scholarly journals Uji Cemaran Mikroba Dalam Susu Kedelai Tanpa Merek Di Kecamatan Jaya Baru Kota Banda Aceh Secara Total Plate Count (TPC)

2019 ◽  
Vol 4 (1) ◽  
pp. 364 ◽  
Author(s):  
Yuni Dewi Safrida ◽  
Raihanaton Raihanaton ◽  
Ananda Ananda
Keyword(s):  
Random Sampling ◽  
Plate Count ◽  
Soy Milk ◽  
Descriptive Research ◽  
Total Plate Count ◽  
Banda Aceh

<p>Soy Milk is white-yellow liquid or kind of beverage that produced by home Industry which should shown about its quality and hygienic to prevented from microba. The purpose of this research is to find out the level of Microba contamination from No-Brand Soy Milk that sold in Jaya Baru Banda Aceh. This research conducted in May 7 th – 12 th 2018 at AKAFARMA Laboratory, Banda Aceh. The sample was No-Brand Soy Milk that taken from six different places and analyzed by using Random Sampling. It was a descriptive research and using TPC as the method. The result shown that first LT TPC was 23×106 colony/ml, second E was 49×106 colony/ml, third LD was 86×106 colony/ml, forth B was 28×107 colony/ml, fifth LB was 26×106 colony/ml and the last PBC was 50×105 colony/ml, it can be conclude that the sixth-samples of Soy  Milk that sold In Jaya Baru, Banda Aceh were not eligible to SNI   tandard, No.06.8-7388-2009 that has 5×104 colony/ml as its TPC toward the Soy Milk extrac.</p>

scholarly journals Uji Total Plate Count (TPC) Bakteri Pada Minuman Teh Poci Homemade di Gampong Batoh Banda Aceh

2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Yuni Dewi Safrida ◽  
Hardiana Hardiana ◽  
Mauliyana Mauliyana
Keyword(s):  
Random Sampling ◽  
Microbial Contamination ◽  
Plate Count ◽  
Total Plate Count ◽  
Hygienic Quality ◽  
Experimental Laboratory ◽  
Banda Aceh

Poci tea is a favorite beverage produced by household industry. Considering its hygienic quality so that it is not contaminated by microbes seems to be an important factor. This study aims to determine the level of microbial contamination in Poci tea produced by household retailers in Batoh, Banda Aceh. This study was conducted at the Pharmacy Laboratory, Academy of Pharmacy and Food Analysts (AKAFARMA) Banda Aceh from 8  to 12 June 2020. The method of this study is    a quantitative experimental laboratory with Total Plate Count (TPC). The samples of this study are seven Poci tea  obtained from Batoh retailers selected by random sampling. The results showed that the seven Poci teas samples were contaminated  microbes, i.e ALMK with a TPC value of 4x103 colonies / ml, AMK with a TPC value of 4x103 colonies / ml, HFK with a TPC value of 2x103 colonies / ml, JLM with a TPC value of 3x103 colonies / ml, KUCB with a TPC value of 4x103 colonies / ml, MHFK with a TPC value of 4x103 colonies / ml, and MHZZ with a TPC value of 2x103 colonies / ml. In conclusion, the seven Poci tea samples sold in Batoh have TPC values that are not permitted by the Regulation of the Head of RI POM No. 16 of 2016. Therefore, this poci tea is not recommended for consumption.

scholarly journals KARAKTERISTIK MUTU KIMIA DAN MIKROBIOLOGI GURITA (Octopus sp.) KERING YANG DIPASARKAN DIKABUPATEN KONAWE UTARA SULAWESI TENGGARA

2020 ◽  
Vol 3 (1) ◽  
pp. 120
Author(s):  
Asman Asman ◽  
Kobajashi Togo Isamu ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Chemical Composition ◽  
Random Sampling ◽  
Descriptive Analysis ◽  
Microbiological Quality ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Plate Count ◽  
Survey Method ◽  
Chemical Test ◽  
Total Plate Count

