scholarly journals PCR-based survey of vector-borne pathogens in dogs in Dhaka, Bangladesh

2013 ◽  
Vol 10 (2) ◽  
pp. 249-254 ◽  
Author(s):  
MH Talukder ◽  
A Matsuu ◽  
A Iguchi ◽  
BC Roy ◽  
N Nishii ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
18S Rrna ◽  
Babesia Gibsoni ◽  
Specific Primers ◽  
Rrna Sequence ◽  
Blood Samples ◽  
Vector Borne ◽  
Candidatus Mycoplasma Turicensis ◽  
Mycoplasma Spp

To identify tick-borne pathogens in blood samples from 68 dogs were examined for Babesia, Anaplasma/Ehrichia, Mycoplasma, and Hepatozoon spp. by using a molecular tool. The detection of DNA was performed using specific primers for partial 16S rRNA or 18S rRNA sequence, and amplicon sequences were analyzed. Our investigation detected Babesia gibsoni (26 dogs) and Anaplasma sp. AnHl446 (2 dogs), Mycoplasma haemocanis (27 dogs), and an unknown Mycoplasma spp were detected (2 dogs), which was most closely related to Candidatus Mycoplasma turicensis, on the basis of phylogenetic analysis. Hepatozoon DNA could not be detected in this study.DOI: http://dx.doi.org/10.3329/jbau.v10i2.14915 J. Bangladesh Agril. Univ. 10(2): 249-253, 2012

scholarly journals The Termite Group I Phylum Is Highly Diverse and Widespread in the Environment

2007 ◽  
Vol 73 (20) ◽  
pp. 6682-6685 ◽  
Author(s):  
Daniel P. R. Herlemann ◽  
Oliver Geissinger ◽  
Andreas Brune
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Specific Primers ◽  
Environmental Distribution ◽  
Data Set ◽  
Group I

ABSTRACT The bacterial candidate phylum Termite Group I (TG-1) presently consists mostly of “Endomicrobia,” which are endosymbionts of flagellate protists occurring exclusively in the hindguts of termites and wood-feeding cockroaches. Here, we show that public databases contain many, mostly undocumented 16S rRNA gene sequences from other habitats that are affiliated with the TG-1 phylum but are only distantly related to “Endomicrobia.” Phylogenetic analysis of the expanded data set revealed several diverse and deeply branching lineages comprising clones from many different habitats. In addition, we designed specific primers to explore the diversity and environmental distribution of bacteria in the TG-1 phylum.

Phylogenetic analysis of the genus Anaplasma in Southwestern China based on 16S rRNA sequence

2010 ◽  
Vol 89 (2) ◽  
pp. 262-265 ◽  
Author(s):  
Zuoyong Zhou ◽  
Kui Nie ◽  
Cheng Tang ◽  
Zhiying Wang ◽  
Rongqiong Zhou ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Southwestern China ◽  
Rrna Sequence ◽  
16S Rrna Sequence

scholarly journals First Molecular Detection of Babesia gibsoni in Stray Dogs from Thailand

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 639
Author(s):  
Thom Do ◽  
Ruttayaporn Ngasaman ◽  
Vannarat Saechan ◽  
Opal Pitaksakulrat ◽  
Mingming Liu ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Babesia Gibsoni ◽  
Its1 Region ◽  
Stray Dogs ◽  
Southern Thailand ◽  
Vector Borne

