scholarly journals Comparative phytochemical analysis of the aerial parts of Empetrum nigrum L. samples, collected in various regions of the Russian Federation

2021 ◽  
Vol 10 (4) ◽  
pp. 138-145
Author(s):  
A. O. Ponkratova ◽  
A. K. Whaley ◽  
E. V. Zhokhova ◽  
E. A. Bezverkhniaia ◽  
V. G. Luzhanin
Keyword(s):  
Chemical Composition ◽  
Secondary Metabolites ◽  
Russian Federation ◽  
Kamchatka Peninsula ◽  
Leningrad Region ◽  
Empetrum Nigrum ◽  
Drug Candidates ◽  
Aerial Parts ◽  
Hplc Profiles ◽  
Reasonable Choice

Introduction. A number of studies have shown that various genetic and environmental factors can affect the biosynthesis and accumulation of secondary metabolites. In particular, it is known that the local geoclimate, seasonal changes, external conditions such as light, temperature, moisture and soil fertility can affect the chemical composition and, as a result, the therapeutic properties of plants used in the pharmaceutical and food industries. Empetrum nigrum L. is a rich source of various pharmacologically active secondary metabolites – chalcones, dihydrochalcones, bibenzyls, 9,10-dihydrophenanthrenes, flavonoids, and proanthocyanidins. In the scientific literature, there is no data on the variation in the chemical composition of E. nigrum depending on the growing area. The obtained data are necessary for a reasonable choice of the collecting location for the plant, with the aim of its further chemical and pharmacological research for the development of promising drug candidates.Aim. To carry out a comparative analysis of secondary metabolites composition in the aerial parts of Empetrum nigrum growing in different regions of the Russian Federation.Materials and methods. Samples collected in three different areas were used to compare HPLC profiles: sample 1 was collected next to SPCPU nursery garden of medicinal plants (Leningrad region, Vsevolozhsky district, Priozerskoe highway, 38 km) in July 2020, sample 2 was collected on the Kola Peninsula, around the Khibiny mountains in July 2020, sample 3 was collected on the Kamchatka Peninsula, next to Khalaktyrsky beach (Pacific Ocean coast) in July 2020. Extracts were analyzed by analytical high performance liquid chromatography (HPLC) using a Prominence LC-20 device (Shimadzu, Japan) equipped with a diode array detector.Results and discussion. As a result of the research, for the first time, a significant variation in the qualitative chemical composition in the aerial parts of Empetrum nigrum growing in different regions of Russian Federation was established. Sample 3, collected on the Kamchatka Peninsula, in comparison with samples 1 and 2, contain the greatest variety of polar secondary metabolites belonging to the classes of flavonoids, tannins, and phenol carboxylic acids, while in the shoots collected in the Leningrad region, the major metabolites were weakly polar compounds belonging to the classes of chalcones, dihydrochalcones, bibenzyls, and 9,10-dihydrophenanthrenes, and in sample 2, collected in the Khibiny mountains, the lowest qualitative content of secondary metabolites was found. This variation may be caused by various environmental factors (biotic and abiotic).Conclusion. For the first time, the comparison of HPLC profiles of aerial part samples of E. nigrum, collected in different regions of the Russia Federation has been carried out. As a result, significant variations in the secondary metabolites composition of the studied samples were established, depending on the regions and growing conditions of the plants. The data obtained can be used for a reasonable choice of the collection location for the plant, with the aim of its further chemical and pharmacological research for the development of promising drug candidates.

scholarly journals Using high performance thin layer chromatography for the detection of pharmacologically active secondary metabolites in Empetrum nigrum L.

2021 ◽  
Vol 10 (4) ◽  
pp. 129-137
Author(s):  
A. O. Ponkratova ◽  
A. K. Whaley ◽  
V. G. Luzhanin ◽  
E. V. Zhokhova
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Thin Layer ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Empetrum Nigrum ◽  
Aerial Parts ◽  
Pharmacologically Active ◽  
Layer Chromatography ◽  
Derivatives Of

Introduction. The article presents the results of the detection of pharmacologically active secondary metabolites in black crowberry Empetrum nigrum L. using the method of high performance thin layer chromatography (HPTLC).Aim. To show the efficiency of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites in promising medicinal plant species.Materials and methods. HPTLC analysis was carried out on a CAMAG device (Switzerland), using MERCK HPTLC silica gel 60 F154, 20 × 10 cm plates. For the evaporation of the samples, a Heidolph vacuum rotary evaporator (Germany) was used. The aerial parts (shoots) of Empetrum nigrum were harvested next to St. Petersburg State Chemical and Pharmaceutical University (SPCPU) nursery garden of medicinal plants (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in August 2019.Results and discussion. In the course of the research, four fractions from the aerial parts of Empetrum nigrum were obtained: hexane, dichloromethane, butanol, and water. Then, these fractions were investigated by HPTLC in two solvent systems – n-butanol : acetic acid : water (BAW) (4 : 1 : 2) and hexane : dichloromethane : methanol (HDM) (1 : 2 : 0.5). After scanning densitometric analysis of the plates eluted in the HDM system, it was revealed, that the hexane and dichloromethane fractions have a similar composition and contain the greatest amount of compounds, compared to the butanol and water fractions, and in the BAW system, it was found, that the butanol fraction contains the greatest variety of metabolites. As a result of UV spectroscopy, it was found, that the main groups of compounds contained in the hexane and dichloromethane fractions are derivatives of chalcones, dihydrochalcones, bibenzyls and 9,10-dihydrophenanthrenes. While in the butanol fraction, the main groups of secondary metabolites were derivatives of flavonoids and tanninsConclusion. The data obtained allow us to note the efficiency, speed and simplicity of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites of promising medicinal plant species.

