scholarly journals Orcein and DAPI-stained karyotype analysis of Alocasia macrorrhizos (L.) G. Don

2021 ◽  
Vol 45 (1) ◽  
pp. 27-35
Author(s):  
Ashma Ahmed Warasy
Keyword(s):  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Asymmetry Index ◽  
Lower Amount ◽  
Interphase Nuclei ◽  
Karyotype Asymmetry ◽  
Karyotype Analyses ◽  
Dapi Banding ◽  
Gradient Type ◽  
Chromosomal Length

Karyotype analyses are required for the identification, characterization, and genetic improvement of any organism. Alocasia macrorrhizos (L.) G. Don. was investigated cytogenetically to determine the karyotypic features. Complex chromocenter type, of interphase nuclei, and gradient type of prophase chromosomes were found in this study. Alocasia macrorrhizos was found to possesses 2n=28 chromosomes. The total length of the 2n chromosome complement was recorded as 98.83±1.39 μm. The range of chromosomal length was 2.50±0.10-4.70±0.10 μm. A gradual decrease in chromosomal length was observed. The total form (TF%) value was found to be 43.58%, Karyotype symmetry index (Syi %) was 77.00 % and karyotype asymmetry index (AsK %) was 56.66%. The centromeric formula was 18m+4sm+2ac, representing asymmetric karyotype. In DAPI banding, the 1.48% positive banded region indicates the lower amount of AT rich repeats in this material. Therefore, Alocasia macrorrhizos could be authentically characterized through karyotype analysis. J. Bangladesh Acad. Sci. 45(1); 27-35: June 2021

scholarly journals Comparative karyomorphological analysis of two varieties of Brassica campestris from Bangladesh

2021 ◽  
Vol 9 (1-2) ◽  
pp. 69-77
Author(s):  
Ashma Ahmed Warasy
Keyword(s):  
Brassica Campestris ◽  
Crop Improvement ◽  
Chromosome Complement ◽  
Relative Length ◽  
Asymmetry Index ◽  
Continuous Type ◽  
Karyotype Asymmetry ◽  
Improvement Programme ◽  
Similar Range ◽  
Chromosomal Length

Two varieties of the oilseed plant viz. Brassica campestris var. Tori-7 and Brassica campestris var. BARI Sarisha-9 were karyomorphologically investigated following orcein staining. Presence of “Round Pro-chromosome Type” of interphase nuclei and “Continuous Type” of prophase chromosomes indicating that both Tori-7 and BARI Sarisha-9 were originated from common ancestor. Moreover, both the varieties were found to possess 2n=20 chromosomes with almost similar range of length i.e.; 0.90±0.04-2.51±0.05μm for Tori-7 and 0.99±0.06-2.60±0.06μm for BARI Sharisha-9. The total length of 2n chromosome complement was recorded as 29.61±0.76μm for Tori-7 and 29.75±0.80μm for BARI Sharisha- 9 where a slight gradual decrease of chromosomal length was observed in both cases. Average chromosomal length, range of individual chromosomal length, relative length and centromeric index of both varieties were more or less similar. Karyotype symmetry index and Karyotype asymmetry index indicating the symmetric nature of both varieties. All metacentric chromosomes were found in Tori-7 representing strictly symmetric karyotype. Beside metacentric, 2 sub-metacentric chromosomes were found in BARI Sharisha-9 representing almost symmetric karyotype. Thus the two varieties of Brassica campestris -were plants of primitive nature. However, BARI Sharisha-9 can be considered comparatively advanced than Tori-7 based on different cytogenetical features. Therefore, the compilation of these karyomorphological information will be useful for authentic identification and characterization of the two varieties of Brassica campestris which are very basic and important information for breeding in crop improvement programme. Jahangirnagar University J. Biol. Sci. 9(1 & 2): 69-77, 2020 (June & December)

scholarly journals Karyotype analysis with orcein and CMA in two species of Alocasia (Schott) G. Don (Araceae)

1970 ◽  
Vol 40 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Syeda Sharmeen Sultana ◽  
Hosne Ara ◽  
Sheikh Shamimul Alam
Keyword(s):  
Chromosome Number ◽  
Key Words ◽  
Karyotype Analysis ◽  
Chromosome Complement ◽  
Taxonomic Status ◽  
The Other ◽  
First Report ◽  
Total Length ◽  
Internet Information ◽  
Chromosomal Length

Alocasia fallax Schott and A. odora (Roxb.) Koch (Araceae) were investigated cytogenetically to confirm their taxonomic status. There is no report of 2n chromosome number for A. fallax in the available literature and internet information. Therefore the 2n chromosome number (2n = 28) found in this study is probably the first report for A. fallax. Alocasia odora showed exactly double 2n chromosome number (2n = 56) from A. fallax. In addition to chromosome number, the other karyotypic features of A. odora were exactly double for that of A. fallax. The centromeric formulae of A. fallax was 24 m + 4 sm whereas it is just double in A. odora. Total length of 2n chromosome complement of A. odora (62.58 μm) was almost double to A. fallax. The range of chromosomal length of the two species was almost same. Moreover, A. odora plant is much taller than A. fallax. All of these data suggests that A. odora might be an autotetraploid of A. fallax which in course of evolution had undergone some changes in GC-rich repeats. Key words: Alocasia; CMA; Karyotype analysis DOI: http://dx.doi.org/10.3329/bjb.v40i1.7998 Bangladesh J. Bot. 40(1): 53-56, 2011 (June)

scholarly journals Cytotaxonomy of four Floral Variants of Impatiens Balsamina L.

