Implant surface features as key role on cell behavior

Abstract Objective Treatment of implants with peri-implantitis is often unsuccessful due to residual microbial biofilm hindering re-osseointegration. The aim of this study was to treat biofilm-grown titanium (Ti) implants with different modalities involving air abrasion (AA) and cold atmospheric plasma (CAP) to compare the effectiveness in surface decontamination and the alteration/preservation of surface topography. Materials and methods Saliva collected from a peri-implantitis patient was used to in vitro develop human biofilm over 35 implants with moderately rough surface. The implants were then mounted onto standardized acrylic blocks simulating peri-implantitis defects and treated with AA (erythritol powder), CAP in a liquid medium, or a combination (COM) of both modalities. The remaining biofilm was measured by crystal violet (CV). Surface features and roughness before and after treatment were assessed by scanning electron microscope (SEM). The data were statistically analyzed using Kruskal-Wallis followed by Tukey’s multiple comparison test. Results In the present peri-implantitis model, the human complex biofilm growth was successful as indicated by the statistical significance between the negative and positive controls. All the treatment groups resulted in a remarkable implant surface decontamination, with values very close to the negative control for AA and COM. Indeed, statistically significant differences in the comparison between the positive control vs. all the treatment groups were found. SEM analysis showed no post-treatment alterations on the implant surface in all the groups. Conclusions Decontamination with AA delivering erythritol with or without CAP in liquid medium demonstrated compelling efficacy in the removal of biofilm from implants. All the tested treatments did not cause qualitative alterations to the Ti surface features. No specific effects of the CAP were observed, although further studies are necessary to assess its potential as monotherapy with different settings or in combination with other decontamination procedures. Clinical relevance CAP is a promising option in the treatment of peri-implantitis because it has potential to improve the elimination of bacterial plaque from implant surfaces, in inaccessible pockets or during open-flap debridement, and should stimulate the process of the re-osseointegration of affected dental implants by not altering surface features and roughness.

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Impact of Implant Surface Micropatterns on Epithelial Cell Behavior

Implant Dentistry ◽

10.1097/id.0000000000000621 ◽

2017 ◽

Vol 26 (4) ◽

pp. 600-606 ◽

Cited By ~ 1

Author(s):

Yiping Tong ◽

Zhengyu Yang ◽

Ruoxi Du ◽

Siqian Wang ◽

Qiaozhen Zhou ◽

...

Keyword(s):

Epithelial Cell ◽

Cell Behavior ◽

Implant Surface

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Western Faculty Profile: Dr. Douglas Hamilton

Western Undergraduate Research Journal Health and Natural Sciences ◽

10.5206/wurjhns.2017-18.20 ◽

2017 ◽

Vol 8 (1) ◽

Author(s):

Kevin X Zhou

Keyword(s):

Tissue Engineering ◽

Cell Biology ◽

Scientific Research ◽

Associate Professor ◽

Cell Behavior ◽

Surface Features ◽

Implant Materials ◽

School Of Medicine ◽

The University

Dr. Douglas Hamilton is an Assistant Dean for Research and Associate Professor in Oral Biology and Anatomy & Cell Biology at the Schulich School of Medicine and Dentistry. Hamilton completed his Ph.D. at the University of St. Andrews in Scotland. His research is in the field of cell biology, biomaterials, and tissue engineering, with a focus on how the surface features of different implant materials affect cell behavior. Dr. Hamilton was interviewed for his professional and personal insights on how undergraduates can begin their journey into scientific research.

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Osteoblast maturation and new bone formation in response to titanium implant surface features are reduced with age

Journal of Bone and Mineral Research ◽

10.1002/jbmr.1628 ◽

2012 ◽

Vol 27 (8) ◽

pp. 1773-1783 ◽

Cited By ~ 59

Author(s):

Rene Olivares-Navarrete ◽

Andrew L Raines ◽

Sharon L Hyzy ◽

Jung Hwa Park ◽

Daphne L Hutton ◽

...

Keyword(s):

Bone Formation ◽

Titanium Implant ◽

New Bone Formation ◽

Implant Surface ◽

Surface Features ◽

Osteoblast Maturation ◽

Titanium Implant Surface

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The Geosciences Applied to Lunar Exploration

Symposium - International Astronomical Union ◽

10.1017/s0074180900178222 ◽

1962 ◽

Vol 14 ◽

pp. 169-257 ◽

Cited By ~ 9

Author(s):

J. Green

Keyword(s):

Lunar Surface ◽

Probable Mechanism ◽

Point Of View ◽

Lunar Exploration ◽

Geological Model ◽

Apply Geophysics ◽

Surface Features ◽

The Impact

The term geo-sciences has been used here to include the disciplines geology, geophysics and geochemistry. However, in order to apply geophysics and geochemistry effectively one must begin with a geological model. Therefore, the science of geology should be used as the basis for lunar exploration. From an astronomical point of view, a lunar terrain heavily impacted with meteors appears the more reasonable; although from a geological standpoint, volcanism seems the more probable mechanism. A surface liberally marked with volcanic features has been advocated by such geologists as Bülow, Dana, Suess, von Wolff, Shaler, Spurr, and Kuno. In this paper, both the impact and volcanic hypotheses are considered in the application of the geo-sciences to manned lunar exploration. However, more emphasis is placed on the volcanic, or more correctly the defluidization, hypothesis to account for lunar surface features.

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Extracellular matrix: the gatekeeper of tumor angiogenesis

Biochemical Society Transactions ◽

10.1042/bst20190653 ◽

2019 ◽

Vol 47 (5) ◽

pp. 1543-1555 ◽

Cited By ~ 10

Author(s):

Maurizio Mongiat ◽

Simone Buraschi ◽

Eva Andreuzzi ◽

Thomas Neill ◽

Renato V. Iozzo

Keyword(s):

Extracellular Matrix ◽

Tumor Angiogenesis ◽

Signaling Cascades ◽

Cancer Growth ◽

Cell Behavior ◽

Metastatic Progression ◽

Surface Receptors ◽

The Matrix ◽

Multiple Signaling

Abstract The extracellular matrix is a network of secreted macromolecules that provides a harmonious meshwork for the growth and homeostatic development of organisms. It conveys multiple signaling cascades affecting specific surface receptors that impact cell behavior. During cancer growth, this bioactive meshwork is remodeled and enriched in newly formed blood vessels, which provide nutrients and oxygen to the growing tumor cells. Remodeling of the tumor microenvironment leads to the formation of bioactive fragments that may have a distinct function from their parent molecules, and the balance among these factors directly influence cell viability and metastatic progression. Indeed, the matrix acts as a gatekeeper by regulating the access of cancer cells to nutrients. Here, we will critically evaluate the role of selected matrix constituents in regulating tumor angiogenesis and provide up-to-date information concerning their primary mechanisms of action.

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