scholarly journals Studies on the Antimicrobial Activity and Brine Shrimp Toxicity of the Leaves Extract of Ageratum Conyzoides

2016 ◽  
Vol 29 (2) ◽  
pp. 98-103
Author(s):  
AFM Shahid Ud Daula ◽  
Rokeya Siddiqui ◽  
Md Masud Alam ◽  
Md Abul Hossain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Positive Control ◽  
Gram Negative Bacteria ◽  
Ageratum Conyzoides ◽  
Vincristine Sulphate ◽  
Soluble Fractions

The purpose of this study is to evaluate the antimicrobial and cytotoxic properties of the leaves extracts of Ageratum conyzoides L. Ageratum conyzoides has been utilized for the treatment of various ailments. The crude methanolic extract of Ageratum conyzoides, n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extracts were screened for their antimicrobial activity against a number of gram positive and gram negative bacteria by disk diffusion method. The crude extract and n-hexane soluble fraction of crude extract showed significant to moderate antimicrobial activity and chloroform, ethyl acetate soluble fraction of crude extract showed moderate to mild antibacterial activity against gram positive and gram negative bacteria. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, chloroform and ethyl acetate soluble fractions were found to be 14.89 mm - 19.40 mm, 14 mm - 19.40 mm, 10.66 mm - 13.50 mm and 9.50 mm - 13.26 mm respectively at a concentration of 30 mg/disc. Chloroform, n-hexane and ethyl acetate soluble fractions of methanolic extract of Ageratum conyzoides were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it can be well predicted that nhexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Ageratum conyzoides possess cytotoxic activity on shrimp nauplii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 245.42 mg/ml, 92.61 mg/ml and 6.35 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 98-103

scholarly journals The Antimicrobial activity and Brine Shrimp Lethality Bioassay of Leaf extracts of Stephania japonica (Akanadi)

2012 ◽  
Vol 28 (2) ◽  
pp. 52-56 ◽  
Author(s):  
Rahman M Minhajur ◽  
M Masud Alam ◽  
A F M Shahid Ud Daula ◽  
Mohammad Shahriar ◽  
Md Mizanur Rahman Moghal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Positive Control ◽  
Gram Negative Bacteria ◽  
Brine Shrimp Lethality ◽  
Vincristine Sulphate ◽  
Soluble Fractions

The purpose of the present study is to examine the antibacterial and cytotoxic properties of methanol extract of leaves of Stephania japonica. The crude methanolic extract of S. japonica, n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract were screened for their antimicrobial activity against a wide range of both Gram-positive and Gram-negative bacteria by disc diffusion method. The crude extract showed moderate and n-Hexane, chloroform soluble fraction of crude extract showed mild antibacterial activity against both Gram-positive and Gram-negative bacteria. Ethyl acetate soluble fraction of the extract showed significant antimicrobial activity against Salmonella typhi, Escherichia coli and Bacillus cereus. The zones of inhibition produced by the crude methanolic extract, n-hexane, chloroform and ethyl acetate soluble fractions were found to be 12.80-16.55 mm, 12.60 mm, 5-14.30 mm and 10-20.25 mm, respectively, at a concentration of 30 g/disc. Chloroform, n-hexane and ethyl acetate soluble fractions of methanolic extract of S. japonica were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay, it can be well predicted that chloroform and ethyl acetate soluble fractions of methanolic extract of S. japonica possess cytotoxic principles (with LC50 66.488 mg/ml and LC50 45.662 mg/ml, respectively) comparison with positive control vincristine sulphate (with LC50 0.839 mg/ml). But n-hexane soluble fractions of methanolic extract of S. japonica exhibited no lethality effect on shrimp nauplii. DOI: http://dx.doi.org/10.3329/bjm.v28i2.11816 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 52-56

scholarly journals Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

2015 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Rokeya Siddiqui ◽  
Md Masud Alam ◽  
Mohammad Ruhul Amin ◽  
AFM Shahid Ud Daula ◽  
MM Hossain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate ◽  
Soluble Fractions

Bangladesh possesses a rich flora of medicinal plant. Out of the estimated 5000 species of phanerogams and pteridophytes growing in this country, more than a thousand are regarded as having medicinal properties. The aim of this study was to evaluate the in vitro antimicrobial activity and brine shrimp lethality of the whole plant extracts of Spilanthes paniculata, a plant belonging to the family Asteraceae. The crude extract, n-Hexane, chloroform and ethyl acetate soluble fraction of crude extract showed significant to moderate antimicrobial activity against three Gram positive and three Gram negative microorganisms. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, choloform and ethyl acetate soluble fractions were found to be 14.89 mm-19.40 mm, 14 mm-19.40 mm, 10.66 mm-13.50 mm and 9.50 mm-13.26 mm, consecutively at a concentration of 30 µg/disc. N-Hexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Spilanthes paniculata were screened for antitumor properties using brine shrimp lethality bioassay. From the results of the brine shrimp lethality bioassay, it can be well predicted that n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract possess mild cytotoxicity on shrimp naupalii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 48.978 mg/ml, 92.61 mg/ml and 216.770 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22743 Stamford Journal of Microbiology, Vol.3(1) 2013: 1-5

