scholarly journals Screening of Some Rice (Oryza sativa L.) Genotypes for Salinity Tolerance using Morphological and Molecular Markers

2019 ◽  
Vol 16 (2) ◽  
pp. 377-390
Author(s):  
Shahabuddin Ahmed ◽  
Touhidur Rahman Anik ◽  
Ashraful Islam ◽  
Imtiaz Uddin ◽  
Mohammad S. Haque
Keyword(s):  
Oryza Sativa L ◽  
Rice Variety ◽  
Dry Weight ◽  
Crop Productivity ◽  
Seedling Stage ◽  
Group Method ◽  
Salt Tolerant ◽  
Pair Group ◽  
Salinity Levels ◽  
Rice Genotypes

Salinity is one of the major abiotic stresses, which adversely affects the crop productivity. Thirty rice genotypes of diverse origin including three salt tolerant check varieties, Binadhan-8, Binadhan-10 and Pokkali, were used to evaluate salt tolerance at seedling stage and to determine the genetic diversity using microsatellite markers. Salinity screening was done at the seedling stage using hydroponic system following IRRI standard protocol. Three salinity levels as 6dSm-1, 8dSm-1, and 10dSm-1 were used along with control. Data were recorded on root length, shoot length and dry weight and the genotypes were scored based on modified standard evaluation score (SES) for visual injury. Sixteen SSR markers were used to study the genetic variation within 30 rice genotypes. A total of 65 alleles with an average of 4.06 allele per locus were detected among 30 rice genotypes. The polymorphism information content (PIC) value ranged from 0.24 to 0.86 with an average of 0.51. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed four clusters. Among them cluster I identified 5 salt tolerant genotypes and cluster IV separated one tolerant and one moderately tolerant genotype. Based on SES evaluation and molecular analysis genotypes Balam, THDB, Q-31, Ab.Hai, BR-5, FR13A ware salt tolerant; Moulota, Super hybrid, Y-1281, Binadhan-16 were moderate salt tolerant. This information could be useful for selection of suitable genotypes for developing salt tolerant rice variety through molecular breeding.

scholarly journals Evaluation of Rice (Oryza sativa L.) Genotypes at Germination and Early Seedling Stage for Their Tolerance to Salinity

1970 ◽  
Vol 8 (2) ◽  
pp. 57-65 ◽  
Author(s):  
MM Islam ◽  
MA Karim
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Seedling Stage ◽  
Saline Condition ◽  
Salt Tolerant ◽  
Early Seedling ◽  
Rice Genotypes ◽  
Tolerance To Salinity ◽  
Cacl2 Solution

An experiment was conducted at the Laboratory of Agronomy Department, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur from July-June, 2009-2010 to evaluate the rice (Oryza sativa L.) genotypes for their tolerance to salinity. One hundred rice genotypes and two check cultivars (Pokkali as tolerant and IR29 as susceptible) were exposed to solution of Electrical Conductivity (EC) of 10, 15, and 20 dSm-1 (5:1 molar concentration of NaCl and CaCl2 solution) at germination and early seedling stage. Based on visual salt injury symptoms at 15 dSm-1, 13 genotypes were found fairly tolerant to salinity. However, among the 13 genotypes, only Patnai23 showed higher germination index and seedling relative dry weight than the check salt tolerant Pokkali at 15 dSm-1. Beside these, performance of Awned-1, Nonasail and Soloi was also well at this level. The genotypes Patnai23, Awned-1, Nonasail and Soloi showed the best performance under saline condition. Keywords: Rice genotypes; germination; salinity; tolerance DOI: 10.3329/agric.v8i2.7578   The Agriculturists 8(2): 57-65 (2010)   

scholarly journals Morpho-Molecular Characterization and Screening of Rice (Oryza Sativa L.) Genotypes for Salinity Tolerance at Seedling Stage

2021 ◽  
Vol 18 (2) ◽  
pp. 1-15
Author(s):  
SAA Muti ◽  
MI Hoque ◽  
MM Islam ◽  
MA Siddique ◽  
MS Islam
Keyword(s):  
Salt Tolerance ◽  
Molecular Characterization ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Seedling Stage ◽  
Group Method ◽  
Rice Varieties ◽  
Pair Group ◽  
Rice Genotypes ◽  
Near Future

