scholarly journals Quantification of Total Phenolics Content and Their Antioxidant Scavenging Capacity in Selected Herbs Extract

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Normah Haron ◽  
Noraslinda Muhammad Bunnori ◽  
Noor Hasniza Md Zin ◽  
Widya Abdul Wahab ◽  
Khairul Bariyyah Abdul Halim
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Strong Correlations ◽  
Dpph Assay ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phenolics Content ◽  
Gallic Acid Equivalent

Introduction: G. procumbens, H. sabdariffa and C. nutans contain potential compoundsthat lead to the development of drugs and supplements. The quantification of phenoliccompound was conducted by analyzing the compounds at 3 different fractions (free,insoluble bound and soluble bound). Thus, the objectives of this research were todetermine TPC and antioxidant of the plants in three different extracts. Methods: Threereplicates of the medicinal plants were extracted with 80% methanol and hydrolysis withHCl and NaOH. The TPC was determined by using Folin-Ciocalteau reagent whileantioxidant radical scavenging capacities were analyzed by using the DPPH assay. Results:Results showed that the TPC of the G. procumbens and C. nutans extracts of the insolublebound extract had the highest amount of phenolic compounds (15.19 ± 0.25 mg of GAE/gand 6.09+ 0.45 mg gallic acid equivalent GAE/g DW, respectively) as compared to theother fractions (p<0.05). In contrast, H. sabdariffa had the highest TPC value inboundsoluble phenolic compound (7.63 + 0.28 mg GAE/g DW) than the other fractions (p<0.05).The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts showedthat highest IC50 values in the insoluble bound extract > free > soluble bound extract,whereas the bound soluble of H. sabdariffa showed highest IC50 value > free >boundinsoluble phenolic extracts. Conclusions: Strong correlations were found between TPCwith antioxidant radical scavenging capacity. This finding proved that these three plantscould be a good source of natural antioxidant for food, cosmetic and pharmaceuticalindustry.

scholarly journals A simple new method for aged seed utilization based on melatonin-mediated germination and antioxidant nutrient production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Aged Seed ◽  
Phenolics Content ◽  
Dpph Radical Scavenging ◽  
Aged Seeds

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.

scholarly journals Phenolics and carotenoids contents and radical scavenging capacity of some selected solanaceous medicinal plants

2018 ◽  
Vol 16 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Rumana Islam ◽  
Md Nesar Uddin ◽  
Md Ashrafuzzaman ◽  
Md Injamum Ul Hoque
Keyword(s):  
Medicinal Plants ◽  
Radical Scavenging ◽  
Solanum Nigrum ◽  
Fresh Weight ◽  
Solanum Torvum ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values ◽  
Phenolics Content ◽  
Fruit Phenolics

Plants being an important source of medicine play significant role in human health. The aim of the present study was to evaluate thetotal phenolics and carotenoids contents, and free radical scavenging capacity of leaves and fruits of selected five solanaceous medicinal plants, namely Solanum melongena (brinjal), Solanum torvum (tit begun), Solanum virginianum (kantikari), Solanum sisymbrifolium (sada kantikari) and Solanum nigrum (futi begun). Carotenoids content in the leaves and fruits of solanaceous plants varied significantly among the species. Leaf phenolics content ranged between 147.40 (S. melongena) and 585.15 (S. virginianum) mg gallic acid equivalent (GAE)/100 g fresh weight, while fruit phenolics content varied from 50.52 (S. nigrum) to 105.02 (S. virginianum) mg GAE/100 g fresh weight. IC50 values for scavenging 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) radical ranged between 31.52 (S. nigrum) and 33.55 (S. melongena) mg mL−1 in leaf, while in fruit it ranged between 27.90 (S. virginianum) and 33.11 (S. melongena) mg mL−1. The highest carotenoids content (0.370 mg g−1 fresh weight) was measured from Solanum nigrum leaf. S. virginianum leaf contained about 4−fold high phenolics content than that in S. melongena. S. nigrum leaf had about 15−fold high carotenoids content (0.370 mg g−1 fresh weight). compared to S. torvum and S. virginianum fruits (0.024 mg g−1FW in each). Because of the highest fruit phenolics and carotenoids content along with the lowest IC50 values for scavenging DPPH, S. virginianum fruit can be considered as superior for its health beneficial biochemical constituentsJ. Bangladesh Agril. Univ. 16(1): 56-61, April 2018

scholarly journals Antibacterial and Anticancer Activity of Ethnomedicinal Plants Used in the Jongilanga Community, Mpumalanga

