scholarly journals ANTIFUNGAL POTENTIAL OF COMMERCIALLY AVAILABLE FUNGICIDES AGAINST ALTERNARIA BLIGHT OF BRASSICA

2018 ◽  
Vol 30 (1) ◽  
pp. 53
Author(s):  
Ahmed Subhani ◽  
Muhammad Atiq ◽  
Nasir A. Rajput ◽  
Muhammad R. Bashir ◽  
Muhammad Asif ◽  
...  

Brassica campestris is the leading oilseed crop of Pakistan. It fulfills 20% oil requirement of Pakistan. This crop is affected by various biotic and abiotic factors. Among all other yield limiting biotic constraints, Alternaria blight, caused by Alternaria brassicae playes imperative role. In the current research fifteen advanced lines/genotypes (Punjab Canola, Faisal Canola, Shirale, Dunkeld, Rainbow, AC-Excel, Legend, Oscar, Cyclone, Con II, Con III, Bulbul 98, DGL, B.S.A, Toria selection) were sown at Department of Plant Pathology with three replications under Completely Randomized Block Design (RCBD) by maintaining the plant to plant (P×P) distance of 45 cm and bed to bed (B×B) distance of 75 cm. Two genotypes i.e. Punjab canola (48.83%), Faisal canola (50.06%) exhibited moderately resistant response. Similarly, eight commercially available fungicides namely Rally 40% WP (Myclobutanil 40%), Chlorotoxin (Azoxystrobin 5.4%+Chlorothalonil 45%), Alliette 80% WP (Fosetyli-Aluminium 80%), Success 70% WP (Metalaxyl 8% + Chlorothalonil 64%), Swing 72% (Metalaxyl 8% +Mancozeb 64%), Cytrol 75% (Thiophanate-methyl 35% + Chlorothalonil 40%), Antracol (Propineb 70%) and Score 250 EC (Difenconazole 250EC) were used with two concentrations viz. 0.25% and 0.50% of each fungicide under filed conditions with three replication and Randomized Complete Block Design. It was observed that both concentrations (0.25% and 0.50%) of swing played statistically significant role to inhibit the disease incidence. Therefore, it is concluded that Punjab Canola and Faisal Canola are statistically reliable advanced lines as compared to all other thirteen advanced lines/ genotypes. Likewise, the concentrations 0.25% and 0.50% of swing have statistically more potential to retard the disease incidence as compared to all other commercially available fungicides.

2018 ◽  
Vol 24 (1) ◽  
pp. 43
Author(s):  
Syahrir Pakki ◽  
Mappaganggang Mappaganggang

<p>Two experiments to study responses of resistance of maize germplasm to downy mildew (Peronosclerospora philipinensis) have been conducted. Both experiments were arranged in randomized block design with two replication. The treatments in each experiment (2014 and 2016) were 70 maize accessions. The accessions were planted in planting distant of 75 cm × 20 cm, in 5 m rows, resulting in 20 plants per row. In every 10 rows, two check varieties were planted, i.e. one susceptible and one resistant variety. Ten days prior to planting, the soil was fertilized with mixed fertilizers consisted of urea, ZA, SP36, and KCl at a dose of 100, 100, 100, and 100 kg/ha, respectively. Fertilization II and III was given at 30 days after planting, and 45 days after planting with a dose of 100 kg urea/ha. As a source of inoculum downy mildew, around the repeated plot was planted with varieties Anoman variety (susceptible variety) that was inoculated with a suspension of downy mildew pathogen. Observation was conducted at 25, 35, and 55 days after planting, with the standard score of resistant to downy mildew, followed: 0–10% = resistant, 11–25% = moderately resistant, 26–50% = moderately susceptible, &gt;50% = susceptible. The result of the experiments showed that there were 5 accessions resistant to downy mildew (P. philipinensis), i.e. CML 440×MR4-9-30-3, 664, 60, 572, and 554 with the intensity of downy mildew infection ranged from 5 to 10%. Twelve other accessions were classified as moderately resistant, i.e. CML 440×MR4-9-98-2, 440×MR4-9-98-4 CML, CML 440×MR4-9-124-1, 66, 71, 319, 108, 73, 48, 105, 554, and 682 with intensity of infection ranged from 13 to 25%. At the same experiments, the average intensity of downy mildew infection on susceptible check varieties reached 100 percent.</p>


