scholarly journals Development of High Performance Thin Layer Chromatography Method for Quantitation of Gallic Acid in Normacid Syrup: A Poly Herbal Formulation

2021 ◽  
Vol 11 (1-s) ◽  
pp. 49-52
Author(s):  
Kinjal H Shah ◽  
Rajeshree Makwana
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Chromatography Method ◽  
Herbal Formulation ◽  
Quality Control Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Nanogram Level

Normacid syrup consisiting of 17 ingredients, used in the treatment of hyperacidity and gastritis. Gallic acid is one of the phytoconstituent present in Normacid syrup- a poly herbal formulation. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of gallic acid in dried fruits used in formulations. This HPTLC method was found to be reproducible, accurate, precise and detect gallic acid at nanogram level. The developed HPTLC method would be an important tool in the quality control method of poly herbal formulation.  Keywords: HPTLC, Polyherbal, Gallic acid, Gastritis

scholarly journals DEVELOPMENT OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR QUANTITATION OF ?-SITOSTEROL IN NORMACID SYRUP: A POLY HERBAL FORMULATION

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Shah Kinjal H ◽  
Makwana Rajeshree
Keyword(s):  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Chromatography Method ◽  
Plant Parts ◽  
Herbal Formulation ◽  
Quality Control Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Nanogram Level

Normacid syrup consisiting of 17 ingredients, used in the treatment of hyperacidity and gastritis. ?- Sitosterol is one of the phytoconstituent present in Normacid syrup- a poly herbal formulation. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of ?- Sitosterol in dried plant parts used in formulations. This HPTLC method was found to be reproducible, accurate, precise and detect ?- Sitosterol at nanogram level. The developed HPTLC method would be an important tool in the quality control method of poly herbal formulation.  Keywords: HPTLC, Polyherbal, ?- Sitosterol, Gastritis

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

scholarly journals IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

2017 ◽  
Vol 9 (9) ◽  
pp. 126
Author(s):  
Pratheema Philomindoss
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
Chromatography Method ◽  
Ich Guidelines ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

Stability Indicating Simultaneous Estimation of Cetirizine Hydrochloride and Phenylephrine Hydrochloride in Combined tablet formulation by using HPTLC- Densitometric method

2021 ◽  
pp. 2467-2471
Author(s):  
M.R. Bodhankar ◽  
S.J. Wadher ◽  
T.M. Kalyankar ◽  
Anitha K
Keyword(s):  
High Performance ◽  
Heat Exposure ◽  
Calibration Plot ◽  
Simultaneous Estimation ◽  
Chromatography Method ◽  
Phenylephrine Hydrochloride ◽  
Solid Dosage ◽  
Cetirizine Hydrochloride ◽  
Layer Chromatography ◽  
Hptlc Method

The new simple, precise, rapid, selective, high performance thin layer chromatography method has been developed for simultaneous estimation of cetirizine hydrochloride and phenylephrine hydrochloride in its combined solid dosage form. The HPTLC method was performed on precoated silica gel G60 F254 plates with, Toluene: Methanol: Ammonia (8: 2: 1, v/v/v) as optimum mobile phase. The plates were developed in 6.00× 0.30mm at room temperature. The developed plates were scanned and quantified at their absorption at 224 nm for CET and PHE respectively. The drugs were satisfactorily resolved with Rf = 0.36± 0.001 for CET and 0.65±0.004 for PHE. Calibration plot was linear between 100-600 ng/ spot for CET and 200-1200 ng/ spot for PHE. This HPTLC method is sensitive, economical, less time consuming than other chromatographic procedures. It can become important tool for analysis of combined solid dosage from. In addition to this results acid exposure, base exposure, oxidative exposure, neutral exposure, photolysis and dry heat exposure is carried out.

