scholarly journals Antagonistic Potentials of Selected Soil Fungi Against Three Pathogenic Fungi of Tagetes Erecta L. and T. Patula L

2020 ◽  
Vol 29 (2) ◽  
pp. 147-153
Author(s):  
Mahfuza Aktar ◽  
Shamim Shamsi

Three antagonistic fungi were isolated from the field soil of blight infected Tagetes spp. by serial dilution method. The fungi were identified as Aspergillus flavus Link, A. niger van Tiegh. and Trichoderma viride Pers. Antagonistic potentiality of aforesaid fungi were evaluated against the pathogenic fungi of Tageteserecta L. and T. patula L. following "dual culture colony interaction" and volatile and nonvolatile metabolites. The pathogenic fungi were A. alternata, Aspergillus fumigatus and Curvularia lunata. In dual culture colony interaction, out of three soil fungi, T. viride showed the highest growth inhibition on A. alternata (71.03%), A. fumigatus (38.49%) and C. lunata (60.71%). The maximum inhibition of radial growth of A. alternata (74.55%) was observed with the culture filtrates of T. viride owing to volatile metabolites. The maximum inhibition of radial growth of A. fumigatus (37.43%) was observed with the culture filtrates of A. flavus owing to volatile metabolites. The complete inhibition of radial growth of C. lunata was observed with the culture filtrates of A. niger owing to volatile metabolites. The complete inhibition of radial growth of A. alternata was observed with nonvolatile metabolites of A. nigera and T. viride at all concentrations. The complete inhibition of radial growth of A. fumigatus was also observed with nonvolatile metabolites of A. niger at all concentrations. Aspergillus niger and T. viride may be exploited commercially as a biocontrol agent against blight pathogens of T. erecta and T. patula. Dhaka Univ. J. Biol. Sci. 29(2): 147-153, 2020 (July)

2019 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Rezuana Binte Helal ◽  
Shamim Shamsi

Four antagonistic fungi were isolated from the papaya field soil by serial dilution and were identified as Aspergillus flavus Link., A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potentiality against the three postharvest pathogenic fungi of Carica papaya L. following "dual culture colony interaction” and volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride showed the highest growth inhibition on C. gloeosporioides (84.28%), F. nivale (95.23%) and Fusarium sp. (87.15%). On the other hand A. niger showed the highest growth inhibition on C. gloeosporioides (77.39%), F. nivale (98.63%) and Fusarium sp. (35.05%). The maximum inhibition of radial growth of C. gloeosporioides (77.64%), F. nivale (58.76%) and Fusarium sp. (79.37%). were observed in case of T. viride owing to the volatile metabolites. Whereas the maximum inhibition of radial growth of C. gloeosporioides (58.23%), F. nivale (37.43%) and Fusarium sp. (82.31%) were observed in case of A. niger owing to the volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (90.90%), F. nivale (89.13%) and Fusarium sp. (76.84%) were observed in case of T. viride owing to the effect of non-volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (92.42%), F. nivale (73.01%) and Fusarium sp. (68.67%) were observed in case of A. niger owing to the effect of non-volatile metabolites. Trichoderma viride and A. niger may be exploited commercially as a biocontrol agent against anthracnose and fruit rot pathogens of papaya. Dhaka Univ. J. Biol. Sci. 28(1): 1-7, 2019 (January)


2019 ◽  
Vol 43 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Md Delwar Hosen ◽  
Shamim Shamsi

Four soil fungi were isolated from the soil by serial dilution and were identified as Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potential against seed borne fungus Fusarium merismoides isolated from sesame. In dual culture colony interaction Trichoderma viride showed the highest (45.88%) growth inhibiting effect on F. merismoides followed by A. niger (40.00%), A. flavus (36.37) and A. fumigatus (30.77%). Volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (67.69%) and non-volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (75.00%). Journal of Bangladesh Academy of Sciences, Vol. 43, No. 1, 17-23, 2019


2021 ◽  
Vol 46 (2) ◽  
pp. 143-153
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
Abul Bashar

