scholarly journals Assessment of haematological, serum biochemical and histopathological effects of subchronic dietary fumonisin b1 in rats

1970 ◽  
Vol 18 ◽  
pp. 74-83
Author(s):  
FA Gbore ◽  
A Ologundudu ◽  
AO Arowosegbe ◽  
TM Ologun ◽  
FA Jolade ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Live Weight ◽  
Toxicity Study ◽  
Feed Consumption ◽  
Short Term ◽  
Female Wistar Rats ◽  
Serum Biochemical ◽  
Histopathological Effects

Context: Fumonisin B1 (FB1), a mycotoxin produced by Fusarium verticillioides and other Fusarium species that grow on maize worldwide, has been documented to cause various physiological responses in animals. Consumption of lesser amounts of fumonisins at levels below those that cause overt toxicity may exert haematological, serum biochemical and/or histopathological effects in animals.   Objective: The effects of dietary FB1 on haematology, serum biochemistry and histopathology were assessed in female Wistar rats in a short-term toxicity study.   Materials and Methods: Thirty-nine mature female Wistar rats (Rattus norvegicus) weighing between 167.5 – 170.5 g were used in the study. The rats were assigned to diets containing 0.2, 10.0 and 20.0 mg FB1/kg constituting diets 1, 2 and 3, respectively. After 14 days of feeding, blood samples were obtained from four rats per treatment. The rats were sacrificed by cervical dislocation, eviscerated for organ collections and subsequently processed for histology.   Results: Significant differences in feed consumption and body weight gains were not observed. The final live weight of the rats, however, seemed to decline with an increase in dietary FB1 levels. Significant (P<0.05) alterations were observed in the haematological and serum biochemical parameters with increasing levels of dietary FB1. Diets containing different FB1 concentrations, the decreased values of PCV, Hb, erythrocyte and monocyte counts could be attributed to the FB1 effects on the blood-forming tissues in animals placed on diets 2 and 3 as compared to those fed diet 1. Also, histopathological changes were observed in the livers, kidneys, spleens and hearts of rats fed diets 2 and 3.   Conclusion: This study revealed that the No-observable adverse effect level (NOAEL) of dietary FB1 above which may cause significant physiological changes without overt toxicity for short-term toxicity study in female Wistar rats is <0.74 mg/kg bw per day.   Keywords: Blood; Rat; Mycotoxin; Fusarium verticillioides; Fumonisin B1; Histopathology. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8779 JBS 2010; 18(0): 74-83

scholarly journals Safety Evaluation of Multiple Strains ofLactobacillus plantarumandPediococcus pentosaceusin Wistar Rats Based on the Ames Test and a 28-Day Feeding Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Cheng-Chih Tsai ◽  
Sew-Fen Leu ◽  
Quan-Rong Huang ◽  
Lan-Chun Chou ◽  
Chun-Chih Huang
Keyword(s):  
Wistar Rats ◽  
Clinical Chemistry ◽  
Toxicity Study ◽  
Bacterial Strains ◽  
Oral Toxicity ◽  
Short Term ◽  
Mutagenic Potential ◽  
Multiple Strains

Three lactic acid bacterial strains,Lactobacillus plantarum, HK006, and HK109, andPediococcus pentosaceusPP31 exhibit probiotic potential as antiallergy agents, both in vitro and in vivo. However, the safety of these new strains requires evaluation when isolated from infant faeces or pickled cabbage. Multiple strains (HK006, HK109, and PP31) were subject to a bacterial reverse mutation assay and a short-term oral toxicity study. The powder product exhibited mutagenic potential inSalmonellaTyphimurium strains TA98 and TA1535 (with or without metabolic activation). In the short-term oral toxicity study, rats received a normal dosage of 390 mg/kg/d (approximately9×109 CFU/kg/d) or a high dosage of 1950 mg/kg/d (approximately4.5×1010 CFU/kg/d) for 28 d. No adverse effects were observed regarding the general condition, behaviour, growth, feed and water consumption, haematology, clinical chemistry indices, organ weights, or histopathologic analysis of the rats. These studies have demonstrated that the consumption of multiple bacterial strains is not associated with any signs of mutagenicity ofS.Typhimurium or toxicity in Wistar rats, even after consuming large quantities of bacteria.

