Biologically-induced precipitation of minerals in a medium with zinc under sulfate-reducing conditions

Sulfate-reducing microbial communities were enriched from soils collected in areas with crude-oil exploitation. Cultures were grown in modified Postgate C medium and minimal medium, with ethanol or lactate as an electron donor. The batch cultures were grown with addition of zinc in concentrations of 100-700 mg/l. A lack of increased protein concentration in the solutions compared with the control batch, was noted in cultures containing over 200 mg Zn2+/l. The 16S rRNA method was applied to determine the specific composition of the selected microorganism communities. The analysis indicated the presence of Desulfovibrio spp., Desulfobulbus spp. and Desulfotomaculum spp. in the communities. Diffractometric analysis indicated the presence of biogenic sphalerite in cultures with 100 and 200 mg Zn2+/l and elemental sulfur in cultures with 200 mg Zn2+/l. Other post culture sediments (300-700 mg Zn2+/l) contained only hopeite [Zn3(PO4)2·4H2O] formed abiotically during the experiment, which was confirmed by studies of the activity of sulfate-reducing microbial communities.

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Anaerobic benzene mineralization by natural microbial communities from Niger Delta

Biodegradation ◽

10.1007/s10532-020-09922-x ◽

2020 ◽

Author(s):

Samuel C. Eziuzor ◽

Matthias Schmidt ◽

Carsten Vogt

Keyword(s):

Microbial Communities ◽

Niger Delta ◽

Nitrilotriacetic Acid ◽

Sulfate Reducing ◽

Anaerobic Hydrocarbon Degradation ◽

Reducing Conditions ◽

Helium Ion ◽

Methyl Coenzyme M Reductase ◽

Microbial Morphotypes ◽

Generation Sequencing

AbstractThe Niger Delta is one of the most damaged ecosystems in the world, mainly due to petroleum contamination by oil exploration accidents. We investigated the natural attenuation potential of Niger Delta subsurface sediment samples for anaerobic hydrocarbon degradation using benzene as a model compound under iron-reducing, sulfate-reducing, and methanogenic conditions. Benzene was slowly mineralized under methanogenic and iron-reducing conditions using nitrilotriacetic acid (NTA)-Fe(III), or poorly crystalline Fe(III) oxyhydroxides as electron acceptors, analyzed by measurement of 13CO2 produced from added 13C-labelled benzene. Highest mineralization rates were observed in microcosms amended with Fe(III) oxyhydroxides. The microbial communities of benzene-mineralizing enrichment cultures were characterized by next-generation sequencing of the genes coding for 16S rRNA and methyl coenzyme M reductase A (mcrA). Abundant phylotypes were affiliated to Betaproteobacteriales, Ignavibacteriales, Desulfuromonadales, and Methanosarcinales of the genera Methanosarcina and Methanothrix, illustrating that the enriched benzene-mineralizing communities were diverse and may contain more than a single benzene degrader. The diversity of the microbial communities was furthermore confirmed by scanning helium-ion microscopy which revealed the presence of various rod-shaped as well as filamentous microbial morphotypes.

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Anaerobic oxidation of petroleum hydrocarbons in enrichment cultures from sediments of the Gorevoy Utes natural oil seep under methanogenic and sulfate-reducing conditions

10.21203/rs.3.rs-400517/v1 ◽

2021 ◽

Author(s):

Olga N. Pavlova ◽

Oksana N. Izosimova ◽

Svetlana M. Chernitsyna ◽

Vyacheslav G. Ivanov ◽

Tatyana V. Pogodaeva ◽

...

Keyword(s):