The antagonistic potentials of four soil filamentous fungi viz., Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. against six pathogens isolated from 14 varieties of cotton (Gossypium hirsutum L.) were evaluated by "dual culture colony interaction”, volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride was found be the most effective to control the growth of the cotton pathogens. T. viride showed the highest growth inhibition against Curvularia lunata, Fusarium moniliforme, Mucor sp. and Rhizoctonia solani. On the other hand A. niger showed the highest growth inhibition against Fusarium nivale and A. fumigatus showed the maximum growth inhibition against C. gloeosporioides. The highest inhibition of radial growth of C. lunata, F. nivale and F. moniliforme was observed might be due to the secretion of volatile metabolites of T. viride whereas, the maximum inhibition of radial growth of C. gloeosporioides was observed because of the volatile metabolites of A. flavus. Mucor sp. and R. solani were inhibited due to the volatile metabolites of A. fumigatus. The greatest radial growth inhibition of C. lunata and F. moniliforme were noticed in case of T. viride owing to the effect of non-volatile metabolites. On the other hand, the radial growth of C. gloeosporioides and F. nivale were inhibited highest amount for the effect of non-volatile metabolites of A. niger, whereas highest growth inhibition of Mucor sp. and R. solani was observed due to the non-volatile effect of A. fumigatus. The present investigation suggests that the isolates of Aspergillus and Trichoderma may be further exploited as potential biocontrol agents against the fungal pathogens of cotton in field trial. Asiat. Soc. Bangladesh, Sci. 46(2): 143-153, December 2020


2015 ◽  
Vol 24 (2) ◽  
pp. 137-145
Author(s):  
Romana Akter ◽  
KS Hossain ◽  
MA Bashar

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)


2015 ◽  
Vol 43 (2) ◽  
pp. 213-217 ◽  
Author(s):  
MT Aktar ◽  
KS Hossain ◽  
MA Bashar

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested in opposition to six pathogenic fungi viz., Colletotrichum sp., Curvularia lunata, Fusarium moniliforme, F. oxysporum, F. semitectum and Phomopsis sp. isolated from different leaf spots and fruit rots of brinjal. Out of seven soil fungi, Trichoderma harzianum was found most effective to control the growth of all the test fungi in the study of colony interactions and effects of volatile and non-volatile metabolites. This fungus may be exploited commercially to biocontrol the diseases. DOI: http://dx.doi.org/10.3329/bjb.v43i2.21675 Bangladesh J. Bot. 43(2): 213-217, 2014 (September)


2015 ◽  
Vol 49 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Mousumi Bhadra ◽  
Abul Khair ◽  
Md Anawar Hossain ◽  
Md Maniruzzaman Sikder

Experiments were carried out to find out the bio-efficacy of four Trichoderma species, viz. Trichoderma harzianum, T.koningii, T.viride (green strain), T.viride (yellow strain) against canker pathogen Lasiodiplodia theobromae. Bioassay of antagonist against test pathogens conducted by dual culture techniques at different temperatures; volatile, non volatile and naturally untreated metabolites of isolates were examined. T. koningii and T.viride (yellow strain) exhibited maximum inhibition in controlling the pathogens. Fungicides, viz. Bavistin and Dithane M-45 used where Bavistin found little effective but Dithane M-45 showed no effects on pathogen. Trichoderma viride showed better performance to control Lasiodiplodia theobromae than commercial fungicides used during present investigation. DOI: http://dx.doi.org/10.3329/bjsir.v49i2.22008 Bangladesh J. Sci. Ind. Res. 49(2), 125-130, 2014


2019 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Nahla Tharwat Elazab

In recent year’s endophytic fungi has become a major concern on their host plants by enhancing their growth, increasing their fitness, strengthening their tolerances to abiotic and biotic stresses, and promoting their accumulation of secondary metabolites. Kingdom of Saudi Arabia has a wide range of flora which may be a rich source of endophytic fungi so that, the present study involves diversity and bioactivity of the endophytic fungal community in Al-Qassim region from 15 wild plants 162 isolates were obtained and identified. Among them, the most common isolates were Aspergillus niger, Aspergillus terreus, Aspergillus ochraceous and Trichoderma viride, these four endophytic isolates were examined for its antagonistic effect against six phytopathogenic fungi using two different assays, Dual-culture and Culture filtrate. Trichoderma viride recorded the most significant growth inhibition of almost pathogenic fungi followed by the three endophytic Aspergillus spp. In addition, these four endophytic fungi were screened for the production of some extracellular enzymes such as protease, cellulose, amylase, pectinase and xylanase. Our results show the ability of these isolates to produce these extracellular enzymes so this indicated the possible role of endophytic fungi as a biocontrol agent of plant disease.


