scholarly journals Mouse model of ulcerative colitis using trinitrobenzene sulfonic acid

2015 ◽  
Vol 10 (4) ◽  
pp. 860
Author(s):  
Irfan Ahmad Rather ◽  
Vivek K. Bajpai ◽  
Nam Gyeong-Jun
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Mouse Model ◽  
Bowel Disease ◽  
Sulfonic Acid ◽  
Intestinal Inflammation ◽  
Cost Effective ◽  
Trinitrobenzene Sulfonic Acid ◽  
Clinical Presentations ◽  
Inflammatory Bowel

<p>Animal model of intestinal inflammation is of paramount significance that aids in discerning the pathologies underlying ulcerative colitis and Crohn’s disease, the two clinical presentations of inflammatory bowel disease. The 2,4,6-trinitrobenzene sulfonic acid (TNBS) colitis model represents one such intestinal inflammation-prototype that is generated in susceptible strains of mice through intra-rectal instillation of compound TNBS. In this paper, we demonstrate the experimental induction of TNBS-mediated colitis in a susceptible strain of ICR mice. This can be done by the following steps: a) acclimation, b) induction and c) observation. TNBS-mouse model provides the information in shortest possible time and simultaneously represents a cost effective and highly reproducible model method of studying the pathogenesis of inflammatory bowel disease.</p><p><strong>Video Clips</strong></p><p><a href="https://youtube.com/v/6MsuIGzH3uA">Acclimation and induction of TNBS</a>:          4.5 min</p><p><a href="https://youtube.com/v/ya66SNwoVag">Observation and drug administration</a>:      1.5 min</p>

Physiological Basis for Novel Drug Therapies Used to Treat the Inflammatory Bowel Diseases I. Immunology and therapeutic potential of antiadhesion molecule therapy in inflammatory bowel disease

2005 ◽  
Vol 288 (2) ◽  
pp. G169-G174 ◽  
Author(s):  
Gert Van Assche ◽  
Paul Rutgeerts
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Adhesion Molecules ◽  
Bowel Disease ◽  
Intestinal Inflammation ◽  
Therapeutic Potential ◽  
Physiological Basis ◽  
Inflammatory Bowel

Adhesion molecules regulate the influx of leukocytes in normal and inflamed gut. They are also involved in local lymphocyte stimulation and antigen presentation within the intestinal mucosa. In intestinal inflammation, many adhesion molecules are upregulated, but α4-integrins most likely hold a key position in directing leukocytes into the inflamed bowel wall. Therapeutic compounds directed against trafficking of leukocytes have been designed and are being developed as a novel class of drugs in the treatment of Crohn's disease and ulcerative colitis. This review deals with the immunological aspects of leukocyte trafficking focused on gut homing of T cells. Second, the changes in adhesion molecules and T cell trafficking during intestinal inflammation are discussed. Finally, we review the clinical data that have been gathered with respect to the therapeutic potential and the safety of antiadhesion molecule treatment. Antegren, or natalizumab, a humanized anti-α4 integrin IgG4 antibody, has been most extensively evaluated and may be close to registration. A more specific humanized α4β7-integrin MLN-02 has shown preliminary clinical efficacy in ulcerative colitis, and both antergren and MLN-02 appear to be very safe. Trials with the anti-ICAM-1 antisense oligonucleotide ISIS-2302 in steroid refractory Crohn's disease have provided conflicting efficacy data. In the near future, some of these novel biological agents may prove valuable therapeutic tools in the management of refractory inflammatory bowel disease, although it is too early to define the patient population that will benefit most from these agents.

scholarly journals Recent Advances: The Imbalance of Cytokines in the Pathogenesis of Inflammatory Bowel Disease

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Qingdong Guan ◽  
Jiguo Zhang
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Inflammatory Cytokines ◽  
Bowel Disease ◽  
Intestinal Inflammation ◽  
Beneficial Effects ◽  
Recent Advances ◽  
Inflammatory Bowel ◽  
Inflammation Targeting ◽  
Anti Inflammatory

Cytokines play an important role in the immunopathogenesis of inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, where they drive and regulate multiple aspects of intestinal inflammation. The imbalance between proinflammatory and anti-inflammatory cytokines that occurs in IBD results in disease progression and tissue damage and limits the resolution of inflammation. Targeting cytokines have been novel strategies in the treatment of IBD. Recent studies show the beneficial effects of anticytokine treatments to IBD patients, and multiple novel cytokines are found to be involved in the pathogenesis of IBD. In this review, we will discuss the recent advances of novel biologics in clinics and clinical trials, and novel proinflammatory and anti-inflammatory cytokines found in IBD with focusing on IL-12 family and IL-1 family members as well as their relevance to the potential therapy of IBD.

