New effective strains of nodule bacteria Bradyrhizobium japonicum and Mesorhizobium ciceri

Nodule Bacteria ◽

Functional Characteristics ◽

Northern Latitudes ◽

Positive Effect

The positive effect of strains of nodule bacteria (Bradyrhizobium japonicum (Kirchner 1896) Jordan 1982) on the structural and functional characteristics of soy has been revealed.

pyd Genes of Rhizobium sp. Strain TAL1145 Are Required for Degradation of 3-Hydroxy-4-Pyridone, an Aromatic Intermediate in Mimosine Metabolism

Journal of Bacteriology ◽

10.1128/jb.187.13.4480-4487.2005 ◽

2005 ◽

Vol 187 (13) ◽

pp. 4480-4487 ◽

Cited By ~ 12

Author(s):

Jonathan D. Awaya ◽

Paul M. Fox ◽

Dulal Borthakur

Keyword(s):

Abc Transporter ◽

Sinorhizobium Meliloti ◽

Root Nodule ◽

Structural Genes ◽

Nodule Bacteria ◽

Mesorhizobium Loti ◽

Root Nodule Bacteria ◽

Low Levels ◽

Rhizobium Sp

ABSTRACT Rhizobium sp. strain TAL1145 degrades the Leucaena toxin mimosine and its degradation product 3-hydroxy-4-pyridone (HP). The aim of this investigation is to characterize the Rhizobium genes for HP degradation and transport. These genes were localized by subcloning and mutagenesis on a previously isolated cosmid, pUHR263, containing mid genes of TAL1145 required for mimosine degradation. Two structural genes, pydA and pydB, encoding a metacleavage dioxygenase and a hydrolase, respectively, are required for degradation of HP, and three genes, pydC, pydD, and pydE, encoding proteins of an ABC transporter, are involved in the uptake of HP by TAL1145. These genes are induced by HP, although both pydA and pydB show low levels of expression without HP. pydA and pydB are cotranscribed, while pydC, pydD, and pydE are each transcribed from separate promoters. PydA and PydB show no homology with other dioxygenases and hydrolases in Sinorhizobium meliloti, Mesorhizobium loti, and Bradyrhizobium japonicum. Among various root nodule bacteria, the ability to degrade mimosine or HP is unique to some Leucaena-nodulating Rhizobium strains.

STUDY OF SOYBEAN SEED MICROFLORA AS А ONE OF PROBABLE FACTORS OF BRADYRHIZOBIUM JAPONICUM DISTRIBUTION

10.35868/1997-3004.6.84-91 ◽

2008 ◽

Vol 6 ◽

pp. 84-91

Author(s):

D.V. Krutуlo

Keyword(s):

Soybean Seed ◽

Seed Storage ◽

Soybean Seeds ◽

Nodule Bacteria ◽

Soybean Rhizobia ◽

Soybean Nodule ◽

Deep Layers

The results of studying of the soybean nodule bacteria presence in epiphytic and endophytic microflora of different grades of soybean seeds are presented. The soybean rhizobia was not revealed on the surface and in deep layers of soybean seeds. The soybean seed microbiota of investigated varieties is presented by several morphological types of bacteria and fungy. Their quantity depends on the period of seed storage.

THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE IN THE MIXED CULTURE IN VITRO

10.35868/1997-3004.13.70-81 ◽

2011 ◽

Vol 13 ◽

pp. 70-81

Author(s):

S.F. Kozar ◽

T.O. Usmanova ◽

T.A. Zherebor

Keyword(s):

Azospirillum Brasilense ◽

Stress Factors ◽

Lag Phase ◽

Bacterial Cells ◽

Nodule Bacteria ◽

Initial Concentration ◽

Soybean Nodule ◽

Growth Activity

As a result of the conducted researches the substantial influence of azospirillium bacteria on the growth activity of nodule bacteria as comparing to the influence of Bradyrhizobium japonicum on growth of Azospirillum brasilense was found out. The greatest quantity of the studied bacteria was observed at average initial concentration (0,272 milliard/cm3) of bacterial cells of every kind at their initial ratio 1:1. It was shown that combined cultivation of soybean nodule bacteria and azospirillium bacteria reduces effect of stress factors on the growth of B. japonicum in the lag-phase.

