scholarly journals Streptococcus mutans Antibacterial Activity of 0.1% Lysozyme Tooth Paste as an Alternative for Children Below 3 Years Old in Preventing Early Childhood Caries (ECC) (Experimental Laboratory Study)

2021 ◽  
Vol 24 (2) ◽  
pp. 28-34
Author(s):  
Essie Octiara ◽  
Heriandi Sutadi ◽  
Yahwardiah Siregar ◽  
Ameta Primasari
Keyword(s):  
Antibacterial Activity ◽  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Microtiter Plate ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Post Test ◽  
Experimental Laboratory ◽  
Childhood Caries

One of many possible ways of preventing Early Childhood Caries (ECC) is by removing tooth debris. Fluoride tooth paste usage by children under 3 years old must follow recommended quantity, because of its possibility of being ingested which then might cause fluorosis. Lysozyme as an active element in a toothpaste is able to mediate bacterial aggregation and inhibit bacterial adhesion and also activate bacterial autolysin by destructing bacterial cell wall. This study aimed to compared  Streptococcus mutans antimicrobial activity of non-fluoride lysozyme toothpaste of various concentration such as 0.025, 0.05, 0.1, and 0.2 %. This was an experimental research with post test only design. The tested tooth paste was lysozyme-contained with 0.025, 0.05, 0.1 and 0.2% concentrations. Children tooth paste containing fluoride, enzyme tooth paste and 0.2% chlorhexidine became the positive control. Test of S. mutans antibacterial activity used 3 methods: disc diffusion, well, and microtiter plate method. Analytical test used Anova one way with Bonferoni post hoc and p<0.05 significant level. The results showed that all concentrations of lysozyme tooth paste had inhibitory effect on the growth of S. mutans (p<0.05). The highest mean of  S. mutans antibacterial activity in lysozyme tooth paste were on 0.1 and 0.2% concentrations. Conclusion stated that 0.1% concentration of lysozyme tooth paste can be used as an alternative tooth paste for children under 3 years old  

scholarly journals Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1645 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Positive Correlation ◽  
Unstimulated Saliva ◽  
Student’S T ◽  
Total Bacteria ◽  
Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

Streptococcus Mutans Strains in Mother-Child Pairs of Children with Early Childhood Caries

2019 ◽  
Vol 43 (4) ◽  
pp. 252-256 ◽  
Author(s):  
Priya Subramaniam ◽  
Revathy Suresh
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Mutans Streptococci ◽  
Similar Distribution ◽  
Healthy Children ◽  
Only Children ◽  
Polymerase Chain ◽  
Childhood Caries

Objective: Dental caries is both an infectious and transmissible disease. Maternal transfer of Mutans Streptococci occurs at an early age and is important in the initiation of dental caries in children. The aim of this study was to identify certain strains of Streptococcus mutans in mother-child pairs, of children with early childhood caries. Study design: Sixty mother-child pairs of healthy children aged 18–36 months were selected. Mothers with high levels of Streptococcus mutans in their saliva and only children with ECC were included. Dental plaque samples were collected from mother-child pairs. The plaque samples were stored, transferred to the laboratory and analyzed for Streptococcus mutans strains c, f, e and k, present in mother-child pairs using Real time Polymerase Chain Reaction (PCR) technique. Data obtained was subjected to statistical analysis for level of similarity in Streptococcus mutans strains present in mother-child pairs. Results: A similar distribution of Streptococcus mutans strains c, f and k was identified in 28 mother-child pairs. Streptococcus mutans strain e was seen in 18 pairs. Conclusion: Less than 50% of mother-child pairs showed similarity in distribution of Streptococcus mutans strains.

scholarly journals Association of Feeding Methods and Streptococcus mutans Count with Early Childhood Caries: A Cross-sectional Study

