scholarly journals Phenolics and carotenoids contents and radical scavenging capacity of some selected solanaceous medicinal plants

2018 ◽  
Vol 16 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Rumana Islam ◽  
Md Nesar Uddin ◽  
Md Ashrafuzzaman ◽  
Md Injamum Ul Hoque
Keyword(s):  
Medicinal Plants ◽  
Radical Scavenging ◽  
Solanum Nigrum ◽  
Fresh Weight ◽  
Solanum Torvum ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values ◽  
Phenolics Content ◽  
Fruit Phenolics

Plants being an important source of medicine play significant role in human health. The aim of the present study was to evaluate thetotal phenolics and carotenoids contents, and free radical scavenging capacity of leaves and fruits of selected five solanaceous medicinal plants, namely Solanum melongena (brinjal), Solanum torvum (tit begun), Solanum virginianum (kantikari), Solanum sisymbrifolium (sada kantikari) and Solanum nigrum (futi begun). Carotenoids content in the leaves and fruits of solanaceous plants varied significantly among the species. Leaf phenolics content ranged between 147.40 (S. melongena) and 585.15 (S. virginianum) mg gallic acid equivalent (GAE)/100 g fresh weight, while fruit phenolics content varied from 50.52 (S. nigrum) to 105.02 (S. virginianum) mg GAE/100 g fresh weight. IC50 values for scavenging 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) radical ranged between 31.52 (S. nigrum) and 33.55 (S. melongena) mg mL−1 in leaf, while in fruit it ranged between 27.90 (S. virginianum) and 33.11 (S. melongena) mg mL−1. The highest carotenoids content (0.370 mg g−1 fresh weight) was measured from Solanum nigrum leaf. S. virginianum leaf contained about 4−fold high phenolics content than that in S. melongena. S. nigrum leaf had about 15−fold high carotenoids content (0.370 mg g−1 fresh weight). compared to S. torvum and S. virginianum fruits (0.024 mg g−1FW in each). Because of the highest fruit phenolics and carotenoids content along with the lowest IC50 values for scavenging DPPH, S. virginianum fruit can be considered as superior for its health beneficial biochemical constituentsJ. Bangladesh Agril. Univ. 16(1): 56-61, April 2018

scholarly journals Quantification of Total Phenolics Content and Their Antioxidant Scavenging Capacity in Selected Herbs Extract

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Normah Haron ◽  
Noraslinda Muhammad Bunnori ◽  
Noor Hasniza Md Zin ◽  
Widya Abdul Wahab ◽  
Khairul Bariyyah Abdul Halim
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Strong Correlations ◽  
Dpph Assay ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phenolics Content ◽  
Gallic Acid Equivalent

Introduction: G. procumbens, H. sabdariffa and C. nutans contain potential compoundsthat lead to the development of drugs and supplements. The quantification of phenoliccompound was conducted by analyzing the compounds at 3 different fractions (free,insoluble bound and soluble bound). Thus, the objectives of this research were todetermine TPC and antioxidant of the plants in three different extracts. Methods: Threereplicates of the medicinal plants were extracted with 80% methanol and hydrolysis withHCl and NaOH. The TPC was determined by using Folin-Ciocalteau reagent whileantioxidant radical scavenging capacities were analyzed by using the DPPH assay. Results:Results showed that the TPC of the G. procumbens and C. nutans extracts of the insolublebound extract had the highest amount of phenolic compounds (15.19 ± 0.25 mg of GAE/gand 6.09+ 0.45 mg gallic acid equivalent GAE/g DW, respectively) as compared to theother fractions (p<0.05). In contrast, H. sabdariffa had the highest TPC value inboundsoluble phenolic compound (7.63 + 0.28 mg GAE/g DW) than the other fractions (p<0.05).The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts showedthat highest IC50 values in the insoluble bound extract > free > soluble bound extract,whereas the bound soluble of H. sabdariffa showed highest IC50 value > free >boundinsoluble phenolic extracts. Conclusions: Strong correlations were found between TPCwith antioxidant radical scavenging capacity. This finding proved that these three plantscould be a good source of natural antioxidant for food, cosmetic and pharmaceuticalindustry.

scholarly journals A simple new method for aged seed utilization based on melatonin-mediated germination and antioxidant nutrient production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Aged Seed ◽  
Phenolics Content ◽  
Dpph Radical Scavenging ◽  
Aged Seeds

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

scholarly journals Leaf phenolics and pigments along with their antioxidative potential in some medicinal plants

2018 ◽  
Vol 2 (2) ◽  
pp. 86-92
Author(s):  
Rumana Islam ◽  
Md Injamum Ul Hoque ◽  
Md Ashrafuzzaman ◽  
Md Nesar Uddin
Keyword(s):  
Medicinal Plants ◽  
Chlorophyll A ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Chlorophyll B ◽  
Fresh Weight ◽  
Adhatoda Vasica ◽  
Paederia Foetida ◽  
Phenolics Content ◽  
Boerhavia Diffusa