Revista CERES ◽  
2016 ◽  
Vol 63 (2) ◽  
pp. 214-222
Author(s):  
Francisco Thiago Coelho Bezerra ◽  
Alek Sandro Dutra ◽  
João Bosco Pitombeira

ABSTRACT The sunflower plant is an oilseed crop that has aroused a great interest in the Brazilian and international scenery especillay because of the possibility of using its oil for biodiesel production. The objective of this study was to evaluate productivity and yield components of Embrapa 122 sunflower according to the spatial arrangement. Treatments were arranged in 4 x 4 factorial arrangement, which are the four spacings between rows (0.30; 0.50; 0.70 and 0.90 m) and four sowing densities (30,000; 45,000; 60,000 and 75,000 plants ha-1). The experiment was carried out in a complete randomized block design with four replications. The experiments were conducted in the experimental area of the Plant Science Department in Fortaleza, State of Ceará-Brazil and on the Curu Vale Experimental Farm in Pentecoste, State of Ceará-Brazil. Productivity and the following production components were analyzed in the end of the crop cycle: harvested capitula, capitulum diameter, capitulum mass, achene mass per capitulum, mass of 100 achenes, achenes per capitulum, harvest index and oil content in the achenes. The experiments were analyzed jointly in relation to the cropping area and the data submitted to analysis of variance and quantitative factors tested by polynomial regression. The variables, spacing, density and cropping area did not interact with these variables and the spatial arrangement of the plants affected only the components. The cropping area influences the productive behavior of sunflower Embrapa 122. The spatial arrangement of the plants of sunflower of variety Embrapa 122 influences yield components but does not affect productivity.


2020 ◽  
Vol 13 (3) ◽  
pp. 81
Author(s):  
I G.A.A. INDRAYANI ◽  
SIWI SUMARTINI ◽  
B. HELIYANTO B. HELIYANTO

ABSTRAK<br />Amrasca biguttula (Ishida) adalah serangga hama pengisap daun<br />yang sangat potensial menurunkan produktivitas kapas. Pengendaliannya<br />secara kimiawi menimbulkan banyak masalah lingkungan, seperti<br />pencemaran dan peningkatan resistensi hama terhadap insektisida kimia<br />sintetis. Salah satu solusi dalam masalah tersebut adalah penggunaan<br />varietas tahan (resisten) yang juga merupakan bagian dari pengendalian<br />hama terpadu (PHT) pada kapas. Penelitian ketahanan beberapa aksesi<br />kapas terhadap A. biguttula (Ishida) dilakukan di Kebun Percobaan Balai<br />Penelitian Tanaman Tembakau dan Serat di Asembagus, Situbondo, mulai<br />Januari hingga Desember 2006. Tujuannya adalah untuk mengetahui<br />ketahanan beberapa aksesi kapas terhadap serangan hama pengisap daun,<br />A. biguttula. Sebagai perlakuan adalah 30 aksesi kapas yang ditanam<br />dalam plot berukuran 10 m x 3 m, dengan jarak tanam 100 cm x 25 cm,<br />satu tanaman per lobang. Setiap aksesi disusun dalam rancangan acak<br />kelompok dengan tiga kali ulangan. Parameter yang diamati adalah jumlah<br />nimfa A. biguttula per daun, jumlah bulu daun per cm 2 luas daun, dan<br />posisi bulu terhadap lamina (tegak/rebah), serta skor kerusakan tanaman.<br />Hasil penelitian menunjukkan bahwa setiap aksesi kapas berpotensi<br />terserang A. biguttula, meskipun tingkat populasi hama ini tidak<br />menunjukkan perbedaan nyata antar aksesi. Terjadi korelasi negatif (R 2 =<br />0,2425) antara jumlah bulu daun dan populasi nimfa A. biguttula dan<br />antara jumlah bulu daun dan skor kerusakan tanaman (R 2 = 0,2027).<br />Berdasarkan jumlah bulu daun, aksesi kapas yang termasuk kategori<br />sedikit berbulu dengan kriteria ketahanan sedikit tahan adalah: AC 134,<br />Stoneville 7, Fai Nai, SHR, CRDI-1, Kanesia 5, Kanesia 8, dan Kanesia 9.<br />Sedangkan aksesi lainnya termasuk kategori tidak berbulu dan peka<br />terhadap serangan A. biguttula.<br />Kata kunci : Kapas, Gossypium hirsutum, aksesi, hama, Amrasca<br />biguttula (Ishida), toleran, peka, kerusakan, Jawa Timur<br />ABSTRACT<br />Resistance of several cotton accessions to sucking insect<br />pest, Amrasca biguttula (Ishida)<br />Amrasca biguttula (Ishida) is a sucking insect pest which potentially<br />reduces cotton productivity. Its chemical control often cause environ-<br />mental problems mainly air pollution and increase of pest resistance to<br />certain chemical insecticides. One solution can be used to solve these<br />problems is by using resistant variety that is also an integral part of the<br />integrated pest management (IPM). Study on the resistance of several<br />cotton accessions to sucking insect pest, Amrasca biguttula (Ishida) was<br />conducted at the Experimental Station of the Indonesian Tobacco and<br />Fiber Crops Research Institute (IToFCRI) in Asembagus, Situbondo, East<br />Java, from January to December 2006. The objective of the study was to<br />find out the resistance of cotton accessions to sucking insect pest. Thirty<br />accessions of cotton were used as treatment and were planted in plots 10 m<br />x 3 m with plant spacing 100 cm x 25 cm, one plant per hole. Each<br />accession was arranged in a randomized block design with three<br />replications. Parameters observed were number of nymph of A. biguttula,<br />number of leaf hair, leaf hairs position (erect or lie down), and score of<br />damage. The result showed that every accession of cotton can be attacked<br />by A. biguttula although the insect population was not significantly<br />different among accessions. There is negative correlation (R 2 = 0.2425)<br />between number of leaf hair and population of A. biguttula and between<br />number of leaf hair and score of plant damage (R 2 = 0.2027). Accessions<br />that categorized as lightly hairy and moderately resistant to A. biguttula<br />were AC 134, Stoneville 7, Fai Nai, SHR, CRDI-1, Kanesia 5, Kanesia 8,<br />and Kanesia 9, while the others were categorized as glabrous and<br />susceptible to the sucking pest.<br />Key words: Cotton, Gossypium hirsutum, accession, insect pest, Amrasca<br />biguttula (Ishida), tolerant, sensitive, damage, East Jav


