scholarly journals Determining Undeclared Synthetic Pharmaceuticals as Adulterants in Weight Loss Herbal Medicines

2020 ◽  
Vol 10 (1) ◽  
pp. 26253
Author(s):  
Babak Salahshour ◽  
Sajjad Sadeghi ◽  
Hajar Nazari ◽  
Kambiz Soltaninejad
Keyword(s):  
Weight Loss ◽  
High Performance ◽  
Herbal Medicines ◽  
Drug Market ◽  
Gas Chromatography Mass Spectrometry ◽  
Array Detector ◽  
Active Pharmaceutical Ingredients ◽  
Diode Array Detector ◽  
Pharmaceutical Ingredients ◽  
Health Authorities

Background: The popularity and use of herbal medicines and supplements are growing worldwide. Herbal anti-obesity products have been considered as suitable alternatives to synthetic pharmaceuticals as they are introduced as harmless natural products. However, some manufacturers often add undeclared synthetic pharmaceuticals to the anti-obesity herbal medicine products to improve their efficacy and potency. The present study aimed to analyze herbal weight loss products collected from the drug market in Bojnurd City, Iran.Methods: Ninety-six herbal drug samples, as weight loss products, were obtained from herb shops and pharmacies in Bojnurd City, Iran. All samples were analyzed to detect undeclared active pharmaceutical ingredients using High-Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD) and Gas Chromatography-Mass Spectrometry (GC-MS) techniques.Results: Caffeine, trimethoxyamphetamine, and vitamin E were identified in herbal weight loss products. Caffeine was detected in 21.8% of the obtained samples, as the most common undeclared active pharmaceutical adulterant. Conclusion: Undeclared active pharmaceutical ingredients in herbal weight loss products could threaten patients’ health. Thus, it is necessary to create awareness through health authorities in this regard.

scholarly journals DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY DETECTOR METHOD TO ANALYSIS OF KAEMPFEROL MARKER FROM EXTRACTS OF POINCIANELLA PYRAMIDALIS (TUL) L.P QUEIROZ

2017 ◽  
Vol 10 (12) ◽  
pp. 252
Author(s):  
Agna Heliade Oliveira ◽  
Valmir Gomesde Souza ◽  
FÁbio Santosde Souza ◽  
Rui Oliveira Macedo
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Herbal Medicines ◽  
Milling Process ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Limit Of Quantification ◽  
Development And Validation

Objective: This study aims to develop the extraction of the marker kaempferol in the fluid extract (FE) and validate an analytical method that monitors the quality of extracts of P. pyramidalis. Methods: The P. pyramidalis leaves were collected and then were dried to milling process. The extracts were drawn up at 20% weight: Volume (w/v) by maceration, and the extraction system used was hydroethanol solution ratio at 50:50 volume: Volume (v: v). From the hydroalcoholic extract, a method of extracting the kaempferol biomarker was developed and validated by high-performance liquid chromatography coupled with diode array detector. To validate a method, the following parameters were evaluated: Specificity, selectivity, linearity, limit of quantification (LOQ) and detection (LOD), precision, accuracy, robustness, and stability. Results: The method developed proved to be efficient, as it allowed the analysis of the interferents free marker, with recovery above 90%, linear over the range 1.4–26.6 μg/mL, correlation coefficient R2=0.999, and LOD and LOQ 0.07 and 0.22 μg/mL, respectively, specificity, precision, accuracy, and robustness. Conclusion: The extraction methodology of the kaempferol marker was successfully developed interferents free and the validated method by HPLC-DAD represents a useful tool in the quality control of P. pyramidalis herbal medicines.

scholarly journals Degradation of benzodiazepines using water falling film dielectric barrier discharge reactor

2017 ◽  
Vol 82 (7-8) ◽  
pp. 933-942
Author(s):  
Vesna Radulovic ◽  
Goran Roglic ◽  
Dragan Manojlovic
Keyword(s):  
Dielectric Barrier Discharge ◽  
High Performance ◽  
Degradation Rate ◽  
Barrier Discharge ◽  
Biologically Active ◽  
Array Detector ◽  
Active Pharmaceutical Ingredients ◽  
Dielectric Barrier ◽  
Pharmaceutical Ingredients ◽  
Dbd Reactor

Classical methods of wastewater treatment are often not suitable for the treatment of pharmaceutical waste. The previous studies have shown that the use of the advanced oxidation procedures (AOP) can lead to a more efficient degradation of various biologically active compounds, which are active pharmaceutical ingredients of applied drugs. The aim of this paper is the application of the plasma technology on the degradation of a two active pharmaceutical ingredients (APIs, diazepam and alprazolam) and the finished products (Bensedin? and Ksalol?) using the dielectric barrier discharge (DBD) reactor for AOP. We studied the degradation rate of these pharmaceuticals, depending on the number of passes through the reactor. This degradation method was efficient 61 % for diazepam and 95 % alprazolam. We also examined the influence of the pH adjustment between the passes of APIs through the DBD reactor. The degradation rate of APIs and the finished products was monitored by the high performance liquid chromatography (HPLC) technique, using a photodiode array detector. The concentration of the dissolved ozone was determined using the iodometric procedure.

Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs

2019 ◽  
Vol 69 (12) ◽  
pp. 3590-3592
Author(s):  
Nela Bibire ◽  
Romeo Iulian Olariu ◽  
Luminita Agoroaei ◽  
Madalina Vieriu ◽  
Alina Diana Panainte ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Fluids ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Assay Method ◽  
Active Pharmaceutical Ingredients ◽  
First Line ◽  
Pharmaceutical Ingredients ◽  
Liquid Chromatographic

Active pharmaceutical ingredients such as isoniazid, pyrazinamide and rifampicin are among the most important first-line anti-tuberculosis drugs. A simple, rapid and sensitive reversed phase-high performance liquid chromatographic assay method for the simultaneous determination of isoniazid, pyrazinamide and rifampicin has been developed. Separation of the interest compounds was achieved in a 10 min chromatographic run in gradient elution mode on a Zorbax SB-C18 stainless steel column (150 � 4 mm, 5 mm) using a guard column containing the same stationary phase. The gradient elution was carried out with a mobile phase of 10% CH3CN aqueous solution for channel A and 50% CH3CN in pH = 6.8 phosphate buffer (20 mM), to which 1.5 mL triethylamine were added for channel B. Quantification of the analyzed substances was carried out spectrophotometrically at 269 nm. Detection limits of 0.48 mg/L for isoniazid, 0.52 mg/L for pyrazinamide and 0.48 mg/L for rifampicin were established for the developed assay method. The present work showed that the proposed analysis method was advantageous for simple and rapid analysis of the active pharmaceutical ingredients in pharmaceuticals and biological fluids.

scholarly journals Simple HPLC-PDA Analysis to Determine Illegal Synthetic Dyes in Herbal Medicines

2021 ◽  
Vol 11 (14) ◽  
pp. 6641
Author(s):  
Kyung-Yuk Ko ◽  
Eun-Young Choi ◽  
Se-Hee Jeong ◽  
Sohwa Kim ◽  
Choon-Kil Lee ◽  
...  
Keyword(s):  
High Performance ◽  
Hplc Analysis ◽  
Ammonium Acetate ◽  
Herbal Medicines ◽  
Positive Sample ◽  
Array Detector ◽  
Synthetic Dyes ◽  
Sunset Yellow ◽  
Herbal Medication ◽  
Relative Standard

Various synthetic dyes are artificially added to herbal medicines for the purpose of visual attraction. In order to monitor the illegal usage of synthetic dyes in herbal medication, a rapid and straightforward analysis method to determine synthetic dyes is required. The study aimed to develop and validate a high-performance liquid chromatography (HPLC) analysis to determine ten synthetic dyes in Hawthorn fruit, Cornus fruit, and Schisandra fruit. Ten synthetic dyes such as Tartrazine, Sunset yellow, Metanil yellow, Auramine O, Amaranth, Orange II, Acid red 73, Amaranth, New Coccine, Azorubine, and Erythrosine B, were extracted using 50 mM ammonium acetate in 70% MeOH; then separated by gradient elution with a mobile phase consisting of acetonitrile and 50 mM ammonium acetate in distilled water using a photodiode array detector (PDA) at 428 nm or 500 nm. In addition, this study established the LC-MS/MS method to confirm the existence of synthetic dyes in the positive sample solution. The HPLC analysis had good linearity (r2 > 0.999). The recoveries of this method ranged from 74.6~132.1%, and the relative standard deviation (RSD) values were less than 6.9%. Most of the samples fulfilled the acceptance criteria of the AOAC guideline. This study demonstrates that the HPLC analysis can be applied to determine ten synthetic dyes in herbal medication.

scholarly journals Analysis of the Mycosporine-Like Amino Acid (MAA) Pattern of the Salt Marsh Red Alga Bostrychia scorpioides

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 321
Author(s):  
Maria Orfanoudaki ◽  
Anja Hartmann ◽  
Julia Mayr ◽  
Félix L. Figueroa ◽  
Julia Vega ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Red Alga ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
The Past ◽  
Specific Species

This study presents the validation of a high-performance liquid chromatography diode array detector (HPLC-DAD) method for the determination of different mycosporine-like amino acids (MAAs) in the red alga Bostrychia scorpioides. The investigated MAAs, named bostrychines, have only been found in this specific species so far. The developed HPLC-DAD method was successfully applied for the quantification of the major MAAs in Bostrychia scorpioides extracts, collected from four different countries in Europe showing only minor differences between the investigated samples. In the past, several Bostrychia spp. have been reported to include cryptic species, and in some cases such as B. calliptera, B. simpliciuscula, and B. moritziana, the polyphyly was supported by differences in their MAA composition. The uniformity in the MAA composition of the investigated B. scorpioides samples is in agreement with the reported monophyly of this Bostrychia sp.

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