2019 ◽  
Vol 43 (1) ◽  
pp. 1-9
Author(s):  
Chandan Kumar Dash ◽  
Md Harun Ar Rashid ◽  
Syeda Sharmeen Sultana ◽  
M Oliur Rahman
Keyword(s):  
Chromosome Length ◽  
Interphase Nuclei ◽  
Symmetry Index ◽  
Continuous Type ◽  
Impatiens Balsamina ◽  
Total Chromosome Length ◽  
Karyotype Asymmetry ◽  
Gradient Type ◽  
Academy Of Sciences ◽  
Orcein Staining

Karyomorphological characters were investigated in four floral variants of Impatiens balsamina L. viz. white, pink, violet and red for proper characterization and inferring relationships among them. All four floral variants of I. balsamina were found to possess 2n = 14 chromosomes, and ‘Simple Chromocenter Type’ of interphase nuclei was observed in all variants with many small heterochromatin blocks after orcein staining. ‘Continuous Type’ of prophase chromosomes was found in red variant, whereas both ‘Continuous and Gradient Type’ were found in other three variants. The red variant could easily be distinguished from the remaining three variants by total chromosome length, centromeric formula, average chromosome length, total form value, and karyotype asymmetry plus symmetry index. Based on karyomorphology, the white, pink and violet variants of I. balsamina were found very closely related and constituted with similar genome, whereas red variant was found to possess slightly modified genome and distinct from other three floral variants. Journal of Bangladesh Academy of Sciences, Vol. 43, No. 1, 1-9, 2019

Aspidistra revoluta, a new species from limestone areas in Chongqing, China

Phytotaxa ◽  
2016 ◽  
Vol 257 (3) ◽  
pp. 280 ◽  
Author(s):  
Hao Zhou ◽  
Si-rong Yi ◽  
Qi Gao ◽  
Jie Huang ◽  
Yu-jing Wei
Keyword(s):  
New Species ◽  
Chromosome Number ◽  
Average Length ◽  
Chromosome Complement ◽  
Pollen Grains ◽  
The Other ◽  
Morphological Comparison ◽  
Chongqing Municipality ◽  
Karyotype Asymmetry ◽  
A New Species

Aspidistra revoluta (Asparagaceae) is described and illustrated as a new species from limestone areas in southern Chongqing Municipality, China. The new species can be distinguished from the other Aspidistra species by its unique umbrella-like pistil with large revolute stigma lobes that bent downwards and touch the base of the perigone. A detailed morphological comparison among A. revoluta, A. nanchuanensis and A. carnosa is provided. The pollen grains of A. revoluta are subspherical and inaperturate, with verrucous exine. The chromosome number is 2n = 38, and the karyotype is formulated as 2n = 22m + 6sm + 10st. The average length of chromosome complement is 4.50 μm, and the karyotype asymmetry indexes A1 and A2 are respectively 0.37±0.03 and 0.49±0.01.

scholarly journals Karyotype analysis and chromosome number for two Cirsium taxa (Asteraceae) in Iran

Turczaninowia ◽  
2021 ◽  
Vol 24 (1) ◽  
pp. 83-88
Author(s):  
Zohreh Babaee ◽  
Maryam Norouzi ◽  
Samaneh Mosaferi ◽  
Maryam Keshavarzi
Keyword(s):  
Chromosome Numbers ◽  
Mitotic Chromosome ◽  
Karyotype Analysis ◽  
Chromosome Length ◽  
B Chromosomes ◽  
Chromosome Type ◽  
Population Total ◽  
Ploidy Levels ◽  
Class B ◽  
Karyotype Analyses

Cirsium Mill. contains more than 250 species in the world mainly distributed in the Northern hemisphere. Different chromosome numbers with different ploidy levels were reported in this genus. In this study, karyotype details and chromosome numbers were established for two Cirsium taxa in Iran. C. ciliatum subsp. szovitsii and C. echinus had the mitotic chromosome numbers of 2n = 2x = 34. Karyotype analyses showed that chromosomes were generally metacentric and sub-metacentric. In C. echinus, Lowshan population had the longest chromosome (19.10 µm) and Heyran Canyon population (4.73 µm) the shortest one while in C. ciliatum, the longest chromosome was observed in Urmia to Salmas population (14.67 µm) and the shortest one (4.71 µm) in Doshanlu population. Total haploid chromosome length ranged from 275.29 to 376.42 µm in populations studied. Both taxa were grouped in 2B class. B-chromosomes were recorded for two taxa studied too. Chromosome type, mitotic chromosome numbers and occurrence of B-chromosomes were in agreement with previous results (Albers, Pröbsting, 1998; Lövkvist, Hultgård, 1999; Yüksel et al., 2013; Yildiz et al., 2016).

