scholarly journals Effect of heavy metals on the readaptive processes in the urinary bladder

2018 ◽  
Vol 18 (1) ◽  
pp. 100-106
Author(s):  
Anatolii Romaniuk ◽  
Vladyslav Sikora ◽  
Yulia Lуndіna ◽  
Mykola Lуndіn ◽  
Natalia Hyriavenko ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Vitamin E ◽  
Urinary Bladder ◽  
Medical Science ◽  
Adaptation Period ◽  
Experimental Conditions ◽  
Laboratory Rats ◽  
Negative Effect ◽  
High Concentrations

Introduction: Heavy metals (HM) are dangerous elements due to their toxicity and prevalence play an important role in the environmental pollution and hazards to health risk. Impact of HM at high concentrations may lead to the failures of urinary bladder (UB) structure and functions. The aim of our study was to determine the readaptive changes in the UB of the rats and effectiveness of vitamin E using after the long-term intake of the HMS mixtures. Materials and Methods: We used mature male laboratory rats which were divided into three series with special experimental conditions after the influence of HM. The detailed analysis of the UB state was performed by using morphometric, spectrophotometric, histological and electron microscopy methods. Results: We had determined that the long-term intake of HM caused the significant increase of their accumulation in the tissue and morphometric changes of UB. Obtained results after the cancelation of the HM effect demonstrate smooth morphological restructuring of the UB wall with the reduction of difference of accumulation and morphometric indicators with control. The adjustment of vitamin E during there adaptation period showed better result recovery and optimization of all parameters, but they haven’t completely returned to normal state. Conclusions: The influence of HMS mixture led to significant negative effect on the UB at all stages of research. However, under the conditions of readaptation the tendency towards a certain stabilization of all indicators was observed, but they didn’t completely pass to the end of the study. The vitamin E using has caused the acceleration and improved the development of readaptive change after HM exposure. Bangladesh Journal of Medical Science Vol.18(1) 2019 p.100-106

scholarly journals VARIABILITY OF T-LYMPHOCYTES AND MACROFAGS COMPOSITION IN THE URINARY BLADDER UNDER THE INFLUENCE OF HEAVY METALS SALTS, CORRECTION OF THEIR EFFECT WITH VITAMIN E AND IN THE READAPTATION PERIOD

2018 ◽  
Vol 24 (1) ◽  
pp. 11-15
Author(s):  
V.V. Sikora
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
T Cells ◽  
Vitamin E ◽  
Urinary Bladder ◽  
Metal Salts ◽  
Male Rats ◽  
Quantitative Composition ◽  
Experimental Conditions ◽  
Heavy Metal Salts

In modern urology, it is important to study and understand the features of the development of various pathologies in the organs of the urinary system since the disturbances of the urodynamics is a widespread problem all over the world. Thus, the environment pollution caused by heavy metal salts has unpredictable consequences for the functioning of the urinary bladder. That is why the object of this study is to determine the features of the expression and the quantitative composition of T-cells and macrophages in the UB wall under the intake of the HMS and correction of their effect with vitamin E, as well as during the readaptation period. According to the experimental scenario, the experiment model was implemented by using male rats that were divided into six groups. The heavy metal salts mixture consisted of the most common potentially dangerous metals-microelements (zinc, cooper, iron, manganese, lead and chrome). Vitamin E was chosen as a corrector. To determine the quantitative composition of T-cells and macrophages the immunohistochemical identification by CD3 and CD68 antibodies was used. The main results are presented as M±SD, with the assessment of the differences and dependencies between the groups by using nonparametric Mann-Whitney U test and Spearman’s Rank Correlation Coefficient. We found that the number of CD3+ lymphocytes and CD68+ macrophages differed between the groups and depended on the experimental conditions. Thus, the results of our study demonstrate that the number of T-cells and macrophages in the bladder increases under the influence of heavy metals salts. The simultaneous intake of the pollutants and vitamin E is accompanied by the decrease of expression intensity of the corresponding cells. When the intake of the heavy metals is canceled, the qualitative composition of the identified CD3 and CD68 cells gradually comes to the norm, but the speed of its reduction differs depending on the group of the readaptation. Discovered that the decrease of T-cells and macrophages in the groups with the use of vitamin E was more approached to the control. Based on the results of the immunohistochemical study, a strong correlation between an increased number of the expressed T-cells and macrophages was revealed in the urinary bladder during the whole period of the study that demonstrates the dependence of their activity.

