scholarly journals Evaluation of Rice Germplasm under Salt Stress at the Seedling Stage through SSR Markers

2013 ◽  
Vol 3 (1) ◽  
pp. 52-59 ◽  
Author(s):  
M Al-Amin ◽  
MM Islam ◽  
SN Begum ◽  
MS Alam ◽  
M Moniruzzaman ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Salinity Tolerance ◽  
Dry Matter ◽  
Growth Parameters ◽  
Seedling Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Genotypes ◽  
Salt Susceptible

Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding. Int. J. Agril. Res. Innov. & Tech. 3 (1): 52-59, June, 2013 DOI: http://dx.doi.org/10.3329/ijarit.v3i1.16093

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

scholarly journals Screening of Salinity Tolerance of Twenty Rice Genotypes at the Seedling Stage Through Hydroponic and Ssr Marker

2021 ◽  
Vol 8 (1) ◽  
pp. 75-88
Author(s):  
Sharif Ahmad Al Muti ◽  
Md Shahidul Islam ◽  
Md Monirul Islam ◽  
Md Mahmud Al Noor ◽  
SM Abdul Alim ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Dry Matter ◽  
Ssr Marker ◽  
Shoot Length ◽  
Seedling Stage ◽  
Salt Tolerant ◽  
The World ◽  
Tolerant Variety ◽  
Rice Genotypes ◽  
Nei's Genetic Distance

Salinity is one of the major abiotic stresses which severely affect the production of crops across the world. In this experiment, we examined 20 rice genotypes of diverse origins and sources including few salt tolerant varieties (Binadhan-8, Binadhan-10, Pokkali and FL478) as check. The main objective of this study was to determine salt tolerance at seedling stage and to evaluate genetic variation using SSR markers. IRRI standard protocol was applied to screen out salinity among those varieties, at the glasshouse of Bangladesh Institute of Nuclear Agriculture, BAU campus, Mymensingh-2202. Shoot length, root length and total dry matter were recorded at 6dS/m, 8dS/m, 10dS/m and 12 dS/m salt stress levels. According to the morphological and molecular survey of 20 rice genotypes at the seedling stage it was evident that, Binadhan-8, Binadhan-10, Pokkali, FL478, IR64, IR4630, FR13A and Sadamota identified as salt tolerant whereas THDB, Moulata, MV-20, CPD-23, CPD-29, Pot-18, Pot-27 and Dudkalam those were found as susceptible, BRRI dhan67, Binadhan-17 and Binadhan-21 those were traced as highly susceptible. The highest Nei’s genetic distance value 1.0 was found in Moulata vs Sadamota and the lowest value 0.08 was observed in Binadhan-21 vs IR64. It will be used in future breeding program to develop a saline tolerant variety of rice. Res. Agric., Livest. Fish.8(1): 75-88, April 2021

Evaluation of Rice Genotypes at Seedling Stage for Salinity Tolerance

2002 ◽  
Vol 1 (2) ◽  
pp. 117-118 ◽  
Author(s):  
Yousaf Ali . ◽  
A. R. Awan . ◽  
M. Y. Ashraf .
Keyword(s):  
Salinity Tolerance ◽  
Seedling Stage ◽  
Rice Genotypes

scholarly journals Screening of Some Rice (Oryza sativa L.) Genotypes for Salinity Tolerance using Morphological and Molecular Markers

2019 ◽  
Vol 16 (2) ◽  
pp. 377-390
Author(s):  
Shahabuddin Ahmed ◽  
Touhidur Rahman Anik ◽  
Ashraful Islam ◽  
Imtiaz Uddin ◽  
Mohammad S. Haque
Keyword(s):  
Oryza Sativa L ◽  
Rice Variety ◽  
Dry Weight ◽  
Crop Productivity ◽  
Seedling Stage ◽  
Group Method ◽  
Salt Tolerant ◽  
Pair Group ◽  
Salinity Levels ◽  
Rice Genotypes

Salinity is one of the major abiotic stresses, which adversely affects the crop productivity. Thirty rice genotypes of diverse origin including three salt tolerant check varieties, Binadhan-8, Binadhan-10 and Pokkali, were used to evaluate salt tolerance at seedling stage and to determine the genetic diversity using microsatellite markers. Salinity screening was done at the seedling stage using hydroponic system following IRRI standard protocol. Three salinity levels as 6dSm-1, 8dSm-1, and 10dSm-1 were used along with control. Data were recorded on root length, shoot length and dry weight and the genotypes were scored based on modified standard evaluation score (SES) for visual injury. Sixteen SSR markers were used to study the genetic variation within 30 rice genotypes. A total of 65 alleles with an average of 4.06 allele per locus were detected among 30 rice genotypes. The polymorphism information content (PIC) value ranged from 0.24 to 0.86 with an average of 0.51. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed four clusters. Among them cluster I identified 5 salt tolerant genotypes and cluster IV separated one tolerant and one moderately tolerant genotype. Based on SES evaluation and molecular analysis genotypes Balam, THDB, Q-31, Ab.Hai, BR-5, FR13A ware salt tolerant; Moulota, Super hybrid, Y-1281, Binadhan-16 were moderate salt tolerant. This information could be useful for selection of suitable genotypes for developing salt tolerant rice variety through molecular breeding.

scholarly journals Phenotypic and genotypic screening of rice genotypes at reproductive stage for salt tolerance

2018 ◽  
Vol 15 (2) ◽  
pp. 69-80
Author(s):  
B Hossen ◽  
MS Haque ◽  
K Miah ◽  
MZ Tareq
Keyword(s):  
Salt Tolerance ◽  
Gene Pyramiding ◽  
Reproductive Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Tolerance Level ◽  
Ssr Primers ◽  
Genetic Clusters ◽  
Rice Genotypes ◽  
Cluster 2

