Terpenoids from Atylosia scarabaeoides and their antimicrobial activity

1970 ◽  
Vol 4 (2) ◽  
Author(s):  
M Mukhlesur Rahman ◽  
Proma Khondkar ◽  
Alexandar I Gray
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Petroleum Ether ◽  
Key Words ◽  
Full Text ◽  
Inhibitory Concentration ◽  
Petroleum Ether Extract ◽  
2D Nmr ◽  
Ether Extract ◽  
Atylosia Scarabaeoides

Three terpenes-caryophyllene-4,5-oxide, a-amyrin and b-amyrin were isolated from the petroleum ether extract of Atylosia scarabaeoides. The structures of the compounds were confirmed by a series of 1D and 2D NMR and MS analyses. The minimum inhibitory concentration (MIC) of caryophyllene-4,5-oxide was found to be 50-200 mg/ml. Key words: Atylosia scarabaeoides; Papilionaceae; Caryophyllene-4,5-oxide; Chemotaxonomy; Antimicrobial Dhaka Univ. J. Pharm. Sci. Vol.4(2) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIMICROBIAL SCREENING OF SANTALUM ALBUM SEEDS EXTRACTS

2017 ◽  
Vol 9 (10) ◽  
pp. 117
Author(s):  
Gautam P. Vadnere ◽  
Md. Rageeb Usman ◽  
Santram Lodhi ◽  
Vaishali Patil
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Santalum Album ◽  
Ether Extract ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Screening ◽  
Ethanol Extracts

Objective: Present study aimed phytochemical evaluation and antimicrobial screening of petroleum ether and ethanol extracts of Santalum album seeds.Methods: Petroleum ether and ethanol extracts were screened for presence of chemical constituents. Petroleum ether extract was investigated detail by using chromatographic and spectroscopic methods. In vitro antimicrobial activity of both extracts were investigated using disc diffusion method on two gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, gram negative Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans.Results: Santalbic acid was identified in petroleum ether extract and content determined by HPTLC was 4.7%w/w. It was seen that petroleum ether extract have MIC value for B. subtilis, P. aeruginosa, E. coli and C. albicans were 78.125 µg/ml, 19.331 µg/ml, 625 µg/ml & 39.062 µg/ml respectively while MBC was 39.062 µg/ml, 4.882 µg/ml, 312.5 µg/ml & 9.765 µg/ml,  respectively. Petroleum ether extract showed MIC and MBC values for S. aureus was similar as 156.25µg/ml. So, the petroleum ether extract showed significant antimicrobial activity against both gram positive, gram negative and fungal strain.Conclusions: The results of present investigations were indicative of possible high potency of petroleum ether extract due to santalbic acid which could serve as chemotherapeutic agent.

scholarly journals PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF THE LEAVES OF LANNEA KERSTINGII ENGL & K. KRAUSE (ANACADIACEAE)

2014 ◽  
Vol 04 (04) ◽  
pp. 004-009
Author(s):  
Njinga NS ◽  
Sule MI ◽  
Pateh UU ◽  
Hassan HS ◽  
Ahmad MM ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Chloroform Fraction ◽  
Ether Extract ◽  
Minimum Fungicidal Concentration

AbstractThe phytochemical and antimicrobial activity of the petroleum ether and crude methanol extracts, chloroform and ethyl acetate fractions of the leaves of Lannea kerstingii were investigated. Phytochemical screening revealed the presence of steroids and triterpenes in the petroleum ether extract, steroid, triterpene, flavonoids and tannins in both crude methanol extract and chloroform fraction while the ethyl acetate fraction contained only flavonoids and tannins. The extracts exhibited antimicrobial activities with zones of inhibition ranging from 17.00 to 21.03, 20.10 to 25.24, 25.32 to 34.02 and 22.28 to 27.20 mm for petroleum ether extract, methanol extract, chloroform and ethyl acetate fractions respectively. The minimum inhibitory concentration was between 5 and 10mg/ml, 5mg/ml for the petroleum ether and methanol extract respectively, and between 2.5 and 5 mg/ml, 5mg/ml for the acetate fractions. The minimum bactericidal concentration for all the extracts was 40mg/ml respectively except for chloroform fraction which ranged from 20 to 40mg/ml. The minimum fungicidal concentration for all the extracts was found to be 40mg/ml respectively. This result indicates the broad spectrum antimicrobial potential of L. Kerstingii and justifies the use of this plant in traditional medicine.

