scholarly journals Phenotypic and genotypic screening of rice genotypes at reproductive stage for salt tolerance

2018 ◽  
Vol 15 (2) ◽  
pp. 69-80
Author(s):  
B Hossen ◽  
MS Haque ◽  
K Miah ◽  
MZ Tareq
Keyword(s):  
Salt Tolerance ◽  
Gene Pyramiding ◽  
Reproductive Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Tolerance Level ◽  
Ssr Primers ◽  
Genetic Clusters ◽  
Rice Genotypes ◽  
Cluster 2

Salinity screening of 24 rice genotypes was performed at the reproductive stage for evaluating their salt tolerance level. On the basis of yield and yield components, genotypes were categorized as tolerant, susceptible and moderately tolerant. PBRC-30, Ashfal, Horkuch, STL-20 and Pokkali were found as tolerant while Binadhan-7, S-39 L-11, S-37 L- 27, S-37 L-36 and S-37 L-39 were found as susceptible. Selected three SSR markers viz. RM336, RM21 and RM510 were used to determine salinity tolerance. The genetic diversity was ranges from 0.8194 to 0.8854 with an average of 0.8530. The highest PCI value was 0.8742 and the lowest was 0.8004 from RM510 and RM21, respectively. The UPGMA clustering system generated six genetic clusters. The highest genetically dissimilarity of (Cluster 1) vs (Cluster 2 sub-cluster A) and the crossing would be helpful for salt tolerant rice development. Thus, selected SSR primers and genotypes would be useful in marker assisted breeding, quantitative trait loci (QTL) mapping and gene pyramiding in breeding programmed for improvement of rice for salt tolerance.SAARC J. Agri., 15(2): 69-80 (2017)

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

scholarly journals Evaluation of salinity tolerance indices in North African barley accessions at reproductive stage

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Dorsaf Allel ◽  
Anis BenAmar ◽  
Mounawer Badri ◽  
Chedly Abdelly
Keyword(s):  
Salt Tolerance ◽  
Grain Yield ◽  
Salinity Tolerance ◽  
Selection Criteria ◽  
Reproductive Stage ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
North African ◽  
Grain Number ◽  
Selection Indices

Soil salinity is one of the main factors limiting cereal productivity in worldwide agriculture. Exploitation of natural variation in local barley germplasm is an effective approach to overcome yield losses. Three gene pools of North African Hordeum vulgare L. grown in Tunisia, Algeria and Egypt were evaluated at the reproductive stage under control and saline conditions. Assessment of stress tolerance was monitored using morphological, yield-related traits and phenological parameters of reproductive organs showing significant genetic variation. High heritability and positive relationships were found suggesting that some traits associated with salt tolerance could be used as selection criteria. The phenotypic correlations revealed that vegetative traits including shoot biomass, tiller number and leaf number along with yield-related traits such as spike number, one spike dry weight, grain number/plant and grain number/spike were highly positively correlated with grain yield under saline conditions. Hence, these traits can be used as reliable selection criteria to improve barley grain yield. Keeping a higher shoot biomass and longer heading and maturity periods as well as privileged filling ability might contribute to higher grain production in barley and thus could be potential target traits in barley crop breeding toward improvement of salinity tolerance. Multiple selection indices revealed that salt tolerance trait index provided a better discrimination of barley landraces allowing selection of highly salt-tolerant and highly productive genotypes under severe salinity level. Effective evaluation of salt tolerance requires an integration of selection indices to successfully identify and characterize salt tolerant lines required for valuable exploitation in the management of salt-affected areas.  

scholarly journals Evaluation of Rice Germplasm under Salt Stress at the Seedling Stage through SSR Markers

2013 ◽  
Vol 3 (1) ◽  
pp. 52-59 ◽  
Author(s):  
M Al-Amin ◽  
MM Islam ◽  
SN Begum ◽  
MS Alam ◽  
M Moniruzzaman ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Salinity Tolerance ◽  
Dry Matter ◽  
Growth Parameters ◽  
Seedling Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Genotypes ◽  
Salt Susceptible

Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding. Int. J. Agril. Res. Innov. & Tech. 3 (1): 52-59, June, 2013 DOI: http://dx.doi.org/10.3329/ijarit.v3i1.16093

scholarly journals Whole genome sequence analysis of rice genotypes with contrasting response to salinity stress

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Prasanta K. Subudhi ◽  
Rama Shankar ◽  
Mukesh Jain
Keyword(s):  
Salt Tolerance ◽  
Dna Polymorphisms ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Rice Varieties ◽  
Genome Wide ◽  
Rice Genotypes ◽  
Genome Level