In southern Thailand, the increasingly growing population of stray dogs is a concern to public health and environmental safety because of the lack of medical attention and control. More importantly, these animals are considered reservoirs for many zoonotic pathogens. The objective of this study was to molecularly detect canine vector-borne pathogens, and to perform genetic characterization of Babesia gibsoni present in stray dogs from southern Thailand. Blood samples were collected from 174 stray dogs in two provinces (Songkhla and Narathiwat) in southern Thailand. PCR analyses were executed using specific primers based on the Babesia spp. 18S rRNA gene, Babesia gibsoni Internal transcribed spacer 1 (ITS1) region, Ehrlichia canis citrate synthase (gltA) gene, Hepatozoon spp. 18S rRNA gene and Anaplasma platys heat shock protein (groEL) gene. The most common canine vector-borne pathogen found infecting stray dogs in this study was Hepatozoon canis (24.7%) followed by A. platys (14.9%), Babesia vogeli (8.0%), B. gibsoni (6.3%), and E. canis (1.72%). Concurrent infection with more than one pathogen occurred in 72 cases. Phylogenetic analysis based on the ITS1 region and 18S rRNA gene revealed that the B. gibsoni isolates from this study shared a large proportion of their identities with each other and with other reported B. gibsoni genotypes from Asia. This study highlights the molecular detection of B. gibsoni in dogs in Thailand for the first time and presents the genetic characterization by sequencing the ITS1 region and 18S rRNA gene of B. gibsoni from Thailand. Follow-up studies are needed to elucidate the origin, distribution, and vectors of B. gibsoni parasites circulating in dogs in Thailand, as well as to determine to what extent dogs are important reservoir hosts for zoonotic canine vector-borne disease infection in the studied area.

scholarly journals Haemotropic Mycoplasma species in pet cats in Latvia: a study, phylogenetic analysis and clinical case report

2021 ◽  
Vol 7 (2) ◽  
pp. 205511692110280
Author(s):  
Inese Berzina ◽  
Valentina Capligina ◽  
Agne Namina ◽  
Alina Visocka ◽  
Renate Ranka
Keyword(s):  
Phylogenetic Analysis ◽  
Clinical Case ◽  
Mycoplasma Species ◽  
Baltic Region ◽  
Blood Samples ◽  
Northern European ◽  
Published Report ◽  
The Baltic ◽  
First Time ◽  
Candidatus Mycoplasma Turicensis

Objectives The aim of this study was to evaluate whether haemotropic Mycoplasma species are detected in pet cats in Latvia, to perform a phylogenetic analysis of the detected pathogens and to report a clinical case of feline infectious anaemia. Methods Peripheral blood samples (n = 125) from pet cats were submitted; 99 samples were adequate to test for the presence of Mycoplasma species DNA by nested PCR. A clinical case was added in the later stages of the study. Positive isolates were subjected to phylogenetic analysis. Results The prevalence of ‘ Candidatus Mycoplasma haemominutum’ was 15% (n = 15/99), that of Mycoplasma haemofelis was 5% (5/99) and that of ‘ Candidatus Mycoplasma turicensis’ was 2% (n = 2/99). Cases of coinfection included ‘ Candidatus M haemominutum’ + M haemofelis (4%; n = 4/99) and ‘ Candidatus M haemominutum’ + ‘ Candidatus M turicensis’ (1%; n = 1/99). This is the first published report of M haemofelis infection in the Baltic states. Two different ‘ Candidatus M turicensis’ isolates were discovered after phylogenetic analysis. Conclusions and relevance This report is the first of an autochthonous feline infectious anaemia case in the Baltic region. The prevalence of Mycoplasma species was similar to that in other northern European countries. Phylogenetic analysis revealed variability of the isolates; one of the ‘ Candidatus M turicensis’ genotypes was detected for the first time in Europe.

scholarly journals Molecular Characterization of “Candidatus Parilichlamydia carangidicola,” a Novel Chlamydia-Like Epitheliocystis Agent in Yellowtail Kingfish, Seriola lalandi (Valenciennes), and the Proposal of a New Family, “Candidatus Parilichlamydiaceae” fam. nov. (Order Chlamydiales)

2012 ◽  
Vol 79 (5) ◽  
pp. 1590-1597 ◽  
Author(s):  
M. C. Stride ◽  
A. Polkinghorne ◽  
T. L. Miller ◽  
J. M. Groff ◽  
S. E. LaPatra ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Arctic Charr ◽  
Rrna Sequence ◽  
Content Type ◽  
New Family ◽  
Gill Lamellae ◽  
Yellowtail Kingfish ◽  
Seriola Lalandi

