Detection of Salt Tolerant Rice Genotypes at the Seedling Stage Using Molecular Markers: A Genotypic Analysis

Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding. Int. J. Agril. Res. Innov. & Tech. 3 (1): 52-59, June, 2013 DOI: http://dx.doi.org/10.3329/ijarit.v3i1.16093

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Screening of Some Rice (Oryza sativa L.) Genotypes for Salinity Tolerance using Morphological and Molecular Markers

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2753 ◽

2019 ◽

Vol 16 (2) ◽

pp. 377-390

Author(s):

Shahabuddin Ahmed ◽

Touhidur Rahman Anik ◽

Ashraful Islam ◽

Imtiaz Uddin ◽

Mohammad S. Haque

Keyword(s):

Oryza Sativa L ◽

Rice Variety ◽

Dry Weight ◽

Crop Productivity ◽

Seedling Stage ◽

Group Method ◽

Salt Tolerant ◽

Pair Group ◽

Salinity Levels ◽

Rice Genotypes

Salinity is one of the major abiotic stresses, which adversely affects the crop productivity. Thirty rice genotypes of diverse origin including three salt tolerant check varieties, Binadhan-8, Binadhan-10 and Pokkali, were used to evaluate salt tolerance at seedling stage and to determine the genetic diversity using microsatellite markers. Salinity screening was done at the seedling stage using hydroponic system following IRRI standard protocol. Three salinity levels as 6dSm-1, 8dSm-1, and 10dSm-1 were used along with control. Data were recorded on root length, shoot length and dry weight and the genotypes were scored based on modified standard evaluation score (SES) for visual injury. Sixteen SSR markers were used to study the genetic variation within 30 rice genotypes. A total of 65 alleles with an average of 4.06 allele per locus were detected among 30 rice genotypes. The polymorphism information content (PIC) value ranged from 0.24 to 0.86 with an average of 0.51. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed four clusters. Among them cluster I identified 5 salt tolerant genotypes and cluster IV separated one tolerant and one moderately tolerant genotype. Based on SES evaluation and molecular analysis genotypes Balam, THDB, Q-31, Ab.Hai, BR-5, FR13A ware salt tolerant; Moulota, Super hybrid, Y-1281, Binadhan-16 were moderate salt tolerant. This information could be useful for selection of suitable genotypes for developing salt tolerant rice variety through molecular breeding.

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Marker-assisted Foreground Selection for Identification of Salt Tolerant Rice Genotypes

The Agriculturists ◽

10.3329/agric.v10i2.13128 ◽

2012 ◽

Vol 10 (2) ◽

pp. 1-8

Author(s):

MS Alam ◽

SN Begum ◽

MM Islam ◽

M Moniruzzaman ◽

MAK Patwary

Keyword(s):

Molecular Markers ◽

Life Cycle ◽

Marker Assisted Selection ◽

High Yield ◽

Salt Tolerant ◽

Short Life ◽

Foreground Selection ◽

Rice Genotypes ◽

Selection For ◽

Recipient Parent

ErratumIn the paper entitled Marker-assisted Foreground Selection for Identification of Salt Tolerant Rice Genotypes published in The Agriculturists 10(2): 1-8 (December 2012), the authors should be read as M. S. Alam, S. N. Begum, M. M. Islam, M. Moniruzzaman and M. A. K. Patwary instead of M. S. Alam, M. Salim, M. Moniruzzaman, J. A. Rashid and M. M. Islam (Authors changed on the online version on 13th June 2013).Marker Assisted Selection (MAS) technique was used to develop salt tolerant rice genotypes using molecular markers during June 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. FL-378 was identified as donor or male parent for saltol QTL and Binadhan-7 as recurrent or recipient parent which had high yield with short life cycle. Crossing was done between them and 10 F1 seeds were produced. PCR bands from all the 10F1 plants were scored as H represented heterozygous alleles for donor and recipient parent. Backcrossing was done to produce 105 BC1F1 seedlings. Foreground selection was performed with 72 BC1F1plants with a tightly linked salt tolerance marker RM21. Out of 72 plants, 33 plants were selected for RM21 showing H scores. The selected segregants were subjected to further recombinant and background selections at BC1F1 generation. These selected genotypess could be used for further foreground, recombinant and background selections with appropriate markers upto BC3 generation for the development of salt tolerant rice genotypes. DOI: http://dx.doi.org/10.3329/agric.v10i2.13128 The Agriculturists 2012; 10(2) 1-8

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Evaluation of Rice (Oryza sativa L.) Genotypes at Germination and Early Seedling Stage for Their Tolerance to Salinity

