancin, clindamycin in five, three, two, two, one, one and one strain(s), respectively. Additionally, all combinations studied showed additive interaction at high rates. Conclusions: The results of the study indicate that the use of vancomycin in combination with conventional and new generation antibiotics is promising. doi: https://doi.org/10.12669/pjms.37.1.2887 How to cite this:Aktas G. Efficacy of vancomycin in combination with various antimicrobial agents against clinical methicillin resistant Staphylococcus aureus strains. Pak J Med Sci. 2021;37(1):151-156. doi: https://doi.org/10.12669/pjms.37.1.2887 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Thiazolidines; The Potential Antimicrobial Agents Against Methicillin-Resistant Strains of Staphylococcus aureus

2020 ◽  
Vol 1 (1) ◽  
pp. 9-15
Author(s):  
Mehwish Tanveer ◽  
Hira Musaddiq ◽  
Atia Iqbal ◽  
Sarwat Saleem ◽  
Sadia Iqbal ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Resistant Strains ◽  
Mrsa Strains

2-Substituted 1,3-Thiazolidine-4-Carboxylic Acids (1-11) were synthesized and screened for their anti-methicillin-resistant Staphylococcus aureus (MRSA) potential. The synthesized compounds were evaluated for their antibacterial activities against four MRSA strains MRSA I, VI, VII and VIII with accession numbers KU662352, KR862285, KR862291 and KU662354 respectively by well diffusion method. In addition, antibacterial evaluations were also performed for gram positive strain Bacillus subtilis and gram-negative strains klebsiella pneumoniae and pseudomonas aeruginosa using same method. Most of the synthesized thiazolidine-4-carboxylic acid derivatives exhibited better antibacterial activities against studied bacterial strains. Amongst the synthesized compounds, 8-10 were found to possess significant activity (Zone in mm) against methicillin-resistant Staphylococcus aureus in addition to the other studied bacterial strains.

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