2018 ◽  
Vol 30 (1) ◽  
pp. 75 ◽  
Author(s):  
Gul B. Poussio ◽  
Manzoor A. Abro ◽  
Jamal U. D. Hajano ◽  
Muhammad I. Khaskheli ◽  
Khalid I. Rajput ◽  
...  

Fusarium oxysporum f.sp. lycopersici (FOL) is a highly destructive fungal pathogen of tomato crop causing wilt disease which may reduce 10 to 90% yield. In Pakistan, tomato is widely grown in Sindh province, major territories are district Hyderabad, Tando Allahyar and Mirpurkhas. Thus, surveys of these territories were conducted to record intensity of the disease and confirm etiology. Furthermore, potential of different botanical pesticides and commercially available fungicides were tested to inhibit mycelial growth of the causal fungus. The experiment was laid down with complete randomized block design with three replications. The results showed that the disease was occurring in all locations with the range of 8-47 % incidence. F. oxysporum f.sp. lycopersici was predominantly isolated from the collected disease sample during survey and pathogenic nature of the fungus was tested on the tomato Golo variety through soil drenching method. The disease incidence of 30 and 42 % (72 % as total) was recorded in inoculated tomato plants at 20 and 40 DAI, respectively. Maximum (67 %) inhibition of the fungal growth was found by neem seed extracts at higher dose of 8 % concentration followed by 63 % with neem seeds and Eucalyptus at 6 and 8 %, respectively. Alternatively, the Nativo 75 WG fungicide was found most effective in reducing the redial mycelial growth of target fungus followed by Topsin-M at 1000 ppm where as Aliette and Melodedue fungicides were found least effective under in-vitro conditions.