Temporal evolution and spatial distribution of heavy metals in a stormwater infiltration basin – estimation of the mass of trapped pollutants

2007 ◽  
Vol 56 (12) ◽  
pp. 93-100 ◽  
Author(s):  
S. Le Coustumer ◽  
P. Moura ◽  
S. Barraud ◽  
B. Clozel ◽  
J.-C. Varnier
Keyword(s):  
Heavy Metals ◽  
Urban Areas ◽  
Total Mass ◽  
Temporal Evolution ◽  
Infiltration Basin ◽  
Major Interest ◽  
High Concentrations ◽  
Stormwater Infiltration ◽  
Stormwater Infiltration Basin

Infiltration systems are widely used to manage stormwater in urban areas but questions about their long term sustainability need to be addressed. The possible pollution of the groundwater and the concentration of pollutant trapped in the soil are of major interest. This article focuses on the concentration of heavy metals found in the top layer of an infiltration basin. Two sampling campaigns have been undertaken 10 months apart and 200 samples were collected and analysed for lead (Pb), zinc (Zn) and copper (Cu). High concentrations were measured at the surface of the system; pollutant build up over time is clearly evident, especially in the lower lying areas, as well as higher concentrations in the oldest part of the system. Finally, the total mass of heavy metals trapped in the top layer of the systems are evaluated.

ACCUMULATION OF HEAVY METALS (CU, ZN, NI, CR, PB, CD) IN TISSUES OF PERCH (PERCA FLUVIATILIS L.) AND ROACH RUTILUS RUTILUS (L.) UNDER EXPERIMENTAL CONDITIONS/SUNKIŲJŲ METALŲ (CU, ZN, NI, CR, PB, CD) KAUPIMASIS EŠERIO PERCA FLUVIATILIS L. IR KUOJOS RUTILUS АККУМУЛЯЦИЯ ТЯЖЕЛЫХ МЕТАЛЛОВ (CU, ZN, NI, CR, PB, CD) В ТКАНЯХ ОКУНЯ PERCA FLUVIATILIS L. И ПЛОТВЫ RUTILLUS RUTILLUS L. В ЭКСПЕРИМЕНТАЛЬНЫХ УСЛОВИЯХ

2008 ◽  
Vol 16 (4) ◽  
pp. 205-212 ◽  
Author(s):  
Raimondas Leopoldas Idzelis ◽  
Vytautas Kesminas ◽  
Gintaras Svecevičius ◽  
Vaidas Misius
Keyword(s):  
Heavy Metals ◽  
Good Condition ◽  
Perca Fluviatilis ◽  
Rutilus Rutilus ◽  
Ecological Monitoring ◽  
Point Of View ◽  
Experimental Conditions ◽  
Roach Rutilus Rutilus ◽  
Perch Perca Fluviatilis

