scholarly journals Analisis Aktivitas Antosianin dari Buah Senggani (Melastoma candidum L.), Kulit Kopi (Coffea arabica L.), dan Ubi Jalar Ungu (Ipomea batatas L.) Sebagai Indikator Asam Basa

2021 ◽  
Vol 3 (2) ◽  
pp. 1-6
Author(s):  
Julina Wati ◽  
Hasby Hasby

Penelitian ini bertujuan untuk menganalisis aktivitas senyawa antosianin dari ekstrak buah senggani (Melastoma candidum L.), kulit kopi (Coffee arabica L.), dan ubi jalar ungu (Ipomea batatas L.) sebagai indikator asam basa.Ekstrak ketiga sampel diperoleh dengan cara maserasi. Pengujian ekstrak sampel untuk menganalisis aktivitas senyawa antosianin dilakukan dengan menggunakan Buffer pH 1-12. Pengujian ekstrak sampel sebagai indikator asam basa dilakukan dengan cara titrasi. Pada pengujian aktivitas senyawa antosianin, hasil penelitian menunjukkan bahwa ekstrak buah senggani pada pH 4-5 tidak berwarna, ekstrak kulit kopi pada pH 1-5 berwarna merah, sedangkan pada pH 6-12berwarna biru, bahkan tidak berwarna, serta ekstrak ubi jalar ungu pada pH 1-9berwarna merah dan pada pH 10-12 berubah menjadi warna hijau. Pada pengujian ekstrak sampel sebagai  indikator alami, hasil penelitian menunjukkan bahwa pada titrasi asam lemah-basa kuat menggunakan CH3COOH 0,1 M dan 50 mL NaOH 0,1 Masing-masing ekstrak mengalami titik akhir titrasi yang berbeda-beda, yaitu 14 mL,25 mL, dan 27 mLuntuk ekstrak buah senggani, kulit kopi, dan ubi jalar ungu; sedangkan pada titrasi asam kuat-basa kuat menggunakan HCl 0,1 M dan 50 mL NaOH 0,1 M masing-masing ekstrak mengalami titik akhir titrasi yang berbeda-beda pula, yaitu 19 mL, 23 mL, dan 22 mLuntuk ekstrak buah senggani, kulit kopi, dan ubi jalar ungu, sehingga dapat disimpulkan bahwa ekstrak buah senggani (Melastoma candidum L.), kulit kopi (Coffee arabica L.), dan ubi jalar ungu (Ipomea batatas L.)mengandung antosianin yang dapat digunakan sebagai indikator alami pada titrasi asam-basa yang sifatnya ramah lingkungan, murah, dan tidak beracun.

2020 ◽  
Vol 4 (1) ◽  
pp. 36-55
Author(s):  
Rince Alfia Fadri ◽  
Kesuma Sayuti ◽  
Novizar Nazir ◽  
Irfan Suliansyah

One of the types of coffee cultivated in Solok Regency is Arabica Coffee (Coffea Arabica L). The quality of the coffee produced can be influenced by the proper harvesting and post-harvest process so as to produce ground coffee with good quality. This research is done by the method of observation, descriptive, and hedonic test. Ground coffee produced in Solok Regency has a semi-mechanical harvesting process while the post-harvest process begins with the release of the skin and meat of the fruit, subsequent drying, and release of the skin of horns, sorting and grading, roasted, milling and packaging. This research aims to study the processing and quality of Arabica Ground Coffee in Solok Regency.


BioScience ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Rita Maliza ◽  
Febriofca Galih Yatalaththov ◽  
Haris Setiawan ◽  
Listiatie Budi Utami

Author(s):  
Arif Budiman ◽  
Anggi Lutfhi ◽  
Muchtaridi Muchtaridi

Objective: This research aims to formulate syrup of coffee arabica extract with decaffeination process and stability test of caffein and chlorogenic acid in syrup of coffee arabica extract.Methods: An extraction was conducted by using ethanol 70% with soxhletation methods, and  decaffeination process (using liquid-liquid extraction with ratio of etanol and dicloromethan was 1:1).  Syrup formulations of coffee arabica extract was made with various concentrations of Na CMC (thickening agent) and sucralose (flavoring agent). The stability of syrup was evaluated through organoleptic, pH, viscosity, hedonic test, microbiological test and determinated concentration of caffeine and chlorogenic acid.Result: The result showed that the concentration of caffeine before and after decaffeination process were 3.377 + 0091% and 1.028 + 0.079%. While, chlorogenic acid were 4.159 + 0.163% and 3.019 + 0.138%. Microbiological test showed that no contamination in syrup of coffee arabica extract.  The concentration of caffeine in syrup of coffee arabica extract was 1.070 + 0.150% and chlorogenic acid was 4.432 + 1.98 %.Conclusion: The concentration of caffeine before and after formulation process were 3.019 + 0.138% and 4.432 + 1.986%.  The best formula of coffee arabica extract syrup was the formula that contains 5 mg/ml of coffee arabica extract, 1.5 mg/ml of Na CMC and 0.5 mg/ml of sucralose and  no contamination in syrup of coffee arabica extract. Caffeine content would decrease after the decaffeination process. 