2020 ◽  
Vol 9 (1) ◽  
pp. 73-77
Author(s):  
Hasriadi Mat Akin ◽  
Emi Lidya Astri ◽  
Maimun Barmawi

Segregation pattern of the Soybean Stunt Virus resistant character and genetic diversity of F2:3 families derived from crosses between Orba and B3570.  Soybean stunt disease caused by SSV (Soybean Stunt Virus) is the most destructive soybean disease in Indonesia. This research was conducted from October 2005 to June 2006 at experiment station of Lampung University.  The aims of this research were to evaluate the segregation of resistant characters and total genetic diversity of eight populations of F2:3  families. Experiment was arranged in a randomized complete block design with three replications. The resistance was evaluated based on the score of disease severity.  The results showed that the resistant characters segregate 1:2:1 to susceptible, moderately resistant, and resistant, respectively based on  the segregation pattern. The resistant character was controlled by single gene and the action of the gene is noncompletely dominant gene.  Eight populations of F2:3 families have high diversities on the yield and yield components.


HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 742d-742
Author(s):  
C.J. Coyne ◽  
D.C. Smith ◽  
S.A. Mehlenbacher ◽  
K.B. Johnson ◽  
J.N. Pinkerton

Resistant cultivars are a promising disease control method for eastern filbert blight, which is devastating hazelnut production in Oregon. In 1990, two studies were begun to evaluate the relative resistance of European hazelnut (Coyhls avellana) genotypes to the causal fungus, Anisogramma anomala. A randomized block design of 40 genotypes was planted using inoculated trees planted in the borders as the disease source. The first- and second-year disease incidence (percent) were compared to the published disease incidence (percent) based on exposing potted trees of 44 genotypes to high doses of inoculum. Disease incidence was significantly correlated between the two studies in 1991 (r =0.41, P = 0.02) and in 1992 (r =0.64, P = 0.001; rs = 0.35, 0.025 < P < 0.050). Three genotypes, however, showed no disease in the field, but they had disease in >70% of the potted tree study. A plot of disease incidence in the field planting indicates that the inoculum was present throughout the blocks.


Author(s):  
Ayda Krisnawati ◽  
ANDY SOEGIANTO ◽  
BUDI WALUYO ◽  
KUSWANTO

Abstract. Krisnawati A, Soegianto A, Waluyo B, Kuswanto. 2019. Selection of F6 soybean population for pod shattering resistance. Biodiversitas 20: 3340-3346. Pod shattering is one of the major soybean constraints in Indonesia. This research aimed to evaluate the resistance of soybean F6 population to pod shattering and characterize the selected lines for their agronomic performances. The materials used were 147 F6 lines derived from six crossing combinations. The check varieties consisted of Dega 1, Detap 1, and Anjasmoro. The experiment was arranged in a randomized block design with two replications. At R8 stage, thirty pods were randomly detached from five sample plants of each line to be used for evaluation of pod shattering resistance using an oven-dry method. The variability of pod shattering was showed after the treatment of 60°C oven temperature. The shattering resistance of 147 F6 lines was classified into 52 highly resistant, 49 resistant, two moderately resistant, ten susceptible, and 34 highly susceptible lines. The pod-shattering resistant lines could be used for further improvement in the breeding program. Anjasmoro variety was effective to be used as gene source for shattering resistant. Simultaneous selection based on yield (30% selection intensity) and shattering resistance selected six lines which have early maturity and large seed size, hence they could be further evaluated in several locations in the next breeding stage.


2017 ◽  
Vol 29 (2) ◽  
pp. 257 ◽  
Author(s):  
Muhammad R. Bashir ◽  
Abid Mehmood ◽  
Muhammad Sajid ◽  
Muhammad A. Zeshan ◽  
Muhammad Mohsin ◽  
...  

