Detection Of Pseudomonas Aeruginosaisolated From Burn  Patients At The Teaching Hospital In The-Qarprovmce  And Its Resistance To Antibiotics

From the period from March to August 2016,210 swabs were collected from the burn patients hospitalized indifferent hospitals in Baghdad City: Al-Karama Teaching Hospital,Special Burn Hospital,Central Teaching Laboratories,Child protection Teaching Hospital,Imam Ali Hospital. Out of 210 clinical isolates,42 (37.5 %) had been shown a single isolated of pathogenic bacteria K. pneumoniae and the others were belonged to other bacteria and mixed growth isolates. Identification of all isolates were carried out depending on macroscopic, microscopic characterizations,conventional biochemical tests and Api 20E system.Metallo-β lactamase (MBL) enzymes were screen by two phenotypic methods (Meropenem-EDTA double disks method and Modified Hodg test). Susceptibility testing were used with The following antibiotic disks:Imipenem, Meropenem, Ceftazidime, Cefotaxime, Pipracillin, Gentamicin, Amikacin andCiprofloxacin.The percentage of resistance isolates were as followed:Imipenem (21.42%), Meropenem (19.04%), Ceftazidime (69.04%),Cefotaxime (85.71%),Pipracillin (85.71%),Gentamicin (26.19%),Amikacin (19.04 %) andCiprofloxacin (59.52%).The percentage of the prevalence of blaNDM-1 and blaNDM-2 genes in K.pneumoniae isolates from burn patients in Baghdad hospitals were as followed:20 (100 %) forblaNDM-1 genes and 6 (30 %) for blaNDM-2 genes.

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Autopsy audit of burn patients; the Lagos State University Teaching Hospital, Ikeja experience

Nigerian Journal of Plastic Surgery ◽

10.4314/njpsur.v4i2.40403 ◽

2008 ◽

Vol 4 (2) ◽

Author(s):

IO Fadeyibi ◽

MC Izegbu ◽

AS Benebo ◽

SA Ademiluyi

Keyword(s):

Teaching Hospital ◽

University Teaching ◽

Burn Patients ◽

State University ◽

Lagos State

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Pattern of burn injury admissions at a teaching hospital of Karnataka, India: a three year retrospective study

International Surgery Journal ◽

10.18203/2349-2902.isj20185021 ◽

2018 ◽

Vol 5 (12) ◽

pp. 3930

Author(s):

Krishnamurthy V. R. ◽

Ishwaraprasad G. D. ◽

Sumana M. ◽

Samudyatha U. C.

Keyword(s):

Retrospective Study ◽

Data Base ◽

Teaching Hospital ◽

Burn Injury ◽

Burn Patients ◽

Current Trends ◽

Specialist Services ◽

Number Of Patients ◽

Flame Burn ◽

Review Current

Background: The study aims to review current trends in epidemiology, demographics and pattern of burn injury over three year period.Methods: A retrospective study was done using the records of all burn patients admitted from January 2013 to December 2015 at Teaching Hospital, HIMS Hassan. The records were analysed for socio demographic profile, pattern of injuries and outcome.Results: 390 patients were enrolled in the study. Woman patients marginally outnumbered men (52.6% vs. 47.4%). Accidental burns were 92.8%. 84.6% of the patients sustained burns at their home. Flame burn constituted 62.6 % and scald burns 31% of the total burns.Conclusions: Burns is still an issue of concern. Our study highlights the need for a burns data- base to know the reasons and what measures could prevent the occurrence. Number of patients referred is high indicating the need for specialist services at the District level.

