scholarly journals Different Research Approaches in Unraveling the Venom Proteome of Naja ashei

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1282
Author(s):  
Konrad Kamil Hus ◽  
Łukasz Marczak ◽  
Vladimír Petrilla ◽  
Monika Petrillová ◽  
Jaroslav Legáth ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Protein Composition ◽  
Process Data ◽  
Qualitative And Quantitative ◽  
Dynamic Development ◽  
Proteomic Data ◽  
Ms Analysis ◽  
New Information ◽  
Proteomic Methods

The dynamic development of venomics in recent years has resulted in a significant increase in publicly available proteomic data. The information contained therein is often used for comparisons between different datasets and to draw biological conclusions therefrom. In this article, we aimed to show the possible differences that can arise, in the final results of the proteomic experiment, while using different research workflows. We applied two software solutions (PeptideShaker and MaxQuant) to process data from shotgun LC-MS/MS analysis of Naja ashei venom and collate it with the previous report concerning this species. We were able to provide new information regarding the protein composition of this venom but also present the qualitative and quantitative limitations of currently used proteomic methods. Moreover, we reported a rapid and straightforward technique for the separation of the fraction of proteins from the three-finger toxin family. Our results underline the necessary caution in the interpretation of data based on a comparative analysis of data derived from different studies.

scholarly journals TMT-based Proteomic Analysis and Protein Expression During Excystation of Cryptosporidium Anderson

2021 ◽  
Author(s):  
Dongfang Li ◽  
Zhaohui Cui ◽  
Luyang Wang ◽  
Kaihui Zhang ◽  
Letian Cao ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Vaccine Development ◽  
Protein Composition ◽  
Tandem Mass ◽  
Liquid Chromatograph ◽  
Control Of Gene Expression ◽  
Proteomic Data ◽  
Cryptosporidium Andersoni ◽  
New Information ◽  
Infected Adult

Abstract Background: Cryptosporidium andersoni (C. andersoni) initiates infection by the release of sporozoites through excystation. However, the proteins involved in excystation remain unknown. Researching the proteins that participate in the excystation of C. andersoni oocysts will fill the gap in our understanding of the excystation system of this parasitic pathogen. Methods: In this study, C. andersoni oocysts were collected and purified from the feces of naturally infected adult cows. Tandem mass tags (TMT) coupled with liquid chromatograph- tandem mass spectrometry (LC-MS/MS) proteomic analysis was used to investigate the proteomic expression profile of C. andersoni oocysts during excystation. Results: Our proteomic analysis identified a total of 1586 proteins, of which 17 were identified as differentially expressed proteins (DEPs), with 10 upregulated and 7 downregulated proteins. Each of those 17 proteins had multiple biological functions associated with control of gene expression at the level of transcription and biosynthetic and metabolic processes. Quantitative real-time PCR of eight selected genes validated the proteomic data.Conclusions: Our findings provide new information on the protein composition of C. andersoni oocysts as well as possible excystation factors. These data may help us to better understand the pathology of C. andersoni and thus may be useful in diagnosis, vaccine development, and immunotherapy for Cryptosporidium.

A comparative analysis of various parts of Ascaris suum with respect to their protein composition

1981 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
Won Young Choi ◽  
Young Kwan Jin ◽  
Ok Ran Lee ◽  
Woon Gyu Kim
Keyword(s):  
Comparative Analysis ◽  
Ascaris Suum ◽  
Protein Composition

Comparative analysis of essential oil obtained from fresh and dried leaves of medicinal sage

2020 ◽  
pp. 39-53
Author(s):  
Tatyana Shcheglova
Keyword(s):  
Comparative Analysis ◽  
Essential Oil ◽  
Raw Materials ◽  
Quantitative Difference ◽  
Botanical Garden ◽  
Component Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Qualitative And Quantitative ◽  
Salvia Officinalis L ◽  
Medical University

The article presents results of the study of a comparative analysis of essential oil obtained from fresh and dried leaves of medicinal sage (Salvia officinalis L.), growing in the Botanical Garden of the First Moscow State Medical University named after Sechenov. The studies were carried out using gas chromatography– mass spectrometry. Qualitative and quantitative difference in the component composition of the essential oil obtained from fresh and dried raw materials was determined as a result of the study.

Recommendations for deploying multidimensio

2017 ◽  
Vol 11 (1) ◽  
pp. 68-80
Author(s):  
E. E. Akimkina
Keyword(s):  
Comparative Analysis ◽  
Management System ◽  
Analytical Data ◽  
Enterprise Management ◽  
Multidimensional Data ◽  
Dimensional Approach ◽  
Process Data ◽  
Modeling Systems ◽  
Enterprise Management System ◽  
Practical Recommendations

A comparative analysis of different approaches to analytical data and shows that the most ample opportunities has a multi-dimensional approach, implemented with the help of OLAP technology. Presented multidimensional OLAP-cube model with the measurements for the analysis and processing of process data. Practical recommendations for the deployment of a multidimensional data modeling systems with regard to their integration into existing enterprise management system.

scholarly journals Molecular Species of the Alcohol Biomarker Phosphatidylethanol in Human Blood Measured by LC-MS

2009 ◽  
Vol 55 (7) ◽  
pp. 1395-1405 ◽  
Author(s):  
Anders Helander ◽  
Yufang Zheng
Keyword(s):  
Fatty Acid ◽  
Human Blood ◽  
Molecular Species ◽  
Quantitative Measurement ◽  
Selected Ion Monitoring ◽  
Blood Samples ◽  
Qualitative And Quantitative ◽  
Ms Analysis ◽  
Alcohol Biomarker ◽  
Product Ions