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY AGAINST CLINICAL PATHOGEN OF MEDICINALLY IMPORTANT ORCHID CALANTHE MASUCA (D.DON) LINDL

2021 ◽  
pp. 5-8
Author(s):  
V. Nandagopalan ◽  
D. Kavitha
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Ether Extract ◽  
Wide Range

Calanthe masuca (D.Done) Lindl is a potential medicinal plant of terrestrial orchids. Tribal peoples were used the plants for therapeutic purpose. The present study was to evaluate the phytochemical analysis and Antimicrobial Activity of Calanthe masuca in leaf extracts. Preliminary phytochemical analysis revealed that the presence of bioactive substance in leaf extract was found that Alkaloids, Flavanoids, Phenol, Tannins, Saponins, Steroids, Terpenoids, Carboxcylic acid, Glycoside and Mucilage. Antimicrobial activity by Agar well diffusion method again six pathogenic microbes such as Staphylococcus aureus, Bacillus subtilis, E.coli and Pseudomonas aeruginosa in bacteria, Aspergillus niger and Candida albicans in fungi by using different solvent such as Aqueous, Methanol and Petroleum ether. It was observered that Methanol extract showed highest activity against the pathogen such as S.aureus (13.5 mm), B.subtilis (12.4 mm), E.coli (12.5 mm) and P.aeruginosa (13.0 mm) and then followed by the Aqueous extract and low activity showed in petroleum ether extract. The methanol extract showed signicant Antifungal activity against Aspergillus niger (14.0mm) and Candida albicans (13.0 mm) with higher concentration and Petroleum ether extract showed lower activity in fungi. The capacity of the crude concentrates of Calanthe masuca to suppress the development of microorganism which means that it has a wide range of antimicrobial potential utilized for various cures.

The Structure Elucidation and Antimicrobial Activities of Nonsterol Triterpenoids from Ixeris chinensis

2014 ◽  
Vol 69 (9-10) ◽  
pp. 1021-1025 ◽  
Author(s):  
Qing-Hu Wang ◽  
Na-Ren-Chao-Ke-Tu Han ◽  
Na-Yin-Tai Dai ◽  
Xiao-Lan Wu ◽  
Wen-Quan Tai ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Structure Elucidation ◽  
Petroleum Ether Extract ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Spectroscopic Methods ◽  
Ether Extract ◽  
Esi Ms ◽  
Nmr Techniques ◽  
First Time

Abstract An investigation of the petroleum ether extract from Ixeris chinensis Nakai has led to the isolation of a new compound, 3-O-acetyl-22,28-cyclobauer-7-en-3β-ol (1), together with four known compounds which have been isolated from this plant for the first time, namely taraxast-20-en-3β-ol (2), 3-O-acetyl-taraxast-20-en-3β-ol (3), taraxast-20(30)-en-3β-ol (4), and 3-O-acetyl-taraxast-20(30)- en-3β-ol (5). The structures of the isolated compounds have been elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D NMR, 2D NMR techniques, and by comparison with data reported in the literature. All compounds have been evaluated for their activity against various bacteria and shown to give good results

scholarly journals Antiviral and cytotoxic activities of anthraquinones isolated from cassia roxburghii linn. leaves

2013 ◽  
Vol 59 (4) ◽  
pp. 33-44 ◽  
Author(s):  
Magdy M. D. Mohammed ◽  
Sahar S. El-Souda ◽  
Salwa M. El-Hallouty ◽  
Nobuyuki Kobayashi
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Ether Extract ◽  
Separation And Purification ◽  
Bioassay Guided Fractionation ◽  
Hct 116 ◽  
Aloe Emodin ◽  
Mcf 7

Abstract The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. against HCT-116 and MCF-7 cell lines resulted with IC50=34.9 and 38.04 μg/ml, respectively, while against HepG-2 showed no activity. A bioassay guided-fractionation approach was conducted to isolate and identify the active cytotoxic principles. Further chromatographic separation and purification of the petroleum ether extract resulted in the isolation of two anthraquinones identified as aloe-emodin acetate and aloe-emodin, along with stigmasterol, β-sitosterol and palmitic acid. The structure elucidation of isolated compounds was performend using 1D, 2D-NMR and HR-MS. Furthermore, the cytotoxicity of aloe-emodin acetate and aloe-emodin were evaluated and resulted with IC50=153.30 and 70.02 μg/ml against HCT-116 and with 93.20 and 53.20 μg/ml against MCF-7, respectively, while against HepG-2 showed no activity. Moreover, the antiviral activity of the two isolated anthraquinones was tested against influenza virus-A, and resulted with IC50=10.23 as well as 2.00 and with CC50=1.32 and 0.47 μg/ml, respectively.

scholarly journals A New Sesquiterpenoid Aminoquinone from an Indonesian Marine Sponge

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 158 ◽  
Author(s):  
Walter Balansa ◽  
Ute Mettal ◽  
Zerlina Wuisan ◽  
Anuchit Plubrukarn ◽  
Frans Ijong ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Data Analysis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Micrococcus Luteus ◽  
2D Nmr ◽  
Cytotoxic Effects ◽  
Sar Studies ◽  
Structure Activity

