scholarly journals In vitro Antioxidant, Cytotoxic and Membrane Stabilizing Activities of Bauhinia acuminata L.

2015 ◽  
Vol 17 (1) ◽  
pp. 99-101
Author(s):  
Mohammad Firoz Khan ◽  
Rabeya Islam Shilpi ◽  
Ridwan Bin Rashid ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Hypotonic Solution ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Bauhinia acuminata and its Kupchan fractions were screened for antioxidant, cytotoxic and membrane stabilizing activities. Among all partitionates the aqueous soluble fraction of B. acuminata demonstrated the highest antioxidant activity with IC50 value of 7.22±0.200 ?g/ml. Moreover, the carbon tetrachloride soluble fraction showed significant cytotoxic activity having LC50 value of 12.13±0.215 ?g/ml. On the other hand, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited haemolysis of human erythrocyte by 63.94±0.14% and 51.95±0.20%, respectively as compared to 81.97±0.77% and 42.11±0.39% demonstrated by the standard acetyl salicylic acid. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22324 Bangladesh Pharmaceutical Journal 17(1): 99-101, 2014

scholarly journals In vitro Cytotoxic, Membrane Stabilizing and Thrombolytic Activities of Polygonum glabrum Willd

2015 ◽  
Vol 17 (2) ◽  
pp. 202-204 ◽  
Author(s):  
Mohammad Firoz Khan ◽  
Sikder Nahidul Islam Rabbi ◽  
Fahima Aktar ◽  
Md Hassan Kawsar
Keyword(s):  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Activity Assay ◽  
Thrombolytic Activity ◽  
Lc50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Polygonum glabrum Willd and its Kupchan fractions were screened for cytotoxic, membrane stabilizing and thrombolytic activities. Among all fractions, the crude methanol extract showed significant cytotoxic activity having LC50 value 0.74 ± 0.045 ?g/ml. Moreover, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited hemolysis of human erythrocyte by 79.21 ± 0.44% and 84.87±0.23%, respectively as compared to 71.9 ± 0.73% and 42.12 ± 0.37% demonstrated by the standard acetyl salicylic acid. On the other hand, in thrombolytic activity assay the methanol extract demonstrated highest clot lysis value of 35.17 ± 0.42%. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22341 Bangladesh Pharmaceutical Journal 17(2): 202-204, 2014

scholarly journals In vitro Antioxidant, Membrane Stabilizing and Thrombolytic Activities of Glycosmis arborea

2012 ◽  
Vol 15 (2) ◽  
pp. 141-143 ◽  
Author(s):  
Sayed Mamur Hossain ◽  
Farhana Islam ◽  
Tasnuva Sharmin ◽  
Hasib Sheikh ◽  
AM Rashedul Hasan ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Rat Erythrocytes ◽  
Thrombolytic Activity ◽  
Negative Controls

The methanol extract of leaves and stems of Glycosmis arborea and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for antioxidant activity by Folin-Ciocalteau reagent, membrane stabilizing and thrombolytic activities. The carbon tetrachloride soluble fraction of stems of G. arborea demonstrated the presence of significant amount of phenolic compounds (36.95 ± 0.54 mg of GAE/g of extract). The extractives inhibited heat as well as hypotonic solution-induced haemolysis of rat erythrocytes in vitro. The pet-ether soluble fraction of leaves and stems of G. arborea showed 20.46 and 38.24% and 22.50 and 48.25%. inhibition of hemolysis of RBC caused by heat and hypotonic solution, as compared to 30.55 and 72.91% inhibition of hemolysis of RBC caused by the standard Acetyl salicylic acid at 0.01 mg/ml concentration, respectively. Among the four fractions, the chloroform soluble materials of the stems of G. arborea revealed highest thrombolytic activity with clot lysis value of 36.50% while standard streptokinase and water used as positive and negative controls, showed 64.25±0.26 and 2.35±0.35 % lysis of clot respectively DOI: http://dx.doi.org/10.3329/bpj.v15i2.12579 Bangladesh Pharmaceutical Journal 15(2): 141-143, 2012

scholarly journals In vitro Thrombolytic and Membrane Stabilizing Studies of Brassica rapa subsp. chinensis (L.) Hanelt

