scholarly journals Anaerobic benzene mineralization by natural microbial communities from Niger Delta

2020 ◽  
Author(s):  
Samuel C. Eziuzor ◽  
Matthias Schmidt ◽  
Carsten Vogt
Keyword(s):  
Microbial Communities ◽  
Niger Delta ◽  
Nitrilotriacetic Acid ◽  
Sulfate Reducing ◽  
Anaerobic Hydrocarbon Degradation ◽  
Reducing Conditions ◽  
Helium Ion ◽  
Methyl Coenzyme M Reductase ◽  
Microbial Morphotypes ◽  
Generation Sequencing

AbstractThe Niger Delta is one of the most damaged ecosystems in the world, mainly due to petroleum contamination by oil exploration accidents. We investigated the natural attenuation potential of Niger Delta subsurface sediment samples for anaerobic hydrocarbon degradation using benzene as a model compound under iron-reducing, sulfate-reducing, and methanogenic conditions. Benzene was slowly mineralized under methanogenic and iron-reducing conditions using nitrilotriacetic acid (NTA)-Fe(III), or poorly crystalline Fe(III) oxyhydroxides as electron acceptors, analyzed by measurement of 13CO2 produced from added 13C-labelled benzene. Highest mineralization rates were observed in microcosms amended with Fe(III) oxyhydroxides. The microbial communities of benzene-mineralizing enrichment cultures were characterized by next-generation sequencing of the genes coding for 16S rRNA and methyl coenzyme M reductase A (mcrA). Abundant phylotypes were affiliated to Betaproteobacteriales, Ignavibacteriales, Desulfuromonadales, and Methanosarcinales of the genera Methanosarcina and Methanothrix, illustrating that the enriched benzene-mineralizing communities were diverse and may contain more than a single benzene degrader. The diversity of the microbial communities was furthermore confirmed by scanning helium-ion microscopy which revealed the presence of various rod-shaped as well as filamentous microbial morphotypes.

Anaerobic Hydrocarbon Degradation in Petroleum-Contaminated Harbor Sediments under Sulfate-Reducing and Artificially Imposed Iron-Reducing Conditions

1996 ◽  
Vol 30 (9) ◽  
pp. 2784-2789 ◽  
Author(s):  
John D. Coates ◽  
Robert T. Anderson ◽  
Joan C. Woodward ◽  
Elizabeth J. P. Phillips ◽  
Derek R. Lovley
Keyword(s):  
Hydrocarbon Degradation ◽  
Sulfate Reducing ◽  
Anaerobic Hydrocarbon Degradation ◽  
Reducing Conditions ◽  
Harbor Sediments

scholarly journals Biologically-induced precipitation of minerals in a medium with zinc under sulfate-reducing conditions

2015 ◽  
Vol 64 (2) ◽  
pp. 149-155 ◽  
Author(s):  
DOROTA WOLICKA ◽  
ANDRZEJ BORKOWSKI ◽  
URSZULA JANKIEWICZ ◽  
WOJCIECH STĘPIEŃ ◽  
PAWEŁ KOWALCZYK
Keyword(s):  
Microbial Communities ◽  
Protein Concentration ◽  
Minimal Medium ◽  
Elemental Sulfur ◽  
Batch Cultures ◽  
Sulfate Reducing ◽  
Specific Composition ◽  
Reducing Conditions ◽  
Oil Exploitation ◽  
Control Batch

Sulfate-reducing microbial communities were enriched from soils collected in areas with crude-oil exploitation. Cultures were grown in modified Postgate C medium and minimal medium, with ethanol or lactate as an electron donor. The batch cultures were grown with addition of zinc in concentrations of 100-700 mg/l. A lack of increased protein concentration in the solutions compared with the control batch, was noted in cultures containing over 200 mg Zn2+/l. The 16S rRNA method was applied to determine the specific composition of the selected microorganism communities. The analysis indicated the presence of Desulfovibrio spp., Desulfobulbus spp. and Desulfotomaculum spp. in the communities. Diffractometric analysis indicated the presence of biogenic sphalerite in cultures with 100 and 200 mg Zn2+/l and elemental sulfur in cultures with 200 mg Zn2+/l. Other post culture sediments (300-700 mg Zn2+/l) contained only hopeite [Zn3(PO4)2·4H2O] formed abiotically during the experiment, which was confirmed by studies of the activity of sulfate-reducing microbial communities.

scholarly journals Anaerobic oxidation of petroleum hydrocarbons in enrichment cultures from sediments of the Gorevoy Utes natural oil seep under methanogenic and sulfate-reducing conditions

2021 ◽  
Author(s):  
Olga N. Pavlova ◽  
Oksana N. Izosimova ◽  
Svetlana M. Chernitsyna ◽  
Vyacheslav G. Ivanov ◽  
Tatyana V. Pogodaeva ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Petroleum Hydrocarbons ◽  
Electron Acceptors ◽  
Rrna Gene ◽  
Sulfate Reducing ◽  
Enrichment Cultures ◽  
Reducing Conditions ◽  
Natural Oil ◽  
Oil Seep ◽  
Natural Oil Seep

