16S rDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome

ObjectiveTo study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).MethodWe recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).ResultWe found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with Prevotella enterotype when compared to control women with Prevotella enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with Bacteroides enterotype. Ruminococcus gnavus, Prevotella stercorea, Dialister succinatiphilus and Bacteroides fragilis were more abundant while Christensenellaceae spp. were less abundant in the PCOS group. P. stercorea was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.ConclusionOur study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS via drugs and diet.

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Association between Luteinizing Hormone/Choriogonadotropin Receptor Ins18LQ Gene Polymorphism and Polycystic Ovary Syndrome

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.4182 ◽

2020 ◽

Vol 8 (A) ◽

pp. 517-520

Author(s):

Hilma Putri Lubis ◽

Muhammad Fidel Ganis Siregar ◽

Ichwanul Adenin ◽

Binarwan Halim ◽

Henry Salim Siregar ◽

...

Keyword(s):

Luteinizing Hormone ◽

Polycystic Ovary Syndrome ◽

Gene Polymorphism ◽

Polycystic Ovary ◽

Control Group ◽

Control Groups ◽

Ovary Syndrome ◽

Significant Difference ◽

Pcos Group ◽

And Control

BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in the childbearing period. However, its pathophysiology is still unclear. Certain polymorphisms of the luteinizing hormone/choriogonadotropin receptor (LHCGR) genes may lead to changes in the bioactivity of this hormone. The important functional role of LHCGR in the metabolism of androgen and ovulation, the LHCGR gene variant, may be related to the risk of PCOS. AIM: The aim of this study was to evaluate the association between LHCGR Ins18LQ gene polymorphism and PCOS. METHODS: A case–control study was performed in women with PCOS and non-PCOS from May 2019 to October 2019 in HFC IVF Center. We included 50 women with PCOS and 50 healthy controls. Polymorphism of the LHCGR (ins18LQ) gene was genotyped using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: From this study, we found that there was no significant difference in the proportion of ages between the groups (p > 0.05). There were significant differences in the characteristics of body mass index, FSH level, LH level, and LH/FSH ratio between the PCOS and control groups (p < 0.05). We also found that the proportion of heterozygote variant non-ins/ins was higher in the PCOS group compared to the control group, but there was no significant difference between the polymorphisms of the non-ins and non-nonins variants between the PCOS and control groups (p = 0.269). The frequency of ins alleles was higher in the PCOS group compared to the control group. CONCLUSION: There was no significant association between LHCGR ins18LQ gene polymorphism and PCOS.

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Evaluation on the Characteristics of Gut Microbiome in Polycystic Ovary Syndrome Rats Induced by Dihydrotestosterone or Letrozole

10.21203/rs.3.rs-97809/v1 ◽

2020 ◽

Author(s):

Yanhua Zheng ◽

Hongxia Ma ◽

Ying Xu ◽

Chengjie Liang ◽

Tong Yang

Keyword(s):

Polycystic Ovary Syndrome ◽

Gut Microbiota ◽

Aromatase Inhibitor ◽

16S Rdna ◽

High Throughput ◽

Gut Microbiome ◽

High Throughput Sequencing ◽

Polycystic Ovary ◽

Molecular Ecology ◽

Ovary Syndrome

Abstract The etiology of polycystic ovary syndrome (PCOS) is unclear. Recent reports indicated that the gut microbiome of PCOS patients and rodents has changed. In this study, we induced the nonaromatizable androgen dihydrotestosterone (DHT) or the aromatase inhibitor letrozole (LET) to induce PCOS model rat to compare the bacterial diversity distribution within and between the two groups. The molecular ecology of the fecal gut microbiota was analyzed by 16S rDNA high-throughput sequencing. Our study found that DHT can reduce the microbial richness in rats. PCoA plots confirmed that DHT group was statistically significantly separated from C group and LET group. At phylum level, DHT led to a decrease in Bacteroidetes as well as an increase in Cyanobacteria, Tenericutes, Actinobacteria, Spirochaetae and Saccharibacteria. At genus level, LEfSe analysis showed that genus of Bifidobacteriales, Vibro, Peptococcus and Turicibacter played roles in the letrozole induced PCOS rats. And Lachnospiraceae_NK4A136_group, Ruminococcus_1, Ruminiclostridium, Anaerotruncus and Anaeroplasma played vital roles in the intestine of DHT induced PCOS rats.

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Role of Single Nucleotide Variants in FSHR, GNRHR, ESR2 and LHCGR Genes in Adolescents with Polycystic Ovary Syndrome

Diagnostics ◽

10.3390/diagnostics11122327 ◽

2021 ◽

Vol 11 (12) ◽

pp. 2327

Author(s):

Lasma Lidaka ◽

Laine Bekere ◽

Adele Rota ◽

Jekaterina Isakova ◽

Gunta Lazdane ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Total Testosterone ◽