2015 ◽  
Vol 17 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Nushrat Jahan ◽  
SM Ashikur Rahman ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brassica Rapa ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Negative Control ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

Brassica rapa subsp. chinensis (L.) Hanelt is a popular herb in Bangladesh. In the present studies, the plant has been assessed to evaluate its in vitro thrombolytic and membrane stabilizing properties. Initially, the plant was collected, processed and extracted with methanol and the concentrated crude methanol extract was fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble materials. All extractives, including the parent extract were subjected to thrombolytic and membrane stabilizing bioassays. In case of thrombolytic study, the carbon tetrachloride soluble fraction exhibited highest clot lysis. Here, streptokinase and distilled water were used as positive and negative control, respectively. However, in the assay for membrane stabilizing activity, the crude methanol extract along with other extractives were capable to inhibit hemolysis of erythrocyte membrane in hypotonic solution- and heat- induced conditions, which indicated anti-inflammatory property of the samples. Acetyl salicylic acid was used as standard drug in this assay. This represents the first report of thrombolytic and membrane stabilizing activities of B. rapa subsp. chinensis. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22336 Bangladesh Pharmaceutical Journal 17(2): 172-176, 2014

scholarly journals In vitro and In vivo Investigations Provide New Insights into Bioactivities of Blumea clarkei Hook.f. Leaves

2021 ◽  
Vol 24 (2) ◽  
pp. 149-158
Author(s):  
Farzana Kabir ◽  
Asif Uj Jaman ◽  
Rizia Azad Rumpa ◽  
Tabassum Jannat ◽  
Safaet Alam ◽  
...  
Keyword(s):  
Body Weight ◽  
Crude Extract ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Brine Shrimp Lethality Assay ◽  
Crude Methanol Extract

As there is a resurgence of interest in plants as promising sources of new active pharmaceutical constituents, the present study has been designed to establish the preliminary biological activities of Blumea clarkei Hook.f. leaves. Here, the crude methanol extract of the leaves along with its organic and aqueous soluble fractions were subjected to different in vitro and in vivo assays. The ethyl acetate soluble fraction exhibited the highest total phenolic content (71.59 mg of GAE/gm of extract) and DPPH free radical scavenging (IC50 value 19.25 μg/ml) whereas another polar fraction; the aqueous soluble extractive showed the maximum cytotoxicity (LC50 value of 18.60 μg/ml) in the brine shrimp lethality assay. In membrane stabilizing activity evaluation, the inhibition of hypotonic solution and heat induced hemolysis were revealed maximum by the DCM (30.64%) and aqueous soluble fraction (39.15%), respectively while the crude methanol extract exhibited the highest thrombolytic potential (30.47% clot lysis). The central analgesic activity of the crude extract significantly increased the latent response time in tail-immersion method (p<0.001) at doses 200 and 400 mg/kg of body weight after 90 minutes of administration when compared to the control group. In acetic acid-induced writhing method, the plant extract showed prominent peripheral analgesia (p<0.001) with 47.54% and 65.57% inhibition at 200- and 400-mg/kg of body weight, respectively. In addition, a dose dependent hypoglycemic and antidiarrheal activities were also observed by the crude extract. Bangladesh Pharmaceutical Journal 24(2): 149-158, 2021

scholarly journals Pharmacological Activities of Senna obtusifolia Linn.: A Medicinal Plant of Bangladesh

2015 ◽  
Vol 17 (2) ◽  
pp. 182-186 ◽  
Author(s):  
Md Anisuzzaman ◽  
Md Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Mohammad Abdur Rashid
Keyword(s):  
Plant Extract ◽  
Pharmaceutical Journal ◽  
Anxiolytic Activity ◽  
Hypotonic Solution ◽  
Pharmacological Activities ◽  
Senna Obtusifolia ◽  
Inhibitory Potential ◽  
Dose Dependent ◽  
Crude Methanol Extract