The paper analyses the effects of heavy metals on freshwater fishes. From the point of view of pollution caused by heavy metals, the condition of ecosystems in Lithuania is yet less affected by heavy metals compared to that the world over. Fishes accumulate heavy metals selectively. Fishes mostly accumulate Pb, Cr, Cd. Our experiments showed that MPC (Maximum Permitted Concentration) (0.2 mg/kg) of lead was exceeded 1.6 time in the muscles of perch, and 1.4 time ‐ in the muscles of roach. The MPC (0.05 mg/kg) of cadmium was exceeded 1.2 time in the muscles of perch and roach. It was determined that perch accumulate heavy metals more intensively than roach. Heavy metals accumulate in fishes: liver > muscle> gills. As heavy metals are considered to be one of the most harmful water pollutants, there is a great need for a constant ecological monitoring and reduction of their amounts released into the environment. According to long‐term research data, the MPC in fishes is exceeded only slightly. This confirms a good condition of aquatic ecosystems in Lithuania. Santrauka Įvertintas sunkiųjų metalų pasiskirstymas gėlavandenių žuvų audiniuose monitoringiniuose vandens telkiniuose, taip pat ir kuojų bei ešerių, paveiktų sunkiųjų metalų didžiausiomis leidžiamosiomis koncentracijomis (DLK), audiniuose. Eksperimentiniai tyrimai atlikti su Cu, Zn, Ni, Cr, Pb, Cd, kurių kiekvienas pasižymi kancerogeniniu mutageniniu poveikiu. Nustatyta, kad švino DLK (0,2 mg/kg) buvo viršyta ešerių raumenyse 1,6 karto, o kuojų – 1,4 karto. Kadmio DLK (0,05 mg/kg) ešerių ir kuojų raumenyse buvo viršyta apie 1,2 karto. Taip pat nustatyta, kad ešeriai sunkiuosius metalus kaupia intensyviau nei kuojos. Eksperimentiniai duomenys sutampa su sunkiųjų metalų tyrimų monitoringiniuose vandens telkiniuose duomenimis. Žuvų audiniuose Pb ir Cd koncentracijos yra didžiausios ir dažnai viršija Lietuvos higienos normoje nurodytąją DLK. Tokie rezultatai įpareigoja nuolat kontroliuoti sunkiųjų metalų kiekį žuvyse. Rezultatai pateikiami sunkiųjų metalų sklaidos pasiskirstymo grafikuose. Резюме Представлены данные по распределению тяжелых металлов в тканях пресноводных рыб в мониторинговых водоемах, а также результаты, полученные путем воздействия тяжелых металлов в предельно допустимых концентрациях (ПДК) на окуня и плотву. В экспериментальных исследованиях применялись металлы Cu, Zn, Ni, Cr, Pb, Cd, оказывающие на живые организмы воздействие мутагенного и канцерогенного характера. Установлено, что концентрация Cd в тканях окуня превышала ПДК (0,2 мг/кг) в 1,6 раза, а в тканях плотвы – в 1,4 раза. Концентрация Cd в тканях тех же рыб превышала ПДК (0,05 мг/кг) примерно в 1,2 раза. Также установлено, что окунь аккумулирует тяжелые металлы интенсивнее плотвы. Экспериментальные данные совпадают с данными, полученными в мониторинговых водоемах. Установлено также, что концентрация Pb и Cd в тканях рыб зачастую превышает ПДК. Полученные результаты обязывают постоянно контролировать уровень тяжелых металлов в тканях рыб.

scholarly journals Heavy metals in soils of the train removal station Ussuriysk JSC Russian Railways

2018 ◽  
Vol 7 (1) ◽  
pp. 54-57
Author(s):  
Denis Alexandrovich Klyuchnikov ◽  
Svetlana Aleksandrovna Platonova ◽  
Elena Vasilievna Soboleva
Keyword(s):  
Heavy Metals ◽  
Soil Samples ◽  
Pollution Level ◽  
Railway Transport ◽  
Sources Of Pollution ◽  
Technogenic Anomalies ◽  
High Concentrations ◽  
The City ◽  
Heavy Metals In Soils