Chemical and Microbiological Quality Characteristics of Dried Octopus (Octopus sp.) Marketed in North Konawe Regency Southeast Sulawesi ABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, fat, and carbohydrate) and total bacteria (TPC) of dried Octopus. Data analysis of this study used descriptive analysis. The sampling technique of this study are used simple random sampling and used the survey method. The results of the highest chemical composition of the treatment was found at water content  S1 19.89%, ash S3 13.63%, protein S2 46.99%, fat S3 5.82% and carbohydrate S2 10.19%.  The best Total Plate Count (TPC) result was found at S2 3.8 × 104 Cfu/g Keywords: Chemical Test, Octopus (Octopus sp.) Dry, and Total Plate Count (TPC).ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia (kadar air, kadar abu, kadar protein, kadar lemak, dan kadar karbohidrat) dan total bakteri (TPC) terhadap gurita kering. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Teknik sampling yang digunakan pada penelitian ini adalah simple random sampling. Metode yang digunakan pada penelitian ini yaitu metode survei. Hasil penelitian komposisi kimia perlakuan tertinggi kadar air terdapat pada S1 19.89%, kadar abu tertinggi terdapat pada perlakuan S3 13.63%, kadar protein perlakuan tertinggi terdapat pada S2 46.99%, kadar lemak perlakuan tertinggi terdapat pada  S3 5.82% dan kadar karbohidrat perlakuan tertinggi terdapat pada S2 10.19%. Hasil Uji Total Plate Count (TPC) terendah terdapat pada S2 3.8×104 Cfu/g.Kata kunci: Gurita (Octopus sp.) kering, Total Plate Count (TPC) dan Uji Kimia.

scholarly journals TOTAL BAKTERI DAN IDENTIFIKASI Escherichia coli PADA JAJANAN SIOMAY IKAN YANG DIJAJAKAN DI BEBERAPA SD NEGERI DI KOTA KENDARI

2019 ◽  
Vol 2 (2) ◽  
pp. 196
Author(s):  
Wa Ode Rusmianur ◽  
Asnani Asnani ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Escherichia Coli ◽  
Random Sampling ◽  
Sampling Method ◽  
Food Poisoning ◽  
Plate Count ◽  
Foodborne Disease ◽  
Total Plate Count ◽  
Fish Samples ◽  
Descriptive Survey ◽  
Number Of Bacteria

ABSTRACT          Contamination in food may cause foodborne disease, one of them are diarrhea and food poisoning. The cause of contamination in food is microbial contamination. This study aims to determine the presence of contamination Escherichia coli and the number of bacteria on fish siomay, being sold at public elementary school in Kendari city (Kendari  barat, Mandonga, Puwatu and Poasia). This Research used a descriptive survey with random sampling  method to determine the number of bacteria and Escherichia coli using total plate count (TPC) and EMBA medium. The results showed that the number of bacteria were (A) 3,50 x 103CFU/gram, (B) 3,00 x 103 CFU/gram,  (C) 6,67 x 103 CFU/gram dan (D) 8,00 x 103CFU/gram and wasfound Escherichia coli in samples A, C and D, while samples B not found Escherichia coli. This research showed that 3 out of 4 siomay fish samples (80%) contain Escherichia coli and the number of bacteria still according to SNI standar and siomay is still suitable for consumption.Keywords: Escherichia coli. Foodborne disease, Siomay, Total Plate Count (TPC).ABSTRAKAdanya kontaminasi pada pangan jajanan dapat menyebabkan foodborne disease, salah satunya adalah diare dan keracunan pangan. Penyebab kontaminasi pada pangan adalah cemaran mikroba. Penelitian ini bertujuan untuk mengetahui adanya cemaran  Escherichia coli dan jumlah bakteri pada jajanan siomay ikan yang dijajakan di Beberapa Sekolah Dasar Negeri di Kota Kendari (kecamatan Kendari barat, Mandonga, Puwatu dan Poasia). Metode penelitian yaitu Survey deskriptif dengan pengambilan sampel secara acak (random sampling) untuk mengetahui jumlah bakteri dan adanya bakteri Escherichia coli pada makanan jajanan siomay ikan dengan menggunakan metode TPC dan isolasi pada media EMBA. Hasil Penelitian menunjukan rata-rata jumlah bakteri yaitu (A) 3,50 x 103CFU/gram, (B) 3,00 x 103 CFU/gram,  (C) 6,67 x 103 CFU/gram dan (D) 8,00 x CFU/gram ditemukan Escherichia coli pada sampel A, C dan D sedangkan sampel B tidak ditemukan bakteri Escherichia coli. Hal iniMenunjukkan bahwa 3 dari 4 sampel siomay telah tercemar bakteri Escherichia coli sebesar 80% dengan total koloni bakteri masih memenuhi syarat yang ditetapkan SNI siomay ikan dan masih layak dikonsumsi.Kata kunci: Escherichia coli, Foodborne disease, Siomay, Total Plate Count (TPC).

scholarly journals Identifikasi Sakarin, Siklamat, dan Natrium Benzoat Serta Karakteristik Susu Kedelai yang Dijual di Pasar Tradisional Wilayah Jimbaran, Bali Selama Penyimpanan