The Agriculturists ◽

10.3329/agric.v8i2.7578 ◽

1970 ◽

Vol 8 (2) ◽

pp. 57-65 ◽

Cited By ~ 5

Author(s):

MM Islam ◽

MA Karim

Keyword(s):

Oryza Sativa ◽

Oryza Sativa L ◽

Dry Weight ◽

Seedling Stage ◽

Saline Condition ◽

Salt Tolerant ◽

Early Seedling ◽

Rice Genotypes ◽

Tolerance To Salinity ◽

Cacl2 Solution

An experiment was conducted at the Laboratory of Agronomy Department, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur from July-June, 2009-2010 to evaluate the rice (Oryza sativa L.) genotypes for their tolerance to salinity. One hundred rice genotypes and two check cultivars (Pokkali as tolerant and IR29 as susceptible) were exposed to solution of Electrical Conductivity (EC) of 10, 15, and 20 dSm-1 (5:1 molar concentration of NaCl and CaCl2 solution) at germination and early seedling stage. Based on visual salt injury symptoms at 15 dSm-1, 13 genotypes were found fairly tolerant to salinity. However, among the 13 genotypes, only Patnai23 showed higher germination index and seedling relative dry weight than the check salt tolerant Pokkali at 15 dSm-1. Beside these, performance of Awned-1, Nonasail and Soloi was also well at this level. The genotypes Patnai23, Awned-1, Nonasail and Soloi showed the best performance under saline condition. Keywords: Rice genotypes; germination; salinity; tolerance DOI: 10.3329/agric.v8i2.7578 The Agriculturists 8(2): 57-65 (2010)

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Screening of Salinity Tolerance of Twenty Rice Genotypes at the Seedling Stage Through Hydroponic and Ssr Marker

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v8i1.53270 ◽

2021 ◽

Vol 8 (1) ◽

pp. 75-88

Author(s):

Sharif Ahmad Al Muti ◽

Md Shahidul Islam ◽

Md Monirul Islam ◽

Md Mahmud Al Noor ◽

SM Abdul Alim ◽

...

Keyword(s):

Abiotic Stresses ◽

Dry Matter ◽

Ssr Marker ◽

Shoot Length ◽

Seedling Stage ◽

Salt Tolerant ◽

The World ◽

Tolerant Variety ◽

Rice Genotypes ◽

Nei's Genetic Distance

Salinity is one of the major abiotic stresses which severely affect the production of crops across the world. In this experiment, we examined 20 rice genotypes of diverse origins and sources including few salt tolerant varieties (Binadhan-8, Binadhan-10, Pokkali and FL478) as check. The main objective of this study was to determine salt tolerance at seedling stage and to evaluate genetic variation using SSR markers. IRRI standard protocol was applied to screen out salinity among those varieties, at the glasshouse of Bangladesh Institute of Nuclear Agriculture, BAU campus, Mymensingh-2202. Shoot length, root length and total dry matter were recorded at 6dS/m, 8dS/m, 10dS/m and 12 dS/m salt stress levels. According to the morphological and molecular survey of 20 rice genotypes at the seedling stage it was evident that, Binadhan-8, Binadhan-10, Pokkali, FL478, IR64, IR4630, FR13A and Sadamota identified as salt tolerant whereas THDB, Moulata, MV-20, CPD-23, CPD-29, Pot-18, Pot-27 and Dudkalam those were found as susceptible, BRRI dhan67, Binadhan-17 and Binadhan-21 those were traced as highly susceptible. The highest Nei’s genetic distance value 1.0 was found in Moulata vs Sadamota and the lowest value 0.08 was observed in Binadhan-21 vs IR64. It will be used in future breeding program to develop a saline tolerant variety of rice. Res. Agric., Livest. Fish.8(1): 75-88, April 2021

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Variable Effects of Silicon on Salt Tolerant Indices in Rice Genotypes at Seedling Stage

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2020/v39i2830937 ◽

2020 ◽

pp. 43-55

Author(s):

Rinny Swain ◽

Surabhika Panda ◽

Gyana Ranjan Rout

Keyword(s):