The current research was conducted to find out the most appropriate concentrations of six fungicides for the management of sesame charcoal rot caused by Macrophomina phaseolina under lab and field conditions. The treatments viz. Antracol, Topsin-M, Mancozeb, Score, Topas, Nativo and Control with concentrations of 150, 250 and 350ppm were used with three replications under completely randomized design and randomized complete block design in Lab. and field conditions respectively. The mean colony growth of all treatments expressed that Nativo exhibited minimum colony growth of (0.93 cm) as compared to Score (1.14 cm), Topsin-M (1.42 cm), Mancozeb (1.77 cm), Antracol (2.04 cm), Topass (2.33 cm) correspondingly. The interaction between treatments and concentrations (T×C) showed that  used concentrations 150 ppm, 250 ppm and 350 ppm of Nativo abundantly inhibit fungal colony growth upto 1.26 cm, 0.86 cm and 0.66 cm respectively whereas the interaction between treatments and days expressed that after day ninth the minimum colony growth (1.23 cm) was observed for Nativo as compared to all other treatments. Similarly, the interaction between concentrations and days expressed highest fungal colony growth at concentration 150 ppm on day third (2.06 cm), sixth (3.02 cm) and ninth (3.65 cm) but the interaction of treatments, days and concentrations expressed that at 150 ppm concentration, all treatments exhibited minimum colony growth (1.70 to 3.30) cm at third, sixth and ninth day as compared to 250 and 350 ppm concentration respectively with respect to control (6.90 cm). In filed conditions, Nativo exhibited minimum Mean Disease Incidence (12.55%) whereas the interaction between treatments and days showed minimum of 14.95%, 12.82% and 9.90% disease incidence by Nativo as compared to all other treatments including control (66.86%, 77.57% and 87.22%) after day tenth, twenty and thirty. It was concluded that Nativo is significantly inhibiting the colony growth under lab and filed conditions.


2014 ◽  
Vol 6 (2) ◽  
pp. 541-544 ◽  
Author(s):  
P. N. Meena ◽  
A. Roy ◽  
B. S. Gotyal ◽  
S. Mitra ◽  
S. Satpathy

Field experiment was conducted for disease management with seven eco-friendly treatments in Corchorus olitorius jute during 2012-2013 under randomized block design (RBD) in three replications with variety JRO-524. The ecofriendly treatment 50% N: P: K + seed treatments with Azotobacter and phosphorus solubilizing bacteria (PSB) @ 5g/Kg+ Trichoderma viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + Psuedomonas fluorescens spray @ 0.2% at 45DAS was found superior with minimum per cent disease incidence (3.46 and 1.43%) as compared to control (13.17 and 4.96%). The second best treatment was found with Farm yard manure (FYM) @ 5t/ha + seed treatment with Azotobecter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha 21DAS)+ P. fluorescencs spray @ 0.2% at 45DAS and showed 3.61 and 2.0% disease incidence. The treatment with N: P: K @ 60:30:30 and seed treatment with carbendazim 50WP @ 2g/Kg at 45DAS showed 4.56 and 2.10 % disease incidence. Dry fibre yield was highest (30.33q/ha) in the plots treated with FYM @ 5t/ha + seed treatment with Azotobacter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + P. fluorescencs spray @ 0.2% at 45DAS) and it was lowest in untreated control (15.69 q/ha).Variation in yield was attributed due to difference in plant height.


2021 ◽  
Vol 10 (12) ◽  
pp. e225101220351
Author(s):  
Andre May ◽  
Luciana Fontes Coelho ◽  
Evandro Henrique Figueiredo Moura da Silva ◽  
Nilson Aparecido Vieira Junior ◽  
Ronaldo da Silva Viana ◽  
...  

The present study proposes to evaluate extracts from shoots of healthy soybean and potato plants for the treatment of plants of the same species cultivated subsequently. Two experiments were thus conducted separately after the soybean and potato inoculum production phases. For soybean, the experiment was laid out in a randomized-block design with five treatments and four replications, in which the following treatments were tested: 1. absolute control without inoculum or pesticides; 2. farmer management with pesticides, without inoculum; 3. use of inoculum, without insecticides; 4. use of inoculum, without fungicides; and 5. use of inoculum, without pesticides. In the soybean crop, the tested inoculum improved plant development and, increased the potassium content of the plant tissue as well as yield. For the potato crop, a randomized-block statistical design was adopted with two treatments and ten replications, using varieties Ágata and Atlantic. The following treatments were tested: 1. absolute control without inoculum, with pesticides; and 2. use of inoculum (foliar spraying), with pesticides. With the use of inoculum, varieties Ágata and Atlantic showed distinct responses regarding disease incidence and yield. Late blight was the disease that most affected both varieties, but only Atlantic showed a reduction in its incidence when the inoculum was applied. Variety Atlantic also exhibited an increase in the most valued commercial calibers, besides an increase in yield, with the use of the tested inoculum. Cultivar Ágata showed a marked reduction in tuber defects with the use of the inoculum on the plants.


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