Abstract Background: The alcohol biomarker phosphatidylethanol (PEth) comprises a group of ethanol-derived phospholipids formed from phosphatidylcholine by phospholipase D. The PEth molecular species have a common phosphoethanol head group onto which 2 fatty acid moieties are attached. We developed an electrospray ionization (ESI) LC-MS method for qualitative and quantitative measurement of different PEth species in human blood. Methods: We subjected a total lipid extract of whole blood to HPLC gradient separation on a C4 column and performed LC-ESI-MS analysis using selected ion monitoring of deprotonated molecules for the PEth species and phosphatidylpropanol (internal standard). Identification of individual PEth species was based on ESI–tandem mass spectrometry (MS/MS) analysis of product ions. Results: The fatty acid moieties were the major product ions of PEth, based on comparison with PEth-16:0/16:0, 18:1/18:1, and 16:0/18:1 reference material. For LC-MS analysis of different PEth species in blood, we used a calibration curve covering 0.2–7.0 μmol/L PEth-16:0/18:1. The lower limit of quantitation of the method was <0.1 μmol/L, and intra- and interassay CVs were <9% and <11%. In blood samples collected from 38 alcohol patients, the total PEth concentration ranged between 0.1 and 21.7 μmol/L (mean 8.9). PEth-16:0/18:1 and 16:0/18:2 were the predominant molecular species, accounting for approximately 37% and 25%, respectively, of total PEth. PEth-16:0/20:4 and mixtures of 18:1/18:1 plus 18:0/18:2 (not separated using selected ion monitoring because of identical molecular masses) and 16:0/20:3 plus 18:1/18.2 made up approximately 13%, 12%, and 8%. Conclusions: This LC-MS method allows simultaneous qualitative and quantitative measurement of several PEth molecular species in whole blood samples.

Conditions for the Introduction of Regulation for Short-Term Rentals

2021 ◽  
Vol 8 (1) ◽  
pp. 159-170
Author(s):  
Vilim Brezina ◽  
◽  
Jan Polívka ◽  
Martin Stark ◽  
◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Housing Market ◽  
Housing Stock ◽  
Qualitative Comparative Analysis ◽  
Short Term ◽  
Market Conditions ◽  
Qualitative And Quantitative ◽  
Market Situation ◽  
Necessary And Sufficient ◽  
German Cities

Most cities in major agglomerations in Europe started to address the rise of short-term accommodation rentals by introducing regulation designed to protect the local housing stock. The momentum behind the widespread introduction of such regulations can be attributed to qualitative and quantitative factors. This article examines selected fields related to short-term rentals in order to uncover the (structural) triggers or conditions that are necessary and sufficient for municipalities to initiate the regulation of their housing market. The study is based on the systematic examination of the effects of those triggers and their combinations using qualitative comparative analysis (QCA). With this method, we explore the implementation or non-implementation of regulation on a sample of major German cities. The results suggest a universal set of conditions covering three central fields: housing market situation, accommodation market conditions and tourism accommodation demand.

scholarly journals THE VISUAL SYSTEM: A "CHICKEN AND EGG" PROBLEM SOLVED

2009 ◽  
Vol 05 (01) ◽  
pp. 115-121
Author(s):  
ANDREW R. PARKER ◽  
H. JOHN CAULFIELD
Keyword(s):  
Visual System ◽  
Neural Plasticity ◽  
Neural Circuit ◽  
Process Data ◽  
System A ◽  
New Information ◽  
The Brain

"What comes first: the chicken or the egg?" Eyes and vision were a great concern for Darwin. Recently, religious fundamentalists have started to attack evolution on the grounds that this is a chicken and egg problem. How could eyes improve without the brain module to use the new information that eye provides? But how could the brain evolve a neural circuit to process data not available to it until a new eye capability emerges? We argue that neural plasticity in the brain allows it to make use of essentially any useful information the eye can produce. And it does so easily within the animal's lifetime. Richard Gregory suggested something like this 40 years ago. Our work resolves a problem with his otherwise-insightful work.

Taxonomy and distribution of the Brazilian species of Thylamys (Didelphimorphia: Didelphidae) / La taxonomie et la distribution des espèces brésiliennes du genre Thylamys (Didelphimorphia: Didelphidae)

Mammalia ◽  
2006 ◽  
Vol 70 (1-2) ◽  
Author(s):  
Ana Paula Carmignotto ◽  
Talitha Monfort
Keyword(s):  
Natural History ◽  
Geographic Distribution ◽  
Taxonomic Status ◽  
Skull Morphology ◽  
Geographic Variations ◽  
Qualitative And Quantitative ◽  
Brazilian Species ◽  
New Information ◽  
Capture Method ◽  
Scientific Collections

AbstractThe recent increase in mammal inventories and the widespread use of pitfall traps as a major capture method in Brazil have increased the number of non-volant small mammal specimens in scientific collections, providing new information on natural history, geographical range, and taxonomic status of many marsupials and rodents. To date, however, little is known about the Brazilian species of the marsupial genus Thylamys : the number of species in the country is disputed, as are their names and geographic distribution. Thus, the aim of this study was to define the Brazilian species of the genus, delimiting their taxonomic status and distributional range. We provide qualitative and quantitative data on external and skull morphology for specimens housed in scientific collections. A study of the material available allowed us to recognize the occurrence of three species in Brazil: Thylamys karimii (Petter, 1968), T. macrurus (Olfers, 1818) and T. velutinus (Wagner, 1842). For each of these we provide redescriptions, a discussion of local and geographic variations, geographic distribution, taxonomy, age class differentiation, sexual dimorphism, and natural history.

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