2021 ◽  
Vol 10 (1) ◽  
pp. 141
Author(s):  
Novelita Olivea Herman ◽  
Ni Luh Ari Yusasrini ◽  
I Nengah Kencana Putra
Keyword(s):  
Sodium Benzoate ◽  
Sampling Technique ◽  
Plate Count ◽  
Survey Method ◽  
Soy Milk ◽  
Sensory Assessment ◽  
Traditional Markets ◽  
Milk Samples ◽  
Total Plate Count

This study aims to identify saccharin, cyclamate, and sodium benzoate in soy milk circulating in traditional markets in the Jimbaran region, determine the percentage of soy milk samples in traditional markets in the Jimbaran region that contain saccharin, cyclamate, and sodium benzoate, and determine the characteristic of milk soybeans in the traditional markets of the Jimbaran region, Bali during storage. This study uses a survey method with saturation sampling technique. Saccharin, cyclamate and sodium benzoate were analyzed qualitatively and quantitatively while the characteristic of soy milk were observed included pH, total plate count, and sensory assessment. Saccharin, cyclamate, sodium benzoate, pH, total plate count, and sensory assessment were analyzed using descriptive methods. The results showed that soy milk in traditional markets in the Jimbaran region did not contain saccharin, 80% used cyclamate, 100% used sodium benzoate, and characteristics of soy milk in traditional markets in Jimbaran, Bali during 8 hours storage has pH between 6.8 to 8.34, as much as 70% of soy milk has total plate count less than 106 CFU/ml, and it is still well consuming because the color has the like criteria, aroma and flavor has the neutral criteria and very not acidic.

scholarly journals KARAKTERISTIK KOMPOSISI KIMIA DAN TOTAL BAKTERI IKAN ROA (Hemirhampus sp.) ASAP YANG DI PASARKAN DI KECAMATAN BUNGKU TENGAH KABUPATEN MOROWALI

2020 ◽  
Vol 3 (1) ◽  
pp. 113
Author(s):  
Sarni Sarni ◽  
Haslianti Haslianti ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Chemical Composition ◽  
Random Sampling ◽  
Descriptive Analysis ◽  
Simple Random Sampling ◽  
Plate Count ◽  
Survey Method ◽  
Test Results ◽  
Total Plate Count ◽  
Total Bacteria ◽  
Treatment Water

Characteristics of Chemical Composition and Total Bacterial of Roa (Hemirhampus sp.) Smoke Marketed in the Bungku District of Central Morowali DistrictABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, and fat) and total bacteria (TPC) of smoked roa fish. Data analysis this study used descriptive analysis. The method used study is the survey method (Simple Random Sampling). The results of the chemical composition of the highest treatment water content P2 13.02%, ash P3 5.65%, protein P3 40.82%. and fat P3 13.87%. The best organoleptic test results were found in P1 with 7.30 appearance, 8.20 odor, 8.00 taste, 7.80 texture, and the best mushroom specifications were at P2 9.00. The lowest total bacteria test was found with 0,83×105 Log CFU/gr.Keywords: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia  (kadar air, kadar abu, kadar protein dan kadar lemak) dan total bakteri (TPC) terhadap ikan roa asap. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Metode yang digunakan dalam penelitian ini yaitu metode survey (Simple Random Sampling). Hasil penelitian komposisi kimia perlakuan tertinggi kadar air P2 13,02%. Kadar abu tertinggi terdapat pada perlakuan P3 5,65%. Kadar protein perlakuan tertinggi terdapat pada P3 40,82%. dan kadar lemak perlakuan tertinggi terdapat pada  P3 13,87%. Hasil penilaian uji organoleptik terbaik terdapat pada P1 sepesifikasi kenampakan 7,30, bau 8,20, rasa 8,00, tekstur 7,80 dan pada spesifikasi jamur nilai terbaik terdapat pada P2 9,00. Total bakteri terendah terdapat pada P3 dengan nilai 0,83×105 Log CFU/gr.Kata kunci: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik

scholarly journals IDENTIFIKASI SAKARIN, SIKLAMAT, DAN NATRIUM BENZOAT SERTA KARAKTERISTIK SUSU KEDELAI YANG DIJUAL DI PASAR TRADISIONAL WILAYAH JIMBARAN, BALI SELAMA PENYIMPANAN