Salinity screening of 24 rice genotypes was performed at the reproductive stage for evaluating their salt tolerance level. On the basis of yield and yield components, genotypes were categorized as tolerant, susceptible and moderately tolerant. PBRC-30, Ashfal, Horkuch, STL-20 and Pokkali were found as tolerant while Binadhan-7, S-39 L-11, S-37 L- 27, S-37 L-36 and S-37 L-39 were found as susceptible. Selected three SSR markers viz. RM336, RM21 and RM510 were used to determine salinity tolerance. The genetic diversity was ranges from 0.8194 to 0.8854 with an average of 0.8530. The highest PCI value was 0.8742 and the lowest was 0.8004 from RM510 and RM21, respectively. The UPGMA clustering system generated six genetic clusters. The highest genetically dissimilarity of (Cluster 1) vs (Cluster 2 sub-cluster A) and the crossing would be helpful for salt tolerant rice development. Thus, selected SSR primers and genotypes would be useful in marker assisted breeding, quantitative trait loci (QTL) mapping and gene pyramiding in breeding programmed for improvement of rice for salt tolerance.SAARC J. Agri., 15(2): 69-80 (2017)

scholarly journals Genomic and transcriptomic analysis reveal molecular basis of salinity tolerance in a novel strong salt-tolerant rice landrace Changmaogu

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Bing-Rui Sun ◽  
Chong-Yun Fu ◽  
Zhi-Lan Fan ◽  
Yu Chen ◽  
Wen-Feng Chen ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Molecular Basis ◽  
Carotenoid Biosynthesis ◽  
Seedling Stage ◽  
Differentially Expressed ◽  
Salt Tolerant ◽  
Transcriptomic Sequencing ◽  
Rice Landrace

Abstract Background Salt stress is an important factor that limits rice yield. We identified a novel, strongly salt tolerant rice landrace called Changmaogu (CMG) collected from a coastal beach of Zhanjiang, Guangdong Province, China. The salt tolerance of CMG was much better than that of the international recognized salt tolerant rice cultivar Pokkali in the germination and seedling stages. Results To understand the molecular basis of salt tolerance in CMG, we performed BSA-seq for two extreme bulks derived from the cross between CMG and a cultivar sensitive to salt, Zhefu802. Transcriptomic sequencing was conducted for CMG at the germination and young seedling stages. Six candidate regions for salt tolerance were mapped on Chromosome 1 by BSA-seq using the extreme populations. Based on the polymorphisms identified between both parents, we detected 32 genes containing nonsynonymous coding single nucleotide polymorphisms (SNPs) and frameshift mutations in the open reading frame (ORF) regions. With transcriptomic sequencing, we detected a large number of differentially expressed genes (DEGs) at the germination and seedling stages under salt stress. KEGG analysis indicated two of 69 DEGs shared at the germination and seedling stages were significantly enriched in the pathway of carotenoid biosynthesis. Of the 169 overlapping DEGs among three sample points at the seedling stage, 13 and six DEGs were clustered into the pathways of ABA signal transduction and carotenoid biosynthesis, respectively. Of the 32 genes carrying sequence variation, only OsPP2C8 (Os01g0656200) was differentially expressed in the young seedling stage under salt stress and also showed sequence polymorphism in the ORFs between CMG and Zhefu802. Conclusion OsPP2C8 was identified as the target candidate gene for salinity tolerance in the seedling stage. This provides an important genetic resource for the breeding of novel salt tolerant rice cultivars.

scholarly journals SALINITY TOLERANCE OF SEVERAL RICE GENOTYPES AT SEEDLING STAGE

2018 ◽  
Vol 18 (2) ◽  
pp. 63
Author(s):  
Heni Safitri ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Sintho Wahyuning Ardie
Keyword(s):  
Salinity Tolerance ◽  
Block Design ◽  
Seedling Stage ◽  
Salinity Level ◽  
Growth And Yield ◽  
Future Research ◽  
Nutrient Culture ◽  
Two Factors ◽  
Check Variety ◽  
Rice Genotypes

<p class="abstrakinggris">Salinity is one of the most serious problems in rice cultivation. Salinity drastically reduced plant growth and yield, especially at seedling stage. Several rice genotypes have been produced, but their tolerance to salinity has not yet been evaluated. The study aimed to evaluate salinity tolerance of rice genotypes at seedling stage. The glasshouse experiment was conducted at Cimanggu Experimental Station, Bogor, from April to May 2013. Thirteen rice genotypes and two check varieties, namely Pokkali (salt tolerant) and IR29 (salt sensitive) were tested at seedling stage. The experiment was arranged in a randomized complete block design with three replications and two factors, namely the levels of NaCl (0 and 120 mM) and 13 genotypes of rice. Rice seedlings were grown in the nutrient culture (hydroponic) supplemented with NaCl at different levels. The growth and salinity injury levels of the genotypes were recorded periodically. The results showed that salinity level of 120 mM NaCl reduced seedling growth of all rice genotypes, but the tolerant ones were survived after 14 days or until the sensitive check variety died. Based on the visual injury symptoms on the leaves, five genotypes, i.e. Dendang, Inpara 5, Inpari 29, IR77674-3B-8-2-2-14-4-AJY2, and IR81493-BBB-6-B- 2-1-2 were tolerant to 120 mM salinity level, while Inpara 4 was comparable to salt sensitive IR29. Hence, Inpara 4 could be used as a salinity sensitive genotype for future research of testing tolerant variety. Further evaluation is needed to confirm their salinity tolerance under field conditions. </p>

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