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Lall N ◽  
De Canha MN ◽  
Reid A ◽  
Oosthuizen CB ◽  
Langhansova L ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Radical Scavenging ◽  
Docking Studies ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Value ◽  
Cox 2 ◽  
Antibacterial And Anticancer Activity

Seventy-four ethanolic extracts were prepared from traditionally used medicinal plants in the Jongilanga community in Mpumalanga South Africa. The aim was to determine the biological activity of the selected plants against cancer, mycobacteria species and acne. From the results, it was evident that Mundulea sericea was able to inhibit the proliferation of human melanoma cells (A375) with a fifty percent inhibitory concentration (IC50) ranging between 50 and 100 µg/ml as well as the ability to inhibit Mycobacterium tuberculosis, Mycobacterium smegmatis and Propionibacterium acnes with minimum inhibitory concentrations (MIC) of 125, 31.25 and 7.9 µg/ml respectively. This further led to the investigation of the antioxidant and anti-inflammatory activity as well as the influence of the extract on mycothiol disulphide reductase (Mtr) and glutathione reductase enzymes (Gtr) as potential targets against the above-mentioned diseases. M. sericea inhibited the COX-2 enzyme, responsible for inflammation, with an IC50 value of 10.70 ± 1.14 µg/ml, furthermore compounds previously isolated from M. sericea showed potential inhibition of COX-2 in molecular docking studies. Low radical scavenging capacity against the DPPH free radical with an IC50 value of 60.52 ± 2.40 µg/ml was obtained, however, M. sericea showed a higher affinity towards Mtr as compared to Gtr, which makes it an ideal plant for use as an antimycobacterial agent.

scholarly journals Volatile Variability and Antioxidant Activity of Rosmarinus officinalis Essential Oil as Affected by Elevation Gradient and Vegetal Associations

2019 ◽  
Vol 31 (6) ◽  
pp. 1279-1288 ◽  
Author(s):  
M. SABBAHI ◽  
A. EL-HASSOUNI ◽  
A. TAHANI ◽  
A. EL-BACHIRI
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Elevation Gradient ◽  
Radical Scavenging ◽  
Rosmarinus Officinalis ◽  
Gas Chromatography Mass Spectrometry ◽  
Dpph Assay ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Essential Oil Yield

The essential oil yield, volatile profile and antioxidant activity, of 37 samples collected from the Rosmarinus officinalis shrubs growing wild in the different ecosystems of the provinces of Taourirt and Jerada (Oriental region in the east of Morocco), were studied in order to determine the effect of the habitat and how the vegetal associations are influencing the volatile variability of their essential oil. The essential oil compositions were determined by gas chromatography-mass spectrometry (GC-MS). The yield of the essential oil extraction ranged from 1.09 to 2.81 %. The major components found were 1.8-cineol (37.71-65.02 %), camphor (6.09-27.49 %), α-pinene (3.08-9.98 %) and β-pinene (2.83-11.7 %). The interpretation of these results revealed that the variability is attributed to varieties and genetic background rather than the ecosystems. The antioxidant activity was determined by 1,1-diphenyl-1-picrylhydrazyl (DPPH) assay which revealed a strong radical scavenging capacity for all the samples with some variability which can be explained by the variability of the content of monoterpenes oxygenated.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

scholarly journals ANTIOXIDANT ACTIVITIES OF HYPERICUM FURSEI L.

2014 ◽  
Vol 44 (1) ◽  
pp. 19-23
Author(s):  
B. S. ALIPOUR ◽  
M. A. EBRAHIMZADEH ◽  
B. ESLAMI ◽  
Z. RAHMANI
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Chelating Ability ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

In this study, antioxidant activities of H. fursei were investigated. Different model were used for assay. IC50 for DPPH radical-scavenging activity was 7.5±1.2 μg ml-1. The IC50 value for BHA was 92.9±4.5 µg ml-1. The extract showed very good reducing power that was comparable with vitamin C (p> 0.05) but showed weak nitric oxide scavenging activity and Fe2+ chelating ability. Extract showed good activity in scavenging of H2O2. Inhibition was 99.1% at 800 μg ml-1. The IC50 values for extract and BHA were 333.1±14.7 and 52.0±4.5 μg ml-1, respectively. Total phenol compounds, by the Folin Ciocalteu method, was 274±9.6 mg gallic acid equivalent/g of extract and the total flavonoid content, by AlCl3 method was 23.9 ± 1.4 mg quercetin equivalent/g of extract. Antioxidant activity may be attributed to the presence of phenols and flavonoids in the extract.

