scholarly journals DEVELOPMENT OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR QUANTITATION OF ?-SITOSTEROL IN NORMACID SYRUP: A POLY HERBAL FORMULATION

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Shah Kinjal H ◽  
Makwana Rajeshree
Keyword(s):  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Chromatography Method ◽  
Plant Parts ◽  
Herbal Formulation ◽  
Quality Control Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Nanogram Level

Normacid syrup consisiting of 17 ingredients, used in the treatment of hyperacidity and gastritis. ?- Sitosterol is one of the phytoconstituent present in Normacid syrup- a poly herbal formulation. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of ?- Sitosterol in dried plant parts used in formulations. This HPTLC method was found to be reproducible, accurate, precise and detect ?- Sitosterol at nanogram level. The developed HPTLC method would be an important tool in the quality control method of poly herbal formulation.  Keywords: HPTLC, Polyherbal, ?- Sitosterol, Gastritis

scholarly journals Development of High Performance Thin Layer Chromatography Method for Quantitation of Gallic Acid in Normacid Syrup: A Poly Herbal Formulation

2021 ◽  
Vol 11 (1-s) ◽  
pp. 49-52
Author(s):  
Kinjal H Shah ◽  
Rajeshree Makwana
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Chromatography Method ◽  
Herbal Formulation ◽  
Quality Control Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Nanogram Level

Normacid syrup consisiting of 17 ingredients, used in the treatment of hyperacidity and gastritis. Gallic acid is one of the phytoconstituent present in Normacid syrup- a poly herbal formulation. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of gallic acid in dried fruits used in formulations. This HPTLC method was found to be reproducible, accurate, precise and detect gallic acid at nanogram level. The developed HPTLC method would be an important tool in the quality control method of poly herbal formulation.  Keywords: HPTLC, Polyherbal, Gallic acid, Gastritis

scholarly journals In Vitro Propagation, Development and Validation of HPTLC Method for Quantification of Steroidal Glycoalkaloid Solasodine in In Vitro Culture and Plant Parts of Solanum Nigrum

2018 ◽  
Vol 34 (6) ◽  
pp. 2728-2734 ◽  
Author(s):  
Mohd Amir ◽  
Niyaz Ahmad ◽  
Mohammad Sarafroz ◽  
Mahvish Jamal ◽  
Faheem Hyder Pottoo ◽  
...  
Keyword(s):  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Statistical Investigation ◽  
Solanum Nigrum ◽  
Nodal Segment ◽  
Standard Drug ◽  
Plant Parts ◽  
Hptlc Method

Solanum nigrum Linn. (Family: solanaceae), has medicinal values, commonly exploited for its active constituents which are of high pharmaceutical importance. The plant contains different type of natural compounds, and one of most important glycoalkaloid in S. nigrum is solasodine. Nodal segment of S. nigrum was cultured on MS media added with several concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4- D) (0.5ppm –2ppm) for callus induction and development. For shoot induction, MS medium complemented with different dilutions of 6- benzyl adenine (6- BA) (0.5ppm–2ppm) was used. A novel, rapid, and simple high performance thin layer chromatographic method for quantitative estimation of triterpenoid glycoalkaloid. Solasodine was spotted on silica gel 60F254 TLC plate using chloroform: methanol (8.75: 1.25; v/v) as mobile phase. Anisaldehyde-sulfuric acid in methanol used as derivatizing reagent and quantified by densitometric study at 366 nm. This scheme was found to give a sharp and compact spot for the standard drug (Rf = 0.67 ± 0.04). The linearity of this method was found to be a range of 100-1000 ng per spot with LOD and LOQ of 23.78 ng per spot and 67.09 ng per spot; respectively. This method has shown recoveries of solasodine in the range from 98.23% -100.88 %, with low intra and inter-day % RSD. Statistical investigation of the data presented that the method is selective, accurate, precise and reproducible for the study of solasodine. The established and developed HPTLC method would be a significant parameter for the analysis of quality control method of herbal drugs and formulations.

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

scholarly journals HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF β-SITOSTEROL FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

2020 ◽  
pp. 162-165
Author(s):  
RIDHI JOSHI ◽  
RISHIKESH MEENA ◽  
PREETI MISHRA ◽  
VIDYA PATNI
Keyword(s):  
High Performance ◽  
Normal Phase ◽  
Leaf Sample ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

scholarly journals IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

2017 ◽  
Vol 9 (9) ◽  
pp. 126
Author(s):  
Pratheema Philomindoss
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
Chromatography Method ◽  
Ich Guidelines ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

Stability Indicating Simultaneous Estimation of Cetirizine Hydrochloride and Phenylephrine Hydrochloride in Combined tablet formulation by using HPTLC- Densitometric method

