scholarly journals Cancer cell cytoskeleton behavior on titanium oxides synthesized through ultrafast pulsed laser irradiation

2021 ◽  
Author(s):  
Chandramouli Chinnakkannu Vijayakumar
Keyword(s):  
Hela Cell ◽  
Cancer Cell ◽  
Drug Carrier ◽  
Cell Signalling ◽  
Fibroblast Cell ◽  
Cell Alignment ◽  
Cell Cytoskeleton ◽  
Oxide Phases ◽  
Scanning Electron Microscope Investigation ◽  
Altered Cell

Conventionally, single phases of TiO2 are used for targeted therapy and a drug carrier systems. In this research a harmonized approach in synthesizing multi-Ti oxide phases in a nanostructure and its ability to control cancer cell cytoskeleton behavior. This modulation of HeLa cancer cell cytoskeleton behaviour including shape of the cell, surface area of the cell, alignment of the cell is diligent by using the combination of TiO, Ti3O, Ti2O phases. Field emission scanning electron microscope investigation (FESEM) revealed that multi-Ti oxide nanostructure revealed a greater reduction of HeLa cell relative to fibroblast cell. This altered cell adhesion was followed by modulation of HeLa cell architecture with significant reduction in actin stress fibers. The intricate combination of multi-Ti oxide nanostructures renders a biomaterial that can precisely alter HeLa cell but not the fibroblast cell behaviour has the potential application of creating a multi-Ti oxide nanostructure for targeted cancer therapy, developing nano patterning devices. This unique interaction of HeLa cancer cell with multi-Ti oxide nanostructure has provided an insight of cell-cell signalling which is the fundamental mechanism in regulating their proliferative characteristics.

scholarly journals Cancer cell cytoskeleton behavior on titanium oxides synthesized through ultrafast pulsed laser irradiation

2021 ◽  
Author(s):  
Chandramouli Chinnakkannu Vijayakumar
Keyword(s):  
Hela Cell ◽  
Cancer Cell ◽  
Drug Carrier ◽  
Cell Signalling ◽  
Fibroblast Cell ◽  
Cell Alignment ◽  
Cell Cytoskeleton ◽  
Oxide Phases ◽  
Scanning Electron Microscope Investigation ◽  
Altered Cell

Conventionally, single phases of TiO2 are used for targeted therapy and a drug carrier systems. In this research a harmonized approach in synthesizing multi-Ti oxide phases in a nanostructure and its ability to control cancer cell cytoskeleton behavior. This modulation of HeLa cancer cell cytoskeleton behaviour including shape of the cell, surface area of the cell, alignment of the cell is diligent by using the combination of TiO, Ti3O, Ti2O phases. Field emission scanning electron microscope investigation (FESEM) revealed that multi-Ti oxide nanostructure revealed a greater reduction of HeLa cell relative to fibroblast cell. This altered cell adhesion was followed by modulation of HeLa cell architecture with significant reduction in actin stress fibers. The intricate combination of multi-Ti oxide nanostructures renders a biomaterial that can precisely alter HeLa cell but not the fibroblast cell behaviour has the potential application of creating a multi-Ti oxide nanostructure for targeted cancer therapy, developing nano patterning devices. This unique interaction of HeLa cancer cell with multi-Ti oxide nanostructure has provided an insight of cell-cell signalling which is the fundamental mechanism in regulating their proliferative characteristics.

Caveolin-1, galectin-3 and lipid raft domains in cancer cell signalling

2015 ◽  
Vol 57 ◽  
pp. 189-201 ◽  
Author(s):  
Jay Shankar ◽  
Cecile Boscher ◽  
Ivan R. Nabi
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cellular Response ◽  
Spatial Organization ◽  
Essential Feature ◽  
Cell Signalling ◽  
Coated Pits ◽  
Signalling Molecules ◽  
Raft Domains

Spatial organization of the plasma membrane is an essential feature of the cellular response to external stimuli. Receptor organization at the cell surface mediates transmission of extracellular stimuli to intracellular signalling molecules and effectors that impact various cellular processes including cell differentiation, metabolism, growth, migration and apoptosis. Membrane domains include morphologically distinct plasma membrane invaginations such as clathrin-coated pits and caveolae, but also less well-defined domains such as lipid rafts and the galectin lattice. In the present chapter, we will discuss interaction between caveolae, lipid rafts and the galectin lattice in the control of cancer cell signalling.