The crude methanol extract of seed of Senna obtusifolia Linn. has been investigated for anxiolytic, antiatherothrombosis, membrane stabilizing and alpha-amylase inhibitory activities. The anxiolytic activity was examined in mice by using the hole cross and open field test (OFT). The anti-atherothrombosis activity was evaluated and compared with that of standard streptokinase. The membrane stabilizing activity was tested by using hypotonic solution- and heat-induced hemolysis of human erythrocyte. The plant extract was also assessed for anti-diabetic activity through in vitro ?-amylase inhibitory potential. The ?-amylase inhibitory activity of S. obtusifolia was measured using the starch-iodine method. The crude extract of S. obtusifolia showed moderate anxiolytic activity. In the in-vitro anti-atherothrombosis test, the extract exhibited mild activity as compared to the standard, streptokinase (81.53%). In membrane stabilizing activity test, the plant extract at 1.0 mg/ml inhibited the heat-induced hemolysis of RBCs by 56.37% whereas the standard acetyl salicylic acid (ASA) demonstrated 71.36% inhibition of hemolysis. Our results revealed that the extract produced dose-dependent prevention of digestion of carbohydrates by inhibiting ?-amylase. These findings demonstrated that S. obtusifolia may be a good candidate for further analysis because of its effective pharmacological properties. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22338 Bangladesh Pharmaceutical Journal 17(2): 182-186, 2014

scholarly journals Membrane Stabilizing and Preliminary Hypoglycemic Activities of Picrasma javanica

2013 ◽  
Vol 16 (1) ◽  
pp. 89-92
Author(s):  
Tasnuva Sharmin ◽  
Farhana Islam ◽  
Md. Al Amin Sikder ◽  
Shaila Kabir ◽  
Mohammad Rashedul Haque ◽  
...  
Keyword(s):  
Body Weight ◽  
Salicylic Acid ◽  
Carbon Tetrachloride ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

The methanol extract of leaves of Picrasma javanica Blume and its n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were evaluated for membrane stabilizing and preliminary hypoglycemic activities. In hypotonic solution and heat-induced conditions, the crude methanol extract inhibited 65.30±0.52% and 36.79±0.38% haemolysis of RBC as compared to 72.79% and 42.12% produced by standard drug, acetyl salicylic acid, respectively (0.10 mg/ml). The crude extract also showed blood sugar lowering efficacy at 200 and 400 mg/ kg body weight, with 48.63% reduction of blood sugar level at 400 mg/kg body weight while the standard drug, Glibenclamide (10 mg/kg body weight) demonstrated 53.7% reduction of blood sugar level at 3rd hour of administration. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14500 Bangladesh Pharmaceutical Journal 16(1): 89-92, 2013

Antioxidant and Anti-Melanogenic Activities of Pakoba Leaves (Syzygium sp) from North Celebes

2019 ◽  
Vol 2 (2) ◽  
pp. 79-83
Author(s):  
Dela Rosa ◽  
Catherine Roeroe ◽  
Agustina Susanti
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Chloroform Fraction ◽  
Aqueous Fraction ◽  
Ic50 Value ◽  
Dpph Method ◽  
Good Potential ◽  
Crude Methanol Extract

In this research the potential of Pakoba leaf extract to be used as antioxidant and skin whitening (anti-melanogenic agent) is investigated. The antioxidant activity of Pakoba leaves were studied using DPPH method and the result showed that the 80 percent methanol crude extract has strong antioxidant activity with an IC50 value of 22.66 ± 1.02 μg/ml. The aqueous fraction of the sample has an IC50 value of 53.30 ± 1.42 μg/ml, followed by n-butanol fraction (53.63 ± 1.45 μg/ml) and chloroform fraction (511.54 ± 1.59 μg/ml). The anti-melanogenic activity of the crude methanol extract showed IC50 value of 316.56 ± 11.04 μg/ml. Thus, it isconcluded that crude extract of Pakoba leaves shows good potential as the antioxidant source although it does not show good anti- melanogenic activity.

scholarly journals Phytochemical Investigation and In Vitro Antioxidant Activities of Tetracarpidium conophorum (African Walnut) Seeds

2018 ◽  
Vol 6 (2) ◽  
pp. 56-61
Author(s):  
Kelly Oriakhi ◽  
Kissinger Orumwensodia ◽  
Patrick Uadia
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Crude Methanol Extract

