Evaluation of a poly-herbal preparation for the treatment of peptic ulcer

<p class="Abstract">The aim of this study was to validate the traditional uses of ulcerene, a poly-herbal formulation in ethanol, aspirin and stress-induced gastric ulcer model of rat. The extent of gastric ulcer formation was studied, using ulcer score, ulcer index, percentage cure through gross examination and histopathological evaluation. A significant (p<0.001) dose-dependent anti-ulcerant effect was observed in ulcerene (50 and 100 mg/kg)-treated group with highest effectiveness against ethanol-induced ulcer. The concentration-dependent spasmolytic effect was seen in spontaneously contracting, high K<sup>+</sup> (80 mM) and carbachol (1 µM)-induced jejunum contractions (10, 0.3 and 1 mg/mL), similar to dicyclomine (10, 1 and 3 µM), indicated non-specific spasmolytic mechanism behind the effect. By considering these results, ulcerene can be suggested for the treatment of peptic ulcer.</p>

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Prevention of peptic ulcer by aqueous extract of Aegle marmelos leaf in rats

IMC Journal of Medical Science ◽

10.3329/imcjms.v12i1.35171 ◽

2018 ◽

Vol 12 (1) ◽

pp. 11-14

Author(s):

Sharmin Rahman ◽

Mohammad Rezaul Quader ◽

Md Ismail Khan

Keyword(s):

Body Weight ◽

Peptic Ulcer ◽

Gastric Ulcer ◽

Aqueous Extract ◽

Protective Efficacy ◽

Albino Rats ◽

Ulcer Formation ◽

Aegle Marmelos ◽

Rat Stomach ◽

Dose Dependent

Background and objectives: Aegle marmelos (Bael), a medicinal plant, has been widely used indigenously to treat many diseases in Bangladesh and other countries. The present study was carried out to evaluate the efficacy of A. marmelos leaf to prevent ethanol induced gastric ulcer in a rat model.Methods: Thirty two Wister albino rats of either sex, weighing between 100-150g, were fed 200 mg/kg or 400 mg/kg aqueous extract of A. marmelos leaves one hour prior to oral administration of 90% ethanol (1 ml/200 gm body weight) to induce gastric ulcer. The animals were sacrificed after one hour and ulcer scores and index were determined. The protective efficacy of A. marmelos aqueous extract was expressed as percentage protection of ulcer.Results: Aqueous extract exhibited significant (p<0.05) dose dependent protection against gastric ulcer formation by ethanol in rat stomach. Percentage protection of ulcer with 200 mg/kg and 400 mg/kg of aqueous extract of A. marmelos leave were 19.3% and 37.2% respectively compared to standard anti-peptic ulcer drug omeprazole (50.4%).Conclusion: Thus, crude extracts of A. marmelos leave have been shown to have potential ability to prevent experimentally induced peptic ulcer formation in animal model.IMC J Med Sci 2018; 12(1): 11-14

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GASTROPROTECTIVE EFFECT OF SESBANIA GRANDIFLORA LINN. SEEDS EXTRACT.

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v9i2.735 ◽

2020 ◽

Vol 9 (2) ◽

Author(s):

Pallavi. B ◽

Sunil. N ◽

Syed Mansoor Ahamed ◽

Nandheesh R.

Keyword(s):

Peptic Ulcer ◽

Gastric Ulcer ◽

Small Intestine ◽

World Population ◽

Total Acidity ◽

Peptic Ulcers ◽

Ulcer Index ◽

Standard Drug ◽

Sesbania Grandiflora ◽

Gastroprotective Effect

Background: A peptic ulcer is a sore in the lining of the stomach or the first part of the small intestine. Ulcer can be developed inside the inner lining of the stomach (gastric ulcer) or the small intestine (duodenal ulcer). Peptic ulcers are usually aggravated by an imbalance between destructive and defensive factors in the stomach, it affects nearly 10% of world population. The objective of the study was to investigate the protective effect of ethanolic extract of Sesbania grandiflora Linn. Seeds (SGEE) on peptic ulcer induced by ethanol in experimental rats. Objectives: Gastroprotective effect of Sesbania grandiflora Linn. Seeds extract on experimental Materials and Methods: The gastroprotective effect of SGEE was studied using ethanol (70% ) induced acute gastric ulcer models. The animals were divided into five groups, each group contains six animals. SGEE was administered in two doses, (200 mg/kg and 400 mg/kg, p.o). The parameters investigated include acid volume, pH, total acidity, ulcer index, total protein, glutathione, lipid peroxidase, catalase & histopathological studies. Results: 1. In gastroprotective effect study, SGEE significantly inhibited the development of ulcers induced by ethanol. The SGEE significantly reduced the acid volume, total acidity, total acidity, ulcer index, lipid peroxidation & increases in pH, glutathione & catalase level. Histopathogical studies also revealed that SGEE is gastro-protective. Ranitidine (100 mg/kg) is used as standard drug. Conclusion: All the observation implies that SGEE possess significant protective activity against ethanol induced gastric ulcer in experimental rats. 400 mg/kg doses has shown more protection compared to 200 mg/kg (dose dependent activity was obtained). Keywords: Antiulcer; Sesbania grandiflora; Ethanol; Ranitidine.