Granulocytes Infiltrate The Intestine In a Zebrafish Model Of Inflammatory Bowel Disease

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 2276-2276
Author(s):  
Brenda Geiger ◽  
Aileen W. Zhen ◽  
Efi Kokkotou ◽  
Paula G. Fraenkel
Keyword(s):  
Inflammatory Bowel Disease ◽  
Intestinal Mucosa ◽  
Bowel Disease ◽  
Intestinal Inflammation ◽  
Conflicts Of Interest ◽  
Post Treatment ◽  
Trinitrobenzene Sulfonic Acid ◽  
Zebrafish Model ◽  
Transcript Levels ◽  
Inflammatory Bowel

Abstract Inflammatory bowel disease (IBD) is a condition in which lymphocytes and neutrophils infiltrate the intestine, stimulate the production of various inflammatory cytokines, and injure the intestinal mucosa. Because of the conserved aspects of hematopoiesis in the zebrafish, we hypothesized that a zebrafish model of IBD would reproduce human features of the disease, while retaining desirable aspects of zebrafish disease models including small size, low cost, and ease of chemical and genetic screening. We used adult transgenic zebrafish expressing eGFP under control of a myeloperoxidase promoter (Tg(mpx:eGFP) to trace the activity of granulocytes following intrarectal injection with trinitrobenzene sulfonic acid (TNBS), 1.6 mmole/gram body weight, or an equivalent volume of vehicle alone (30% ethanol). The zebrafish were sacrificed 6 hours’ post-treatment for assessment of changes in intestinal architecture, intestinal infiltration by myeloperoxidase-positive leukocytes, and cytokine transcript levels using histology, flow cytometry, and quantitative realtime RT-PCR, respectively. At 6 hours post-treatment, we detected a mononuclear infiltrate in the intestinal mucosa and a 10-fold increase in the number of myeloperoxidase-positive leukocytes in the intestines of TNBS-treated fish vs controls (0.614%±0.202% vs. 0.052%±0.014%, p=0.0269; n=6-7 fish per group). We also observed a significant increase in the transcript levels of interleukin-1beta (6,935±2,454 AU vs 100±28 AU, p=0.0179), interleukin-8 (246 ± 63 AU vs 100 ± 21 AU, p=0.05) and interleukin-10 (385 ± 84 AU vs 100± 8 AU p=0.0055) in the intestines of fish injected with TNBS vs vehicle alone (n=9-10 fish per group). These findings resemble those seen in patients and mammalian chemical injury models of IBD. They will provide the basis for further study of the mechanisms controlling intestinal inflammation in IBD. Disclosures: No relevant conflicts of interest to declare.

Isoorientin Alleviates Inflammatory Bowel Disease by Inhibiting NLRP3 Inflammasome Activation through Nrf2/NQO1 Pathway

2020 ◽  
Vol 18 (4) ◽  
pp. 392-397
Author(s):  
Qirun Cheng ◽  
Xianjin Yu ◽  
Rong Zhang ◽  
Lipeng Chen
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Sulfonic Acid ◽  
Tissue Injury ◽  
Biological Activities ◽  
Trinitrobenzene Sulfonic Acid ◽  
Inflammasome Activation ◽  
Nucleotide Binding Domain ◽  
Nlrp3 Inflammasome Activation ◽  
Inflammatory Bowel

Inflammatory bowel disease comprises a series of related conditions characterized by idiopathic inflammation of the gastrointestinal tract. To develop therapeutic agents to combat these conditions, a better understanding of the inflammatory mechanisms is of paramount importance. Isoorientin is a c-glycosylflavone common to plants such as Phyllostachys japonicus and buckwheat. While it has been documented to exhibit multiple biological activities, its effects on inflammatory bowel disease, and the potential regulatory mechanism remain to be explored. We have shown here that isoorientin relieves the intestinal tissue injury and decreases the activity and expression of myeloperoxidase in trinitrobenzene sulfonic acid-exposed rats. Furthermore, isoorientin alleviated cytokine secretion in rats after trinitrobenzene sulfonic acid exposure. Also, isoorientin suppressed the levels of the nucleotide-binding domain and leucine-rich repeat-containing protein family and enhanced the Nrf2/NQO1 pathway in trinitrobenzene sulfonic acid-induced bowel disease. In conclusion, isoorientin could serve as a promising drug for the treatment of chronic enteritis.

Ulcerative colitis

2011 ◽  
Author(s):  
R. Mark Beattie ◽  
Anil Dhawan ◽  
John W.L. Puntis
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Family History ◽  
Bowel Disease ◽  
Clinical Course ◽  
Younger Child ◽  
Clinical Presentations ◽  
History Of ◽  
Inflammatory Bowel ◽  
Index Cases

Clinical presentations 310Investigation 311Clinical course 311Management 31225% of inflammatory bowel disease presents in childhood, 1/3 as ulcerative colitis. Presentation can occur at any age and ulcerative colitis is the commonest cause of inflammatory bowel disease in the younger child. Family history of Crohn's disease or ulcerative colitis is common in index cases....

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