2017 ◽  
Vol 10 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Manjunath P Puranik ◽  
Deepa Bullappa ◽  
KR Sowmya ◽  
T Nagarathnamma
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Cross Sectional Study ◽  
Caries Experience ◽  
Sectional Study ◽  
Cross Sectional ◽  
Mothers And Children ◽  
Feeding Methods ◽  
Childhood Caries

ABSTRACT Introduction Early childhood caries (ECC) is a virulent form of dental caries that can destroy the primary dentition of toddlers and preschool children. The aim was to determine the relationship of feeding methods and oral Streptococcus mutans count in 3- to 5-year-old children with ECC. Materials and methods A cross-sectional study was conducted in children aged 3 to 5 years. Participating mothers were interviewed regarding child's demographic profile, educational level and socioeconomic status of parents, past medical and dental history of the mother and child, child's feeding habits, and dietary habits and oral hygiene practices of mother and child. Clinical examination for dental caries was done using the World Health Organization criteria (1997). Salivary samples of mother–child pair were collected to determine the pH, flow rate, and S. mutans count. Statistical tests, such as Student's t-test, analysis of variance, and Pearson's correlation were applied. Results Out of 150 mother–child pair, statistically significant difference in the caries experience was found between mothers and children with high and low S. mutans count. Moderate but statistically significant negative correlation was found between mean decayed, missing, and filled teeth of mothers and mean decayed, extracted and filled teeth (deft) of children with high S. mutans count. Regarding deft, there was no statistically significant difference between children who were exclusively breast fed (7.85 ± 2.94), exclusively bottle-fed (8.67 ± 3.98), and both breast and bottle-fed (7.77 ± 2.91). Conclusion The mean caries experience of mothers and children was 2.66 ± 2.01 and 7.82 ± 2.94 respectively, with decayed component being maximum. Moderate and significant correlation (r = 0.5) was found between S. mutans of mothers and children in saliva. Significant negative correlation was found between mothers and children with high S. mutans count (r = –0.0284; p = 0.046). How to cite this article Bullappa D, Puranik MP, Sowmya KR, Nagarathnamma T. Association of Feeding Methods and Streptococcus mutans Count with Early Childhood Caries: A Cross-sectional Study. Int J Clin Pediatr Dent 2017;10(2):119-125.

Streptococcus mutans isolated from children with severe-early childhood caries form higher levels of persisters

2020 ◽  
Vol 110 ◽  
pp. 104601 ◽  
Author(s):  
Aaron Bottner ◽  
Richard Y. He ◽  
Andrea Sarbu ◽  
S.M. Hashim Nainar ◽  
Delphine Dufour ◽  
...  
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Longitudinal Evaluation of Salivary Iga-S in Children with Early Childhood Caries Before and After Restorative Treatment

2019 ◽  
Vol 43 (4) ◽  
pp. 239-243 ◽  
Author(s):  
Aline dos Santos Letieri ◽  
Liana Bastos Freitas-Fernandes ◽  
Ana Paula Canedo Valente ◽  
Tatiana Kelly da Silva Fidalgo ◽  
Ivete Pomarico Ribeiro de Souza
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Flow Rate ◽  
Total Protein ◽  
Early Childhood Caries ◽  
Dental Treatment ◽  
Restorative Treatment ◽  
Lactobacillus Spp ◽  
Childhood Caries