2019 ◽  
Vol 28 (2) ◽  
pp. 219-226
Author(s):  
Zuhra Yasmin ◽  
Shamim Shamsi

Four antagonistic fungi Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. were isolated from field soil of Rauwolfia serpentina by serial dilution method and selected to evaluate their antagonistic potentiality against Colletotrichum gloeosporiodes (Penz.) Sacc, the causal agent of anthracnose of Rauwolfia serpentina (L.) Benth. ex Kurz following dual culture colony interaction, volatile and non-volatile metabolites. In dual culture method Trichoderma viride showed maximum (84.28%) inhibition of test fungi followed by Aspergillus niger (77.39%), A. fumigatus (43.71%) and A. flavus (29.32%). Volatile metabolites of T. viride showed higher (77.64%) inhibition of test fungi followed by A. flavus (75.58%), A. fumigatus (60.88%) and A. niger (58.23%). Non-volatile metabolites of antagonistic fungi showed that A. flavus showed (94.42%) inhibition of test fungus followed by T. viride (90.90%), A. niger (86.13%) and A. fumigatus (73.73%). Aspergillus flavus, A. niger and T. viride may be exploited commercially as a biocontrol agent against anthracnose disease of R. serpentina. Dhaka Univ. J. Biol. Sci. 28(2): 219-226, 2019 (July)


2010 ◽  
Vol 10 ◽  
pp. 760-765 ◽  
Author(s):  
S. C. Sati ◽  
P. Arya

The antagonistic activity of five aquatic hyphomycetes, viz.,Heliscus lugdunensis,Tetrachaetum elegans,Tetracladium breve,T. marchalianum, andT. nainitalense, against seven plant pathogenic fungi was studied using a dual culture technique. Inhibitory activity of tested aquatic hyphomycetes was determined by measuring the radial growth of plant pathogenic fungi on dual culture plates.Tetrachaetum elegansshowed antagonistic activity againstColletotrichum falcatum,Fusarium oxysporum,Pyricularia oryzae,Sclerotium sclerotiorum, andTilletia indicaHeliscus lugdunensisshowed antagonism against only two plant pathogenic fungi,Rhizoctonia solaniandColletotrichum falcatum.Tetracladium breve,T. marchalianum, andT. nainitalenseshowed no response towards tested plant pathogenic fungi.


2018 ◽  
Vol 6 (2) ◽  
pp. 59-66
Author(s):  
Raihan I Raju

A number of seed borne fungi such as- Fusarium moniliforme, Curvularia lunata, Alternaria solani, Helminthosporium solani, Aspergillus flavus, A. niger and Penicillium spp. were identified from the selected BARI-8, Nilima, Patharkuchi, Roma and Ratan varieties of tomato seeds. The highest (2.53%) mean incidence of the fungi F. moniliforme were recorded in tomato variety Roma, BARI-8, Nilima and Ratan while the lowest (0.13%) mean incidence of the fungi Penicillium was found in Ratan. The maximum percent of germination (64%) was recorded in both BARI-8 and Patharkuchi varieties, while the minimum (7%) germination in Nilima. The highest seedling vigor index (632.96) was encountered in BARI-8 and the lowest in Nilima. Trichoderma viride, T. harzianum and T. koningii were used as bio-control agents against major seed borne fungi. T. viride showed the significantly effective mycelial growth inhibition against F. moniliforme and C. lunata, while T. harzianum and T. koningii showed poor performance against both tested fungi. In case of botanical extracts, Lawsonia inermis exhibited the maximum (82.15% and 86.24%) inhibition of mycelium growth of F. moniliforme and C. lunata, respectively. Chemical fungicides, Bavistin (0.5 gm/l) inhibited 65% mycelial growth against F. moniliforme, while higher concentration of chemical fungicide gave 81% inhibition of C. lunata. The results suggested that, L. inermis phytoextract can possibly be exploited for the management of seed-borne pathogenic fungi of tomato in an environmental friendly way.Jahangirnagar University J. Biol. Sci. 6(2): 59-66, 2017 (December)


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