scholarly journals Karyotype analysis with orcein and CMA from leaf base cells of Rauvolfia serpentina Benth. et Kurz

1970 ◽  
Vol 38 (1) ◽  
pp. 111-113 ◽  
Author(s):  
Syeda Sharmeen Sultana ◽  
ANM Rubaiyath Bin Rahman ◽  
Sheikh Shamimul Alam
Keyword(s):  
Key Words ◽  
Karyotype Analysis ◽  
Chromosome Length ◽  
Entire Length ◽  
Leaf Base ◽  
Interphase Nuclei ◽  
Rich Region ◽  
Rauvolfia Serpentina ◽  
Fluorescent Banding ◽  
Orcein Staining

Many prominent darkly stained heterochromatic blocks were found in the interphase nuclei of Rauvolfia serpentina following orcein staining. The prophase chromosomes of this species became stained homogeneously throughout the entire length. This species was found to possess 2n = 20 metacentric chromosomes revealing symmetric karyotype but if the chromosome length (6.67 - 3.17 μm) is considered, it indicates asymmetric karyotype. Total GC-rich region was 15.62% of the total chromatin length. Eight CMA-positive bands on different locus revealed the accumulation of GC-rich repeats. The two entirely CMA-banded chromosomes were so unique that could be used as marker for this species. Key words: Karyotype; Rauvolfia serpentina; Fluorescent banding DOI: 10.3329/bjb.v38i1.5133 Bangladesh J. Bot. 38(1): 55-63, 2009 (June)

Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISH

2001 ◽  
Vol 102 (6-7) ◽  
pp. 810-814 ◽  
Author(s):  
R. Nakamura ◽  
S. Kitamura ◽  
M. Inoue ◽  
N. Ohmido ◽  
K. Fukui
Keyword(s):  
Karyotype Analysis ◽  
Dapi Banding ◽  
Rdna Fish

scholarly journals COMPARATIVE KARYOMORPHOLOGICAL STUDIES OF TWO SPECIES OF CRINUM

2021 ◽  
Vol 9 (02) ◽  
pp. 787-792
Author(s):  
Shahla Firdaus ◽  
◽  
Kamini Kumar ◽  
Keyword(s):  
Asymmetry Index ◽  
Gradient Index ◽  
Symmetry Index ◽  
Index Form ◽  
Karyotype Asymmetry ◽  
The Family ◽  
Crinum Asiaticum

Crinum asiaticum L. and Crinum latifolium L. are members of the family Amaryllidaceae. Karyomorphological studies of these two species had been performed by using different quantitative and qualitative parameters to know the karyotype asymmetry, which defines the advanced and primitive nature of these two species. The study revealed that Crinum asiaticum L. and Crinum latifolium L. are diploid with 2n=22 chromosomes. based on Total Chromatin Length, Arm Ratio, Gradient Index, Form Percentage, Total Form Percentage, Karyotype Asymmetry Index, Symmetry Index, and Stebbins classification, Crinum asiaticum L. is more advanced than Crinum latifolium L.

scholarly journals Cytogenetics of Pennisetum purpureum Schumack x Pennisetum glaucum L. hybrids and their parents

2003 ◽  
Vol 27 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Sandro Barbosa ◽  
Lisete Chamma Davide ◽  
Antônio Vander Pereira
Keyword(s):  
Interspecific Hybrids ◽  
Pennisetum Glaucum ◽  
Chromosome Complement ◽  
Chromosome Morphology ◽  
Pennisetum Purpureum ◽  
Germplasm Bank ◽  
Cytogenetic Studies ◽  
Length Data ◽  
Chromosomal Length

Cytogenetic studies were carried out on five accesses of interspecific hybrids of Pennisetum purpureum and Pennisetum glaucum and their parentals, which are part of the Forage Germplasm Bank of the "Centro Nacional de Pesquisa de Gado de Leite" of EMBRAPA, Brazil. The mitotic cytogenetic analysis, performed using stain by Schiff reactive, confirmed the chromosome number of 2n = 14, 28 and 21 for the accesses of P. glaucum, P. purpureum and their hybrids, respectively. For each access, descriptions of the chromosome complement and idiograms are presented which allowed to establish the karyotypic relationships between the hybrids and their parentals. Based on the total chromosomal length data it was possible to establish the karyotype classification of the accesses concerning symmetry. While the karyotype of P. glaucum accesses was considered symmetric, hybrids presented asymmetric karyotypes. For P. purpureum, two accesses (BAGs 63 and 75) showed symmetric karyotype and three (BAGs 54, 65 and 91) were asymmetric. For the interspecific hybrids it was possible to infer the origin of some parental chromosomes based on the total chromosomal length measurements, satellite presence and chromosome morphology.

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