scholarly journals DENSITOMETRIC VALIDATION OF LAPACHOL IN TECOMELLA UNDULATA SEEM BARK BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2018 ◽  
Vol 10 (7) ◽  
pp. 87
Author(s):  
Raj Richa ◽  
Siddiqui Nahida ◽  
Aeri Vidhu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Quantitative Estimation ◽  
Marker Compound ◽  
Soxhlet Apparatus ◽  
Peak Areas ◽  
Tecomella Undulata ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: To develop a validated high-performance thin layer chromatography (HPTLC) method for the quantitative estimation of Lapachol in a Soxhlet extracted bark of Tecomella undulata seem (T. undulata). Methods: The bark of T. undulata was extracted with chloroform by Soxhlet apparatus. The separation was achieved on a silica-gel 60 F254 HPTLC plate using toluene-ethyl acetate–glacial acetic acid (8.5:1.5:0.02 v/v/v) as a mobile phase. Densitometric analysis of Lapachol was carried out in absorbance mode at 254 nm.Results: The proposed method was accurate for the separation and resolution between peaks of the standard and Lapachol (5.04µg) with Rf value 0.77. Calibration curves were found to be linear over the concentration range (10-130µg) for Lapachol and correlation coefficient over (R2= 0.9973), indicating an excellent correlation between peak areas and concentrations of the marker compound. The experimentally derived LOD and LOQ for Lapachol were determined to be 0.028 µg and 0.086 µg respectively and the developed HPTLC-UV method showed lower %RSD and SEM, value indicating the method to be precise, accurate and robust.Conclusion: The study concludes that HPTLC-UV validation method can be very efficient and promising technique for the identification and quantitative analysis of Lapachol from T. undulata bark. The statistical analysis of data indicates that the developed method is reproducible and specific.

scholarly journals A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation

2019 ◽  
Vol 7 (1) ◽  
pp. 7-13
Author(s):  
Vijaykumar K. Parmar ◽  
Deepika Mohanta ◽  
Harsh Shah
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Simultaneous Estimation ◽  
Berberine Chloride ◽  
Herbal Formulation ◽  
Ich Guidelines ◽  
System Suitability ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68 ± 0.02, respectively. The response in terms of peak area was found to be linear over the concentration range of 100-500 ng/spot for berberine chloride and 200-1000 ng/spot for guggulsterone Z with regression coefficient value greater than 0.995 for both the phytoconstituents. The method was validated by determining its accuracy, precision, robustness, specificity and system suitability. The method was found to be accurate, precise and robust to carry out the simultaneous estimation of berberine chloride and guggulsterone Z. The developed method was successfully applied for the simultaneous estimation of berberine chloride and guggulsterone Z in herbal formulation.

scholarly journals Development of analytical method and validation for determination of Lisinopril dihydrate in bulk drug and dosage form using HPTLC method

2018 ◽  
Vol 3 (1) ◽  
pp. 1-6
Author(s):  
Sarfaraz Khan ◽  
Furquan N. Khan ◽  
Mohammad Sadeque ◽  
Rana Zainuddin ◽  
Zahid Zaheer
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Scanning Speed ◽  
Application Rate ◽  
Chromatography Method ◽  
Accuracy And Precision ◽  
Bulk Drug ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, reproducible and efficient High Performance Thin Layer Chromatography method was developed for Lisinopril dihydrate in bulk drug and dosage form. A constant application rate of 0.1 ml/s with nitrogen aspirator was used, and the space between two bands was 6 mm. The slit dimension was 5 × 0.45 mm, and the scanning speed was 10 mm/s. The mobile phase consisted of n-butanol: methanol: ammonia in the ratio of 3.0: 1.0: 1.0 (v/v/v). The retention time (min) and linearity range (μl) for Lisinopril was (0.20) and (1-5) respectively. The method so developed was validated for its accuracy and precision. The LOD and LOQ were found to be 0.050237 and 0.152233 for Lisinopril respectively. The accuracy was found to be 98.88%. The developed method was found to be accurate, precise and selective for determination of Lisinopril in bulk and dosage form.

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