The paper studies heavy metals in soils of the train removal station Ussuriysk JSC Russian Railways. An adverse effect of railway transport on adjacent territories is shown. Railway transport and subjects of its support are permanent sources of pollution. Long-term emissions to the air are reflected by essential accumulation of tocsins in the top horizons of the soil. The authors reveal the amount of heavy metals in soils of the train removal station Ussuriysk JSC Russian Railways. To identify the coefficient of heavy metals concentration in soil samples of 8 trial areas of technogenic and anthropogenic zones of the city were analyzed. To identify local technogenic anomalies concentration coefficients were calculated. The authors concluded that the territory of the train removal station Ussuriysk JSC Russian Railways was very polluted. The south of the railway station is characterized by an average pollution level. The authors revealed high concentrations of Zn, Mn, Fe, Cu and Pb in soils of the trial sites with a high transport loading.

scholarly journals Effects of Long-Term Selection in the Border Collie Dog Breed: Inbreeding Purge of Canine Hip and Elbow Dysplasia

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1743
Author(s):  
Virág Ács ◽  
György Kövér ◽  
János Farkas ◽  
Árpád Bokor ◽  
István Nagy
Keyword(s):  
Simulation Method ◽  
Positive Trend ◽  
Pedigree Data ◽  
Effective Population ◽  
Experimental Conditions ◽  
Term Selection ◽  
Inbreeding Coefficients ◽  
Elbow Dysplasia ◽  
Negative Effect

Pedigree data of 13,339 border collie dog was collected along with canine hip dysplasia (CHD) and canine elbow dysplasia (CED) records (1352 CHD and 524 CED), and an inbreeding–purging (IP) model was created. Ancestral inbreeding coefficients were calculated by using a gene dropping simulation method with GRain 2.2 software. Cumulative logit models (CLM) for CHD and CED were fitted using a logit-link Poisson distribution and the classical (F_W), and ancestral inbreeding (F_BAL, F_KAL, and F_KAL_NEW) coefficients as linear regression coefficients. The effective population size was calculated from F_W and decreased in the examined period along with an increase of F_W; however, slight differences were found as a consequence of breeding dog imports. CHD values were lowered by the expansion of F_BAL, as the alleles had been inbred in the past. For CHD, signs of purging were obtained. There was a positive trend regarding the breeding activity (both sire and dam of the future litters should be screened and certified free from CHD and CED), as years of selection increased the frequency of alleles with favorable hip and elbow conformation. Division of the ancestral inbreeding coefficient showed that alleles that had been identical by descent (IBD) for the first time (F_KAL_NEW) had a negative effect on both traits, while F_KAL has shown favorable results for alleles IBD in past generations. Some authors had proven this phenomenon in captive populations or experimental conditions; however, no evidence of inbreeding purge has ever been described in dog populations. Despite the various breeding practices, it seems that alleles of these polygenic disorders could be successfully purged out of the population with long-term selection.

scholarly journals The study of the protective properties of vitamin E on bone marrow of rats in condition of the increased intake of heavy metals salts

2019 ◽  
Vol 23 (1) ◽  
pp. 59-63
Author(s):  
Y. Lyndina ◽  
V. Sikora ◽  
N. Hyriavenko ◽  
М. Lyndin ◽  
A. Romaniuk
Keyword(s):  
Heavy Metals ◽  
Bone Marrow ◽  
Vitamin E ◽  
Male Rats ◽  
Hematopoietic Tissue ◽  
Protective Properties ◽  
Qualitative And Quantitative ◽  
Quantitative Characteristics ◽  
Negative Effect ◽  
Qualitative And Quantitative Characteristics

Homeostasis imbalance leads to the changes in qualitative and quantitative characteristics of all hematopoietic sprouts. It encourages to search for the effective protective means in the conditions of the polluted environment. Our research is focused on the study the protective properties of vitamin E on the bone marrow of the rats in conditions of the increased intake of the heavy metals salts. During the research the bone marrow of the 36 laboratory male rats was studied. The sections of the decalcified femoral bones were stained using hematoxylin and eosin method, Van Gieson’s technique and PAS-reaction, as well as immunohistochemical study to CD3, CD79α, S100 and MРО was used. Elemental composition of hematopoietic tissue was determined through atomic spectrophotometry. The accumulation of the heavy metals led to microelementosis (changes in qualitative and quantitative characteristics of all hematopoietic sprouts and surrounding tissues). The extension of the influence of the exogenous pollutants led to the suppression of erythro-and leucopoiesis, synchronous increase in the number of megakaryocytes. These indicators reached the significant deviations on the 90 day of the observation. The use of the vitamin E as a corrector reduces the negative effect of the pollutants that is demonstrated by the reduced accumulation of the heavy metals in the bone marrow, prevention of the significant deviations in the quantitative and qualitative composition of hematopoietic tissue.