Medicinal plants play significant role in human health. Besides this all of the Unani, Herbal, Kobiraji, and Homeopath medicine are produced from medicinal plant. The aim of the present study was to evaluate the phytochemical constituents as total phenolics content, free radical scavenging capacity, chlorophyll a, chlorophyll b, total chlorophyll (a+b) and carotenoids. Leaves of seven important non-solanaceous medicinal plants namely Boerhavia diffusa (punarnava), Adhatoda vasica (basok), Paederia foetida (gandhabhaduli), Rauwolfia serpentina (sarpogandha), Catharanthus roseus (pink nayantara), Catharanthus alba (white nayantara) and Andrographis paniculata (kalomegh) were extracted for phenolics, chlorophyll a and b as well as carotenoids in this experiment. Total phenolics content in seven medicinal plants in this experiment ranged from 142.44 (C. roseus) to 416.25 (A. vasica) mg GAE/100 g leaf fresh weight. The IC50 value for scavenging 2, 2- diphenyl-1- picrylhydrazyl (DPPH) free radicals ranged from 32.12 (C. roseus) to 38.40 (Boerhavia diffusa) mg mL-1 leaf extract in seven different medicinal plants. Pigments content such as chlorophyll a, chlorophyll b, chlorophyll (a+b) and carotenoids were found highest in Adhatoda vasica leaf as 2.347, 0.900, 3.283, and 0.521 mg g-1 fresh weight, respectively, among all the seven species. We may get highly valuable medicine from Adhatoda vasica (basok) leaf for its superior biochemical constituents, which will help our next generation to treat chronic diseases. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 86-92

scholarly journals USE OF A FREE RADICAL SCAVENGING METHOD ON EXTRACTS OBTAINED BY MOLECULAR DISTILLATION FROM OREGANO ESSENTIAL OIL

2014 ◽  
Vol 44 (1) ◽  
pp. 25-30
Author(s):  
A. V. BORGARELLO ◽  
G. N. MEZZA ◽  
A. T. SOLTERMANN ◽  
M. C. PRAMPARO
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Molecular Distillation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Oregano Essential Oil ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values

There is evidence that oregano essential oil has a significant antioxidant effect on the process of lipid oxidation. In order to obtain fractions enriched in antioxidant properties, oregano essential oil samples were processed by molecular distillation. Molecular distillation experiments were done in two groups of tests, using an evaporating temperature between 22 and 30 ºC. Pressure was varied between 0.7 and 53 mbar. Antioxidant activity, specifically radical scavenging capacity, was analyzed in the essential oil and the distillates and residues obtained by a free radical scavenging method, using DPPH* (2,2-diphenyl-1picrylhydrazyl). The results obtained allowed to conclude that the free radical scavenging capacity was increased in the residue fractions obtained by molecular distillation. These fractions were concentrated in thymol. The IC50 values of the residues were similar to the synthetic antioxidant BHT, suggesting that fractions concentrated in oxygen derivatives obtained by molecular distillation could be used in the food industry to retard lipid degradation.

scholarly journals Antioxidant Activity of Different Forms of Green Tea: Loose Leaf, Bagged and Matcha

2018 ◽  
Vol 6 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Sumaya Farooq ◽  
Amit Sehgal
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Significant Difference ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Green tea is commercially available in three forms: loose leaf, bagged and powdered. The objective of this study was to compare the radical scavenging capacity of different forms of green tea like loose leaf (3), bagged (2) and powdered matcha (2) of various brands. The green tea forms were prepared at 95-100°C for 5 min., to mimic conditions usually used for tea preparations at home. The comparison of combined IC50 values of different green tea forms (loose leaf, bagged and matcha) showed no significant difference in their radical scavenging activity except bagged tea that exhibited slightly more DPPH radical scavenging potential as compared to matcha. Individually, the Bud white loose leaf demonstrated highest antioxidant activity followed by Laplant bag, Lipton bag, Laplant loose, Gourmet matcha, Wow matcha and Lipton loose. These findings revealed that on the basis of form, it may not be possible to generalize which form of tea whether loose leaf, bagged or matcha, is more effective in scavenging free radicals.

scholarly journals IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRACTIONATED EXTRACTS AND ISOLATION OF BIOACTIVE COMPONENTS FROM BREYNIA VITIS-IDAEA (BURM. F.) C. E. C. FISCHER LEAVES

2018 ◽  
Vol 54 (2C) ◽  
pp. 354
Author(s):  
Nguyễn Thị Lan Hương
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Bioactive Components ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

This study investigated the antioxidant and antibacterial activities of ethyl acetate, nbutanoland ethanol extracts of Breynia vitis-idaea (Burm.f.) C. E. C. Fischer leaves using invitro assays;and isolated bioactive compounds from the fractioned extract which showed the bestproperties by column chromatography. All extracts showed significant radical scavengingactivities and exhibited antibacterial activities against Enterococcus faecalis, Staphylococcusaureus and methicillin-resistant Staphylococcus aureus (MRSA). Ethyl acetate extracts showedthe highest free radical scavenging capacity with the IC50 values of 99.55 and 94.66 μg/ml (inDPPH and ABTS assays, respectively) and exhibited MIC values of 1.5, 1 and 1 mg/ml againstthe three bacteria, respectively. In addition, from ethyl acetate extracts, one pure compound hasbeen obtained and identified as 6-O-benzoylarbutin.

Sign in / Sign up

Export Citation Format

Share Document