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

scholarly journals Antioxidant Property, Cytotoxicity and Antimicrobial Activity of Lasia spinosa Leaves

2013 ◽  
Vol 13 (2) ◽  
pp. 215-218 ◽  
Author(s):  
Durajan Goshwami ◽  
Md. Mostafizur Rahman ◽  
Md. Abdul Muhit ◽  
Md. Saiful Islam ◽  
Mukhtar Anasri
Keyword(s):  
Antimicrobial Activity ◽  
Brine Shrimp ◽  
Plant Material ◽  
Methanolic Extract ◽  
Antimicrobial Property ◽  
Antioxidant Property ◽  
Ic50 Value ◽  
Vincristine Sulphate ◽  
Line Slope ◽  
Best Fit

This study was undertaken to evaluate antioxidant property, cytotoxicity and antimicrobial activity of Lasia spinosa leaves. Ethyl acetate soluble partitionate of the methanolic extract of the plant material showed significant antioxidant property using DPPH (1,1-diphenyl-2-pecrylhydrazyl) assay with IC50 value of 16.42 ?g/ml. In the brine shrimp lethality bioassay, LC50 values obtained from the best fit line slope were 0.544, 3.181, and 4.096 ?g/mL for standard (vincristine sulphate), dichloromethane and ethylacetate soluble partitionates of the methanolic extract respectively. The leaves extracts showed mild antimicrobial property. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 215-218 DOI: http://dx.doi.org/10.3126/njst.v13i2.7739

scholarly journals Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic screening of the Crude Extracts

2012 ◽  
Vol 60 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Monirul Islam ◽  
MD Al-Amin ◽  
M Mahboob Ali Siddiqi ◽  
Shakila Akter ◽  
Mohammad Majedul Haque ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Ethanol Extract ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Test Organisms ◽  
Soluble Fractions

Quercetin-3-O-beta-D-glucopyranoside was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Azadirachta indica (Family: Meliaceae). The crude extract of hexane, ethylacetate and butanol soluble fractions of this ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61 µg/ml.DOI: http://dx.doi.org/10.3329/dujs.v60i1.10328Dhaka Univ. J. Sci. 60(1): 11-14, 2012 (January)

Phytochemical Profiling, Antimicrobial Activity and Brine Shrimps Lethality of Croton zambesicus Seeds and Leaves Extracts

2017 ◽  
Vol 2 (01) ◽  
Author(s):  
Hiba A. Ali ◽  
Hassan B. Elamin ◽  
Awatif A. Fagier ◽  
Awmaima E. Ahmed ◽  
Abdelhalim A. Hamza
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Brine Shrimp Lethality Assay

It is a well known medicinal plant in Sudan with different traditional uses. To date there are no chemical or biological records reported on C. zambesicus seeds. Air dried ground seeds and leaves of C. zambesicus were extracted using 80% methanol. The methanolic extract was sequentially fractionated with petroleum ether, chloroform and ethyl acetate. The obtained extracts of C. zambesicus seeds leaves were screened antimicrobial activities against four standard bacteria (Bacillus subtitles, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and two fungi (Aspergillus niger and Candida albicans) using in vitro agar diffusion method. The cytotoxicity evaluation of the bioactive fractions was carried out in vitro using brine shrimp lethality assay. The MIC of the seeds crude extract was 12 mg/ml against B. subtitles, E. coli, and P.aeruginosa. After fractionation of the seeds methanolic extract the antibacterial activity was mainly accumulated in the ethyl acetate phase with an MIC of 50 mg/ml against Bacillus subtitles. The MIC of the leaves crude extract was 6.25 mg/ml against S. aureus, Escherichia coli, and Pseudomonas aeruginosa. After fractionation of the leaves methanolic extract the antibacterial activity was mainly accumulated in the chloroform phase with an MIC of 12.5 mg/ml against Bacillus subtitles. Terpenoids were mainly detected in the petroleum ether fraction in addition to four compounds which were suggested to be phenolics. Phenolic acids and flavonoids were detected in the ethyl acetate fraction giving typical blue, yellow and orange colours with NPR at 366 nm. Terpenoids and polyphenols, mainly flavonoids, detected in C. zambesicus active extracts were believed to be responsible of the antimicrobial and antioxidant activities mentioned above. All extracts were quite safe in brine shrimp lethality assay. These results justify the traditional use of C. zambesicus seeds and leaves.

scholarly journals Steroids from the stem of Nymphaea stellata

2013 ◽  
Vol 37 (1) ◽  
pp. 109-113 ◽  
Author(s):  
Badrun Naher Chowdhury ◽  
Md Mozammel Haque ◽  
Md Hossain Sohrab ◽  
Farhana Afroz ◽  
MA Al-Mansur ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Cytotoxic Effect ◽  
Cytotoxic Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Wide Range ◽  
Bacteria And Fungi ◽  
Academy Of Sciences