Secondary metabolites from the aerial parts of Stachys tetragona, a Greek endemic species

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Afouxenidi ◽  
T Milošević-Ifantis ◽  
H Skaltsa
Keyword(s):  
Secondary Metabolites ◽  
Endemic Species ◽  
Aerial Parts

New data on aphyllophoraceous fungi (Basidiomycota) of the protected areas of the Leningrad Region. X. State Nature Reserve «Kurgalsky»

2018 ◽  
Vol 52 (1) ◽  
pp. 101-121 ◽  
Author(s):  
V. M. Kotkova
Keyword(s):  
Protected Areas ◽  
Russian Federation ◽  
Protected Area ◽  
Nature Reserve ◽  
Leningrad Region ◽  
State Nature Reserve ◽  
X State ◽  
First Time ◽  
Aphyllophoroid Fungi ◽  
State Nature

The paper provides the data on aphyllophoroid fungi of the State Nature Reserve «Kurgalsky» situated in the Kingisepp District of the Leningrad Region. The list includes 285 species annotated by data on their habitats, substrates and frequency. In total 25 species protected in the Leningrad Region and 3 species protected in Russian Federation were found in the protected area. Chaetodermella luna, Phlebia subochracea and Trechispora stevensonii are published for the first time for the Leningrad Region. The specimens of selected species are kept in the Mycological Herbarium of the Komarov Botanical Institute RAS (LE).

A criminal profiling of persons convicted for narcotic drugs and psychotropic substances illicit trafficking

2020 ◽  
Vol 2020 (3) ◽  
pp. 118-122
Author(s):  
Elizaveta Pavlik ◽  
Elena Ketenchieva
Keyword(s):  
Russian Federation ◽  
Leningrad Region ◽  
Public Interests ◽  
Drug Offenders ◽  
Psychotropic Substances ◽  
Judicial Practice ◽  
The Russian Federation ◽  
Criminal Profile ◽  
Illicit Trafficking ◽  
Narcotic Drugs

This article provides a classification and characteristics of persons convicted for crimes in the field of narcotic drugs and psychotropic substances illicit trafficking. The article deals with the statistics in this sphere over the past five years. Based on the collected empirical evidence the authors analyzed the qualitative indicators of convicted drug offenders. The examination of statistical data from investigative and judicial practice on crimes in the field of narcotic drugs and psychotropic substances illicit trafficking made it possible to sketch a criminal profile of a person sentenced to imprisonment: a citizen of the Russian Federation, a male between 18 to 39 years, having a secondary general or vocational education. Moreover, the personality of a convicted person is characterized by his internal criteria deformation devaluing the norms and rules that express and consolidate public interests. The authors also conducted a comparative analysis of offenders convicted both in the Russian Federation and in such federal subjects as Saint-Petersburg and Leningrad region. The choice of St. Petersburg as one of the objects of this research is justified by the fact that it has been among the leaders of the regions with the largest number of recorded drug-related crimes for several years.

Chemical Composition, Antibacterial, Insecticidal and Anti-Oxidant Activities of Three Acantholimon Species (A. atropatanum, A. gilliatii and A. tragacanthium)

2020 ◽  
Vol 10 (3) ◽  
pp. 272-278
Author(s):  
Ardalan Pasdaran ◽  
Satyajit D. Sarker ◽  
Lutfun Nahar ◽  
Azadeh Hamedi
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Main Compound ◽  
Aerial Parts ◽  
Anti Oxidant ◽  
Gas Chromatography Mass Spectroscopy

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.

A new bibenzyl and 9,10-dihydrophenanthrene derivative from aerial parts of crowberry (Empetrum nigrum L.)

2021 ◽  
Vol 42 ◽  
pp. 15-17
Author(s):  
Anastasiia O. Ponkratova ◽  
Andrei K. Whaley ◽  
Olga A. Balabas ◽  
Sergey N. Smirnov ◽  
Peter Proksch ◽  
...  
Keyword(s):  
Empetrum Nigrum ◽  
Aerial Parts

scholarly journals Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Janusz Malarz ◽  
Klaudia Michalska ◽  
Anna Stojakowska
Keyword(s):  
Chemical Composition ◽  
Plant Material ◽  
Methanolic Extract ◽  
Sesquiterpene Lactones ◽  
Scavenging Activity ◽  
Nmr Analysis ◽  
Caffeic Acid Derivatives ◽  
Aerial Parts ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The objective of the present study was to characterize chemical composition of hitherto unexamined aerial parts of Lactuca sativa var. angustana cv. Grüner Stern. In contrast to leafy and head varieties of the lettuces, asparagus lettuce grown in Europe is much less studied. Fractionation of a methanolic extract from leaves of L. sativa cv. Grüner Stern, supported with HPLC/DAD and 1H NMR analysis, led to the isolation and/or identification of numerous terpenoid and phenolic compounds, including five apocarotenoids—(-)-loliolide, (+)-dehydrovomifoliol, blumenol A, (6S,9S)-vomifoliol, and corchoionoside C; three sesquiterpene lactones; two lignans—((+)-syringaresinol and its 4-O-β-glucoside); five caffeic acid derivatives; and three flavonoids. Some of the compounds, to the best of our knowledge, have never been isolated from L. sativa before. Moreover, monolignols, phenolic acids and a tryptophan-derived alkaloid were found in the analyzed plant material. Stems, leaves and shoot tips of the asparagus lettuce were examined to assess their phenolics and sesquiterpene lactone content as well as DPPH scavenging activity. Another stem lettuce—L. sativa var. angustana cv. Karola, two cultivars of leafy lettuces and one species of wild lettuce—L. serriola, were also examined as a reference material using HPLC/DAD. The results have been discussed regarding our previous studies and the literature data available.

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