scholarly journals Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed

2021 ◽  
Vol 10 (2) ◽  
pp. e19510212373
Author(s):  
Diego Figueiredo da Costa ◽  
Davidianne de Andrade Morais ◽  
Aline Ferreira da Silva ◽  
Ismael Lira Borges ◽  
Jefferson da Silva Ferreira ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Post Infection ◽  
Statistical Difference ◽  
Agglutination Test ◽  
Vaginal Fluid ◽  
Human Beings ◽  
Molecular Analyses ◽  
Experimental Infections ◽  
Histopathologic Diagnosis ◽  
Significant Difference

Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona.  A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation.

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

scholarly journals Immune Responses and Protection against Experimental Brucella suis Biovar 1 Challenge in Nonvaccinated or B. abortus Strain RB51-Vaccinated Cattle

2010 ◽  
Vol 17 (12) ◽  
pp. 1891-1895 ◽  
Author(s):  
S. C. Olsen ◽  
S. G. Hennager
Keyword(s):  
Immune Responses ◽  
Experimental Infection ◽  
Brucella Abortus ◽  
Agglutination Test ◽  
Saline Control ◽  
Infected Cattle ◽  
Infection Rates ◽  
Treatment Groups ◽  
Brucella Suis ◽  
Fluorescence Polarization Assay

ABSTRACT Twenty Hereford heifers approximately 9 months of age were vaccinated with saline (control) or 2 × 1010 CFU of the Brucella abortus strain RB51 (RB51) vaccine. Immunologic responses after inoculation demonstrated significantly greater (P < 0.05) antibody and proliferative responses to RB51 antigens in cattle vaccinated with RB51 than in the controls. Pregnant cattle received a conjunctival challenge at approximately 6 months of gestation with 107 CFU of B. suis bv. 1 strains isolated from naturally infected cattle. The fluorescence polarization assay and the buffered acid plate agglutination test had the highest sensitivities in detecting B. suis-infected cattle between 2 and 12 weeks after experimental infection. Serologic responses and lymphocyte proliferative responses to B. suis antigens did not differ between control and RB51 vaccinees after experimental infection. No abortions occurred in cattle in either treatment group after challenge, although there appeared to be an increased incidence of retained placenta after parturition in both the control and the RB51 vaccination treatment groups. Our data suggest that the mammary gland is a preferred site for B. suis localization in cattle. Vaccination with RB51 did not reduce B. suis infection rates in maternal or fetal tissues. In conclusion, although B. suis is unlikely to cause abortions and fetal losses in cattle, our data suggest that RB51 vaccination will not protect cattle against B. suis infection after exposure.

Efficacy of ivermectin against Parastrongylus malaysiensis infection in rats

1992 ◽  
Vol 66 (4) ◽  
pp. 293-296
Author(s):  
S. Ambu ◽  
J. W. Mak ◽  
C. S. Ng
Keyword(s):  
Post Infection ◽  
Larval Stages ◽  
Experimental Infections ◽  
Significant Pathology

ABSTRACTThe efficacy of ivermectin on experimental infections of P. malaysiensis in rats was determined. Ivermectin was 99·4% and 97·9% effective at a dosage of 400 mcg and 800 mcg respectively at seven days post-infection. The same two dosages of ivermectin when given at 14 days post infection had an efficacy of 100%. However, as an adulticide it had only 40·7% efficacy. Ivermectin may therefore be useful for the treatment of parastrongyliasis due to the larval stages of the worm which can cause significant pathology in man and animals.

The effect of Hyoscine Butylbromide on the rate of vaginal delivery in cases of secondary arrest of cervical dilatation: A rondomized controlled clinical trial

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohamed Ahmed Elkady ◽  
Dina Yahia Mansour ◽  
Alyaa Mostafa Elshamy
Keyword(s):  
Vaginal Delivery ◽  
Statistical Difference ◽  
Saline Group ◽  
Cervical Dilatation ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Hyoscine Butylbromide ◽  
Labor Time ◽  
Significant Statistical Difference ◽  
Significant Difference