AbstractSalinity is a major abiotic constraint for rice farming. Abundant natural variability exists in rice germplasm for salt tolerance traits. Since few studies focused on the genome level variation in rice genotypes with contrasting response to salt stress, genomic resequencing in diverse genetic materials is needed to elucidate the molecular basis of salt tolerance mechanisms. The whole genome sequences of two salt tolerant (Pokkali and Nona Bokra) and three salt sensitive (Bengal, Cocodrie, and IR64) rice genotypes were analyzed. A total of 413 million reads were generated with a mean genome coverage of 93% and mean sequencing depth of 18X. Analysis of the DNA polymorphisms revealed that 2347 nonsynonymous SNPs and 51 frameshift mutations could differentiate the salt tolerant from the salt sensitive genotypes. The integration of genome-wide polymorphism information with the QTL mapping and expression profiling data led to identification of 396 differentially expressed genes with large effect variants in the coding regions. These genes were involved in multiple salt tolerance mechanisms, such as ion transport, oxidative stress tolerance, signal transduction, and transcriptional regulation. The genome-wide DNA polymorphisms and the promising candidate genes identified in this study represent a valuable resource for molecular breeding of salt tolerant rice varieties.

Fundamental parenchyma cells are involved in Na+ and Cl– removal ability in rice leaf sheath

2019 ◽  
Vol 46 (8) ◽  
pp. 743 ◽  
Author(s):  
Sarin Neang ◽  
Marjorie de Ocampo ◽  
James A. Egdane ◽  
John D. Platten ◽  
Abdelbagi M. Ismail ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Tissue Level ◽  
Leaf Sheath ◽  
Salt Sensitivity ◽  
Salt Tolerant ◽  
Basal Leaf ◽  
Rice Plants ◽  
Rice Leaf ◽  
Parenchyma Cells ◽  
Rice Genotypes

Salt sensitivity in rice plants is associated with the accumulated amount of Na+ and Cl– in shoots and, more significantly, in photosynthetic tissues. Therefore, salt removal ability at the leaf sheath level is an important mechanism of salt tolerance. In the present study we attempted to determine whether rice leaf sheaths excluded Cl– as well as Na+, and to identify the tissues that were involved in the removal ability of both ions. In two rice genotypes, salt-tolerant FL478 and -sensitive IR29, leaf sheaths excluded Na+ and Cl– under NaCl treatment as estimated using their sheath:blade ratios. The sheath:blade ratio of Na+ but not of Cl–, was increased by NaCl treatment. Under NaCl treatment, Na+ concentration was higher in the basal leaf sheath, whereas Cl– concentration was higher in the middle and tip parts. At the tissue level, fundamental parenchyma cells of leaf sheaths retained the highest amounts of Na and Cl when treated with high amount of NaCl. These results imply that the leaf sheath potentially functions to remove excess Na+ and Cl– from xylem vessels in different locations along the axis, with the fundamental parenchyma cells of leaf sheaths being involved in over-accumulation of both Na+ and Cl–.

scholarly journals Screening of Rice (Oryza sativa L.) Genotypes at Reproductive Stage for their Tolerance to Salinity

2018 ◽  
Vol 16 (1) ◽  
pp. 65-77
Author(s):  
M M Islam ◽  
M H Faruqe ◽  
M S Rana ◽  
M Akter ◽  
M A Karim
Keyword(s):  
Oryza Sativa ◽  
Salt Tolerance ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Saline Water ◽  
Coastal Zones ◽  
Salt Tolerant ◽  
Randomized Design ◽  
Rice Genotypes ◽  
Four Levels

Soil salinity is one of the most devastating environmental stresses for rice production in the coastal areas of Bangladesh. Improvement in salt tolerance of rice is an important way for the economic utilization of coastal zones. An experiment was conducted at the vinyl house of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during December 2016 to April 2017 to determine the effects of different salinity levels on the yield and yield components of some rice (Oryza sativa L.) genotype sand finally, screening of rice genotypes for salt tolerance. The experiment containing four treatments was laid out in a complete randomized design with five replications. The treatments were four levels of saline water with electrical conductivities at control (0.3 dSm-1), 5, 10 and 15dSm-1. Based on screening at germination stage, relatively salt-tolerant eleven genotypes with two check varieties were used in this experiment. The study showed that increase in salinity level significantly decreased yield and yield contributing characters of rice. However, among the eleven, three genotypes (Chapali, Patnai 23 and Soloi) were considered as moderately salt tolerant at 15 dSm-1 on the basis of their yields and yield contributing characteristics such as plant height reduction, total tiller reduction, effective tiller reduction, reduction of fertile grains per panicle, grain yield and relative grain yield. Therefore, Chapali, Patnai 23 and Soloi might be recommended as moderately salt tolerant rice genotypes. The Agriculturists 2018; 16(1) 65-77

scholarly journals Genetic Analysis of F4 Rice Lines for Salt Tolerance at the Reproductive Stage

