scholarly journals Evaluation of antileishmanial activity of plants used in Indian traditional medicine

2015 ◽  
Vol 10 (2) ◽  
pp. 423
Author(s):  
Arushdeep Sidana ◽  
Umar Farooq
Keyword(s):  
Cell Death ◽  
Natural Products ◽  
Traditional Medicine ◽  
Plant Extracts ◽  
Antileishmanial Activity ◽  
Acorus Calamus ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Mtt Method

<p>The present study was aimed at <em>in vitro</em> antileishmanial screening of ten plants used in the traditional medicine in India. MTT method was used to evaluate the cell death after application of 100, 250, 350 and 500 μg/mL of the methanolic extracts followed by incubation for 24 hours at 25°C. Methanolic leaf extracts of <em>Acorus calamus, Alstonia scholaris</em> and <em>Berberis aristata</em> showed significant antileishmanial activity at a concentration of 500 µg/ml. In order to identify the antileishmanial compounds present in the active extracts of the screened plants, an LC-MS analysis of the tested extracts was carried out. The active extracts revealed the presence of some natural products with known antileishmanial activity along with other compounds. The present study suggests that the active plant extracts may be processed to isolate the compounds that may further be screened for their antileishmanial potential.</p>

scholarly journals Evaluation of Antileishmanial Activity of Albaha Medicinal Plants againstLeishmania amazonensis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Saeed S. Al-Sokari ◽  
Nasser A. Awadh Ali ◽  
Lianet Monzote ◽  
Mohamed A. Al-Fatimi
Keyword(s):  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Plant Extracts ◽  
Peritoneal Macrophages ◽  
Antileishmanial Activity ◽  
The Other ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Achillea Biebersteinii ◽  
Antileishmanial Activities

Sixteen methanolic extracts obtained from thirteen plant species, selected either from ethnobotanical or chemotaxonomical data, were screened for their antileishmanial activity againstLeishmania amazonensis. The cytotoxic activity against normal peritoneal macrophages from normal BALB/c mice was also determined. Eight extracts had IC50values ranging from <12.5 to 37.8 µg/mL against promastigotes.Achillea biebersteiniiflower,Euphorbia helioscopia,andSolanum incanumleaf extracts showed antileishmanial activities with IC50between <12.5–26.9 µg/mL and acceptable selectivity indices of 8–5. The other leishmanicidal plant extracts, with IC50ranging from 18.0 to 29.5 µg/mL, exhibited low selectivity indices.

scholarly journals Vasorelaxant Alkaloids from Spirospermum penduliflorum (Menispermaceae), a Plant Used to Treat Hypertension in Malagasy Traditional Medicine

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800
Author(s):  
Guy E. Raoelison ◽  
Mamy H. Rafamantanana ◽  
René Razafindrazaka ◽  
Adolphe Randriantsoa ◽  
Suzanne Urverg-Ratsimamanga ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Eastern Coast ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antagonist Activity ◽  
Methanolic Extracts ◽  
Bioassay Guided Fractionation ◽  
Vasorelaxant Activity ◽  
The Right

Spirospermum penduliflorum Thouars (Menispermaceae) is widely used on the eastern coast of Madagascar to treat hypertension. The aim of the present study was to analyse the vasorelaxant properties of different leaf extracts. The activity of the n-hexane, dichloromethane and methanolic extracts was tested on phenylephrine-contracted aorta. The dichloromethane extract was shown to be the most effective. Further fractionation of this extract led to the isolation of an active fraction relaxing phenylephrine-contracted aorta with an IC50 of 0.18 μg/mL {log IC50 (μg/mL) −0.74 ± 0.03} but was much less effective on KCl induced contractions. Bioassay-guided fractionation of this fraction led to the isolation of two aporphinoid alkaloids, neolitsine and dicentrine, which at concentrations of 0.1 μM and 1 μM displaced to the right the phenylephrine concentration-contraction curve. Our results show that Spirospermum penduliflorum extracts possess vasorelaxant activity in vitro that could be related to the presence of dicentrine in the extracts having an α1 antagonist activity. This finding is not in accord with the previous studies by Rasoanaivo et al where no alkaloids were detected in the leaves of Spirospermum penduliflorum.

scholarly journals The Antiviral Effect of Indonesian Medicinal Plant Extracts Against Dengue Virus In Vitro and In Silico