READAPTIVE CHANGES IN THE URINARY BLADDER AFTER THE CANCELLATION OF THE HEAVY METALS SALTS INTAKE AND UNDER THE CORRECTION WITH THE VITAMIN E

2018 ◽  
Vol 14 (64) ◽  
pp. 169
Author(s):  
A. N. Romaniuk ◽  
V. V. Sikora ◽  
M. S. Lyndin ◽  
V. V. Sikora ◽  
D. R. Hyryavenko
Keyword(s):  
Heavy Metals ◽  
Vitamin E ◽  
Urinary Bladder

Effect of verapamil on cytoplasmic distribution of granules and microfilaments in amphibian urinary bladder

1988 ◽  
Vol 46 ◽  
pp. 324-325
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Morphological Changes ◽  
Granular Cell ◽  
Cytosolic Calcium ◽  
Calcium Storage ◽  
Amphibian Urinary Bladder ◽  
Vesicular Membrane ◽  
High Concentrations ◽  
Cytoskeletal System

The amphibian urinary bladder has been used as a ‘model’ system for studies of the mechanism of action of antidiuretic hormone (ADH) in stimulating transepithelial water flow. The increase in water permeability is accompanied by morphological changes that include the stimulation of apical microvilli, mobilization of microtubules and microfilaments and vesicular membrane fusion events . It has been shown that alterations in the cytosolic calcium concentrations can inhibit ADH transmembrane water flow and induce alterations in the epithelial cell cytomorphology, including the cytoskeletal system . Recently, the subapical granules of the granular cell in the amphibian urinary bladder have been shown to contain high concentrations of calcium, and it was suggested that these cytoplasmic constituents may act as calcium storage sites for intracellular calcium homeostasis. The present study utilizes the calcium antagonist, verapamil, to examine the effect of calcium deprivation on the cytomorphological features of epithelial cells from amphibian urinary bladder, with particular emphasis on subapical granule and microfilament distribution.

Infrastructure mortgage in Russia: Opportunities and prospects

2019 ◽  
pp. 114-133
Author(s):  
G. I. Idrisov ◽  
Y. Yu. Ponomarev
Keyword(s):  
Federal Government ◽  
Public Debt ◽  
Budget Deficit ◽  
Expert Assessment ◽  
Inflation Expectations ◽  
Direct Impact ◽  
Long Run ◽  
Gdp Growth Rate ◽  
Negative Effect

The article shows that depending on the goals pursued by the federal government and the available interbudgetary tools a different design of infrastructure mortgage is preferable. Three variants of such mortgage in Russia are proposed, each of which is better suited for certain types of projects and uses different forms of subsidies. According to our expert assessment the active use of infrastructure mortgage in Russia can increase the average annual GDP growth rate by 0.5 p. p. on the horizon of 5—7 years. In the long run the growth of infrastructure financing through the use of infrastructure mortgage could increase long-term economic growth by 0.9 p. p., which in 20—30 years can add 20—30% of GDP to the economy. However, the change in the structure of budget expenditures in the absence of an increase in the budget deficit and public debt will cause no direct impact on monetary policy. The increase in the deficit and the build-up of public debt will have a negative effect on inflation expectations, which will require monetary tightening for a longer time to stabilize them.