Synthesis and Anticancer Activity of New Hydrazide-hydrazones and Their Pd(II) Complexes

2019 ◽  
Vol 16 (5) ◽  
pp. 522-532 ◽  
Author(s):  
Bedia Kocyigit-Kaymakcioglu ◽  
Senem Sinem Yazici ◽  
Fatih Tok ◽  
Miriş Dikmen ◽  
Selin Engür ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Fibroblast Cell ◽  
Reference Drug ◽  
A549 Cancer Cell Line

Background: Hydrazones, one of the important classes of organic molecules, are pharmaceutical agents comprising –CO-NH-N=CH- group in the structure therefore and exhibiting significant biological activity. Methods: 5-Chloro-N’-[(substituted)methylidene] pyrazine-2-carbohydrazide (3a-g) and their Pd(II) complexes (4a-h) were synthesized and investigated in vitro anticancer activity on A549, Caco2 cancer and normal 3T3 fibroblast cell lines, using the MTT assay. Results: Anticancer activity screening results revealed that some compounds showed remarkable cytotoxic effect. Among them, 5-chloro-N'-[(4-hydroxyphenyl)methylidene] pyrazine-2-carbohydrazide (3c) displayed higher cytotoxic activity against A549 cancer cell line than the reference drug cisplatin. Conclusion: Compound 3c showed high cytotoxic activity against A549 cancer cell line but it showed low cytotoxic effect against normal 3T3 fibroblast cell line. Antiproliferative and antimetastatic effects of 3c were determined by the real-time monitoring of cell proliferative system (RTCA DP). The cell proliferation, metastatic and invasive activities of A549 cells were decreased due to increased concentration of 3c.

scholarly journals Maurocalcine as a Non Toxic Drug Carrier Overcomes Doxorubicin Resistance in the Cancer Cell Line MDA-MB 231

2008 ◽  
Vol 26 (4) ◽  
pp. 836-845 ◽  
Author(s):  
Sonia Aroui ◽  
Narendra Ram ◽  
Florence Appaix ◽  
Michel Ronjat ◽  
Abderraouf Kenani ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Drug Carrier ◽  
Cancer Cell Line ◽  
Doxorubicin Resistance ◽  
Toxic Drug

A NOVEL C1Q DOMAIN-CONTAINING PROTEIN ISOLATED FROM THE MOLLUSK MODIOLUS KURILENSIS RECOGNIZING GLYCANS ENRICHED WITH ACIDIC GALACTANS AND MANNANS IS VERY PROSPECTIVE FOR BIOMEDICAL PURPOSES

2021 ◽  
Vol 1 (19) ◽  
pp. 86-88
Author(s):  
A.V. Grinchenko ◽  
A. Kriegsheim ◽  
N.A. Shved ◽  
A.E. Egorova ◽  
D.V. Ilyaskina ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Comparative Analysis ◽  
Hela Cell ◽  
Cell Lines ◽  
Cancer Cell ◽  
Modiolus Kurilensis ◽  
Cell Phenotypes ◽  
Biomedical Potential

A novel C1qDC bivalve protein from the bivalve Modiolus kurilensis (MkC1qDC-1) was identified, isolated and characterized. MkC1qDC-1 demonstrated the inhibition of HeLa cell proliferation and recognition of some cancer cell phenotypes in comparative analysis on several cell lines, suggesting biomedical potential of this protein.

scholarly journals CD133 Targeted PVP/PMMA Microparticle Incorporating Levamisole for the Treatment of Ovarian Cancer

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 479
Author(s):  
Yu-Chi Wang ◽  
Meng-Yi Bai ◽  
Ying-Ting Yeh ◽  
Sung-Ling Tang ◽  
Mu-Hsien Yu
Keyword(s):  
Ovarian Cancer ◽  
Cell Viability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Model ◽  
Drug Carrier ◽  
Matrix Material ◽  
Free Form ◽  
Ovarian Cancer Cells ◽  
Dose Dependent