THE EFFICIENCy OF DIFFERENT MEANS OF SOyBEAN INOCULATION wITH NODULE BACTERIA

10.35868/1997-3004.10.20-28 ◽

2010 ◽

Vol 10 ◽

pp. 20-28

Author(s):

S.M. Malichenko ◽

V.K. Datsenko ◽

P.M. Mamenko ◽

S.Ya. Kots

Keyword(s):

Nitrogenase Activity ◽

Soybean Seeds ◽

Nodule Bacteria ◽

Standard Strain ◽

Bacterial Cultures ◽

Tn5 Mutants ◽

Soybean Plants ◽

Production Strain

The efficiency of the presowing soybean inoculation with nodule bacteria at direct seeds bacterization or introduction of inoculum to the soil at presowing cultivation as well as the ability of rhizobia remained in the soil to the next year to form active symbioses with soybean plants was studied. The liquid bacterial cultures of production strain Bradyrhizobium japonicum 634b and three perspective Tn5-mutants of B. japonicum 646 were used. The introduction of the inoculum to soil was shown to be more efficient during both years of investigations as compared with the seeds inoculation which resulted in higher number of nodules formed, their nitrogenase activity and greater soybean seeds yield. Two of three studied Tn5-mutants had surpassed the standard strain by the efficiency indices.

Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology ◽

АНАТОМІЧНА БУДОВА РОСЛИН GLYCINE MAX MOENCH. ЗА ДІЇ ШТАМУ BRADYRHIZOBIUM JAPONICUM ТА РЕТАРДАНТУ

10.25128//2078-2357.20.3-4.13 ◽

2020 ◽

Vol 80 (3-4) ◽

pp. 98-104

Author(s):

L. A. Golunova

Keyword(s):

Growth Regulators ◽

Leaf Surface ◽

Mutual Effect ◽

Crop Productivity ◽

Reproductive Organ ◽

Nodule Bacteria ◽

Vegetative Organs ◽

Chlorophyll Accumulation ◽

Leaf Surface Area

The effect of nodule bacteria strain Bradyrhizobium japonicum and the integrated use of the strain and retardant of the triazole class - tebuconazole on linear growth, anatomical structure of Glycine plants and their influence on crop productivity were studied in the field experiment. It was found that both the aplication of Bradyrhizobium japonicum M 8 strain solely and the complex use of the preparations (treatment of the plants with retardant at the beginning of reproductive organ formation, against presowing inoculation of soybean seeds) affected experimental plants’ height, histological parameters of the vegetative organs in comparison with those of untreated control. The use of growth regulators caused changes in the leaf surface area, mesostructural organization of the leaves and accumulation of photosynthetic pigments in them. There were also indicated observable changes in the size of the bark and mechanical tissues of the stem. The effect of the restrictive growth regulators was apparant in the following: the strain of nodule bacteria caused an increase in the linear size of the stem and leaf surface of plants against control. The mutual use of tebuconazole- retardant and strain Bradyrhizobium japonicum led to a decrease in apical dominance of plants, leaf thickness due to increased cell size of wall and spongy parenchymas and intensification of chlorophyll accumulation. Treated plants were characterized by changes in the size of their bark and structural tissues of the stem (collenchyma and sclerenchyma), which is a prerequisite for higher resistance to lodging of plants. The mutual effect of Bradyrhizobium japonicum strain and the triazole preparation proved to be most efficacious among the preparations used. Exogenous application of tebuconazole against the strain of nodule- forming bacteria contributes essentially to the strength of the stem, improves donor functions of Glycine hispida Max, which further secures growth and development of generative organs towards improving yield capasity.

FUNCTIONING OF SYMBIOTIC SYSTEMS OF COWPEA – NODULE BACTERIA

10.35868/1997-3004.12.46-58 ◽

2011 ◽

Vol 12 ◽

pp. 46-58

Author(s):

D.V. Krutуlo

Keyword(s):

High Specificity ◽

Positive Influence ◽

Nodule Bacteria ◽

Soil Culture ◽

Nitrogen Fixation Activity ◽

Bradyrhizobium Sp ◽

Slow Growing ◽

Cowpea Rhizobia ◽

Significant Positive Influence

The nodule bacteria were isolated from the nodules of cowpea. On the basis of phenotypical properties these rhizobia were referred to slow growing bacteria of Bradyrhizobium genus. Interaction features of cowpea with the nodule bacteria of cowpea (Bradyrhizobium sp. (Vigna)) and soybean (Bradyrhizobium japonicum) on nitrogen-free substrate and in soil culture were studied. It was established that the cowpea rhizobia strains possess high specificity to the host plant, promote symbiotic nitrogen fixation activity in 1,8-2,6 times and increase plants aboveground mass yield in 1,4-3,4 times, in comparison with control. The significant positive influence of the active soybean microsymbiont Bradyrhizobium japonicum 46 on the growth and development of cowpea was shown.