Abstract Background For decades, health providers have worked to manage labor actively and safely, with the goal of shortening the duration of painful labor. Reduction of cesarean sections and maternal complication is also an important aspect of labor management. Antispasmodic are commonly administrating during labor in both developing and developed countries, although there is a paucity of scientific reports validating this. Hyoscine butylbromide is an effective antispasmodic drug. Therefore, We aim to assess efficacy of Hyoscine butylbromide on rate of vaginal delivery in cases of 2ry arrest of cervical dilatation. Objective To assess whether the intravenous injection of hyoscine butylbromide is effective in progress of labor in which cases with 2ry arrest of cervical dilatation. Patients and Methods The study included 100 pregnant women (PG, P1, P2, P3) conducted at Ain Shams University Maternity Hospital, the inclusion Criteria were: term uncomplicated singleton pregnancy in a spontaneous active labor, with cephalic presentation. The patient must be free from any medical disorder associated with pregnancy and free from any obstetric complication. Cases were subdivided into 2 groups: Study group: 50 patients received 40mg iv Hyoscine Butylbromide (as Buscopan 20mg\mrl) when the cervix &gt;4cm dilated and was &gt;50% effaced. Control group: 50 patients received 2ml saline as placebo. Results Significant increase in vaginal delivery rate (72%) in Hyoscine group; compared to Saline group (52%); with significant statistical difference (p = 0.04). Highly significant decrease in 1st and 2nd stage labor time in Hyoscine group; compared to Saline group; with highly significant statistical difference (p &lt; 0.05 respectively). These results observed without significant difference as regards 3rd stage labor time and postpartum hemorrhage (p &gt; 0.05), APGAR score and NICU admission (p &gt; 0.05). Conclusion From the current study we can conclude that HBB 40 mg is safe and effective in increasing vaginal delivary rate in cases of 2ry arrest of 12 dilatation. It is effective in decreasing first and second stage of labor. It has no maternal or neonatal adverse effect. It is easy and available.

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals Fish Value Chain and Its Impact on Rural Households’ Income: Lessons Learned from Northern Ethiopia

2018 ◽  
Vol 10 (10) ◽  
pp. 3759 ◽  
Author(s):  
Abebe Alemu ◽  
Hossein Azadi
Keyword(s):  
Sex Education ◽  
Value Chain ◽  
Rural Households ◽  
Lessons Learned ◽  
Northern Ethiopia ◽  
Productive Capacity ◽  
Human Beings ◽  
Significant Difference ◽  
Aquatic Food ◽  
The Government

These days, one of the global challenges is the growing demand for food. To be more specific, seafood bases play a key role in filling the nutritional requirements of human beings. In Africa (Ethiopia) the public expenses to improve productive capacity in aquatic food are increasing. Additionally, the expenses in dams and in fishers’ capacity building have increased households’ engagement in the fishery sector in Ethiopia. Cooperatives’ productive capacity has been strengthened by the government and other non-government organizations with the supply of fishing boats, refrigerators, fish nets and other office supplies. However, the effect of such public expenses in bringing changes in the households’ livelihood and welfare has never been assessed in this study area. This paper aims to investigate what motivates the households to fish and assess the effect of fisheries on the households’ livelihood and welfare. A structured survey consisting of 313 rural households was administered using trained enumerators in two kebeles located close to the Tekeze dam, Northern Ethiopia. The result indicates that socioeconomic characteristics, such as age (young), sex, education, and active family size were driving the households to fishing. Access to market and access to support are driving farmers to fisheries. There is a significant difference in fishing households’ income which is higher than non-fishing households. The results also indicate that there are lesser income inequalities among fishery households operating in cooperatives compared to private fishery households.

THE LIFE CYCLE OF TRICHINELLA SPIRALIS: I. THE INTESTINAL PHASE OF DEVELOPMENT

1967 ◽  
Vol 45 (6) ◽  
pp. 1255-1260 ◽  
Author(s):  
C. S. Shanta ◽  
E. Meerovitch
Keyword(s):  
Life Cycle ◽  
Post Infection ◽  
Sexual Maturity ◽  
Trichinella Spiralis ◽  
Seminal Vesicles ◽  
Intestinal Phase ◽  
Experimental Infections

In experimental infections in mice, Trichinella spiralis larvae in the intestines molted twice before reaching sexual maturity. In both sexes, the first molt occurred between 12 and 16 hours post infection; in males, the second molt occurred between 24 and 32 hours, and in females, between 22 and 30 hours. The females were inseminated after the 36th hour post infection, but some males had spermatozoa in the seminal vesicles before the completion of the second molt. Structures, believed to be amphids, were observed after 2 hours post infection; they increased in size up to the 6th hour, after which they regressed and finally disappeared. The function of these amphids is believed to be related to osmoregulation.

Experimental infection of the cockroachPeriplaneta americanawithToxocara canisand the establishment of patent infections in pups

2008 ◽  
Vol 82 (2) ◽  
pp. 97-100 ◽  
Author(s):  
N.K. Sasmal ◽  
T.K. Pahari ◽  
R. Laha
Keyword(s):  
Experimental Infection ◽  
Post Infection ◽  
Periplaneta Americana ◽  
Toxocara Canis ◽  
Tissue Migration ◽  
Eggs And Larvae

AbstractThe possible role of the cockroachPeriplaneta americanain the transmission ofToxocara caniseggs and larvae via faeces and tissue migration was studied. Cockroaches fed with 3 × 105and 5 × 105embryonated eggs were found to harbour viable eggs and larvae from days 1 to 5 post-infection (DPI). At necropsy on 5 DPI, eggs and larvae were also recovered from the rectal contents but not from the tissues of cockroaches. In addition patent infections were established in pups fed on infected faeces of cockroaches, with eggs first appearing in the faeces of pups at 38 DPI. Adult worms ofT. caniswere also recovered at necropsy. Therefore the importance of cockroaches as good mechanical disseminators of ascarid eggs, especiallyT. canis, is discussed.