scholarly journals Investigating the Antioxidant Characteristics of Saccharina Latissima and Its Ability to Reduce Inflammation in RAW 264.7 Cells (P06-091-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Abigail Wiegand ◽  
Gretchen Grebe ◽  
Carrie Byron ◽  
Angela Myracle
Keyword(s):  
Gallic Acid ◽  
Radical Scavenging ◽  
Saccharina Latissima ◽  
Total Phenolic ◽  
Raw 264.7 ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
The University ◽  
Gallic Acid Equivalent

Abstract Objectives The goal of this study is to understand the anti-inflammatory characteristics of Saccharina latissima (sugar kelp). Sugar kelp is a brown macroalga that grows readily off the coast of Maine. Other seaweeds are known to contain bioactive chemicals that reduce inflammation—a contributing factor to metabolic syndrome and type 2 diabetes. It is hypothesized that injuring farmed sugar kelp by trimming the kelp may increase the beneficial bioactives and promote anti-inflammatory effects. The specific aims are (1) to produce extracts of Maine-grown sugar kelp through accelerated solvent extraction (ASE), (2) to measure the samples’ antioxidant capacity, and (3) to investigate the samples’ anti-inflammatory effect in vitro. Methods Sugar kelp samples were freeze-dried and extracts were produced using ASE. Total phenolic content was measured using the Folin-Ciocalteau assay, and radical scavenging capacity was measured with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The sugar kelp extracts were tested for cytotoxicity in RAW 264.7 macrophages using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to induce inflammation in the cells, and the attenuation effect of extract doses ranging from 12.5–50 µg total phenolics (TP)/mL was observed by measuring nitric oxide (NO) secretion with the Griess assay. Results Preliminary data shows that sugar kelp contains polyphenols (8.4–16.0 mg gallic acid equivalent/g dried biomass) and radical scavenging capacity (IC50 = 61.8–79.5 µg/mL). The data did not demonstrate the trimming the kelp has a beneficial effect to increase phenolic compounds. The polyphenolic contents of the trimmed samples were lower than the untrimmed (9.5 vs. 13.1 mg gallic acid equivalent/g dried biomass). The radical scavenging capacity decreased when the samples were trimmed (14.7%). The greatest decrease in NO production was at a concentration of 50 μg TP/mL for a trimmed sample (up to 78.5%), which supports the hypothesis. Conclusions This data suggests that sugar kelp may be a good source of antioxidants and trimmed samples may contain additional bioactives to help decrease inflammation. Further investigation is needed to confirm the hypothesis. Funding Sources The University of Maine The University of New England

scholarly journals USE OF A FREE RADICAL SCAVENGING METHOD ON EXTRACTS OBTAINED BY MOLECULAR DISTILLATION FROM OREGANO ESSENTIAL OIL

2014 ◽  
Vol 44 (1) ◽  
pp. 25-30
Author(s):  
A. V. BORGARELLO ◽  
G. N. MEZZA ◽  
A. T. SOLTERMANN ◽  
M. C. PRAMPARO
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Molecular Distillation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Oregano Essential Oil ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values

There is evidence that oregano essential oil has a significant antioxidant effect on the process of lipid oxidation. In order to obtain fractions enriched in antioxidant properties, oregano essential oil samples were processed by molecular distillation. Molecular distillation experiments were done in two groups of tests, using an evaporating temperature between 22 and 30 ºC. Pressure was varied between 0.7 and 53 mbar. Antioxidant activity, specifically radical scavenging capacity, was analyzed in the essential oil and the distillates and residues obtained by a free radical scavenging method, using DPPH* (2,2-diphenyl-1picrylhydrazyl). The results obtained allowed to conclude that the free radical scavenging capacity was increased in the residue fractions obtained by molecular distillation. These fractions were concentrated in thymol. The IC50 values of the residues were similar to the synthetic antioxidant BHT, suggesting that fractions concentrated in oxygen derivatives obtained by molecular distillation could be used in the food industry to retard lipid degradation.

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