2013 ◽  
Vol 21 (1-2) ◽  
pp. 31-38 ◽  
Author(s):  
S Akhtar ◽  
MM Islam ◽  
SN Begum ◽  
J Halder ◽  
MK Alam ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Grain Yield ◽  
Reproductive Stage ◽  
Genetic Advance ◽  
Salt Tolerant ◽  
Additive Gene ◽  
High Heritability ◽  
Selection For ◽  
Salt Susceptible ◽  
Selection Of

A total of 29 lines of F4 population of rice along with their parents Binadhan-5 (high yielding and salt susceptible) and Harkuch (salt tolerant landrace) were evaluated for salt tolerance at the reproductive stage with EC 6 dS/m following IRRI standard protocol. High heritability coupled with high genetic advance was observed in plant height in salinized and non-salinized conditions. High heritability along with high genetic advance was also found in number of filled grains/plant in saline condition. These characters were under additive gene control and selection for salt tolerance might be effective. Number of filled grains/plant showed significant positive correlation with grain yield/plant. Path analysis revealed that number of filled grains/plant had positive and maximum direct effect on grain yield/plant. Therefore, number of filled grains/plant should be given the importance in selection of rice lines under saline condition.DOI: http://dx.doi.org/10.3329/pa.v21i1-2.16746 Progress. Agric. 21(1 & 2): 31 - 38, 2010

scholarly journals Evaluation of Rice Genotypes for Salinity Tolerance at Reproductive Stage Using Phenotypic and Molecular Markers

2020 ◽  
pp. 54-62
Author(s):  
Anuj Kumar ◽  
D. K. Dwivedi ◽  
Pradeep K. Bharti ◽  
Vineeta Singh ◽  
Preeti Kumari ◽  
...  
Keyword(s):  
Crop Production ◽  
Reproductive Stage ◽  
Saline Condition ◽  
Panicle Length ◽  
Salt Tolerant ◽  
Environmental Constraint ◽  
Breeding Programmes ◽  
Biological Yield ◽  
Rice Genotypes ◽  
Variance Estimates

Rice is the single largest source of food energy for more than half of the world’s population. Salinity may be a serious environmental constraint to crop production. Salinity screening of twenty rice genotypes were performed at the reproductive stages, in the net house of department of PMB&GE. Phenotyping of the genotypes was done at EC 12dS/m at reproductive stage in net house. Most desirable genotypes days to 50% flowering were IR-68144-2B-2-2-3-1-120, CSR-13, FL-478, NDR-359, AYYAR and NUD-2, SAMBHA MANSURI and MTU-1010; for plant height IR-68144-2B-2-2-3-1-127, NUD-3, NUD-2, NDRK-2008, IR-91171-66-3-2-1-3, SAMBHA MANSURI, TARAMON and  MTU-1010; for panicle bearing tillers/plant FL-478, NDR-359 and SWARNA; for panicle length IR-68144-2B-2-2-3-1-127, IR-91167-99-1-1-1-3, IR-29, FL-478, NDRK-2008 and IR-92953-49-1-3; for spikelets/panicle IR-91167-99-1-1-1-3, NDRK-2008, SWARNA , IR-92953-49-1-3, IR-91171-66-3-2-1-3, IR-83668-35-2-2-2 and MTU-1010; for grains/panicle SWARNA, IR-92953-49-1-3, IR-91171-66-3-2-1-3, IR-83668-35-2-2-2, NDRK-2008 and MTU-1010 for spikelet fertility % NUD-3, IR-29, FL-478, NDRK-2008, SWARNA, IR-91171-66-3-2-1-3 and IR-83668-35-2-2-2; for test weight  NUD-3, NDRK-2008, IR-29 and SWARNA for biological yield/plant AYYAR, TARAMON and NUD-3; for harvest index FL-478, IR-68144-2B-2-2-3-1-120, IR-91167-133-1-1-2-3 and NUD-2; for Na+ NUD-3 and FL-478; for K+ IR-91167-133-1-1-2-3, NDR-359 and MTU-1010; for Na+/K+ IR-68144-2B-2-2-3-1-127, IR-91167-99-1-1-1-3, NUD-3, FL-478, IR-64 and SAMBHA MANSURI; for grain yield/ plant (g) AYYAR and FL-478  were reported highly significant in mean performance for yield and its components traits. Analysis of variance, estimates of phenotypic and genotypic coefficient of variation, estimates of heritability and genetic advance in percent of mean were recorded for all the characters among 20 rice genotypes in saline condition. Molecular analysis with SSR markers differentiates the rice genotypes into tolerant and susceptible based on banding pattern. The tolerant rice genotypes were NUD 3, IR-68144-2B-2-2-3-166, IR68144-2B-2-2-3-1-120, IR68100-2B-2-2-3-1-127, IR-1167-31-3-1-33 and IR-91171-66-3-2-1-3 and susceptible were NDR-359, Taramon, MTU-1010,  Swarna and IR-64. The identified salt tolerant genotypes can be potential germplasm sources for future breeding programmes.