Background: Tetracarpidium conophorum (African walnut) is an African plant with ethnobotanical uses. Objectives: The purpose of this study was to evaluate the phytochemical screening and in vitro antioxidant activities of methanol extract and fractions (F) [n-hexane (HEX-F), dichloromethane (DCM-F) and, ethyl acetate (EA-F)] of T. conophorum seeds. Methods: Phytochemical screening and in vitro antioxidant activity study were carried out using DPPH, ABTs radical scavenging assays, nitric oxide inhibitory and reducing potential assays. Results: Methanol extract and its fractions contain phenols, flavonoids, saponins, tannins, terpenoids, and alkaloids. The concentrations of total phenols and flavonoids content were significantly higher in EA-F and crude methanol extract compared to other fractions. Crude methanol and EA-F contain higher concentrations of tannin while hexane fraction had the lowest tannin content but relatively higher proanthocyanidin content compared to other fractions. The antioxidant activity study showed that both methanol crude extract and fractions of T. conophorum seeds have significant activities for DPPH radical scavenging, reducing power, ferric reducing antioxidant potential, nitric oxide inhibitory activities, ABTS and hydroxyl radical scavenging for. DPPH radical scavenging activities of EA-F showed the lowest IC50 of 33.11 µg/mL, followed by Hex-F, DCM-F and crude methanol extract with IC50 of 33.43, 42.09 and 45.44 µg/mL, respectively, when compared to ascorbic acid with IC50 of 17.08 µg/mL. Conclusion: The study showed that T. conophorum seed is a rich source of secondary metabolites, which may be responsible for its antioxidant activities.

scholarly journals Chemical and Biological Investigations of Justicia gendarussa (Burm. f)

2012 ◽  
Vol 10 (1) ◽  
pp. 53-57
Author(s):  
Md Rakib Uddin ◽  
Suchana Sinha ◽  
Md Aslam Hossain ◽  
Md Abul Kaisar ◽  
Md Khalid Hossain ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Whole Plant ◽  
Ic50 Value ◽  
Crude Methanol Extract

In this study, three compounds, stigmasterol (1), lupeol (2), 16-hydroxylupeol (3) were isolated from the petroleum ether soluble fraction of a methanol extract of whole plant of Justica gendarussa (Burm. f). The crude methanol extract as well as its petroleum ether, carbon tetrachloride and chloroform soluble Kupchan fractions were studied for antioxidant, antimicrobial and cytotoxic activities. Among the different fractions tested for antioxidant activity, the chloroform soluble partitionate was the most potent with IC50 value of 18.80 μg/ml as compared to tertbutyl- 1-hydroxytoluene (IC50=17.69 μg/ml). Antimicrobial screening of the different extractives was conducted by the disc diffusion method and all the fractions except chloroform soluble partitionate exhibited mild antimicrobial activity with zone of inhibition ranging from 7-10 mm. In brine shrimp lethality bioassay, the petroleum ether soluble materials demonstrated the highest toxicity with LC50 of 1.27μg /ml. This is the first report of isolation of compounds from J. gendarussa, and its antioxidant, antimicrobial and cytotoxic properties. DOI: http://dx.doi.org/10.3329/dujps.v10i1.10016 DUJPS 2011; 10(1): 53-57

scholarly journals Membrane Stabilizing and Thrombolytic Activities of Sida rhombifolia L.

2015 ◽  
Vol 17 (1) ◽  
pp. 43-45
Author(s):  
Mohammd Firoz Khan ◽  
Sikder Nahidul Islam Rabbi ◽  
Md Aslam Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Salicylic Acid ◽  
Human Erythrocyte ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Thrombolytic Activity ◽  
Whole Plant ◽  
Crude Methanol Extract ◽  
Sida Rhombifolia

The crude methanol extract of whole plant of Sida rhombifolia and different fractions generated from it were tested for membrane stabilizing and thrombolytic activities. In hypotonic solution- and heat-induced conditions, the chloroform soluble partitionates inhibited haemolysis of human erythrocyte by 22.11±0.14% and 64.71±0.08%, respectively as compared to 71.97±0.51% and 40.12±0.29% demonstrated by the standard acetyl salicylic acid (ASA). Moreover, the pet ether soluble materials revealed highest thrombolytic activity with clot lysis value of 19.51±0.03% as compared to 66.98±0.11% exhibited by the standard streptokinase. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22313 Bangladesh Pharmaceutical Journal 17(1): 43-45, 2014

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