Author(s):  
Teguh Man santoso ◽  
Soedarsianto Soedarsianto ◽  
Syamsul Bachri

AbstractPratylenchus coffeae is a parasitic nematoda that infected the roots of some plants, one of them is coffee. The Infection of Pratylenchus coffeae cause root tissue damage that led to root lession and make root become rotten, it will interfere the ability of roots to absorb water and nutrients in the soil which resulted in the growth of plants. At the moment, control of Pratylenchus coffeae are following integrated pests management (IPM) program, which integrated between the use of coffee resistant clone and application of biological agents. Research on biological control was conducted more intensive, at the moment; due to it is friendlier save against environment and cheaper then using chemical nematicides. The research was conducted to know the effects of Micorrhiza Helper Bacteria (MHB),Pseudomonas diminuta and Bacillus subtilis in suppressing the population of P. coffeaeas well as their effect on growth of coffee seedling.  Coffee arabica (Coffea arabica L.) seedling one moth old were used in the experiment. The experiment prepared with eight treatments and five  replications, as follows: A (Pseudomonas diminuta with density of 108 cfu / ml), B (Pseudomonas diminuta with density of 2x108 cfu / ml), C (Bacillus subtilis with density of 108 cfu / ml ), D (Bacillus subtilis with density 2x108 cfu / ml), E (Carbofuran nematicide 5 g formulation / pot), F (Pseudomonas diminuta and Bacillus subtilis with each density of 108 cfu / ml), K- (Nematoda inoculation but without bacteria and nematicide), K+ (coffee seedling  without any additional treatment). The experiment was conducted for sixteen weeks or about four months. The results of the experiment showed that application of MHB could suppress population of P. coffeae and increase coffee seedling growth significantly. Inoculation of B. subtilis at 108 cfu per seedling suppressed significantly nematoda population of 71.3% compared with untreated seedling but inoculated with nematoda. It was not significant different with carbofuran treatment which could suppress nematoda population by 89.7%. The same result also occur on the treatment of P. diminuta at density level 2.108 cfu/seedling, which could suppress nematoda population by 64.2%. Seedling growth were treated with MHB also significantly increase compared with seedling without treatment and inoculation of nematodas, especially on the treatment of B.subtilis at density level 108 cfu and P.diminuta at density level of 108 cfu, with increasing level of 35.4% and 34.2 %, respectively.Keywords: MHB (Mycorrhiza Helper Bacteria), Arabica coffee (Coffea arabica L.),  Pratylenchus coffeae, Bacillus subtilis, Pseudomonas diminuta.


Irriga ◽  
2009 ◽  
Vol 14 (2) ◽  
pp. 220-232 ◽  
Author(s):  
Moisés Santiago Ribeiro ◽  
Elio Lemos da Silva ◽  
Debora Candeias Marques de Moura ◽  
Antonio Augusto Aguilar Dantas

Coeficientes de cultura (Kc) e crescimento vegetativo de Acaiá Cerrado associados a graus-dia de desenvolvimento  Moisés Santiago Ribeiro; Elio Lemos da Silva; Débora Candeias Marques de Moura; Antônio Augusto Aguilar Dantas2Departamento de Engenharia, Universidade Federal de Lavras, Lavras, MG, [email protected]  1 Resumo Há uma tendência de utilização de variáveis meteorológicas que considerem o efeito climático sobre a duração das fases e desenvolvimento da cultura, como base para estabelecer os coeficientes de cultura (Kc). Este estudo teve por objetivo determinar os Kc e o crescimento vegetativo do cafeeiro (Coffea arabica L.), cultivar Acaiá Cerrado MG-1474, estabelecendo uma relação com graus-dia de desenvolvimento (GDD). O trabalho foi conduzido na área experimental da Universidade Federal de Lavras (UFLA), no município de Lavras-MG. A partir dos valores de evapotranspiração de referência (ETo) e evapotranspiração da cultura (ETc), determinaram-se os Kc. Foram realizadas avaliações de crescimento vegetativo e estabelecida a relação com as somas térmicas ou GDD. Apesar do bom ajuste do modelo linear aos dados de crescimento vegetativo em relação aos GDD, houve o efeito das datas de plantio sobre o crescimento da cultura. Os valores de coeficientes de cultura (Kc) estimados pelo modelo desenvolvido para as condições de Lavras foram de0,73 a 1,25 correspondentes aos intervalos de 700 GDD.         UNITERMOS: Coffea arabica L., balanço hídrico, evapotranspiração máxima.  RIBEIRO, M. S.; SILVA, E. L. da; MOURA, D. C. de M.; DANTAS, A. A. A.COEFFICIENTS OF CULTURE (KC) AND VEGETATIVE GROWTH OF ACAIÁ CERRADO ASSOCIATED TO GROWTH DEGREES-DAY   2 Abstract There is a tendency in using meteorological variables that consider the climatic effect on the durations of the culture development phases in order to establish the crop coefficients. This study aimed to determinate the crop coefficients and the vegetative growth of the Acaiá Cerrado MG 1474 coffee plant (Coffee arabica L.) establishing a relation with the growth degree-day (GDD). The work was carried out in the experimental area of the Department of Engineering of the “Federal University of Lavras”, Lavras, MG. By using the values of the reference evapotranspiration and the crop evapotranspiration, the crop coefficients were determined. Vegetative growth evaluations were made and a relation with the thermal sums or growth degree-day was done. Despite the good adjustement of the third-degree linear model using the data of crop coefficients related to growth degree-day, there was the effect of the planting dates on the culture growth. The crop coefficient values estimated by the model developed for the conditions of Lavras ranged from 0.73 to 1.25, corresponding to the intervals of 700 growth degrees-day.KEYWORDS: Coffea arabica, hydric balance, maximum evapotranspiration


