scholarly journals Membrane Stabilizing and Preliminary Hypoglycemic Activities of Picrasma javanica

2013 ◽  
Vol 16 (1) ◽  
pp. 89-92
Author(s):  
Tasnuva Sharmin ◽  
Farhana Islam ◽  
Md. Al Amin Sikder ◽  
Shaila Kabir ◽  
Mohammad Rashedul Haque ◽  
...  
Keyword(s):  
Body Weight ◽  
Salicylic Acid ◽  
Carbon Tetrachloride ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

The methanol extract of leaves of Picrasma javanica Blume and its n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were evaluated for membrane stabilizing and preliminary hypoglycemic activities. In hypotonic solution and heat-induced conditions, the crude methanol extract inhibited 65.30±0.52% and 36.79±0.38% haemolysis of RBC as compared to 72.79% and 42.12% produced by standard drug, acetyl salicylic acid, respectively (0.10 mg/ml). The crude extract also showed blood sugar lowering efficacy at 200 and 400 mg/ kg body weight, with 48.63% reduction of blood sugar level at 400 mg/kg body weight while the standard drug, Glibenclamide (10 mg/kg body weight) demonstrated 53.7% reduction of blood sugar level at 3rd hour of administration. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14500 Bangladesh Pharmaceutical Journal 16(1): 89-92, 2013

scholarly journals In vitro Thrombolytic and Membrane Stabilizing Studies of Brassica rapa subsp. chinensis (L.) Hanelt

2015 ◽  
Vol 17 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Nushrat Jahan ◽  
SM Ashikur Rahman ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brassica Rapa ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Negative Control ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

Brassica rapa subsp. chinensis (L.) Hanelt is a popular herb in Bangladesh. In the present studies, the plant has been assessed to evaluate its in vitro thrombolytic and membrane stabilizing properties. Initially, the plant was collected, processed and extracted with methanol and the concentrated crude methanol extract was fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble materials. All extractives, including the parent extract were subjected to thrombolytic and membrane stabilizing bioassays. In case of thrombolytic study, the carbon tetrachloride soluble fraction exhibited highest clot lysis. Here, streptokinase and distilled water were used as positive and negative control, respectively. However, in the assay for membrane stabilizing activity, the crude methanol extract along with other extractives were capable to inhibit hemolysis of erythrocyte membrane in hypotonic solution- and heat- induced conditions, which indicated anti-inflammatory property of the samples. Acetyl salicylic acid was used as standard drug in this assay. This represents the first report of thrombolytic and membrane stabilizing activities of B. rapa subsp. chinensis. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22336 Bangladesh Pharmaceutical Journal 17(2): 172-176, 2014

scholarly journals Membrane Stabilizing and Thrombolytic Activities of Sida rhombifolia L.

2015 ◽  
Vol 17 (1) ◽  
pp. 43-45
Author(s):  
Mohammd Firoz Khan ◽  
Sikder Nahidul Islam Rabbi ◽  
Md Aslam Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Salicylic Acid ◽  
Human Erythrocyte ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Thrombolytic Activity ◽  
Whole Plant ◽  
Crude Methanol Extract ◽  
Sida Rhombifolia

The crude methanol extract of whole plant of Sida rhombifolia and different fractions generated from it were tested for membrane stabilizing and thrombolytic activities. In hypotonic solution- and heat-induced conditions, the chloroform soluble partitionates inhibited haemolysis of human erythrocyte by 22.11±0.14% and 64.71±0.08%, respectively as compared to 71.97±0.51% and 40.12±0.29% demonstrated by the standard acetyl salicylic acid (ASA). Moreover, the pet ether soluble materials revealed highest thrombolytic activity with clot lysis value of 19.51±0.03% as compared to 66.98±0.11% exhibited by the standard streptokinase. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22313 Bangladesh Pharmaceutical Journal 17(1): 43-45, 2014

scholarly journals Analgesic, Antidiarrheal and Antidepressant Activities of Anethum sowa Linn. in Swiss-Albino Mice Model

2018 ◽  
Vol 21 (1) ◽  
pp. 1-6
Author(s):  
Muhammad Abdullah Al Mansur ◽  
M Mahboob Ali Siddiqi ◽  
Koushik Saha
Keyword(s):  
Body Weight ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Mice Model ◽  
Immersion Method ◽  
Standard Drug ◽  
Writhing Test ◽  
Tail Immersion ◽  
Anethum Sowa