Sponges are a well-known bioresource for bioactive compounds. In this study, antibacterial activity-guided fractionation of the extract from an Indonesian marine Dactylospongia elegans sponge led to the discovery of four merosesquiterpenoids, namely, a new sesquiterpenoid aminoquinone nakijiquinone V (1), along with illimaquinone (2), smenospongine (3), and dyctioceratine C (4). The structure of compound 1 was elucidated by 1D and 2D NMR as well as by LC-HRESIMS data analysis. Compounds 2–4 showed moderate to low antimicrobial activity against Bacillus megaterium DSM32 with a minimum inhibitory concentration (MIC) of 32 μg/mL, 32 μg/mL, and 64 μg/mL, respectively. Furthermore, compounds 2 and 3 both inhibited Micrococcus luteus ATCC 4698 with a MIC of 32 μg/mL. In conclusion, the isolated merosesquiterpenoids, which are known for their cytotoxic effects, showed antibacterial activity and prompt future structure activity relationship (SAR) studies concerning the various bioactivities observed for this group of natural products.

scholarly journals Phytochemical and Antimicrobial Activity ofDigera Muricata(L.) Mart.

2010 ◽  
Vol 7 (1) ◽  
pp. 275-280 ◽  
Author(s):  
Pratima Mathad ◽  
Sundar S. Mety
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Distilled Water ◽  
Ether Extract ◽  
Zone Of Inhibition

The successive Soxhlet extract ofDigera muricata(L.) Mart. (Amaranthaceae) were extracted using petroleum ether, chloroform, ethanol and distilled water in ascending order of the polarity. These extracts were subjected to screening of preliminary phytochemicals tests. The results indicate the presence of alkaloids, flavonoids, phenols, tannins, terpenes and saponins. These extracts further subjected to the antimicrobial activity. Among the bacteria used, the petroleum ether extract gave highest zone of inhibition at 400 µg/well againstV. cholerae.Similarly, in fungi the ethanol extract exhibited highest zone of inhibition at 400 µg/well againstCandida albicans.

scholarly journals Evaluation of Anthelmentic and Antimicrobial Activity of the Extract of the Root of the Plant Jasminum multiflorum(Andr.)

2020 ◽  
Vol 10 (4) ◽  
pp. 157-160
Author(s):  
Sandip Kumar Pahari ◽  
B.K. Gupta ◽  
R. Debnath ◽  
A. Das
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Qualitative Evaluation ◽  
Maximum Activity ◽  
Klebsiella Pneumonia ◽  
Ether Extract ◽  
Zone Of Inhibition

As per qualitative evaluation in different solvents of the root of the plant satisfies the presence of cardiac glycosides along with trace quantities of steroid and saponins. Among them the petroleum ether extract of the root of the plant Jasminum multiflorum was evaluated for anthelmintic activity and the ethanolic extract was evaluated for antimicrobial activity.1,2 Traditionally this species are used in indolent ulcer, pitta and inflammation. Only few CNS activity are reported on ethanolic extract of aerial part of the plant, though the root of the plant is more potent as per folkore claim. The petroleum ether extract was investigated for anthelmentic activity using earthworm (Pheretima posthuma) at different concentration (5mg/ml – 50 mg/ml). As standard albendazole suspension (10mg/ml) and 3% solution of normal saline was used as control. The death and paralysis time were recorded and compared. Extract exhibit significant anthelmentic activity at (100mg/ml) concentration and found effective.1,3,4The ethanolic fraction of the root of the plant was collected and evaporated to dryness under vacuum to avoid the presence of even less quantity of ethanol in the extract. Two Gram (+ve) bacteria namely Bacillius subtilis, Staphylococcus aureus and two Gram (-ve) ve bacteria namely Escherichia coli and Klebsiella pneumonia were selected for the estimation of antimicrobial activity depending on zone of inhibition. It was seen that 100mg/ml concentration of the extract showed maximum activity against Klebsiella pneumonia with a zone of inhibition 0.5mm and for others it ranged from 0.3 to 0.4 mm. 1,4,5 Keywords: Jasminum multiforum; anthelmintic; antimicrobial; zone of inhibition

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND TO EVALUATE THE ANTI-MICROBIAL ACTIVITY OF HYDRO-ALCOHOLIC and PETROLEUM ETHER EXTRACT OF JASMINE ROOT (NYCTANTHES ARBOUR-TRISTIS). (FAMILY-NYCTAGINACEAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 47
Author(s):  
Abdul Samim ◽  
Sumit Das
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Microbial Activity ◽  
Plant Extract ◽  
Petroleum Ether Extract ◽  
Phytochemical Analysis ◽  
Disc Diffusion ◽  
Ether Extract ◽  
Bacterial Strains ◽  
Phytochemical Constituents

Objective: To estimate the anti-microbial activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared and studied for phytochemical constituents by using various standard methods. The antimicrobial activity of plant extract was performed on two bacterial strains and one fungal strain using disc diffusion method.Results: The present study shows the phytochemical analysis, antimicrobial activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol, terpenoids, glycosides, saponins respectively. The anti-microbial activity of the plant extract showed significant results against all three of the test organisms.Conclusion: The present study concluded that the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis (night flowering jasmine) contains the highly presence of Phytochemical constituents. The hydro-alcoholic and petroleum ether extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

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