2021 ◽  
Vol 9 (A) ◽  
pp. 356-361
Author(s):  
Sake Juli Martina ◽  
Aznan Lelo ◽  
Dharma Lindarto ◽  
Ratna Akbari Ganie ◽  
Muhammad Ichwan ◽  
...  

BACKGROUND: Diabetes mellitus (DM) continues to increase with currently more than 463 million people in the world live with DM. One of the causes of Type 2 DM (T2DM) is insulin resistance. Determining laboratory-based tests for early identification of T2DM is important. One of the tests developed was the detection of homeostatic model assessment – insulin resistance (HOMA-IR) levels. AIM: Using chlorogenic acid found in coffee leaves as antidiabetic agent for alternative treatments in DM, this research is conducted to understand the effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in decreasing HOMA-IR levels in T2DM rats. MATERIALS AND METHODS: Thirty-five male Wistar rats (Rattus norvegicus) had T2DM induced using a combination of feeding a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kgBW), divided into 7 groups (2 groups that did not receive treatment [K1 and K3] and 5 groups that received treatment [K2, K4, K5, K6, K7]). The extract was administered with dosage 150, 200, and 250 mg/kgBW/day through a nasogastric tube for 30 days. Then, the HOMA-IR value will be obtained by multiplying fasting plasma insulin by fasting plasma glucose, then divide by a constant of 22.5. RESULTS: The study showed a significant difference before and after treatment, p value was < 0.05, which a decrease HOMA-IR levels (p = 0.002) after given Coffee Arabica Gayo Leaf Extract at 200 and 250 mg/kgBW/day to Wistar rats for 30 days. The decrease HOMA-IR levels are greater than The T2DM group that received metformin and group without treatment. CONCLUSION: Coffee Arabica Gayo Leaf Extract can significantly decrease HOMA-IR levels at a dose of 200 and 250 mg/kgBW. The decrease of HOMA-IR levels is greater than The T2DM group that received metformin and group without treatment.


2021 ◽  
Vol 9 (T3) ◽  
pp. 294-297
Author(s):  
Sake Juli Martina ◽  
Aznan Lelo ◽  
Dharma Lindarto ◽  
Ratna Akbari Ganie ◽  
Muhammad Ichwan ◽  
...  

BACKGROUND: Coffee arabica gayo leaf extract (Coffea arabica L.) contains polyphenols (chlorogenic acid), flavonoids, saponins, tannins, and steroids which improved PI3K serum levels in rats type 2 diabetic rat. AIM: The purpose of the study was to determine the effect of Coffee Arabica Gayo Leaf Extract (C. arabica L.) on increased PI3K serum levels in type-2 diabetic rat (Rattus norvegicus). METHODS: Thirty-five male Wistar rats with type 2 diabetic induced a combination of feeding a high-fat diet for 5 weeks and intraperitoneal injection of low dose streptozotocin (30 mg/kg). The diabetic rats were randomly divided into seven groups which consist of group without type 2 diabetic, group without type 2 diabetic with dose 250 mg/kg/day Coffee Arabica Gayo Leaf Extract, group with type 2 diabetic without intervention, group with type 2 diabetic with metformin, and group with type 2 diabetic with Coffee Arabica Gayo Leaf Extract doses 150, 200, and 250 mg/kg/day. The extract was administered orally for 30 days. Subsequently, PI3K serum levels were measure by Sandwich Elisa. RESULTS: There were significantly higher PI3K serum levels in the treatment groups than in the control groups (p = 0.037) after giving Coffee Arabica Gayo Leaf Extract 200 mg/kg/day and 250 mg/kg/day to rats for 30 days. CONCLUSION: These data suggest that Coffee Arabica Gayo Leaf Extract can improve PI3K serum levels in a rat model type 2 diabetic.


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