scholarly journals Acute and Subacute Toxicity Studies of the Ethyl Acetate Soluble Proanthocyanidins of the Immature Inflorescence of Cocos nucifera L. in Female Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
C. P. Ekanayake ◽  
M. G. Thammitiyagodage ◽  
S. Padumadasa ◽  
B. Seneviratne ◽  
C. Padumadasa ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Wistar Rats ◽  
Cocos Nucifera ◽  
Toxicity Study ◽  
Immature Inflorescence ◽  
Subacute Toxicity ◽  
Toxicity Studies ◽  
Female Wistar Rats

Ayurvedic and traditional medical practitioners of Sri Lanka use the decoction of the immature inflorescence of Cocos nucifera L. (IC) variety aurantiaca for the treatment of menorrhagia. The progestogenic effect of the ethyl acetate soluble proanthocyanidins (EASPA) of the IC in female rats at a dose of 3.5 mg/kg body weight has been reported. Acute and subacute toxicity studies of EASPA of the IC carried out using female Wistar rats according to Organization for Economic Co-operation and Development (OECD) guidelines 423 and 407, respectively, are reported herein. In the acute toxicity study, a single dose of EASPA (2000 mg/kg body weight) was orally administered to rats, which were monitored for 14 days. In the subacute toxicity study, rats were orally administered with EASPA daily for 28 days at doses of 1.75, 3.5, 7, and 14 mg/kg body weight. No rat in either the acute or subacute toxicity study exhibited mortality or clinical signs of toxicity. Further, these rats did not show any significant change in their mean body weight, food, and water intake, haematological and biochemical parameters as well as in the results of their histopathological examinations compared to those of control group rats. According to results of the acute toxicity, the LD50 of EASPA is estimated to be greater than 2000 mg/kg body weight. Considering the results of the subacute toxicity study, the oral administration of EASPA daily for 28 days was well tolerated up to the dose, 14 mg/kg by rats. These results will be useful in the development of a novel therapeutic agent from EASPA of the IC for the treatment of menorrhagia, which incapacitates a considerable proportion of women worldwide.

OESTROGEN AND THE CONTROL OF GONADOTROPHIN SECRETION IN THE IMMATURE RAT

1974 ◽  
Vol 63 (2) ◽  
pp. 285-298 ◽  
Author(s):  
F. DÖCKE ◽  
G. DÖRNER
Keyword(s):  
Wistar Rats ◽  
Short Term ◽  
Oestrogen Treatment ◽  
Immature Rat ◽  
Inhibiting Effect ◽  
Gonadotrophin Secretion ◽  
Oestradiol Benzoate ◽  
Female Wistar Rats ◽  
Implantation Method

SUMMARY Experiments were performed in female Wistar rats on the mode of action of oestrogen in affecting gonadotrophin secretion during infancy. Using an improved implantation method, former findings on a hypophysial site of oestrogen action in the Hohlweg effect were confirmed. The sensitivity to the ovulation-inducing effect of oestradiol benzoate (OB) increased as the rats approached the age of natural puberty. The first spontaneous ovulation could be suppressed by intrahypophysial, but not by intrahypothalamic, progesterone implants. A single s.c. injection or intracranial administration of OB at 25 or 26 days of age, although leading to premature vaginal opening (VO) and, in some of the animals, to one ovulation, did not induce true precocious puberty. To accelerate the onset of puberty, 0·05 μg OB/100 g body wt had to be injected daily from 5 days of age to VO, or from day 5 to day 10 and, additionally, from day 26 to VO. After long-term oestrogen treatment, the gonadotrophin-inhibiting effect of OB implanted into the middle hypothalamus from 26 to 34 days of age was significantly reduced in comparison with untreated control rats. A final experiment demonstrated that the first ovarian cycle was not prolonged after neonatal ovariectomy and implantation of ovaries at 24, 28 or 32 days of age. The results indicate that similar neurohormonal mechanisms are operational at the first pubertal and at later cyclic ovulations. They also indicate that the maturation of the gonadotrophin-controlling mechanisms continues during infancy in the absence of ovarian steroids. It can be accelerated in Wistar rats by long-term, but not by short-term prepubertal oestrogen treatment.

scholarly journals Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Suparmi Suparmi ◽  
Minidian Fasitasari ◽  
Martanto Martosupono ◽  
Jubhar Christian Mangimbulude
Keyword(s):  
Oxidative Stress ◽  
Sodium Nitrate ◽  
Wistar Rats ◽  
Histological Evaluation ◽  
Female Wistar Rats ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Serum Biochemical ◽  
Hb Concentration ◽  
Rbc Count

Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll fromSauropus androgynus(L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll fromS. androgynusand Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2exposure resulted in significant reductions (p<0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p>0.05) to normal values. Chlorophyll fromS. androgynusand Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

scholarly journals Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALB/c Mice Model

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Heshu Sulaiman Rahman ◽  
Abdullah Rasedee ◽  
Hemn Hassan Othman ◽  
Max Stanley Chartrand ◽  
Farideh Namvar ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Toxicity ◽  
Biochemical Parameters ◽  
Toxicity Study ◽  
Feed Consumption ◽  
Nanostructured Lipid Carrier ◽  
Serum Biochemical Parameters ◽  
Acute Toxicity Study ◽  
Serum Biochemical ◽  
Brain Tissues

Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemia effectin vitrowas evaluated for its toxicological effects by observing changes in the liver, kidney, spleen, lung, heart, and brain tissues, serum biochemical parameters, total haemogram, and bone marrow stem cells. The acute toxicity study for ZER-NLC was conducted by orally treating BALB/c mice with a single dose with either water, olive oil, ZER, NLC, or ZER-NLC for 14 days. The animals were observed for clinical and behavioral abnormalities, toxicological symptoms, feed consumption, and gross appearance. The liver, kidney, heart, lung, spleen, and brain tissues were assessed histologically. Total haemogram was counted by hemocytometry and microhematocrit reader. Bone marrow examination in terms of cellular morphology was done by Wright staining with bone marrow smear. Furthermore, serum biochemical parameters were determined spectrophotometrically. Grossly all treated mice, their investigated tissues, serum biochemical parameters, total haemogram, and bone marrow were normal. At oral doses of 100 and 200 mg/kg ZER-NLC there was no sign of toxicity or mortality in BALB/c mice. This study suggests that the 50% lethal dose (LD50) of ZER-NLC is higher than 200 mg/kg, thus, safe by oral administration.

Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats

2021 ◽  
Author(s):  
Milica R Prvulovic ◽  
Desanka J Milanovic ◽  
Predrag Z Vujovic ◽  
Milena S Jovic ◽  
Selma D Kanazir ◽  
...  
Keyword(s):  
Calorie Restriction ◽  
Early Onset ◽  
Wistar Rats ◽  
Late Onset ◽  
Daily Intake ◽  
Short Term ◽  
Cognitive Frailty ◽  
Y Maze ◽  
Female Wistar Rats ◽  
Potential Benefits

Abstract The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical–cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal’s behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.

Dominant lethal effects in male and female wistar rats after short-term alpha-asarone treatment

1996 ◽  
Vol 88 ◽  
pp. 67 ◽  
Author(s):  
Germán Chamorro ◽  
María Salazar ◽  
Silvia Salazar ◽  
Joaquín Tamariz
Keyword(s):  
Wistar Rats ◽  
Short Term ◽  
Lethal Effects ◽  
Male And Female ◽  
Dominant Lethal ◽  
Female Wistar Rats

scholarly journals Short-term effects of feeding rats with glucose syrup fractions and dextrose

1973 ◽  
Vol 29 (1) ◽  
pp. 87-93 ◽  
Author(s):  
G. G. Birch ◽  
I. J. Etheridge ◽  
L. F. Green
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Wistar Rats ◽  
Young Female ◽  
Low Molecular Weight ◽  
Dietary Carbohydrate ◽  
Short Term ◽  
Dextrose Equivalent ◽  
Glucose Syrup ◽  
Female Wistar Rats

1. Young female Wistar rats were fed for 18 d on diets containing dextrose, 43 dextrose equivalent (DE) glucose syrup, fractions of 43 DE glucose syrup of high and low molecular weight or sucrose.2. All rats gained weight and showed no gross external abnormalities or significant dental caries scores.3. The concentrations of cholesterol, triglycerides, free fatty acids and glucose were determined in samples of liver and serum. Generally, these values tended to increase with increasing molecular weight of dietary carbohydrate in the order dextrose, glucose syrup fraction of low molecular weight, and glucose syrup fraction of high molecular weight.4. Rats given the high-molecular weight glucose syrup fraction were found to have caecums twice the normal size.

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