Levamisole (LEVA) is used to treat worm infections, but it can also inhibit cancer cell growth by inhibiting the aldehyde dehydrogenase pathway. Therefore, here, we developed a drug carrier targeting CD133, a biomarker overexpressed in ovarian cancer cells. The particle structure and cytotoxicity of the prepared LEVA-containing particles—called LEVA/PVP/PMMA microparticles (MPs) (because it used matrix material polyvinylpyrrolidone (PVP) and poly(methylmethacrylate) (PMMA))—were investigated in the ovarian cancer cell lines SKOV-3 and CP70. The particle size of the MPs was determined to be 1.0–1.5 µm and to be monodispersed. The hydrophilic property of PVP created a porous MP surface after the MPs were soaked in water for 20 min, which aided the leaching of the hydrophilic LEVA out of the MPs. The encapsulation efficiency of LEVA/PVP/PMMA MPs could reach up to 20%. Free-form LEVA released 50% of drugs in <1 h and 90% of drugs in 1 day, whereas the drug release rate of LEVA/PVP/PMMA MPs was much slower; 50% released in 4 h and only 70% of drugs released in 1 day. In the in vitro cell model test, 5 mM free-form LEVA and 0.1 g/mL CD133 targeted LEVA/PVP/PMMA MPs reduced SKOV-3 cell viability by 60%; 0.1 g/mL LEVA/PVP/PMMA MPs was equivalent to a similar dosage of the free drug. In addition, the cytotoxicity of CD133-conjugated LEVA/PVP/PMMA MPs shows a different cytotoxicity response toward cell lines. For SKOV-3 cells, treatment with free-form LEVA or CD133-conjugated LEVA/PVP/PMMA MPs exerted dose-dependent cytotoxic effects on SKOV-3 cell viability. However, CD133-conjugated LEVA/PVP/PMMA MPs demonstrated no significant dose-dependent cytotoxic efficacy toward CP70 cells.

scholarly journals Role of p90RSK in regulating the Crabtree effect: implications for cancer

2013 ◽  
Vol 41 (1) ◽  
pp. 124-126 ◽  
Author(s):  
Emily K. Redman ◽  
Paul S. Brookes ◽  
Marcin K. Karcz
Keyword(s):  
Cancer Cell ◽  
High Glucose ◽  
Cell Metabolism ◽  
Cell Signalling ◽  
Cell Types ◽  
Crabtree Effect ◽  
Glucose Levels ◽  
Mitogen Activated Protein ◽  
Ribosomal S6 Kinase

High glucose inhibits mitochondrial respiration, known as the ‘Crabtree effect’, in cancer cells and possibly other cell types. The upstream pathways regulating this phenomenon are poorly understood. In diabetes, where glucose levels are elevated, the p90RSK (p90 ribosomal S6 kinase) has received much attention as a potential upstream mediator of the effects of high glucose. Evidence is also emerging that p90RSK may play a role in cancer cell signalling, although the role of p90RSK in regulating cancer cell metabolism is unclear. In the present paper, we provide an overview of the Crabtree effect and its relationship to mitochondrial metabolism. Furthermore, preliminary data are presented suggesting a role for p90RSK and its upstream components, the ERK (extracellular-signal-regulated kinase) family of MAPKs (mitogen-activated protein kinases), in the Crabtree effect.

scholarly journals Sustained Release and Cytotoxicity Evaluation of Carbon Nanotube-Mediated Drug Delivery System for Betulinic Acid

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Julia M. Tan ◽  
Govindarajan Karthivashan ◽  
Palanisamy Arulselvan ◽  
Sharida Fakurazi ◽  
Mohd Zobir Hussein
Keyword(s):  
Carbon Nanotubes ◽  
Cell Line ◽  
Drug Carrier ◽  
Drug Loading ◽  
Fibroblast Cell ◽  
Xrd Analysis ◽  
Prolonged Release ◽  
Loading Capacity ◽  
Cytotoxicity Studies

Carbon nanotubes (CNTs) have been widely utilized as a novel drug carrier with promising future applications in biomedical therapies due to their distinct characteristics. In the present work, carboxylic acid-functionalized single-walled carbon nanotubes (f-SWCNTs) were used as the starting material to react with anticancer drug, BA to produce f-SWCNTs-BA conjugate viaπ-πstacking interaction. The conjugate was extensively characterized for drug loading capacity, physicochemical properties, surface morphology, drug releasing characteristics, and cytotoxicity evaluation. The results indicated that the drug loading capacity was determined to be around 20 wt% and this value has been verified by thermogravimetric analysis. The binding of BA onto the surface of f-SWCNTs was confirmed by FTIR and Raman spectroscopies. Powder XRD analysis showed that the structure of the conjugate was unaffected by the loading of BA. The developed conjugate was found to release the drug in a controlled manner with a prolonged release property. According to the preliminaryin vitrocytotoxicity studies, the conjugate was not toxic in a standard fibroblast cell line, and anticancer activity was significantly higher in A549 than HepG2 cell line. This study suggests that f-SWCNTs could be developed as an efficient drug carrier to conjugate drugs for pharmaceutical applications in cancer chemotherapies.

Luteolin-Loaded Spion as a Drug Carrier for Cancer Cell In Vitro

2017 ◽  
Vol 31 (2) ◽  
pp. 467-474 ◽  
Author(s):  
L. Alpsoy ◽  
A. Baykal ◽  
Z. Ü. Akal
Keyword(s):  
Cancer Cell ◽  
Drug Carrier
Sign in / Sign up

Export Citation Format

Share Document