Salinity is one of the major abiotic stresses which severely affect the production of crops across the world. Twenty rice genotypes of diverse origins were assessed to examine salt tolerance potentiality at seedling stage as well as to elucidate the genetic variation using SSR markers. Consequently, IRRI standard protocol was applied to screen out those varieties for tolerance at the glasshouse laboratory of Bangladesh Institute of Nuclear Agriculture, maintaining salt stress level at 6dS m-1, 8dS m-1, 10dS m-1 and 12 dS m-1. Data on morphological parameter were recorded as shoot length (cm), root length (cm) and total dry matter (TDM). For molecular characterization thirteen SSR markers were exploited to determine the genetic diversity and in this context, 53alleles were revealed while the mean number of alleles per locus was 4.The Polymorphism Information Content (PIC) value ranged from 0.37- 0.78 having an average of 0.58. The Un-weighted Pair Group Method with Arithmetic Mean (UPGMA) displayed three major clusters including some sub-clusters, where in Cluster 1, Binadhan- 8, Binadhan-10, Pokkali and FL478 were very close in their vicinity. Information obtained from this experiment, thus can be taken into account for stress breeding program and prospective genetic materials could be utilized for exploring the genetic architecture in favor of salt tolerance as well as for developing better salt tolerant rice varieties in near future. SAARC J. Agri., 18(2): 1-15 (2020)

scholarly journals Physiological Evaluation of Different Rice Genotypes to Two Salinity Level during Seedling Stage under Hydroponic System

2020 ◽  
pp. 42-56
Author(s):  
S. Lakshmi ◽  
V. Ravichandran ◽  
L. Arul ◽  
K. Krishna Surendar
Keyword(s):  
Salt Stress ◽  
Dry Weight ◽  
Seedling Stage ◽  
Salinity Level ◽  
Ion Concentration ◽  
Growth And Yield ◽  
Root Shoot Ratio ◽  
Ion Absorption ◽  
Early Seedling ◽  
Rice Genotypes

Hydroponics study was conducted to screen eight rice genotypes (CO 51, ADT 53, ADT 37, IR 64, CO 43, ASD 16, Pokkali; TRY 3) under salinity stress on early seedling stage. Two Saline treatments (75 and 100 mM NaCl) were given at 15 days old seedling; observations were recorded at 10 days after salt stress. Results showed that shoot length, root length, total fresh and dry weight, shoot and root fresh weight, shoot and root dry weight and root- shoot ratio were reduced under saline conditions compared to control. Na+ ion Concentration and Na+/ k+ ratio was higher in saline treatments than control. However, K+ ion absorption decreased with increasing salinity level. Electrolyte leakage and osmotic potential had increasing trend with increasing level of salinity. In this study, rice genotypes Pokkali, TRY 3 and CO 43 perform as tolerant; CO 51, ADT 53 and ASD 16 perform as moderately tolerant; ADT 37 is susceptible and IR 64 is highly susceptible. This type of study is required to develop salt tolerant genotypes at salt stress during seedling stage; to increase the growth and yield of rice there by satisfy the need of country’s requirement.

Salt Tolerance of Trifolium alexandrinum L. I. Comparison of the Salt Response of T. alexandrinum and T. pratense

1982 ◽  
Vol 9 (2) ◽  
pp. 221
Author(s):  
E Winter ◽  
A Lauchli
Keyword(s):  
Trifolium Pratense ◽  
Dry Weight ◽  
Solution Culture ◽  
Trifolium Alexandrinum ◽  
Salt Tolerant ◽  
Ion Distribution ◽  
Plant Parts ◽  
Salinity Levels ◽  
Survival Potential ◽  
Induced Changes

Trifolium alexandrinum and Trifolium pratense were grown in solution culture at salinity levels of 50, 100, 150 and 200 mM NaCl. Trifolium alexandrinum survived at all salt treatments. Salt-induced growth reductions of 30 and 47% occurred at 50 and 100 mM NaCl, respectively, mostly affecting stems. Plants still survived at 150 and 200 mM NaCl. This species can therefore be considered moderately salt tolerant. Trifolium pratense showed a low survival potential at salt treatments of 100 mM NaCl or higher, and the dry weight production of all plant parts was considerably affected at moderate salt levels. Thus T. alexandrinum is considered more salt tolerant than T. pratense. The distribution and contents of K+, Na+ and Cl- in both species indicate that T. pratense translocates Na+ and Cl- linearly with increasing salt treatment into stems and leaves, whereas low foliar Na+ and Cl- contents in T. alexandrinum suggest some mechanisms that control the ion distribution in the different plant parts. Salt-induced changes of the K+ and Ca2+ contents of the different plant parts of both species are discussed.