2020 ◽  
Vol 9 (4) ◽  
pp. 468
Author(s):  
Novelita Olivea Herman ◽  
NL. Ari Yusasrini ◽  
I Nengah Kencana Putra
Keyword(s):  
Sodium Benzoate ◽  
Sampling Technique ◽  
Plate Count ◽  
Survey Method ◽  
Soy Milk ◽  
Sensory Assessment ◽  
Traditional Markets ◽  
Milk Samples ◽  
Total Plate Count

This study aims to identify saccharin, cyclamate, and sodium benzoate in soy milk circulating in traditional markets in the Jimbaran region, determine the percentage of soy milk samples in traditional markets in the Jimbaran region that contain saccharin, cyclamate, and sodium benzoate, and determine the characteristic of milk soybeans in the traditional markets of the Jimbaran region, Bali during storage. This study uses a survey method with saturation sampling technique. Saccharin, cyclamate and sodium benzoate were analyzed qualitatively and quantitatively while the characteristic of soy milk were observed included pH, total plate count, and sensory assessment. Saccharin, cyclamate, sodium benzoate, pH, total plate count, and sensory assessment were analyzed using descriptive methods. The results showed that soy milk in traditional markets in the Jimbaran region did not contain saccharin, 80% used cyclamate, 100% used sodium benzoate, and characteristics of soy milk in traditional markets in Jimbaran, Bali during 8 hours storage has pH between 6.8 to 8.34, as much as 70% of soy milk has total plate count less than 106 CFU/ml, and it is still well consuming because the color has the like criteria, aroma and flavor has the neutral criteria and very not acidic.

scholarly journals Uji Angka Lempeng Total dan Kontaminan Koliform pada Susu Kedelai Bermerek yang Beredar di Kota Denpasar

2021 ◽  
Vol 9 (1) ◽  
pp. 141
Author(s):  
Michael Jordi Theofanny ◽  
Ida Bagus Wayan Gunam ◽  
Ni Putu Suwariani
Keyword(s):  
National Standard ◽  
Plate Count ◽  
Most Probable Number ◽  
Soy Milk ◽  
Probable Number ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Sample Testing ◽  
Good For ◽  
Legal Product

This research aimed to testing total plate count and coliform contaminant on legal product soy milk sold in Denpasar city is qualified to Indonesian National Standard (SNI) and safe to consumption. Soy milk has high nutrition and good for growth microorganisms. Microorganisms in soy milk is good for health but be worried have a dangerous microorganisms. Samples of soy milk based on purposive method, tested with total plate count agar with pour plate method and contaminant coliform with most probable number method. The result of testing total plate count, all samples is under maximum of SNI  5 × 104CFU/g. After that soy milk sample testing of coliform contaminant, the result is all samples has negative coliform contaminant. Conclusion of the research is all of the legal product soy milk sold in Denpasar qualified to SNI No. 01-3830-1995 and safe to consumption. Keyword: coliform, Denpasar city, Indonesian national standard, soy milk, total plate count.

scholarly journals GAMBARAN ANGKA LEMPENG TOTAL DAN IDENTIFIKASI BAKTERI Escherichia coli PADA LOLOH BUNGA TELENG DI DESA PENGLIPURAN KECAMATAN BANGLI

2021 ◽  
Vol 9 (1) ◽  
pp. 12-18
Author(s):  
Nyoman Mastra
Keyword(s):  
Escherichia Coli ◽  
Key Words ◽  
Microbiological Quality ◽  
Plate Count ◽  
Sampling Techniques ◽  
E Coli ◽  
Descriptive Research ◽  
Total Plate Count ◽  
Laboratory Examinations

AbstractBackground : Teleng flower herb is a processed beverage made from teleng flower which is popular as a traditional drink from Penglipuran Village. As one of the beverage products, teleng flower herbs have to obtain consent from the BPOM before distributed and consumed. Purpose : this study was conducted to analyze the quality of microbiology of teleng flower herb. The analyzed parameters were total plate count (ALT) and E. coli bacteria identification. Method : this study used descriptive research with laboratory examinations which was conducted at the Bacteriology Laboratory. The 7 samples were obtained from producers with three times repetition so that it became 21 samples with sampling techniques were saturated sampling. Result : The ALT examination results show that 5 samples (71.4%) are not qualify and 2 samples (28.6%) are qualify based on BPOM Regulation Number 16 In 2016. The obtained results of E. coli identification show that there were 4 (57.1%) contain of E. coli pathogens and 3 samples (42.9%) are not contains of E. coli pathogens. It can be concluded that most of the hygiene of teleng flower herb at Penglipuran Village does not meet the requirements. Conclusion : Based on these results, we suggest to the producers of teleng flower herb to maintain the quality of teleng flower herb that they sell. Key words: teleng flower herb, microbiological quality, total plate count, E. coli

scholarly journals Jumlah Total Bakteri Dalam Saluran Pencernaan Ikan Gurami (Osphronemus Gouramy) Dengan Pemberian Beberapa Pakan Komersial Yang Berbeda [Total Of Bacteria In Digestive Organ Of Gurami Fish (Osphronemus Gouramy) By Administering A Different Commercial Feed]