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EVALUATION OF GASTROPROTECTIVE EFFECT OF COENZYME Q 10 AND L - GLUTAMINE IN STRESS AND ETHANOL-INDUCED PEPTIC ULCER IN WISTAR RATS: A NOVEL PRECLINICAL STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.25904 ◽

2018 ◽

Vol 11 (9) ◽

pp. 365

Author(s):

Balaji Ommurugan ◽

Shalini Adiga ◽

Vijetha Shenoy Belle

Keyword(s):

Peptic Ulcer ◽

Coenzyme Q ◽

Antioxidant Property ◽

Preclinical Study ◽

Experimental Models ◽

Ulcer Formation ◽

Peptic Ulcers ◽

Ulcer Disease ◽

Ulcer Model ◽

Post Hoc

Objective: Coenzyme Q 10 (CoQ10) and L-glutamine have antioxidant property their role in peptic ulcer diseases is not well known. Hence, the aim of present study is to evaluate the gastroprotective role of CoQ10 and L-glutamine in stress and ethanol-induced peptic ulcers in Wistar albino rats.Methods: A total of 90 rats were used for the conduct of study in two experimental models, the stress- and the ethanol-induced ulcer model. Each model consists of eight groups with six rats in each group. Sucralfate and pantoprazole were used as standard drugs along with CoQ10 and L- glutamine as test drugs. Drugs were administered for 10 days in stress model and 7 days in case of ethanol model. Statistically analyzed using analysis of variance and post-hoc test with significance as p>0.05Results: CoQ10 and L- glutamine when used alone as well in combination with pantoprazole and sucralfate showed no ulcer formation. Test drugs showed decrease in gastric acid secretion, decreased total and free acidity levels, higher gastric pH, increased mucous secreting ability, higher levels of reduced glutathione levels in both tissue as well as blood and lower malondialdehyde levels when compared with pantoprazole and sucralfate suggestive of their antioxidative benefit, in both the models.Conclusion: Well-designed clinical trials can be done to evaluate the use of CoQ10 and L- glutamine in the treatment of peptic ulcer disease due to various etiology.

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Pharmacological and Phytochemical Evaluation Of Gastric Anti-Ulcer Activity of Parthenium hysterophorus In Different Models

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i4.8114 ◽

2017 ◽

Vol 9 (4) ◽

Author(s):

Ananda Kumar Chettupalli ◽

Vasudha B. ◽

Krishna Sanka

Keyword(s):

Gastric Ulcers ◽

Gastrointestinal Disorders ◽

Antiulcer Activity ◽

Peptic Ulcers ◽

Gastric Mucus ◽

Ulcer Index ◽

Traditional System ◽

Ulcer Model ◽

Dose Dependent ◽

Lesion Index

The cause of ulceration in patients is mainly due to hyper secretion of gastric juice and also due to hyper secretion of pepsin. In traditional system of medicine a number of herbal preparations have been used for the treatment of peptic ulcers. There are various medicinal plants has been used for the treatment of gastrointestinal disorders. In view of this, in present study we have to evaluate the anti-ulcer activity of parathenium hysterophorus. Study was carried out, by using three methods i.e., alcohol, paracetamol and stress induced ulcers in rats pretreated with the doses of 250 mg/kg AQPH and ALPH, 20mg/kg Omeoprazole and 50 mg/kg Ranitidine. To evaluate the antiulcer activity of aqueous and alcoholic extracts of parathenium hysterophorus leaves (AQPH and ALPH) at 250 doses using different experimentally induced gastric ulcer models in rats. Gastric ulcers were induced in rats by 80% alcohol, paracetamol and forced immersion stress induced methods. In alcohol induced ulcer model, paracetamol induced ulcer model and stress induced model the ulcer index was determined. Where as in stress induced ulcers stress plays an important role in ulcerogenesis. In alcohol-induced ulcers, AQPH and ALPH were effective in reducing lesion index and increasing the gastric mucus content. It was also effective in decreasing ulcer index in paracetamol-induced ulcers. All the results obtained with parathenium hysterophorus were dose dependent. The results suggest that AQPH and ALPH possesses significant and dose dependent antiulcer activity. The antiulcer activity of AQPH and ALPH can be attributed to its cytoprotective and antisecretory action.

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Evaluation of Antioxidant And Anti-Ulcerogenic Effects of Eremurus Persicus (Jaub & Spach) Boiss Leaf Hydroalcoholic Extract On Ethanol-Induced Gastric Ulcer In Rats

10.21203/rs.3.rs-653039/v1 ◽

2021 ◽

Author(s):

Mohammad Beiranvand ◽

Seifollah Bahramikia ◽

Omid Dezfoulian

Keyword(s):

Oxidative Stress ◽

Gastric Ulcer ◽

Plant Extract ◽

Gastric Ulcers ◽

Dependent Manner ◽

Ulcer Index ◽

Stress Injury ◽

Hydroalcoholic Extract ◽

Pre Treatment ◽

Dose Dependent

Abstract This study aimed to investigate the antioxidant and protective effect of Eremurus persicus (Jaub & Spach) leaf hydroalcoholic extract (EPE) in preventing gastric ulcers induced by ethanol in rats. Wistar rats weighing 180-220 g were randomly divided into five groups, included normal group, ethanol consumption group, ranitidine treatment experienced group, the recipient rats prepared by dose of 250 mg/kg plant extract, and the last group recipient the dose of 500 mg/kg plant extract. The gastric ulcer was induced by feeding 1 ml of 96% ethanol to each animal. The gastric ulcer index (UI), pH, oxidative stress parameters, and histopathological changes in all stomachs were measured. Pre-treatment of ethanol-induced rats with the EPE reduced (P<0.05) the ulcer index and gastric juice pH, compared to ethanol intake rats. Additionally, pre-treatment with EPE at a dose-dependent manner alleviated the gastric oxidative stress injury in rats by increasing CAT activity, tissue NO• and GSH levels. EPE also was able to decrease the levels of ROS, MDA, PCO and serum NO•. According to the results, it can be concluded that pre-treatment with EPE prevents the formation of gastric ulcers caused by ethanol, which can be attributed to the antioxidant activity of plant polyphenols compounds.

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