Do-It-Yourself Microsuture from Human Hair for Basic Microsurgical Training

2018 ◽  
Vol 35 (05) ◽  
pp. 315-321
Author(s):  
Pobe Luangjarmekorn ◽  
Pravit Kitidumrongsook ◽  
Sittisak Honsawek
Keyword(s):  
Human Hair ◽  
Statistical Difference ◽  
Smooth Curve ◽  
P Value ◽  
Excellent Quality ◽  
Likert Score ◽  
Significant Difference ◽  
Do It Yourself ◽  
Knot Security

Background Microsuture is an essential material for basic microsurgical training. However, it is consumable, expensive, and sometimes unavailable in the microsurgical laboratory. To solve this problem, we developed a microsuture made from human hair and needle gauge. Methods Do-It-Yourself (DIY) microsuture is made from human hair and needle gauge 32G (BD Ultra-Fine Pen Needles 4 mm × 32G). Methods are explained step by step. This DIY microsuture (labeled as “test microsuture”) and nylon 8–0 (Ethilon suture 8–0, labeled as “standard microsuture”) were used for teaching orthopaedic residents to perform arterial anastomosis in chicken thighs. All residents practiced without knowing that “test microsuture” was made from the DIY method. After completing the training, quality of both microsutures was evaluated by questionnaire in topics of (1) thread quality (size, strength, elasticity, handing, knot perform, and knot security), (2) needle quality (size, curve, shape, sharpness, handling, and strength), (3) needle–thread interface (size, strength, and smoothness), and (4) overall quality of microsuture. Each category was evaluated by Likert score (5 = excellent, 4 = good, 3 = fair, 2 = poor, and 1 = very poor). Results The DIY microsuture was performed in three steps: (1) insert human hair into needle gauge by microforceps, (2) bend needle into smooth curve, and (3) disconnect needle and create needle–hair interface. The questionnaire was completed by 30 orthopaedic residents and showed that thread quality of DIY and standard microsuture had “good” and “good-to-excellent” quality (mean Likert score: 3.77–4.23 and 3.80–4.27, respectively, with no statistical difference). Thread–needle interface quality of DIY and standard microsuture also had “good” and “good-to-excellent” quality (Likert score: 3.73–4.20 and 4.07–4.33, respectively, with no statistical difference). Needle part of DIY microsuture had lower quality than standard suture (fair-to-good compared with good-to-excellence quality, score 3.30–3.67 vs. 4.20–4.27, respectively, with a statistically significant difference, p-value < 0.05). However, overall quality of DIY suture and standard microsuture had “good” and “good-to-excellent” (mean Likert score: 3.73 and 4.00, respectively, with no statistical difference). Conclusion The DIY microsuture from human hair and needle gauge could be an alternative for basic microsurgical training with lower cost, easy production, and more availability for use in practice with acceptable quality compared with that of standard microsuture.

scholarly journals Sero-prevalence of Leptospira spp. in Household and Stray Dogs by Microscopic Agglutination Test

2020 ◽  
Vol 1 (1) ◽  
pp. 3-6
Author(s):  
Ali Shabestari Asl ◽  
Mohammad Bafandehzendeh ◽  
Gholamreza Abdollahpoor
Keyword(s):  
Zoonotic Disease ◽  
Climatic Conditions ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Human Beings ◽  
Serum Samples ◽  
Stray Dogs ◽  
Growth And Survival ◽  
Leptospira Serovars ◽  
Micro Organisms

Background: Leptospirosis is a zoonotic disease of worldwide significance in human beings and many animals. This zoonotic disease causes infection in dogs and other pet animals and these animals can influence human’s health. Given the unknown prevalence of leptospirosis in dogs in our area, this study was performed to investigate the sero-epidemiology of leptospirosis and evaluate the seroprevalence of Leptospira serovars in both household and stray dogs by microscopic agglutination test (MAT). Methods: Serum samples of 90 dogs in two household and stray groups (45 dogs for each group) were collected. Specimens were examined by MAT (in accordance with the procedure recommended by WHO) against antigens of serovars Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona, Hardjo, Autumnalis, and Ballum. Results: In total, 6.7% of (n=6) dogs (1 stray and 5 household dogs) had a serum titer of 1:100 or more against Leptospira serovars. Dominant serovars were Canicola (8.9%), Grippotyphosa (2.2%), and Pomona (2.2%), respectively. The antibody titer against more than one serovar was observed only in one dog. The sero-prevalence was 11.1% in household dogs and 2.2% in stray dogs. Since 4 pet dogs had been vaccinated recently, it seems that the actual percentage of infection in household dogs must be just 2.2% and actual infection in dogs including stray dogs is approximately 4.4% in Tabriz. Conclusion: Fortunately, the rate of infection in Tabriz is low and this can be due to special geographical and climatic conditions, regular and annual vaccination of household dogs against this disease, and lack of suitable conditions for the growth and survival of such micro-organisms in the environment

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