Three steroids, namely 24-ethyl-5?-cholestan-3-one (1), 5?-stigmast-22-en-3-one (2), stigmast-5, 22-dien-3-one (3) have been isolated from N. stellata. The phytochemical and antimicrobial as well as cytotoxic activities of Nymphaea stellata were investigated in this study. Crude extracts of N. stellata and various column fractions exhibited poor antimicrobial activity against a wide range of Gram-positive and Gram-negative bacteria and fungi. The crude extract and the fractions showed significant cytotoxic effect when subjected to brine shrimp lethality bioassay. DOI: http://dx.doi.org/10.3329/jbas.v37i1.15687 Journal of Bangladesh Academy of Sciences, Vol. 37, No. 1, 109-113, 2013

scholarly journals In Vitro Cytotoxic and Anthelmintic Activities of Leonurus sibiricus L.

2012 ◽  
Vol 4 (3) ◽  
pp. 721-727 ◽  
Author(s):  
M. R. Saha ◽  
F.M.S.N. Ul Bari ◽  
M. A. Rahman ◽  
M. A. Islam
Keyword(s):  
Ethyl Acetate ◽  
Positive Control ◽  
Cytotoxic Activities ◽  
Methanolic Extracts ◽  
Vincristine Sulphate ◽  
The Family ◽  
Leonurus Sibiricus ◽  
Leaves And Roots ◽  
Soluble Fractions

This study investigated the cytotoxic and anthelmintic activities of Leonurus sibiricus L. (commonly known as Raktodrone in Bangladesh) belonging to the family Labiatae. The dried leaves and roots of L. sibiricus were extracted with methanol and fractionated by modified Kupchan method. The crude methanolic extracts as well as its soluble fractions of petroleum ether, ethyl acetate and chloroform were screened for cytotoxic activity using brine shrimp lethality bioassay. They were found to possess significant cytotoxic activities. The LC50 values of crude extract of leaves and its pet-ether, ethyl acetate and chloroform soluble fractions were 1.0, 2.0, 2.11 and1.33 µg/ml, respectively. On the other hand, the LC50 of crude methanolic extract of roots and fractions of pet-ether, ethyl acetate and chloroform were 2.0µg/ml, 2.81 µg/ml, 3.55 µg/ml and 7.58µg/ml, respectively. Vincristine sulphate was used as positive control. The crude methanol extract of leaves and roots also showed very good anthelmintic activities as determined against the earthworms, Pheretima posthuma. The study confirms the moderate anthelmintic and potent cytotoxic activities of leaf and root extracts of L. sibiricus.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i3.9998 J. Sci. Res. 4 (3), 721-727 (2012)

scholarly journals Evaluation of antioxidant activity and cytotoxic potential of Cryp-tocoryne ciliata

2013 ◽  
Vol 2 (2) ◽  
pp. 38-41
Author(s):  
Ummey Jannatun Nahar ◽  
Shah Marzia Mahjabin Lina
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Cytotoxic Activities ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate

Cryptocoryne ciliata belonging to the Araceae family has been investigated for isolation of its secondary metabolites and evaluation of biological activities of the crude extractives with special emphasis to the antioxidant activity and brine shrimp lethality bioassay. The whole plant was extracted with methanol and concentrated extract was partitioned using petroleum ether, carbon tetrachloride and ethyl acetate. Aqueous soluble fraction of the methanolic extract showed the highest antioxidant activity. The carbon tetrachloride soluble fraction of the methanolic extract and the ethyl acetate soluble fraction of the methanolic extract showed moderate antioxidant activity as compared to free antioxidant activity of tert-butyl-1-hydroxytolunene. In the brine shrimp lethality bioassay, among all extracts of whole plant of C. ciliata, the carbon tetrachloride soluble fraction of the methanolic extract showed strong cytotoxic activity. Aqueous soluble fraction of the methanolic extract, methanolic crude and ethyl acetate soluble fraction of the methanolic extract showed mild cytotoxity as compared to that of vincristine sulphate. The study confirms the mild to moderate antioxidant and moderate potent cytotoxic activities of C. ciliata plant extract as compared to reference standards and therefore demands the isolation of active principles and thorough bioassay.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13196 International Current Pharmaceutical Journal 2013, 2(2): 38-41

scholarly journals Neoandrographolide Isolated from Leaves of Adhatoda vasica Nees

2012 ◽  
Vol 60 (1) ◽  
pp. 1-3 ◽  
Author(s):  
MD Al-Amin ◽  
Md Monirul Islam ◽  
M Mahboob Ali Siddiqi ◽  
Shakila Akter ◽  
Shamim Ahmed ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Ethanol Extract ◽  
Adhatoda Vasica ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Test Organisms ◽  
Soluble Fractions

Neoandrographolide was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Adhatoda vasica (Family: Acanthaceae). The crude extracts of hexane, ethylacetate and butanol soluble fractions of ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61 µg/ml.DOI: http://dx.doi.org/10.3329/dujs.v60i1.10326Dhaka Univ. J. Sci. 60(1): 1-3, 2012 (January)

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