scholarly journals Variable Effects of Silicon on Salt Tolerant Indices in Rice Genotypes at Seedling Stage

2020 ◽  
pp. 43-55
Author(s):  
Rinny Swain ◽  
Surabhika Panda ◽  
Gyana Ranjan Rout
Keyword(s):  
Salt Tolerance ◽  
Salinity Stress ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Seedling Stage ◽  
Antioxidant Enzyme Activities ◽  
Salt Tolerant ◽  
Silicon Accumulation ◽  
Rice Genotypes ◽  
Tolerance Indices

Silicon (Si) is known to improve salt tolerance in rice. However, the correlation of silicon with different physiological and biochemical indices of salt tolerance is not properly understood. Two rice genotypes with different silicon accumulation ability were evaluated along with two standard checks in response to 10 dS/m salinity stress (NaCl) and external Si source (1mM) during their seedling stage. All evaluated genotypes showed an evident decrease in biomass and chlorophyll content under salinity stress, while reporting an enhances in Si accumulation, Na+/K+ ratio, proline, electrolyte leakage, lipid peroxidation, hydrogen peroxide, and antioxidant activities. The external Si supplementation significantly improved rice tolerance to salinity through increased Si content, low Na+/K+ ratio, better osmolyte production, reduced membrane permeability, and improved antioxidant enzyme activities. Multivariate factor analysis with principal component factor statistically correlates and visualizes silicon accumulation with salt tolerance indices. The Hierarchical clustering in rice obtained based on the study of salt tolerance indices, distinguishes genotypes with different treatments into three clusters. In conclusion, the clustering grouped salt-tolerant Var.Lunishree and salt stress high silicon accumulating Var.Swarna together validates silicon mitigating effect on salinity in rice.

scholarly journals Genetic diversity analysis of advanced rice lines for salt tolerance using SSR markers

2021 ◽  
Vol 7 (2) ◽  
pp. 214-221
Author(s):  
Tanoy Singha ◽  
Md Asif Mahamud ◽  
Shahin Imran ◽  
Newton Chandra Paul ◽  
Md Najmol Hoque ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Salt Tolerance ◽  
Ssr Markers ◽  
Genetic Similarity ◽  
Potential Role ◽  
Diversity Analysis ◽  
Marker Assisted Breeding ◽  
Genetic Diversity Analysis ◽  
Ssr Analysis ◽  
Rice Genotypes

Twenty-two rice lines were used to evaluate salt tolerance at the Laboratory of Biotechnology Division of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Four SSR markers viz. AP3206f, RM1287, RM7075, and RM10793 were used to screen the germplasm for salt tolerance. SSR analysis revealed that the number of alleles per locus ranged from 3 to 5 with an average of 4.25 alleles per locus. Polymorphism Information Content (PIC) values ranged from 0.4762 (RM7075) to 0.7524 (AP3206f) with an average of 0.61 per locus. The highest genetic diversity (0.7810) was observed in loci AP3206f, and the lowest genetic diversity (0.5620) was observed in loci RM7075 with a mean diversity of 0.6663. The genotypes with genetic similarity clustered together in the dendrogram based on UPGMA method and we observed seven major clusters where cluster I contained most of the genotypes. Cluster I, II, III, IV, V, VI, and VII contained 6, 1, 2, 4, 4, 4 and 1 genotypes, respectively. These results revealed that marker AP3206f would be best in screening 22 rice genotypes followed by RM1287, RM7075, and RM10793 according to PIC values. These findings can have the potential role for further improvement of salinity tolerance rice genotypes through marker-assisted breeding. Asian J. Med. Biol. Res. 2021, 7 (2), 214-221

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