Salt Tolerance ◽

Salinity Stress ◽

Antioxidant Activities ◽

Principal Component ◽

Seedling Stage ◽

Antioxidant Enzyme Activities ◽

Salt Tolerant ◽

Silicon Accumulation ◽

Rice Genotypes ◽

Tolerance Indices

Silicon (Si) is known to improve salt tolerance in rice. However, the correlation of silicon with different physiological and biochemical indices of salt tolerance is not properly understood. Two rice genotypes with different silicon accumulation ability were evaluated along with two standard checks in response to 10 dS/m salinity stress (NaCl) and external Si source (1mM) during their seedling stage. All evaluated genotypes showed an evident decrease in biomass and chlorophyll content under salinity stress, while reporting an enhances in Si accumulation, Na+/K+ ratio, proline, electrolyte leakage, lipid peroxidation, hydrogen peroxide, and antioxidant activities. The external Si supplementation significantly improved rice tolerance to salinity through increased Si content, low Na+/K+ ratio, better osmolyte production, reduced membrane permeability, and improved antioxidant enzyme activities. Multivariate factor analysis with principal component factor statistically correlates and visualizes silicon accumulation with salt tolerance indices. The Hierarchical clustering in rice obtained based on the study of salt tolerance indices, distinguishes genotypes with different treatments into three clusters. In conclusion, the clustering grouped salt-tolerant Var.Lunishree and salt stress high silicon accumulating Var.Swarna together validates silicon mitigating effect on salinity in rice.

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Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

Progressive Agriculture ◽

10.3329/pa.v19i2.16929 ◽

2013 ◽

Vol 19 (2) ◽

pp. 57-65

Author(s):

MH Kabir ◽

MM Islam ◽

SN Begum ◽

AC Manidas

Keyword(s):

Salt Tolerance ◽

Ssr Markers ◽

Salinity Tolerance ◽

Seedling Stage ◽

Phenotypic Screening ◽

Salt Tolerant ◽

Marker Assisted Breeding ◽

Hydroponic System ◽

Rice Landrace ◽

Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

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Effects of PEG induced water stress on growth and physiological responses of rice genotypes at seedling stage

Pakistan Journal of Botany ◽

10.30848/pjb2019-6(13) ◽

2019 ◽

Vol 51 (6) ◽

Author(s):

A. Shereen ◽

M.A. Khanzada ◽

M.A. Wahid Baloch ◽

Asma Asma ◽

M.U. Shirazi ◽

...

Keyword(s):

Water Stress ◽

Physiological Responses ◽

Seedling Stage ◽

Rice Genotypes

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Antioxidative and Metabolic Contribution to Salinity Stress Responses in Two Rapeseed Cultivars during the Early Seedling Stage

Antioxidants ◽

10.3390/antiox10081227 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1227

Author(s):

Ali Mahmoud El-Badri ◽

Maria Batool ◽

Ibrahim A. A. Mohamed ◽

Zongkai Wang ◽

Ahmed Khatab ◽

...

Keyword(s):

Salt Stress ◽

Salinity Stress ◽

Stress Responses ◽

Antioxidant Activities ◽

Tricarboxylic Acid Cycle ◽

Seedling Stage ◽

Plant Performance ◽

Salt Tolerant ◽

Brassica Napus L ◽

Early Seedling

Measuring metabolite patterns and antioxidant ability is vital to understanding the physiological and molecular responses of plants under salinity. A morphological analysis of five rapeseed cultivars showed that Yangyou 9 and Zhongshuang 11 were the most salt-tolerant and -sensitive, respectively. In Yangyou 9, the reactive oxygen species (ROS) level and malondialdehyde (MDA) content were minimized by the activation of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) for scavenging of over-accumulated ROS under salinity stress. Furthermore, Yangyou 9 showed a significantly higher positive correlation with photosynthetic pigments, osmolyte accumulation, and an adjusted Na+/K+ ratio to improve salt tolerance compared to Zhongshuang 11. Out of 332 compounds identified in the metabolic profile, 225 metabolites were filtrated according to p < 0.05, and 47 metabolites responded to salt stress within tolerant and sensitive cultivars during the studied time, whereas 16 and 9 metabolic compounds accumulated during 12 and 24 h, respectively, in Yangyou 9 after being sown in salt treatment, including fatty acids, amino acids, and flavonoids. These metabolites are relevant to metabolic pathways (amino acid, sucrose, flavonoid metabolism, and tricarboxylic acid cycle (TCA), which accumulated as a response to salinity stress. Thus, Yangyou 9, as a tolerant cultivar, showed improved antioxidant enzyme activity and higher metabolite accumulation, which enhances its tolerance against salinity. This work aids in elucidating the essential cellular metabolic changes in response to salt stress in rapeseed cultivars during seed germination. Meanwhile, the identified metabolites can act as biomarkers to characterize plant performance in breeding programs under salt stress. This comprehensive study of the metabolomics and antioxidant activities of Brassica napus L. during the early seedling stage is of great reference value for plant breeders to develop salt-tolerant rapeseed cultivars.

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