VIABILITY AND EFFICIENCY OF BRADYRHIZOBIUM JAPONICUM UNDER VARIOUS TECHNOLOGIES OF BACTERIAL PREPARATION PRODUCTION

10.35868/1997-3004.4.39-50 ◽

2007 ◽

Vol 4 ◽

pp. 39-50

Author(s):

S.Ya. Kots ◽

D.M. Sytnikov ◽

S.M. Malichenko ◽

N.A. Vorobey

Keyword(s):

Nodule Bacteria ◽

Bacterial Preparation ◽

Soybean Nodule ◽

Bacterial Preparations

Viability of soybean nodule bacteria under various technologies of production of bacterial preparations and influence of homologous lectin on their efficiency had been studied. It is shown that perlite and vermiculite are equivalent carriers in terms of technology for product¬ion of bacterial preparations. The findings indicate perspectiveness of homologous lectin ме as a component of bacterial preparations.

THE INFLUENCE OF ACTIVE STRAINS OF NODULE BACTERIA ON EFFICIENCY OF LEGUMINOUS PLANTS

10.35868/1997-3004.4.51-61 ◽

2007 ◽

Vol 4 ◽

pp. 51-61

Author(s):

E.V. Nadkernichna ◽

T.M. Kovalevska ◽

D.V. Krutilo ◽

V.P. Gorban ◽

V.S. Vorobej

Keyword(s):

Rhizobium Leguminosarum ◽

Nodule Bacteria ◽

Leguminous Plants ◽

Soybean Nodule ◽

Bradyrhizobium Sp ◽

Bacterial Preparations

The results of efficiency studying of new active strains of lupine, peas and soybean nodule bacteria in field and manufacture experiments are given. It is shown, that strains Bradyrhizobium sp. (Lupinus) 5500/4, Rhizobium leguminosarum bv.viceae T2, Bradyrhizobium japonicum КНЮ provide stable increase of leguminous culture crop in comparison with standard strains and they can be used to bacterial preparations manufacture.

Determination of toxicity of various preparative forms of pesticidal fungicides for nodule bacteria inoculants

E3S Web of Conferences ◽

10.1051/e3sconf/202022404032 ◽

2020 ◽

Vol 224 ◽

pp. 04032

Author(s):

Y V Laktionov ◽

Y V Kosulnikov ◽

V V Yachno ◽

A P Kozhemyakov

Keyword(s):

Temperature Regime ◽

Active Substance ◽

Rhizobium Leguminosarum ◽

Holding Time ◽

Expert Group ◽

Nodule Bacteria ◽

Biological Products ◽

Soybean Nodule

The aim of our study was to determine the effect of fungicide formulation, brand of fungicide, its concentration in the solution, holding time and temperature regime of the solution on the number of survivors of nodule bacteria of soybean, lupine, peas and lentils in a solution. Bacterial suspensions of soybean nodule bacteria (Bradyrhizobium japonicum 634b), lupine (Bradyrhizobium lupini 367a), pea (Rhizobium leguminosarum 261b), and lentils (Rhizobium leguminosarum 712) were studied. Wetting powders Benomil (active substance benomil 500 g/kg, LLC “Soyuzagrohim”, Russia), Benorad (active substance benomil 500 g/kg, JSC “August”, Russia) and Fundazol (active substance benomil 500 g/l, LLC “Agro-Kemi”, Russia) and concentrates of suspension Maxim KS (active substance fludioxonil, 25 g/l; “Syngenta International AG”, Switzerland), Protect KS (active substance fludioxonil, 25 g/l; LLC “Agro Expert Group”, Russia), Protect Forte VSK (active substance fludioxonil, 40 g/l + flutriafol, 30 g/l; LLC “Agro Expert Group”, Russia) were studied as fungicidal disinfectants. Compatibility was determined after the preparation of tank solutions of biological products and fungicides, followed by an assessment of the percentage of surviving rhizobia depending on the brand of fungicide, its concentration (10 and 20%), the holding time of the solution (2, 4, 8 h) and the temperature regime of the solution (2-5, 16-18, 27 °C).