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 85 ◽  
Author(s):  
Rosmalena Rosmalena ◽  
Berna Elya ◽  
Beti E. Dewi ◽  
Fithriyah Fithriyah ◽  
Hidayati Desti ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Plant Extracts ◽  
In Silico ◽  
In Silico Analysis ◽  
Acorus Calamus ◽  
Methanolic Extracts ◽  
Free Energy Of Binding ◽  
Dose Dependent ◽  
Silico Analysis

Dengue infections are still a worldwide burden, especially in Indonesia. There is no specific medication against the dengue virus. Recently, many types of research have been conducted to discover a new drug for dengue virus using natural resource extracts. Indonesia, as a tropical country, has a wide biodiversity. There are several medicinal plants in Indonesia that are believed to possess anti-dengue activity, such as Myristica fatua, Cymbopogon citratus, and Acorus calamus plants. We conducted an in vitro laboratory experiment of several extracts from Indonesian herbs combined with in silico analysis. The extracts were evaluated for safety and antiviral activity in Huh7it-1 cell lines, using a single dose of 20 µg/mL and dose-dependent (5, 10, 20, 40, 80 and 160 µg/mL) of plant extracts against dengue virus serotype 2 (DENV-2) NGC strain. The DMSO 0.1% was used as a negative control. The cytotoxic aspect was assessed by counting the cell viability, while the antiviral activity was calculated by counting the average inhibition. The selectivity index (SI) of plant extracts were performed from a ratio of CC50/EC50 value. In silico analysis was conducted to determine the free energy of binding between NS5 of dengue virus with bioactive compounds contained in Myristica fatua, Cymbopogon citratus and Acorus calamus extract plants. We determined that all extracts were not toxic against Huh7it-1 cell lines. The methanolic extracts of A. calamus, C. citratus, and M. fatua showed inhibition of DENV-2 at a dose of 20 µg/mL to 96.5%, 98.9%, and 122.7%, respectively. The dose-dependent effects showed that M. fatua has the best inhibition activity towards DENV-2. Molecular docking result showed that artesunic acid within M. fatua has the best free energy of binding (−7.2 kcal/mol), followed by homoegonol (−7.1 kcal/mol) which was slightly different from artesunic acid among others. The methanolic extracts of A. calamus, C. citratus, and M. fatua showed prospective anti-dengue activities both in vitro and in silico. Future research should be conducted to find the pure extracts of all useful herbs as a new candidate of antiviral drug.

Oreganum Vulgare: In-vitro assessment of cytotoxicity, Molecular docking studies, Antioxidant, and evaluation of anti-inflammatory activity in LPS stimulated RAW 264.7 cells

2020 ◽  
Vol 16 ◽  
Author(s):  
Reyaz Hassan Mir ◽  
Gifty Sawhney ◽  
Rohini Verma ◽  
Bilal Ahmad ◽  
Parveen Kumar ◽  
...  
Keyword(s):  
Natural Products ◽  
Traditional Medicine ◽  
Dynamic Network ◽  
Docking Studies ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Leaf Extracts ◽  
Anti Inflammatory ◽  
Isolated Compound

Background: Inflammation involves a dynamic network that is highly regulated by signals that initiate the inflammation process as well as signals that downregulate it. However, an imbalance between the two leads to tissue damage. Throughout the world, inflammatory disease becomes common in the aging society. The drugs which are used clinically suffer serious side effects. Natural products or compounds derived from natural products show diversity in structure and play an important role in drug discovery and development. Objective: Oreganum Vulgare is used in traditional medicine for various ailments including respiratory and rheumatic disorders, severe cold, suppression of tumors. The current study aims to evaluate the anti-inflammatory potential by evaluating various in-vitro parameters. Results: The extracts (OVEE, OVEAF) as well as the isolated compound(OVRA)of Oreganum Vulgare inhibit the proinflammatory cytokines (IL-6 and TNF-α) and NO without affecting cell viability. Conclusion: Our study established that the leaf extracts of Oreganum Vulgare exhibits anti-inflammatory activity and thus confirm its importance in traditional medicine.