Microbial Communities ◽

Petroleum Hydrocarbons ◽

Electron Acceptors ◽

Rrna Gene ◽

Sulfate Reducing ◽

Enrichment Cultures ◽

Reducing Conditions ◽

Natural Oil ◽

Oil Seep ◽

Natural Oil Seep

Abstract This article presents the first experimental data on the ability of microbial communities from sediments of the Gorevoy Utes natural oil seep to degrade petroleum hydrocarbons under anaerobic conditions. Like in marine ecosystems associated with oil discharge, available electron acceptors, in particular sulfate ions, affect the composition of the microbial community and the degree of hydrocarbon conversion. The cultivation of the surface sediments under sulfate-reducing conditions led to the formation of a more diverse bacterial community and greater loss of n-alkanes (28%) in comparison to methanogenic conditions (6%). Microbial communities of both surface and deep sediments are more oriented to degrade polycyclic aromatic hydrocarbons (PAHs), to which the degree of the PAH conversion testifies (up to 46%) irrespective of the present electron acceptors. Uncultured microorganisms with the closest homologues from thermal habitats, sediments of mud volcanoes and environments contaminated with hydrocarbons mainly represented microbial communities of enrichment cultures. The members of the phyla Firmicutes, Chloroflexi, and Caldiserica (OP5), as well as the class Deltaproteobacteria and Methanomicrobia, were mostly found in enrichment cultures and belong to the “core” of microorganisms The influence of gas-saturated fluids may be responsible for the presence in the bacterial 16S rRNA gene libraries of the sequences of “rare taxa”: Planctomycetes, Ca. Atribacteria (OP9), Ca. Armatimonadetes (OP10), Ca. Latescibacteria (WS3), Ca. division (AC1), Ca. division (OP11), and Ca. Parcubacteria (OD1), which can be involved in hydrocarbon oxidation.

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Faculty Opinions recommendation of Comparison of mechanisms of alkane metabolism under sulfate-reducing conditions among two bacterial isolates and a bacterial consortium.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1001941.375085 ◽

2006 ◽

Author(s):

Peter Williams

Keyword(s):

Bacterial Consortium ◽

Bacterial Isolates ◽

Sulfate Reducing ◽

Reducing Conditions

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Cadmium sorption by EPSs produced by anaerobic sludge under sulfate-reducing conditions

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2006.05.092 ◽

2006 ◽

Vol 138 (3) ◽

pp. 589-593 ◽

Cited By ~ 83

Author(s):

Daoyong Zhang ◽

Jianlong Wang ◽

Xiangliang Pan

Keyword(s):

Anaerobic Sludge ◽

Sulfate Reducing ◽

Reducing Conditions

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Effect of PAHs on nitrogen-fixing and sulfate-reducing microbial communities in seagrass Enhalus acoroides sediment

Archives of Microbiology ◽

10.1007/s00203-021-02321-7 ◽

2021 ◽

Author(s):

Juan Ling ◽

Weiguo Zhou ◽

Qingsong Yang ◽

Xiancheng Lin ◽

Ying Zhang ◽

...

Keyword(s):

Microbial Communities ◽

Nitrogen Fixing ◽

Sulfate Reducing ◽

Enhalus Acoroides

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Control of sulfate reduction by molybdate in anaerobic digestion

Water Science & Technology ◽

10.2166/wst.1997.0441 ◽

1997 ◽

Vol 36 (12) ◽

pp. 143-150 ◽

Cited By ~ 10

Author(s):

Shuzo Tanaka ◽

Young-Ho Lee

Keyword(s):

Anaerobic Digestion ◽

Sulfate Reduction ◽

Methane Production ◽

Phase 1 ◽

Continuous Operation ◽

Phase 2 ◽

Sulfate Reducing ◽

Start Up ◽

Reducing Bacteria ◽

Control Batch

Control of sulfate reduction by adding molybdate was investigated to enhance the methane production under batch and continuous operation in the anaerobic digestion of a sulfate-rich lysine wastewater. In phase 1 of the continuous operation, four anaerobic filters were fed with the lysine wastewater and then added with molybdate at 1,3,5 and 10 mM just after methane producing bacteria (MPB) were completely inhited by H2S produced by sulfate reducing bacteria (SRB). In phase 2, three anaerobic filters were operated with continuous or intermittent addition of 3 mM molybdate from the beginning of operation, including one with no molybdate as a control. Batch experiments revealed that the sulfate reduction was strongly inhibited and finally ceased by adding 3 mM or more of molybdate, resulting in great enhancement of the methane production. In phase 1 of the continuous experiments, all reactors showed the cessation of the methane production when the content of H2S reached 9–10 % in biogas, but the MPB activity was gradually recovered after initiating the molybdate addition at 3 or 5 mM. The 10 mM dosage of molybdate, however, had an inhibiting effect to MPB as well as SRB, resulting in the accumulation of acetate within the reactor. In phase 2, the control reactor continued to decrease the methane production, and a methane conversion rate was only 3 % in the control, while 35 and 10 % in continuously-added and intermittently-added reactors, respectively. Thus, it was confirmed that the MPB activity was greatly enhanced under control of the SRB activity by the continuous addition of molybdate. Comparing phase 2 with phase 1, addition from the start-up of the process is considered more effective than addition after the methane production dropped in the control of the sulfate reduction by molybdate.

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