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Experiences of burn patients in Ghana: A qualitative study at the Korle-Bu Teaching Hospital in Accra

Indian Journal of Burns ◽

10.4103/ijb.ijb_18_19 ◽

2019 ◽

Vol 27 (1) ◽

pp. 49

Author(s):

Lily Yarney ◽

Elizabeth Yeboah ◽

Rachael Hansen-Garshong

Keyword(s):

Qualitative Study ◽

Teaching Hospital ◽

Burn Patients

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Antibiogram of Biofilm Producing Bacteria Isolated from Urine of Patients in Three Hospitals in Port Harcourt, Rivers State

Microbiology Research Journal International ◽

10.9734/mrji/2021/v31i530316 ◽

2021 ◽

pp. 5-20

Author(s):

Onyedibia Golden Chukwuma ◽

Williams Janet Olufunmilayo ◽

Douglas Salome Ibietela

Keyword(s):

Teaching Hospital ◽

Bacterial Biofilm ◽

Urine Samples ◽

Diffusion Method ◽

Sensitivity Testing ◽

Resistance To Antibiotics ◽

Port Harcourt ◽

Biofilm Bacteria ◽

Tract Infections ◽

Rivers State

Aim: The aim of this study was to determine the antibiogram of biofilm producing bacteria isolated from urine of patients in three hospitals in Port Harcourt, Rivers State. Study Design: The study employs statistical analysis of the data and interpretation Place and Duration of Study: The study was conducted at three (3) hospitals; University of Port Harcourt Teaching Hospital (UPTH), Meridian Hospital D / line branch (MRD1) and Meridian Hospital Ikoku branch, all located in Port Harcourt, Rivers State. Sample collection was for three (3) months, analysis was carried out daily and it lasted for six (6) months. Methodology: A total of Forty-five (45) urine samples were collected for a period of three (3) months from the three (3) hospitals. The samples were labelled properly, according to date and time of collection. The collected samples were subjected to standard microbiological procedures which includes standard plate counts, identification, biofilm screening, sensitivity testing using Kirby-Bauer disk diffusion method, Phenotypic screening of extended spectrum beta lactamase and molecular characterization of the isolates Results: The results of the bacterial population of urine samples from the hospitals showed that the total heterotrophic bacterial counts for Meridian Hospital D/line (MRD1), Meridian Hospital Ikoku (MRD2) and University of Port Harcourt Teaching Hospital (UPTH) ranged from 4.93 - 6.30 x107cfu/ml. The Total coliform count ranged from 1.89-3.04 x106cfu/ml for Meridian Hospital D/line (MRD1), Meridian Hospital Ikoku (MRD2) and University of Port Harcourt Teaching Hospital (UPTH). Total faecal coliform counts ranged from 0.78-1.11 x105CFU/ml for Meridian Hospital D/line (MRD1), Meridian Hospital Ikoku (MRD2) and University of Port Harcourt Teaching Hospital (UPTH). A total of fifty-eight (58) bacterial isolates were isolated from urine of patients and 36(62.1%) isolates were identified as biofilm producers. The biofilm bacteria identified were 17.2% Staphylococcus,6.9% E. coli, 10.3% Pseudomonas, 6.9% Proteus ,10.3% Bacillus and 10.3% Enterococcus species. Biofilm forming ability of bacteria is considered a virulent factor and it is implicated to being a possible cause of increased resistance to most antibiotics. Varying susceptibility pattern was observed among biofilm isolates. Biofilm bacteria were resistant to several groups of antibiotics. Ofloxacin, Gentamycin, Imipenem and Nitrofurantoin can be used as drug of interest for most bacterial biofilm urinary tract infections. CTX-M and TET A gene were identified in the biofilm bacteria in this study to be possible factors that confer resistance to antibiotics. The presence of icaD and papC gene in the isolates whose genome were studied have been found to be possible factors that confers biofilm producing ability. This study indicates the emergence and rapid spread of biofilm producing bacteria and their resistance to antibiotics. Therefore, strict infection control practices as well as therapeutic guidance for confirmed infections should be rapidly initiated.