The hexane (HE), dichloromethane (DCME), ethyl acetate (EAE) and methanol (ME) extracts of seed and stem of Anethum sowa were subjected to screenings for analgesic, anti-diarrheal and anti-depressant activities. The peripheral and central analgesic actions were determined by using formalin-induced writhing test and tail immersion method. The DCME extract of stem, at a dose of 200 mg/kg body weight, significantly reduced the number of writhing movements whereas the methanol extract of seed at the same dose exhibited remarkable analgesic activity in tail immersion method. In castor oil induced anti-diarrheal assay, the ethyl acetate extract of stem, at 400 mg/kg body weight, exhibited significant anti-diarrheal effect. In addition, significant anti-depressant activity was observed at a dose of 400 mg/kg body weight as compared to the standard drug in case of HE extract of stem.Bangladesh Pharmaceutical Journal 21(1): 1-6, 2018

scholarly journals In vitro Antioxidant, Membrane Stabilizing and Thrombolytic Activities of Glycosmis arborea

2012 ◽  
Vol 15 (2) ◽  
pp. 141-143 ◽  
Author(s):  
Sayed Mamur Hossain ◽  
Farhana Islam ◽  
Tasnuva Sharmin ◽  
Hasib Sheikh ◽  
AM Rashedul Hasan ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Rat Erythrocytes ◽  
Thrombolytic Activity ◽  
Negative Controls

The methanol extract of leaves and stems of Glycosmis arborea and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for antioxidant activity by Folin-Ciocalteau reagent, membrane stabilizing and thrombolytic activities. The carbon tetrachloride soluble fraction of stems of G. arborea demonstrated the presence of significant amount of phenolic compounds (36.95 ± 0.54 mg of GAE/g of extract). The extractives inhibited heat as well as hypotonic solution-induced haemolysis of rat erythrocytes in vitro. The pet-ether soluble fraction of leaves and stems of G. arborea showed 20.46 and 38.24% and 22.50 and 48.25%. inhibition of hemolysis of RBC caused by heat and hypotonic solution, as compared to 30.55 and 72.91% inhibition of hemolysis of RBC caused by the standard Acetyl salicylic acid at 0.01 mg/ml concentration, respectively. Among the four fractions, the chloroform soluble materials of the stems of G. arborea revealed highest thrombolytic activity with clot lysis value of 36.50% while standard streptokinase and water used as positive and negative controls, showed 64.25±0.26 and 2.35±0.35 % lysis of clot respectively DOI: http://dx.doi.org/10.3329/bpj.v15i2.12579 Bangladesh Pharmaceutical Journal 15(2): 141-143, 2012

scholarly journals In vitro Antioxidant, Cytotoxic and Membrane Stabilizing Activities of Bauhinia acuminata L.

2015 ◽  
Vol 17 (1) ◽  
pp. 99-101
Author(s):  
Mohammad Firoz Khan ◽  
Rabeya Islam Shilpi ◽  
Ridwan Bin Rashid ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Hypotonic Solution ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Bauhinia acuminata and its Kupchan fractions were screened for antioxidant, cytotoxic and membrane stabilizing activities. Among all partitionates the aqueous soluble fraction of B. acuminata demonstrated the highest antioxidant activity with IC50 value of 7.22±0.200 ?g/ml. Moreover, the carbon tetrachloride soluble fraction showed significant cytotoxic activity having LC50 value of 12.13±0.215 ?g/ml. On the other hand, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited haemolysis of human erythrocyte by 63.94±0.14% and 51.95±0.20%, respectively as compared to 81.97±0.77% and 42.11±0.39% demonstrated by the standard acetyl salicylic acid. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22324 Bangladesh Pharmaceutical Journal 17(1): 99-101, 2014

scholarly journals Bioactivities of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd.

2013 ◽  
Vol 16 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Farjana Chowdhury ◽  
Senjuti Pal ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
Md. Al Amin Sikder ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Lysis Activity ◽  
Crude Methanol Extract