scholarly journals Evaluation of Rice Germplasm under Salt Stress at the Seedling Stage through SSR Markers

2013 ◽  
Vol 3 (1) ◽  
pp. 52-59 ◽  
Author(s):  
M Al-Amin ◽  
MM Islam ◽  
SN Begum ◽  
MS Alam ◽  
M Moniruzzaman ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Salinity Tolerance ◽  
Dry Matter ◽  
Growth Parameters ◽  
Seedling Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Genotypes ◽  
Salt Susceptible

Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding. Int. J. Agril. Res. Innov. & Tech. 3 (1): 52-59, June, 2013 DOI: http://dx.doi.org/10.3329/ijarit.v3i1.16093

scholarly journals Phosphorus and Zinc Fertilization Improve Zinc Biofortification in Grains and Straw of Coarse vs. Fine Rice Genotypes

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1155
Author(s):  
Amanullah ◽  
Inamullah ◽  
Mona S. Alwahibi ◽  
Mohamed Soliman Elshikh ◽  
Jawaher Alkahtani ◽  
...  
Keyword(s):  
Management Practices ◽  
Field Experiments ◽  
Negative Impact ◽  
Oryza Sativa L ◽  
Crop Productivity ◽  
Continuous Cropping ◽  
Rice Varieties ◽  
Rice Grains ◽  
Rice Genotypes ◽  
The Impact

Continuous cropping of rice (Oryza sativa L.) and wheat (Triticum aestivum L.) deplete soil fertility and reduce crop productivity as well as zinc (Zn) concentrations in rice grains and straw. Low Zn concentrations in rice grains have a negative impact on human health, while low Zn concertation in rice straw creates a nutritional problem for animals. The current high yielding rice varieties and hybrids remove large quantities of Zn from the soils, lowering the residual concentrations of soil Zn for the subsequent crop (e.g., wheat). Field experiments were conducted on farmers field in Malakand with the objective to evaluate the impact of various combinations of phosphorus (0, 40, 80, and 120 kg ha−1) and Zn levels (0, 5, 10, and 15 kg ha−1) on biofortification of Zn in grains and straw of rice genotypes [fine (Bamati-385) vs. coarse (Fakhre-e-Malakand and Pukhraj)]. The results revealed that Zn biofortification in rice genotypes increased with the integrated use of both nutrients (P + Zn) when applied at higher rates (80 and 120 kg P ha−1, and 10 and 15 kg Zn ha−1, respectively). The biofortification of Zn in both grains and straw was higher in the coarse than fine rice genotypes (Pukhraj > Fakhre-e-Malakand > Basmati-385). It was concluded from this study that the application of higher P and Zn levels increased Zn contents in rice parts (grains and straw) under the rice-wheat system. We also concluded from this study that Zn concentrations in rice grains and straw are influenced by plant genetic factors and Zn management practices.

Differential expression of salt-responsive genes to salinity stress in salt-tolerant and salt-sensitive rice (Oryza sativa L.) at seedling stage

PROTOPLASMA ◽  
2018 ◽  
Vol 255 (6) ◽  
pp. 1667-1681 ◽  
Author(s):  
Vijayata Singh ◽  
Ajit Pal Singh ◽  
Jyoti Bhadoria ◽  
Jitender Giri ◽  
Jogendra Singh ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Differential Expression ◽  
Salinity Stress ◽  
Oryza Sativa L ◽  
Seedling Stage ◽  
Salt Tolerant

scholarly journals Evaluation of Rice (Oryza sativa L.) Genotypes for Drought Tolerance at Germination and Early Seedling Stage

2018 ◽  
Vol 16 (1) ◽  
pp. 44-54 ◽  
Author(s):  
M M Islam ◽  
E Kayesh ◽  
E Zaman ◽  
T A Urmi ◽  
M M Haque
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Evaluation System ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Seedling Height ◽  
Early Seedling ◽  
Rice Genotypes