2021 ◽  
pp. 2467-2471
Author(s):  
M.R. Bodhankar ◽  
S.J. Wadher ◽  
T.M. Kalyankar ◽  
Anitha K
Keyword(s):  
High Performance ◽  
Heat Exposure ◽  
Calibration Plot ◽  
Simultaneous Estimation ◽  
Chromatography Method ◽  
Phenylephrine Hydrochloride ◽  
Solid Dosage ◽  
Cetirizine Hydrochloride ◽  
Layer Chromatography ◽  
Hptlc Method

The new simple, precise, rapid, selective, high performance thin layer chromatography method has been developed for simultaneous estimation of cetirizine hydrochloride and phenylephrine hydrochloride in its combined solid dosage form. The HPTLC method was performed on precoated silica gel G60 F254 plates with, Toluene: Methanol: Ammonia (8: 2: 1, v/v/v) as optimum mobile phase. The plates were developed in 6.00× 0.30mm at room temperature. The developed plates were scanned and quantified at their absorption at 224 nm for CET and PHE respectively. The drugs were satisfactorily resolved with Rf = 0.36± 0.001 for CET and 0.65±0.004 for PHE. Calibration plot was linear between 100-600 ng/ spot for CET and 200-1200 ng/ spot for PHE. This HPTLC method is sensitive, economical, less time consuming than other chromatographic procedures. It can become important tool for analysis of combined solid dosage from. In addition to this results acid exposure, base exposure, oxidative exposure, neutral exposure, photolysis and dry heat exposure is carried out.

scholarly journals DENSITOMETRIC VALIDATION OF LAPACHOL IN TECOMELLA UNDULATA SEEM BARK BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2018 ◽  
Vol 10 (7) ◽  
pp. 87
Author(s):  
Raj Richa ◽  
Siddiqui Nahida ◽  
Aeri Vidhu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Quantitative Estimation ◽  
Marker Compound ◽  
Soxhlet Apparatus ◽  
Peak Areas ◽  
Tecomella Undulata ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: To develop a validated high-performance thin layer chromatography (HPTLC) method for the quantitative estimation of Lapachol in a Soxhlet extracted bark of Tecomella undulata seem (T. undulata). Methods: The bark of T. undulata was extracted with chloroform by Soxhlet apparatus. The separation was achieved on a silica-gel 60 F254 HPTLC plate using toluene-ethyl acetate–glacial acetic acid (8.5:1.5:0.02 v/v/v) as a mobile phase. Densitometric analysis of Lapachol was carried out in absorbance mode at 254 nm.Results: The proposed method was accurate for the separation and resolution between peaks of the standard and Lapachol (5.04µg) with Rf value 0.77. Calibration curves were found to be linear over the concentration range (10-130µg) for Lapachol and correlation coefficient over (R2= 0.9973), indicating an excellent correlation between peak areas and concentrations of the marker compound. The experimentally derived LOD and LOQ for Lapachol were determined to be 0.028 µg and 0.086 µg respectively and the developed HPTLC-UV method showed lower %RSD and SEM, value indicating the method to be precise, accurate and robust.Conclusion: The study concludes that HPTLC-UV validation method can be very efficient and promising technique for the identification and quantitative analysis of Lapachol from T. undulata bark. The statistical analysis of data indicates that the developed method is reproducible and specific.

scholarly journals Embelin: an HPTLC method for quantitative estimation in five species of genus Embelia Burm. f.

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
K. P. Rini Vijayan ◽  
A. V. Raghu
Keyword(s):  
High Performance ◽  
Quantitative Estimation ◽  
Biological Activities ◽  
Bioactive Molecules ◽  
Plant Parts ◽  
Embelia Ribes ◽  
Ich Guidelines ◽  
Medicinal Properties ◽  
Taxonomic Studies ◽  
Hptlc Method

Abstract Background The plants belonging to the genus Embelia, a significant tropical genus with many biological activities, are benefiting because of their robust medicinal properties. Embelin is one of the principal bioactive molecules responsible for the medicinal properties of the genus Embelia. The quantification of the embelin compound among different species in this genus has not yet been investigated, so still uncertain which species and which part should be accepted. The present study was intended to establish a speedy and precise high-performance thin-layer chromatographic (HPTLC) method for quantitative study of embelin in various plant parts of Embelia ribes, Embelia tsjeriam-cottam, Embelia basaal, Embelia adnata, and Embelia gardneriana. Result This research confirmed the method as per the International Conference on Harmonization (ICH) guidelines. We achieved separation on silica gel 60 F254 HPTLC plates using propanol: butanol: ammonia (7:3:7 v/v/v) as a mobile phase. Densitometry scanning performed for detection and quantification at 254 nm and 366 nm. Among the species investigated, the highest amount of embelin was found in E. ribes fruits. Conclusion Embelia ribes fruits are the best source of embelin. Embelin was first described in the endemic species, such as E. adnata and E. gardneriana. The method illustrated in this research may be applied for quantification of embelin and fingerprint analysis of other species within Embelia genus or described genera and chemo taxonomic studies of this genus.

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