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals The Effects of Apigenin and Curcumin on Autophagy Related Cell Death and Apoptosis

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1586
Author(s):  
Sera Kayacan ◽  
Kaan Yilancioglu ◽  
Ayse Seda Akdemir ◽  
Fatma Kaya Dagistanli ◽  
Gulay Melikoglu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Death ◽  
Natural Products ◽  
Plant Extracts ◽  
Mtt Assay ◽  
Hela Cells ◽  
Qrt Pcr ◽  
Antiviral Effects ◽  
Related Cell ◽  
Promising Therapeutic Approach

: Cervical cancer is one of the frequent types of cancer seen in females. It has been suggested that natural compounds can be used effectively for cancer treatment. Apoptosis and autophagy related cell death play important roles in suppression of tumorigenesis. Apigenin and curcumin are natural products isolated from plant extracts known to have antitumoral, antibacterial and antiviral effects. Varying doses of curcumin and apigenin were applied to HeLa cancer cell lines. The expression of the genes related to apoptosis and/or autophagy related cell death were measured using qRT-PCR and cell viability was measured using MTT assay. Our results showed that curcumin and apigenin are effective on apoptosis and autophagy related cell death in HeLa cells. We suggested that these natural products seem to be a new promising therapeutic approach in cancer.

scholarly journals In Vitro Antibacterial and Antifungal Activity and Datura Innoxia Extracts

2014 ◽  
Vol 3 (3) ◽  
pp. 173-185
Author(s):  
Adam IY Shama ◽  
YM Abd-Kreem ◽  
AA Fadowa ◽  
RM Samar ◽  
MK Sabahelkhier
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Antibacterial Drug ◽  
Aqueous Extracts ◽  
Datura Innoxia ◽  
Methanolic Extracts ◽  
Leaves And Roots

The aim of this study was evaluated the Antimicrobial Activity of extraction of Datura innoxia (Seeds, leaves and roots). Datura innoxia Seeds, leaves and roots were collected to examine their antimicrobial activity. Extracts of different parts of the plant were tested against standard microorganisms by using the agar- well diffusion method. Extracts of methanol, and aqueous of seeds, leaves and roots were prepared and tested against four types of bacteria namely: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris and two types of fungi namely: Aspergillus niger and Candida albicans. The methanolic and aqueous extracts of leaves showed high activities against fungi (A. niger) and less effect on the all bacteria. The methanolic extracts of seeds showed high activities against all organisms except fungi (C. albicanas), while the aqueous extracts of seeds showed no activity on the bacteria. All organisms were examined against known standard antibiotics and then compare the results of plant extracts with standard antibiotics. The results indicated that the antibacterial drug is less active than the plant extracts, while the antifungal drugs are more active than the plant extracts. DOI: http://dx.doi.org/10.3126/ije.v3i3.11077 International Journal of Environment Vol.3(3) 2014: 173-185

scholarly journals Evaluation of Plant Extracts against Stemphylium Blight of Lentil

2016 ◽  
Vol 16 (1) ◽  
pp. 11-16
Author(s):  
S. Subedi ◽  
S. M. Shrestha ◽  
G. B. KC ◽  
R. B. Thapa ◽  
S. K. Ghimire ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Water Extract ◽  
Experimental Results ◽  
Grain Legume ◽  
Acorus Calamus ◽  
Short Period ◽  
Stemphylium Blight ◽  
Unsprayed Plot ◽  
Treated Plot

Experiments were carried out to evaluate five different plant extracts against lentil stemphylium blight (Stemphylium botryosum Walr.) at the Grain Legume Research Program, Rampur, Chitwan, Nepal during two winter seasons (2011/ 12 and 2012/13) using CRD in laboratory and RCBD in field conditions. Water extract of Acorus calamus at higher dose (8% W/V) was able to check the growth of the pathogen completely in-vitro. The mycelial growth inhibition (%) of Xanthoxylum armatum and Lantana camera at 8% concentration in PDA were 31.17 and 24.56 respectively. In the field experiment during 2011/12, extracts were sparingly effective for a short period. However the disease control (PDC) was higher in X. armatum treated plot (31.60%) followed by A. calamaus (28.69%) and L. camera (27.61%) when compared to unsprayed plot. The following year (2012/13) field experimental results also verified the effect of plant extracts against the disease. The lower Disease (%) Index (PDI) was obtained from the plots treated with A. calamus (35.33%) and X. armatum (35.55%). Higher crop yield was recorded from the plot treated with A. calamus (987.39 kg/ha) followed by X. armatum (972.78 kg/ha). Two years’ experimental results revealed that A. calamus and X. armatum were effective botanicals to manage lentil Stemphylium blight.Nepal Journal of Science and Technology Vol. 16, No.1 (2015)  pp. 11-16

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