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BURN PATIENTS;

The Professional Medical Journal ◽

10.29309/tpmj/2013.20.06.1826 ◽

2013 ◽

Vol 20 (06) ◽

pp. 1042-1047

Author(s):

FIRDOUS KHAN ◽

ASIF SHAH ◽

ABDUL AZIZ JANAN

Keyword(s):

Hospital Stay ◽

Plastic Surgery ◽

Teaching Hospital ◽

Burn Injury ◽

Demographic Data ◽

Total Body Surface Area ◽

Length Of Hospital Stay ◽

Descriptive Study ◽

Burn Patients ◽

Key Factor

Aim: To determine major risk factors of mortality and causes of death in patients presented with burn injury. Study design:Prospective Descriptive Study. Setting and duration: Department of Burns and Plastic Surgery, Khyber Teaching Hospital, Peshawar,Pakistan from April 2008 and June 2012. Methodology: A prospective descriptive study was performed among the patients whoadmitted to the Department of Burns and Plastic Surgery, Khyber Teaching Hospital, Peshawar, Pakistan between April 2008 and June2012. All relative information was collected through a detailed proforma and patient’s treatment files. Patients of any age, any degree ofburns and burns exceeding 10% TBSA were included. Patients presenting after more than one week post burn or patients referred fromother hospitals were excluded. Within this period, demographic data, treatment, and outcomes of treatment were reviewed and analyzed.Survivors and non-survivors among burn patients were compared to define the predictive factors of mortality. Results: Between April2008 and June 2012, 1850 patients were admitted with burn injuries. There were 1150 male patients (62%) and 700 female patients(38%). Mean age was 36 years with range of 1-70 years. Inhalation injuries were present in 45 patients (2.40%). Causes were flameburns (65.0%), electrical burns (15%), scalds (13%) and chemical burns (7.0%). The total body surface area (TBSA) burn ranged from10- 100%, with a mean of 38% TBSA burn. Mean length of hospital stay was 12 days (ranging from 24 hours to 170 days). Mortality ratewas 11.2%. Higher age, larger burn area, wound infection, longer hospital stay and the presence of multi-system organ failuresignificantly predicted increased mortality. Conclusions: Prevention is a key factor in reducing the morbidity and mor tality associatedwith burn injury. A campaign to educate people that burns can be prevented will be important in our community. The prevention of multiorganfailure and septicemia are likely to be more effective than their treatment.

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Incidence and Antibiotics Sensitivity of Multidrug-Resistance of Pseudomonas aeruginosa Isolated from Burn’s Patients and Environmental Samples fromThree Hospitals in Baghdad

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2015.9.2.438 ◽

2015 ◽

Vol 9 (2) ◽

pp. 67-73

Author(s):

Asmaa A. AL-Kaisse ◽

Amina N. AL-Thwani ◽

Amina N. AL-Segar

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Multidrug Resistance ◽

Teaching Hospital ◽

Environmental Samples ◽

Enterobacter Aerogenes ◽

Burn Patients ◽

Burn Units ◽

Antibiotics Sensitivity

Two hundred swab samples were collected from burn patients and burn units for the period, beginningfrom August 2012 to the end of April 2013 from a number of hospitals in Baghdad governorateincluding: Al-Kindi General Teaching Hospital, Al-Yarmuk General Teaching Hospital and Al-Imam AliHospital. The collected samples were cultured on different media and tested biochemically in order to findout the profile of bacteria that colonize burn patients wounds and environment of burn units. The resultsof bacterial culturing revealed that out of 200 samples, 105 samples 52.5% were observed to havebacterial growth (positive samples), while negative samples represented 95(47.5%). The profile of thebacteria in the cultured samples revealed: Pseudomonas aeruginosa 40.95% was the most common isolatefollowed by Staphylococcus aureus 20.0%, Klebsiella pneumoniae 17.1%, Escherichia coli 8.5%,Pseudomonas putida 4.76%, Enterobacter aerogenes 3.80%, Acinetobacter baumannii 2.85% andProteus mirabilis 1.90%. Forty three P. aeruginosa isolates were tested for antibiotic susceptibility. Theresults showed most isolates were potentially resistant to different antibiotics as follow, all isolates 100%had resistance to Ceftriaxone, Cefepime, and Chloramphenicol, and showed high resistance toTobramycin 95.3%, Gentamicin 93.0%, Ceftazidime 88.3%, Cefotaxime 86.0%, Piperacillin 83.7% andAmikacin 79.0%, beside illustrating low resistance to Aztreonam 67.4%, Ciprofloxacin 46.5%, andImipenem 13.9% among these antibiotics, Imipenem was the most effective antibiotic because 86.0% ofthe isolates appeared to be high sensitive to it.

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