scholarly journals Diagnostic Value of IGFBP-1 Rapid Test and Combined IGFBP-1-AFP in Vaginal Fluid from Premature Rupture of Amniotic Membranes

2016 ◽  
Author(s):  
Aryati Aryati ◽  
Lulut Kusumawati ◽  
Agus Sulistyono
Keyword(s):  
Correlation Coefficient ◽  
Vaginal Discharge ◽  
Rapid Test ◽  
Diagnostic Value ◽  
Vaginal Fluid ◽  
Premature Rupture ◽  
Paper Test ◽  
Rapid Tests ◽  
Litmus Paper ◽  
Amniotic Membranes

Objective: To compare the diagnostic value of IGFBP-1 and combined IGFBP-1-AFP rapid tests in diagnosing premature rupture of membranes (PROM). Method: This study was conducted in Dr. Soetomo Hospital in Surabaya from July to November 2013. The subjects were 52 pregnant women with presumed PROM diagnosis, which was recorded by clinical data and sampling of vaginal discharge swab. The diagnostic value was obtained by comparing the results of IGFBP-1 and combinated IGFBP-1-AFP rapid tests by standard PROM examination namely vaginal pooling, litmus paper test and ferning test. Result: A difference between the diagnostic value of IGFBP-1 and combined IGFBP-1-AFP rapid tests in diagnosing PROM was shown, where the sensitivity and specificity of IGFBP-1 rapid test was 85% and 95%, compared to combined IGFBP-1-AFP rapid test, which was 91% and 95%. The correlation coefficient of combined IGFBP-1-AFP rapid test with standard PROM examination (r=0.841, p=0.000) was higher than the correlation coefficient of IGFBP-1-AFP rapid test with standard PROM examination (r=0.772, p=0.000). Conclusion: Combined IGFBP-1-AFP rapid test has a better diagnostic value than IGFBP-1 rapid test alone. Keywords: combined IGFBP-1-AFP, IGFBP-1, PROM

scholarly journals Alpha Feto Protein in vaginal fluid as a diagnostic marker of Premature rupture of membrane

2018 ◽  
Vol 10 (1) ◽  
pp. 335-338
Author(s):  
Nasreen Sultana ◽  
Shamima Rahman ◽  
Fakhruddin Ahmed ◽  
SK Ashraf Ullah
Keyword(s):  
Gold Standard ◽  
Normal Pregnancy ◽  
Vaginal Fluid ◽  
Sensitive Test ◽  
Premature Rupture ◽  
Paper Test ◽  
Cross Sectional ◽  
Group I ◽  
Alpha Feto Protein ◽  
Group Ii

Background : Premature Rupture of Membrane (PROM) is a significant obstetric emergency where a normal pregnancy suddenly becomes a high risk one. It can cause serious complications of mother and fetus. Patient’s history, per speculum vaginal with Nitrazine paper test and Fern test considered as gold standard for diagnosis of PROM. But these multiple tests are hazardous and time consuming. Therefore Alpha-Feto Protein (AFP) test is a single sensitive test for proper diagnosis of PROM. Objective : To evaluate the accuracy of Alpha-Feto protein in vaginal fluid as a diagnostic tool of PROM. Method : It was a cross sectional study, carried out among 120 pregnant women who had the gestational age between 28th to 40th week of pregnancy. Sample was collected purposively and divided into two groups. In group-I-62 suspected PROM patients were selected who gave the history of per vaginal watery discharge and was found positive in per speculum examination (P/S). In group-II-58 non PROM pregnant patients were selected. Then in group-I, Nitrazine paper test and Fern test were done to confirme PROM patients. Both groups AFP test was done by AxSYM auto analyzer. Finally the accuracy of AFP was evaluated against the gold standard test. Result : It was found that AFP concentration was significantly higher in group-I of suspected PROM patients (30- 502ng/ml) than in group-II (0-40ng/ml). Among 62 suspected PROM patients 49 were found gold standard (Nitrogen paper test and Fern test) positive. Out of these 49 confirmed PROM patients 48 was found positive in Alpha-Feto protein test. The sensitivity of AFP test was found 98%, specificity was 84.6% and accuracy was 95.1%. Conclusion : As Alpha-Feto protein in vaginal fluid was found highly accurate (95.1%) for diagnosis of PROM. Therefore, AFP test can be used as a single sensitive test for diagnosis of PROM. Northern International Medical College Journal Vol.10(1) Jul 2018: 335-338

scholarly journals Diagnostic accuracy of two commercial SARS-CoV-2 Antigen-detecting rapid tests at the point of care in community-based testing centers