Background: Our aim was to compare salivary levels of secretory immunoglobulin A (s-IgA) in children with early childhood caries (ECCG) and those who are caries-free (CFG) and verify these levels in a follow-up period after restorative treatment. Materials and methods: We selected 46 systemically healthy children in the complete primary dentition period, who were allocated into two groups: CFG (n = 23) and ECCG (dmf-s &gt; 0; n = 23). Unstimulated whole saliva was obtained at baseline from both groups and during the follow-up period (7 days, 1, 2 and 3 months) in the ECCG group. The s-IgA was measured using an ELISA assay, and total protein was assessed using the Bradford method. We also evaluated the flow rate (mL/min), Streptococcus mutans and Lactobacillus spp. counting using selective media plaques. The data were submitted to statistical analysis using the software SPSS 20.0 (SPSS Inc, IL, USA) with a confidence interval set at 95%. Results: Salivary s-IgA levels were higher in baseline of ECCG than in CFG (p&lt;0.05). No statistically significant differences were observed between s-IgA salivary levels at baseline and the evaluations after dental treatment in ECCG (p&gt;0.05). However, we observed two different changes in s-IgA levels among participants: one group presented s-IgA reduction, and the other group demonstrated its maintenance. It was shown that patients from the ECCG group who presented a reduction in s-IgA levels during follow-up also showed a decrease in Streptococcus mutans and Lactobacillus spp. count (p&lt;0.05), in contrast to patients who did not present this reduction. The flow rate and total protein were similar between groups (p&gt;0.05). Conclusions: The present data support the idea that children with early childhood caries present higher levels of s-IgA in saliva than caries-free children. The restorative dental treatment does not have a significant influence on salivary levels of this immunoglobulin during the follow-up period.

scholarly journals The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Bingchun Li ◽  
Ting Pan ◽  
Huancai Lin ◽  
Yan Zhou
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Antibiofilm Activity ◽  
Curcumin Treatment ◽  
Clinical Strains ◽  
Live Bacteria ◽  
Total Bacteria ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Abstract Background Streptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC). Results The isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865% ± 7.108% in SECC and to 84.059% ± 10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5 min. For 24 h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5 min (t = 4.110, P = 0.015) and in 24 h (t = 3.453, P = 0.014). Long-term (24 h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980 ± 1.156) μm3/μm2 to (20.136 ± 1.042) μm3/μm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5 min curcumin treatment. After 24 h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant. Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

scholarly journals Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1645 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Boy M. Bachtiar
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Early Childhood Caries ◽  
Positive Correlation ◽  
Unstimulated Saliva ◽  
Student’S T ◽  
Total Bacteria ◽  
Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

scholarly journals In Vitro and In Vivo Anti-infective Potential of Thymol Against Early Childhood Caries Causing Dual Species Candida albicans and Streptococcus mutans

2021 ◽  
Vol 12 ◽  
Author(s):  
Arumugam Priya ◽  
Anthonymuthu Selvaraj ◽  
Dass Divya ◽  
Ramalingam Karthik Raja ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Early Childhood Caries ◽  
Galleria Mellonella ◽  
Biological Activities ◽  
Infective Potential ◽  
Childhood Caries

Early childhood caries (ECC), a severe form of caries due to cross-kingdom interaction of Candida albicans and Streptococcus mutans, is a serious childhood dental disease that affects majority of the children with poor background. The present study investigated the anti-infective potential of thymol against C. albicans and S. mutans dual species for the management of ECC. Thymol, a plant derivative of the monoterpene group, has been well known for its numerous biological activities. Thymol at 300 μg/ml concentration completely arrested growth and proliferation of dual species of C. albicans and S. mutans. Rapid killing efficacy of pathogens, within a span of 2 min, was observed in the time kill assay. In addition, at sub-inhibitory concentrations, thymol effectively diminished the biofilm formation and virulence of both C. albicans and S. mutans such as yeast-to-hyphal transition, hyphal-to-yeast transition, filamentation, and acidogenicity and acidurity, respectively, in single and dual species state. qPCR analysis was consistent with virulence assays. Also, through the invertebrate model system Galleria mellonella, in vivo toxicity and efficacy of the phytocompound was assessed, and it was found that no significant toxic effect was observed. Moreover, thymol was found to be proficient in diminishing the infection under single and dual state in in vivo condition. Overall, the results from the present study illustrate the anti-infective potential of thymol against the ECC-causing dual species, C. albicans and S. mutans, and the applicability of thymol in medicated dentifrice formulation.

Sign in / Sign up

Export Citation Format

Share Document