The depressing (negative) effect of oestradiol benzoate on the in vitro secretion of LH and FSH by the pituitary gland of the LRH-pretreated long-term ovariectomized rat changes into the augmentative (positive) effect after discontinuation of the LRH-pretreatment

1985 ◽  
Vol 110 (3) ◽  
pp. 329-337 ◽  
Author(s):  
G. A. Schuiling ◽  
H. Moes ◽  
T. R. Koiter
Keyword(s):  
Depressing Effect ◽  
Ovx Rats ◽  
Gonadotrophin Secretion ◽  
Oestradiol Benzoate ◽  
Negative Effect ◽  
Positive Effect ◽  
Perifusion System

Abstract. The effect of pretreatment in vivo with oestradiol benzoate on in vitro secretion of LH and FSH was studied in long-term ovariectomized (OVX) rats both at the end of a 5-day continuous in vivo pretreatment with LRH and 4-days after cessation of such LRH pretreatment. Rats were on day 0 sc implanted with osmotic minipumps which released LRH at the rate of 250 ng/h. Control rats were implanted with a piece of silicone elastomer with the dimensions of a minipump. On days 2 and 4 the rats were injected with either 3 μg EB or with oil. On day 5 part of the rats were decapitated and the in vitro autonomous (i.e. non-LRH-stimulated) and 'supra-maximally' LRHstimulated release of LH and FSH was studied using a perifusion system. From other rats the minipumps were removed on day 5 and perifusion was performed on day 9. On the 5th day of the in vivo LRH pretreatment the pituitary LH/FSH stores were partially depleted; the pituitaries of the EB-treated rats more so than those of the oil-injected rats. EB alone had no significant effect on the content of the pituitary LH- and FSH stores. On day 9, i.e. 4 days after removal of the minipumps, the pituitary LH and FSH contents had increased in both the oil- and the EB injected rats, but had not yet recovered to control values. In rats not subjected to the 5-days pretreatment with LRH EB had a positive effect on the supra-maximally LRH-stimulated secretion of LH and FSH as well as on the non-stimulated secretion of LH. EB had no effect on the non-stimulated secretion of FSH. After 5 days of in vivo pretreatment with LRH only, the in vitro non-stimulated and supra-maximally LRH-stimulated secretion of both LH and FSH were strongly impaired, the effect correlating well with the LRH-induced depletion of the pituitary LH/FSH stores. In such LRH-pretreated rats EB had on day 5 a negative effect on the (already depressed) LRH-stimulated secretion of LH (not on that of FSH). EB had no effect on the non-stimulated LH/FSH secretion. It could be demonstrated that the negative effect of the combined LRH/EB pretreatment was mainly due to the depressing effect of this treatment on the pituitary LH and FSH stores: the effect of oestradiol on the pituitary LRH-responsiveness (release as related to pituitary gonadotrophin content) remained positive. In LRH-pretreated rats, however, this positive effect of EB was smaller than in rats not pretreated with LRH. Four days after removal of the minipumps there was again a positive effect of EB on the LRH-stimulated secretion of LH and FSH as well as on the non-stimulated secretion of LH. The positive effect of EB on the pituitary LRH-responsiveness was as strong as in rats which had not been exposed to exogenous LRH. The non-stimulated secretion of FSH was again not affected by EB. The results demonstrate that the effect of EB on the oestrogen-sensitive components of gonadotrophin secretion consists of two components: an effect on the pituitary LRH-responsiveness proper, and an effect on the pituitary LH/FSH stores. The magnitude of the effect of EB on the LRH-responsiveness is LRH dependent: it is very weak (almost zero) in LRH-pretreated rats, but strong in rats not exposed to LRH as well as in rats of which the LRH-pretreatment was stopped 4 days previously. Similarly, the effect of EB on the pituitary LH and FSH stores is LRH-dependent: in the absence of LRH, EB has no influence on the contents of these stores, but EB can potentiate the depleting effect of LRH on the LH/FSH-stores. Also this effect disappear after cessation of the LRH-pretreatment.

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