Abstract This article presents the first experimental data on the ability of microbial communities from sediments of the Gorevoy Utes natural oil seep to degrade petroleum hydrocarbons under anaerobic conditions. Like in marine ecosystems associated with oil discharge, available electron acceptors, in particular sulfate ions, affect the composition of the microbial community and the degree of hydrocarbon conversion. The cultivation of the surface sediments under sulfate-reducing conditions led to the formation of a more diverse bacterial community and greater loss of n-alkanes (28%) in comparison to methanogenic conditions (6%). Microbial communities of both surface and deep sediments are more oriented to degrade polycyclic aromatic hydrocarbons (PAHs), to which the degree of the PAH conversion testifies (up to 46%) irrespective of the present electron acceptors. Uncultured microorganisms with the closest homologues from thermal habitats, sediments of mud volcanoes and environments contaminated with hydrocarbons mainly represented microbial communities of enrichment cultures. The members of the phyla Firmicutes, Chloroflexi, and Caldiserica (OP5), as well as the class Deltaproteobacteria and Methanomicrobia, were mostly found in enrichment cultures and belong to the “core” of microorganisms The influence of gas-saturated fluids may be responsible for the presence in the bacterial 16S rRNA gene libraries of the sequences of “rare taxa”: Planctomycetes, Ca. Atribacteria (OP9), Ca. Armatimonadetes (OP10), Ca. Latescibacteria (WS3), Ca. division (AC1), Ca. division (OP11), and Ca. Parcubacteria (OD1), which can be involved in hydrocarbon oxidation.

scholarly journals Cyanobacterial Mats in Calcite-Precipitating Serpentinite-Hosted Alkaline Springs of the Voltri Massif, Italy

2020 ◽  
Vol 9 (1) ◽  
pp. 62
Author(s):  
Aysha Kamran ◽  
Kathrin Sauter ◽  
Andreas Reimer ◽  
Theresa Wacker ◽  
Joachim Reitner ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Microbial Mats ◽  
Environmental Dna ◽  
Cyanobacterial Mats ◽  
Cyanobacterial Community ◽  
Mineral Precipitation ◽  
Community Based ◽  
Specific Adaptation ◽  
Carbonate Buildups ◽  
Generation Sequencing

(1) Background: Microbial communities in terrestrial, calcifying high-alkaline springs are not well understood. In this study, we investigate the structure and composition of microbial mats in ultrabasic (pH 10–12) serpentinite springs of the Voltri Massif (Italy). (2) Methods: Along with analysis of chemical and mineralogical parameters, environmental DNA was extracted and subjected to analysis of microbial communities based upon next-generation sequencing. (3) Results: Mineral precipitation and microbialite formation occurred, along with mat formation. Analysis of the serpentinite spring microbial community, based on Illumina sequencing of 16S rRNA amplicons, point to the relevance of alkaliphilic cyanobacteria, colonizing carbonate buildups. Cyanobacterial groups accounted for up to 45% of all retrieved sequences; 3–4 taxa were dominant, belonging to the filamentous groups of Leptolyngbyaceae, Oscillatoriales, and Pseudanabaenaceae. The cyanobacterial community found at these sites is clearly distinct from creek water sediment, highlighting their specific adaptation to these environments.

Microbial Communities’ Characterization in Urban Recreational Surface Waters Using Next Generation Sequencing

2021 ◽  
Author(s):  
Laura Vega ◽  
Jesús Jaimes ◽  
Duvan Morales ◽  
David Martínez ◽  
Lissa Cruz-Saavedra ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Microbial Communities ◽  
Surface Waters ◽  
Next Generation ◽  
Generation Sequencing

Cadmium sorption by EPSs produced by anaerobic sludge under sulfate-reducing conditions

2006 ◽  
Vol 138 (3) ◽  
pp. 589-593 ◽  
Author(s):  
Daoyong Zhang ◽  
Jianlong Wang ◽  
Xiangliang Pan
Keyword(s):  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Reducing Conditions

scholarly journals Structure of Microbial Communities When Complementary Effluents Are Anaerobically Digested

2021 ◽  
Vol 11 (3) ◽  
pp. 1293
Author(s):  
Ana Eusébio ◽  
André Neves ◽  
Isabel Paula Marques
Keyword(s):  
Anaerobic Digestion ◽  
Microbial Communities ◽  
Volatile Fatty Acids ◽  
Olive Mill Wastewater ◽  
Organic Load ◽  
Rrna Gene ◽  
Bacterial Populations ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Olive Mill

Olive oil and pig productions are important industries in Portugal that generate large volumes of wastewater with high organic load and toxicity, raising environmental concerns. The principal objective of this study is to energetically valorize these organic effluents—piggery effluent and olive mill wastewater—through the anaerobic digestion to the biogas/methane production, by means of the effluent complementarity concept. Several mixtures of piggery effluent were tested, with an increasing percentage of olive mill wastewater. The best performance was obtained for samples of piggery effluent alone and in admixture with 30% of OMW, which provided the same volume of biogas (0.8 L, 70% CH4), 63/75% COD removal, and 434/489 L CH4/kg SVin, respectively. The validation of the process was assessed by molecular evaluation through Next Generation Sequencing (NGS) of the 16S rRNA gene. The structure of the microbial communities for both samples, throughout the anaerobic process, was characterized by the predominance of bacterial populations belonging to the phylum Firmicutes, mainly Clostridiales, with Bacteroidetes being the subdominant populations. Archaea populations belonging to the genus Methanosarcina became predominant throughout anaerobic digestion, confirming the formation of methane mainly from acetate, in line with the greatest removal of volatile fatty acids (VFAs) in these samples.

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