Drought stress is a major constraint to the production and yield stability of crops. Rice (Oryza sativa L.) is considered as a drought-sensitive crop species. Within this species, there are considerable varietal differences in sensitivity to this environmental stress. An experiment was conducted at the laboratory of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during April to September 2016 to evaluate 100 rice genotypes for drought tolerance during germination and early seedling growth stage. The genotypes were tested against five levels of drought stress imposed by Polyethylene glycol 6000 (PEG-6000) @ 0, 5, 10, 15 and 20%. The experiment was laid out in a complete randomized design with four replications. The results showed that with increasing water stress, germination in all the genotypes decreased from 95.8% in control to 6.6 % in highest stress (20% PEG) level. Seedling height and dry weight also decreased in all rice genotypes with the increase in water stress level. Based on Standard Evaluation System (SES),18 genotypes were selected primarily. Among the 18 genotypes, Ratoil and Chinisakkar showed higher germination index, relative seedling height and relative seedling dry weight than the check drought tolerant BRRI dhan43 at 20% PEG. Beside these, performance of Kumridhan, Pusur and Somondori was also well at this level compared to BRRI dhan43. The genotypes Ratoil, Chinisakkar, Kumridhan, Pusur and Somondori showed the best performance under drought condition. The Agriculturists 2018; 16(1) 44-54

scholarly journals Priming Benih Padi (Oryza sativa L.) dengan Zn untuk Meningkatkan Vigor Bibit pada Cekaman Terendam

2016 ◽  
Vol 44 (1) ◽  
pp. 8
Author(s):  
Sulaiman Sulaiman ◽  
Rujito Agus Suwignyo ◽  
Mery Hasmeda ◽  
Andi Wijaya
Keyword(s):  
Oryza Sativa L ◽  
Block Design ◽  
Dry Weight ◽  
Seed Priming ◽  
Rice Seed ◽  
Seedling Vigor ◽  
Randomized Block Design ◽  
Submergence Stress ◽  
Soil Media ◽  
Rice Genotypes

<br />Seed priming has been widely used to improve seedling vigor of several crops  such as corn, wheat, and rice. Seed priming with zinc to increase seedling tolerance on submergence stress had never been evaluated. The objective of this research was to identify agronomical and physiological characteristics of several rice genotypes during seedling stage at submergence stress following zinc priming treatment. This research used factorial randomized block design with three replications. The first factor was rice genotypes and second factor was zinc solution of ZnSO4.7H2O. Seeds were soaked with Zn solution at different concentrations namely 0, 2.5, dan 5.0 mM respectively for 24 hours. After that the seeds were germinated on soil media in side the plastic box. Fourteen days-old seedling were submerged in water for 5 days and then return to normal condition for recovery. Parameters being observed included seedling dry weight, shoot carbohydrate, and leaves chlorophyl which were measured after 10 days of recovery. Seedling height was measured once a week from 2nd to 4th week. Results showed that intolerant genotypes to submergence stress grew faster but seedling dry weight, shoot carbohydrate and leaves chlorophyll content were lower compared to those tolerant genotypes. Local and national varieties which were not tolerant to submergence stress could be improved by priming treatment with 5.0mM zinc.<br /><br />Keywords: abiotic-stress, seeds- priming <br /><br />

scholarly journals Impact of elevated temperature on rice productivity: A case of Lalitpur, Nepal

2016 ◽  
Vol 4 ◽  
pp. 19-22
Author(s):  
Ambika Ghimire ◽  
Yubak Dhoj G.C. ◽  
Binod Baniya
Keyword(s):  
Elevated Temperature ◽  
Oryza Sativa L ◽  
Abiotic Factors ◽  
Rice Variety ◽  
Soil Nutrient ◽  
Growth Period ◽  
Crop Productivity ◽  
Straw Yield ◽  
Important Crop ◽  
The Impact

Rice (Oryza sativa L.) is the second most important crop in the world after wheat and also the most important crop in Nepal. The production of rice is influenced by various biotic and abiotic factors. Temperature is the major constraint for the crop yield. The present experiment was conducted to study the impact of temperature on straw and crop productivity from June to October 2014. The experiment was conducted under temperature control chamber, in which temperature was elevated from the ambient level by 2ºC and 3ºC for the entire crop growth period. Grain and straw yield was measured using electronic weighing machine. Maturity of grain was 10 and 7 days earlier at elevated temperature by 3ºC and 2ºC respectively. Under similar condition of water depth, plant spacing, rice variety and soil nutrient, rise in temperature up to 2ºC is favorable for rice straw yield and crop productivity. Yield loss under elevated temperature by 3ºC is due to floret sterility. Further research on temperature resistance rice variety is necessary.

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