2019 ◽  
Vol 6 (1) ◽  
pp. 21
Author(s):  
Gaby Stephani Rohy, Boedi Setya Rahardja, Agustono
Keyword(s):  
Digestive Organ ◽  
Plate Count ◽  
Fish Feed ◽  
Average Value ◽  
Descriptive Research ◽  
Crude Fiber Content ◽  
Total Plate Count ◽  
Commercial Feed ◽  
Total Bacteria

Abstract Gurami is a fish with a slow growth because of the efficiency of utilization of nutrients and energy contained in the feed is too slow, so it is not enough energy available for growth. Feed components that contribute to the provision of materials and energy for growth are proteins, carbohydrates, and fats. The ability of fish to use depends on the nutrient feed enzymes in the digestive organ. Enzyme producing bacteria to help digest fish feed with the help of enzymes produced by the bacteria are protease, lipase and amylase. Determination of Total Plate Count (TPC) has a population of principles that determine the amount of bacteria found in the digestive organ of fish, because the bacteria are the main factors that cause decomposition. The purpose of this study is to obtain total bacterial or Total Plate Count (TPC) in the digestive organ of Gurami (Osphronemus gouramy) given different feed. This study was conducted in July-August, 2013, in the Bacteriology Laboratory of Microbiology, Faculty of Veterinary Medicine, Airlangga University Surabaya. This research is a descriptive research method of solving the problem by describing the subject matter into a form that is easy to understand and based on facts. The results showed that the highest number of total bacteria in the stomach of each type of feed. However, if the average value is taken and compared between the feed is P1, P2 and P3 obtained an average highest number of total bacteria on P2 feed, the average number of bacteria in a row in P1, P2, and P3 are 71.8 x 107 ; 64.5 x 107; 2.9 x 107 which also has the highest crude fiber content of nutrients in the form of a percentage on the proximate test is 8.03%. Expected that the results of this study further research on the addition of probiotic bacteria in the gastrointestinal organ which will help of growth of carp.

scholarly journals Efek Dekontaminasi Karkas Ayam Pedaging Menggunakan Asam Asetat, Asam Sitrat dan Kombinasinya Terhadap Angka Lempeng Total Campylobacter sp.

2014 ◽  
Vol 14 (2) ◽  
pp. 96-101
Author(s):  
Zalhendra Eka Putra ◽  
Nurliana Nurliana ◽  
Razali Razali
Keyword(s):  
Acetic Acid ◽  
Citric Acid ◽  
Observation Time ◽  
Plate Count ◽  
Standard Plate Count ◽  
Total Plate Count ◽  
Standard Plate ◽  
Poultry Carcass ◽  
Treatment Observation ◽  
Banda Aceh

(Effect of poultry carcas decontamination by acetic acid, citric acid and its combination to total plate count of Campylobacter sp).ABSTRACT. This research aimed to detect the total number of Campylobacter sp. on poultry carcass after decontamination by acetic acid, citric acid and combination of both. This research was conducted in the Laboratory of Veterinary Public Health Syiah Kuala University, Banda Aceh. The research was factorial completely randomized designed. Samples of poultry carcass were obtained from the Lamnyong market, Banda Aceh. Sixty poultry carcasses were divided into three groups of treatment and one group without treatment. Observation of each treatment was five time replicated at 0, 2, 4, 6 and 8 hours after immersion for ± 30 seconds in each of the decontamination material. Observation of Campylobacter sp. was done by inoculating every sample on CM0739 selective Campylobacter Blood free selective agar base and CCDA Selective supplement SR0155E. Measurements of Total Plate Count (TPC) Campylobacter sp according to Standard Plate Count (SPC). Data was variance analyzed (ANOVA) using SPSS. The results of research showed that the growing colonies of Campylobacter sp. were indicated by white colony with surrounded by black zone. Immersion of poultry carcass with acetic acid, citric acid and combination of the both and the observation time had no influence (P0,05) to ALT Campylobacter sp. The research was concluded that carcass to Campylobacter sp. The immersion by acetic acid, citric acid and combination of both and the observation time were not reduce Campylobacter sp. in poultry carcass.  

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