ABSTRACTThree cohorts of farmed yellowtail kingfish (Seriola lalandi) from South Australia were examined forChlamydia-like organisms associated with epitheliocystis. To characterize the bacteria, 38 gill samples were processed for histopathology, electron microscopy, and 16S rRNA amplification, sequencing, and phylogenetic analysis. Microscopically, the presence of membrane-enclosed cysts was observed within the gill lamellae. Also observed was hyperplasia of the epithelial cells with cytoplasmic vacuolization and fusion of the gill lamellae. Transmission electron microscopy revealed morphological features of the reticulate and intermediate bodies typical of members of the orderChlamydiales. A novel 1,393-bp 16S chlamydial rRNA sequence was amplified from gill DNA extracted from fish in all cohorts over a 3-year period that corresponded to the 16S rRNA sequence amplified directly from laser-dissected cysts. This sequence was only 87% similar to the reported “CandidatusPiscichlamydia salmonis” (AY462244) from Atlantic salmon and Arctic charr. Phylogenetic analysis of this sequence against 35ChlamydiaandChlamydia-like bacteria revealed that this novel bacterium belongs to an undescribed family lineage in the orderChlamydiales. Based on these observations, we propose this bacterium of yellowtail kingfish be known as “CandidatusParilichlamydia carangidicola” and that the new family be known as “CandidatusParilichlamydiaceae.”

scholarly journals Taxonomy and antimicrobial activity of streptosporangium strain SG163 isolated from a Saharan soil

2019 ◽  
Vol 20 (1) ◽  
pp. 35-54
Author(s):  
K. Bouti ◽  
◽  
H. Boudjella ◽  
N. Bouras ◽  
A. Zitouni ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Culture Media ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Saharan Soil ◽  
Type Strains

n a continuing search fornew antimicrobial products from actinobacteriacollected in Algerian Saharan soils, an isolateof actinobacteria,designated Sg163, was selected for its interesting antimicrobial activity.The isolate wasidentified to the genus Streptosporangium by phenotypicand molecular criteria. The cultural and physiological characterizations as well as phylogenetic analysis indicated that the isolate was different from known members of the genus Streptosporangium. Analysis of the 16S rRNA sequence showed 97.09to 98.27% similarity with those of Streptosporangium type strains. The strainSg163 produced antifungal and antibacterial activities on several culture media. The highest antimicrobial activities were obtained in ISP2 medium. Three active products C1, C2 and C3 with both antifungal and antibacterial activities were isolated and purified by chromatographic methodswith C2, as the major compound. The data of the infraredspectroscopy andthe chemical revelations, suggested that the active molecules were glycosylatedaromaticcompounds.

scholarly journals Detection and genetic characterization of "Candidatus Mycoplasma haemomacaque" infection among long-tailed macaques (Macaca fascicularis) in Thailand using broad-range nested polymerase chain reaction assay

2021 ◽  
Vol 14 (4) ◽  
pp. 943-948
Author(s):  
Wanat Sricharern ◽  
Supakarn Kaewchot ◽  
Sarawan Kaewmongkol ◽  
Natnaree Inthong ◽  
Thitichai Jarudecha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Genetic Characterization ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
The 16S Rrna Gene

Background and Aim: Hemoplasmas are defined as small, epicellular parasitic bacteria that can infect the red blood cells of several mammalian species. Diseases caused by these bacteria range from asymptomatic infections to acute hemolytic anemia. However, data on hemoplasmas in non-human primates in Thailand remain to be limited. Therefore, this study aims to determine the occurrence and genetic diversity of hemoplasmas among long-tailed macaques in Thailand. Materials and Methods: Blood samples were collected from 339 long-tailed macaques in three provinces of Thailand. DNA was then extracted from the blood samples and tested for hemoplasma using broad-range nested polymerase chain reaction (PCR) based on the 16S rRNA gene. PCR-positive samples were sequenced, and phylogenetic analysis for species identification was conducted. Results: In total, 38 (11.2%) out of the 339 samples were found to be positive for hemoplasmas, based on the broad-range nested PCR assay of the 16S rRNA gene. The 16S rRNA sequences of Mycoplasma spp. were highly similar (98-99% identity) to "Candidatus Mycoplasma haemomacaque." Furthermore, phylogenetic analysis using maximum likelihood demonstrated that the sequences were located in the same cluster of "Ca. M. haemomacaque." Conclusion: The detection of hemoplasmas among long-tailed macaques in Thailand is reported. Genetic characterization confirmed that these hemoplasmas are closely related to "Ca. M. haemomacaque." These results indicate that long-tailed macaques in several locations in Thailand may be infected and serve as reservoirs for this parasite.

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