2020 ◽  
Author(s):  
Alice Berger ◽  
Marie Therese Ngo Nsoga ◽  
Francisco Javier Perez-Rodriguez ◽  
Yasmine Abi Aad ◽  
Pascale Sattonnet-Roche ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Point Of Care ◽  
Rapid Test ◽  
Diagnostic Value ◽  
Rapid Diagnostic Tests ◽  
Ease Of Use ◽  
Rt Pcr ◽  
Test Device ◽  
Community Based ◽  
Rapid Tests

AbstractBackgroundAntigen-detecting rapid diagnostic tests for SARS-CoV-2 offer new opportunities for the quick and laboratory-independent identification of infected individuals for control of the SARS-CoV-2 pandemic.MethodsWe performed a prospective, single-center, point of care validation of two antigen-detecting rapid diagnostic tests (Ag-RDT) in comparison to RT-PCR on nasopharyngeal swabs.FindingsBetween October 9th and 23rd, 2020, 1064 participants were enrolled. The Panbio™Covid-19 Ag Rapid Test device (Abbott) was validated in 535 participants, with 106 positive Ag-RDT results out of 124 positive RT-PCR individuals, yielding a sensitivity of 85.5% (95% CI: 78.0–91.2). Specificity was 100.0% (95% CI: 99.1–100) in 411 RT-PCR negative individuals. The Standard Q Ag-RDT (SD Biosensor, Roche) was validated in 529 participants, with 170 positive Ag-RDT results out of 191 positive RT-PCR individuals, yielding a sensitivity of 89.0% (95%CI: 83.7–93.1). One false positive result was obtained in 338 RT-PCR negative individuals, yielding a specificity of 99.7% (95%CI: 98.4–100). For individuals presenting with fever 1-5 days post symptom onset, combined Ag-RDT sensitivity was above 95%.InterpretationWe provide an independent validation of two widely available commercial Ag-RDTs, both meeting WHO criteria of ≥80% sensitivity and ≥97% specificity. Although less sensitive than RT-PCR, these assays could be beneficial due to their rapid results, ease of use, and independence from existing laboratory structures. Testing criteria focusing on patients with typical symptoms in their early symptomatic period onset could further increase diagnostic value.FundingFoundation of Innovative Diagnostics (FIND), Fondation privée des HUG, Pictet Charitable Foundation.

scholarly journals Diagnostic accuracy of two commercial SARS-CoV-2 antigen-detecting rapid tests at the point of care in community-based testing centers

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248921
Author(s):  
Alice Berger ◽  
Marie Therese Ngo Nsoga ◽  
Francisco Javier Perez-Rodriguez ◽  
Yasmine Abi Aad ◽  
Pascale Sattonnet-Roche ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Point Of Care ◽  
Rapid Test ◽  
Diagnostic Value ◽  
Ease Of Use ◽  
Lower Sensitivity ◽  
Rt Pcr ◽  
Test Device ◽  
Community Based ◽  
Rapid Tests

Objectives Determine the diagnostic accuracy of two antigen-detecting rapid diagnostic tests (Ag-RDT) for SARS-CoV-2 at the point of care and define individuals’ characteristics providing best performance. Methods We performed a prospective, single-center, point of care validation of two Ag-RDT in comparison to RT-PCR on nasopharyngeal swabs. Results Between October 9th and 23rd, 2020, 1064 participants were enrolled. The PanbioTM Covid-19 Ag Rapid Test device (Abbott) was validated in 535 participants, with 106 positive Ag-RDT results out of 124 positive RT-PCR individuals, yielding a sensitivity of 85.5% (95% CI: 78.0–91.2). Specificity was 100.0% (95% CI: 99.1–100) in 411 RT-PCR negative individuals. The Standard Q Ag-RDT (SD Biosensor, Roche) was validated in 529 participants, with 170 positive Ag-RDT results out of 191 positive RT-PCR individuals, yielding a sensitivity of 89.0% (95%CI: 83.7–93.1). One false positive result was obtained in 338 RT-PCR negative individuals, yielding a specificity of 99.7% (95%CI: 98.4–100). For individuals presenting with fever 1–5 days post symptom onset, combined Ag-RDT sensitivity was above 95%. Lower sensitivity of 88.2% was seen on the same day of symptom development (day 0). Conclusions We provide an independent validation of two widely available commercial Ag-RDTs, both meeting WHO criteria of ≥80% sensitivity and ≥97% specificity. Although less sensitive than RT-PCR, these assays could be beneficial due to their rapid results, ease of use, and independence from existing laboratory structures. Testing criteria focusing on patients with typical symptoms in their early symptomatic period onset could further increase diagnostic value.

DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR

2016 ◽  
Vol 21 (3) ◽  
pp. 261
Author(s):  
Ranti Permatasari ◽  
Aryati Aryati ◽  
Budi Arifah
Keyword(s):  
Hepatitis C ◽  
Predictive Value ◽  
Core Protein ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Hcv Infection ◽  
Antibody Detection ◽  
Hcv Rna ◽  
Hcv Antibody

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to the recipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid test that could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multiple antibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. The samples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infection test using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatography method showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictive value of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody pattern was four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies. Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method has a good diagnostic value.

Neonatal Infection and Premature Rupture of Membranes

PEDIATRICS ◽  
1964 ◽  
Vol 33 (3) ◽  
pp. 467-467
Author(s):  
KIT G. JOHNSON
Keyword(s):  
Group Versus ◽  
Neonatal Infection ◽  
Morbidity And Mortality ◽  
Controlled Study ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Clinical Sepsis ◽  
Amniotic Membranes

As the article by Pryles, et al. entitled "A Controlled Study of the Influence on the Newborn of Prolonged Premature Rupture of the Amniotic Membranes and/or Infection in the Mother," Pediatrics, Vol. 31, pp. 608-622, has not yet received comment in your journal, perhaps it is not too late to ask the authors to fill three hiati which I felt existed in their presentation of this excellent study. As antibiotics apparently were used (according to Table X) in some infants prior to the diagnosis of clinical sepsis, what were the comparative morbidity and mortality results in this group versus the group in which treatment was deferred until the diagnosis of clinical sepsis could be made?

scholarly journals Expression rate of ALK tyrosine kinase and TAG-72 oncoprotein in primary skin melanoma

2018 ◽  
Vol 17 (3) ◽  
pp. 50-54
Author(s):  
K. S. Titov ◽  
D. L. Rotin ◽  
A. M. Kazakov ◽  
O. U. Micheeva ◽  
I. M. Telezhnikova ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Correlation Coefficient ◽  
Detection System ◽  
Simultaneous Detection ◽  
Primary Melanoma ◽  
Diagnostic Value ◽  
Positive Sign ◽  
Skin Melanoma ◽  
Alk Positive ◽  
Lymphoid Infiltration

Objective.Determine the frequency of occurrence of tyrosine kinase expression of the mutated ALK and TAG-72 gene among patients with primary melanoma of the skin, to identify their association with a number of histological parameters, and to assess the diagnostic value of the determination of ALK and TAG-72.Materials and methods.Paraffin blocks with surgical material from 40 patients with primary skin melanoma. For routine histological examination, the material was fixed with 10 % neutral formalin for 24 h, poured into paraffin, sections were prepared with a thickness of 4–5 μm, stained with hematoxylin and eosin. IHC study with monoclonal antibodies D57.3 to ALK was performed on an immunostender – Ventana, with antibodies B72.3 to TAG-72 – on Thermo Fischer. As a detection system used: Envision – for TAG-72 and Ventana – for ALK.Results.ALK mutation was detected in 7 (12 %), TAG-72 – 4 (10 %) cases. Evaluation of the correlation force between the presence of ALK and TAG-72 showed a direct average coupling strength (correlation coefficient was 0.31). A direct correlation of the mean force between the presence of TAG-72 oncoprotein, ALK mutation and ulceration in the patient was found – the correlation coefficient was 0.53 and 0.68, respectively. There was a statistically significant association between the presence of ALK and lymphoid infiltration, which in most cases (57 %) was pronounced (p <0.05).Conclusion.Comparing the positive sign of the expression of ALK – 17.5 % and TAG-72 – 10 %, and the positive of their simultaneous detection – 7.5 %, it can be concluded that further studies to determine their diagnostic value are promising. The presence of severe lymphoid infiltration in ALK-positive patients claims a diagnostic value in primary skin melanoma.

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