The methanol extracts of leaf of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd. as well as their petroleum ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screenings for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin- Ciocalteau reagent using butylated hydroxytolune (BHT) and ascorbic acid as standards. The aqueous soluble fraction of the crude methanol extract of A. chaplasha and the methanolic crude extract of B. spectabillis demonstrated the highest free radical scavenging activity with the IC50 of 15.60±0.27 ?g/ml and 18.33±0.17 ?g/ml, which could be correlated to their total phenolic content of 61.26±0.23 and 50.00±0.19 mg of GAE/gm of extractive, respectively. Different extractives of A. chaplasha and B. spectabillis showed clot lysis activity ranging from 2.56±0.68% to 17.07±0.23% and 2.24±0.71% to 17.59±0.74% as compared to 66.77% and 3.791 % produced by the standard streptokinase and water, respectively. In hypotonic solution and heat induced conditions, the crude extract of A. chaplasha inhibited the haemolysis of human erythrocyte by 41.96±0.87% and 40.00±0.78%, while the carbon tetrachloride soluble fraction of methanol extract of B. spectabillis inhibited haemolysis by 65.68±0.36% and 35.62±0.82%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79% and 42.12% of haemolysis of RBCs in hypotonic and heat induced conditions, respectively. Among the extractives of both plants, the crude methanol extract of A. chaplasha and the chloroform soluble fraction of B. spectabillis extract revealed mild to moderate antimicrobial activity with zone of inhibition ranging from with 7.0 to 13.0 mm. The general toxicity was determined by brine shrimp lethality bioassay where the pet-ether soluble fraction of A. chaplasha (LC50 0.781±0.36 ?g/ml) and the aqueous soluble partitionate of B. spectabillis (LC50 1.28±0.57 ?g/ml) suggested the presence of considerable bioactive principles. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14493 Bangladesh Pharmaceutical Journal 16(1): 63-68, 2013

scholarly journals In vitro Membrane Stabilizing and In vivo Analgesic Activities of Boehmeria glomerulifera Miq. in Swiss-Albino Mice Model

2016 ◽  
Vol 19 (2) ◽  
pp. 185-189
Author(s):  
Sabikunnahar Papia ◽  
Muhammed Mahfuzur Rahman ◽  
Md Mustafezur Rahman ◽  
Mohaiminul Adib ◽  
Mohammad Firoz Khan
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Hypotonic Solution ◽  
Mice Model ◽  
Immersion Method ◽  
Tail Immersion ◽  
Crude Methanol Extract

Boehmeria glomerulifera Miq. is an indigenous plant of Bangladesh. Traditional healers use this plant to treat various diseases. The crude methanol extract of whole plant of B. glomerulifera and its Kupchan fractions were screened for in vitro membrane stabilizing and only the crude extract at doses of 200- and 400- mg/kg body weight were screened for analgesic activity in mice model in order to establish the medicinal values of this plant. The membrane stabilizing activity of the extractives was evaluated by the ability of test materials to inhibit hypotonic solution- and heat-induced haemolysis of human erythrocytes. Moreover, the analgesic activity of methanol extract was evaluated by acetic acid induced writhing method and tail immersion method in mice. In hypotonic solution- and heat-induced conditions, the crude methanol extract showed inhibition of haemolysis by 64.80±0.34% and 21.63±0.76%, respectively as compared to 77.74±0.67% and 40.41±0.69% demonstrated by the standard, acetyl salicylic acid. On the other hand, the analgesic activity was determined for its peripheral and central pharmacological responses using acetic acid-induced writhing test and tail immersion method at doses of 200- and 400- mg/kg body weight. The extract reduced the acetic acid-induced writhings significantly (p < 0.05) in a dose dependent manner with the highest activity observed at 400 mg/kg (46.0%) body weight when compared to that of Diclofenac sodium (64.0%), as the standard drug. A significant increase (p < 0.01) of latency period was also found in tail immersion method. Therefore, the present study established that the methanol extract of B. glomerulifera possesses moderate membrane stabilizing and significant analgesic activities.Bangladesh Pharmaceutical Journal 19(2): 185-189, 2016

scholarly journals In vitro and In vivo Investigations Provide New Insights into Bioactivities of Blumea clarkei Hook.f. Leaves

2021 ◽  
Vol 24 (2) ◽  
pp. 149-158
Author(s):  
Farzana Kabir ◽  
Asif Uj Jaman ◽  
Rizia Azad Rumpa ◽  
Tabassum Jannat ◽  
Safaet Alam ◽  
...  
Keyword(s):  
Body Weight ◽  
Crude Extract ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Brine Shrimp Lethality Assay ◽  
Crude Methanol Extract

As there is a resurgence of interest in plants as promising sources of new active pharmaceutical constituents, the present study has been designed to establish the preliminary biological activities of Blumea clarkei Hook.f. leaves. Here, the crude methanol extract of the leaves along with its organic and aqueous soluble fractions were subjected to different in vitro and in vivo assays. The ethyl acetate soluble fraction exhibited the highest total phenolic content (71.59 mg of GAE/gm of extract) and DPPH free radical scavenging (IC50 value 19.25 μg/ml) whereas another polar fraction; the aqueous soluble extractive showed the maximum cytotoxicity (LC50 value of 18.60 μg/ml) in the brine shrimp lethality assay. In membrane stabilizing activity evaluation, the inhibition of hypotonic solution and heat induced hemolysis were revealed maximum by the DCM (30.64%) and aqueous soluble fraction (39.15%), respectively while the crude methanol extract exhibited the highest thrombolytic potential (30.47% clot lysis). The central analgesic activity of the crude extract significantly increased the latent response time in tail-immersion method (p<0.001) at doses 200 and 400 mg/kg of body weight after 90 minutes of administration when compared to the control group. In acetic acid-induced writhing method, the plant extract showed prominent peripheral analgesia (p<0.001) with 47.54% and 65.57% inhibition at 200- and 400-mg/kg of body weight, respectively. In addition, a dose dependent hypoglycemic and antidiarrheal activities were also observed by the crude extract. Bangladesh Pharmaceutical Journal 24(2): 149-158, 2021

scholarly journals In vitro Cytotoxic, Membrane Stabilizing and Thrombolytic Activities of Polygonum glabrum Willd

2015 ◽  
Vol 17 (2) ◽  
pp. 202-204 ◽  
Author(s):  
Mohammad Firoz Khan ◽  
Sikder Nahidul Islam Rabbi ◽  
Fahima Aktar ◽  
Md Hassan Kawsar
Keyword(s):  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Activity Assay ◽  
Thrombolytic Activity ◽  
Lc50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Polygonum glabrum Willd and its Kupchan fractions were screened for cytotoxic, membrane stabilizing and thrombolytic activities. Among all fractions, the crude methanol extract showed significant cytotoxic activity having LC50 value 0.74 ± 0.045 ?g/ml. Moreover, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited hemolysis of human erythrocyte by 79.21 ± 0.44% and 84.87±0.23%, respectively as compared to 71.9 ± 0.73% and 42.12 ± 0.37% demonstrated by the standard acetyl salicylic acid. On the other hand, in thrombolytic activity assay the methanol extract demonstrated highest clot lysis value of 35.17 ± 0.42%. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22341 Bangladesh Pharmaceutical Journal 17(2): 202-204, 2014

scholarly journals Evaluation of Bioactivities of Gouania tiliaefolia Lam., an Indigenous Traditional Medicinal Plant of Bangladesh

2019 ◽  
Vol 22 (2) ◽  
pp. 200-207
Author(s):  
Tufael Ahmed ◽  
Rifat Khan ◽  
Nafisa Tabassum ◽  
Fahima Aktar ◽  
Mohammad Kaisarul Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Oral Glucose ◽  
Tail Flick ◽  
Cytotoxic Activities ◽  
Crude Methanol Extract

The crude methanol extract of Gouania tiliaefolia Lam. was partitioned by the modified Kupchan method and the fractions were evaluated for total phenolic content, antioxidant, cytotoxic, thrombolytic, hypotonic and heat-induced membrane stabilizing activities. The petroleum ether soluble fraction (PESF) and methanol extract (ME) showed the highest phenolic content of 78.30 ±1.60 mg and 70.37 ± 0.84 mg, respectively, which were expressed in gallic acid equivalent (GAE). Similar trends were observed in case of anti-oxidant and cytotoxic activities, where the PESF possessed the highest free radical scavenging activity and brine shrimp lethality (IC50 = 2.88 ± 0.02 μg/ml, LC50 = 2.59 ± 0.14 μg/ml), followed by ME (IC50 = 4.79 ± 0.17 μg/ml, LC50 = 3.38 ± 0.08 μg/ml) and CSF (IC50 = 37.51 ± 0.96 μg/ml, LC50 = 73.55 ± 0.26 μg/ml). In case of assays for thrombolytic and membrane stabilizing activities, all extractives showed insignificant results compared to the respective standards. The crude methanol extract of G. tiliaefolia was used to examine the in-vivo analgesic (central and peripheral), antidiarrheal and antidiabetic activities in Swiss albino mice. In case of castor oil induced diarrhea, the ME gave better reduction of diarrhea by 71.43% (at 400 mg/kg-body weight) compared to loperamide (64.29%). Antidiabetic activity was evaluated by oral glucose tolerance test and the ME showed 71.42% and 75.39% reduction of blood glucose at doses 200 and 400 mg/kg-body weight, respectively when compared with the standard glibenclamide that reduced blood glucose by 66.17%. The central- and peripheral-analgesic activity was evaluated by the tail-flick test and acetic acid induced writhing test, respectively. In both the cases, ME demonstrated dose-dependent analgesic activity compared to the standards. Bangladesh Pharmaceutical Journal 22(2): 200-207, 2019

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