Healthy Controls ◽

Single Nucleotide Variants ◽

Ovary Syndrome ◽

Single Nucleotide ◽

Pcos Group ◽

Risk Patients ◽

Major Allele

Background: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy in women, affecting up to 16.6% of reproductive-age women. PCOS symptoms in adolescents comprise oligomenorrhoea/amenorrhoea and biochemical and/or clinical hyperandrogenism. Long-term health risks of PCOS patients include infertility, metabolic syndrome, type 2 diabetes and cardiovascular disease. Genetic factors have been proven to play a role in development of the syndrome and its symptoms. Objective: To investigate single nucleotide variants (SNVs) in the GNRHR, ESR2, LHCGR and FSHR genes in adolescent patients with PCOS and their association with PCOS symptoms. Methods: We conducted a cross-sectional study comprising of 152 adolescents: 63 patients with PCOS, 22 patients at risk of developing PCOS and 67 healthy controls. Participants were recruited from out-patients attending a gynaecologist at the Children’s Clinical University Hospital, Riga, Latvia, between January 2017 and December 2020. Genomic DNA was extracted from whole blood, and SNVs in the GNRHR, ESR2, LHCGR and FSHR genes were genotyped. The distributions of SNV genotypes were compared among the three groups and genotype-phenotype associations within the PCOS group were evaluated. Results: No statistically significant differences were found in the distributions of genotypes for GNRHR (rs104893837), ESR2 (rs4986938), LHCGR (rs2293275) and FSHR (rs6166, rs6165, rs2349415) among PCOS patients, risk patients and healthy controls. Within the PCOS group, ESR2 rs4986938 minor allele homozygous patients had a significantly higher level of total testosterone than major allele homozygous patients and heterozygous patients. A significantly higher total testosterone level was also observed in PCOS patients carrying the LHCGR rs2293275 minor allele compared with major allele homozygous patients. Conclusions: The SNVs ESR2 rs4986938 and LHCGR rs2293275 play a role in the phenotypic characteristics of PCOS. To fully uncover their influence on the development of PCOS and its symptoms, further studies of larger cohorts and a follow up of this study sample through to adulthood are required. Furthermore, studies of adolescent PCOS patients conducted prior to the latest European Society of Human Reproduction and Embryology (ESHRE) criteria (2018) should be re-evaluated as the study groups might include risk patients according to these updated criteria, thereby potentially significantly impacting the published results.

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Association of SNP rs700519 in CYP19A1 Gene with Polycystic Ovary Syndrome (PCOS) Among Females of Quetta, Pakistan

International Journal of Human Genetics ◽

10.31901/24566330.2021/21.03.782 ◽

2021 ◽

Vol 21 (03) ◽

Author(s):

Rozeena Shaikh

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Cross Sectional Study ◽

Control Group ◽

Ovary Syndrome ◽

Chi Square ◽

Cross Sectional ◽

Significant Difference ◽

Reproductive Disorder ◽

Pcos Group

ABSTRACT Polycystic Ovary Syndrome (PCOS) is endocrine reproductive disorder which causes oligomenorrhea/ amenorrhea, infertility, type II diabetes. The present study aims in CYP19A1 polymorphism rs700519 (C/T) identification that elevates androgen among PCOS females in Quetta, Pakistan. Cross-sectional study involved enrollment of 100 control and 100 affected females. Blood samples were collected for genetic and hormonal analysis. The samples were amplified via ARMS PCR and analyzed by sequencing. The frequency of CC genotype in control and PCOS group was 48 percent and 33 percent. For CT, it was 52 percent and 67 percent. In control group, the allele frequency for C and T was 0.74 and 0.26. In PCOS group, it was 0.67 and 0.33 for C and T, respectively. The Pearson Chi-Square p=0.031 (p<0.05) at 95% Confidence Interval inferred a significant difference between the observed genotypes. The study inferred that CT genotype is a risk factor for PCOS progression in the population of Quetta.

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Association of oxidative stress marker ischemia modified albumin and polycystic ovary syndrome in adolescent and young girls

Turkish Journal of Biochemistry ◽

10.1515/tjb-2018-0088 ◽

2018 ◽

Vol 44 (2) ◽

pp. 161-169

Author(s):

Arzu Kösem ◽

Aytekin Tokmak ◽

Serkan Bodur ◽

Rıfat Taner Aksoy ◽

Canan Topcuoglu ◽

...

Keyword(s):

Oxidative Stress ◽

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Oxidative Stress Marker ◽

Healthy Controls ◽

Ovary Syndrome ◽

Ischemia Modified Albumin ◽

Young Girls ◽

Normal Ranges ◽

Significant Difference

Abstract Objective The pathophysiologic features of polycystic ovary syndrome (PCOS) seem to be a combination of genetic predisposition and environmental factors. However, data regarding the exact effect of oxidative stress on PCOS is conflicting. This cross sectional and case-control study was designed to compare the serum ischemia modified albumin (IMA) levels in adolescent and young girls with and without PCOS. Methods A total of 41 non-obese adolescents and young girls (15–21 years) diagnosed as PCOS and 41 age and body mass index (BMI) matched controls were enrolled to study. The main features of PCOS and markers of chronic inflammation were determined together with serum IMA levels at the time of study enrollment. Results The C-reactive protein and neutrophil-to-lymphocyte ratio were within the normal ranges and also there were no significant difference between the two groups (p>0.05). Serum levels of IMA were significantly increased in adolescents with PCOS respect to healthy controls (0.44±0.12 versus 0.35±0.10 absorbance units, p<0.001). And also there was a significant positive correlation between serum IMA and BMI in all groups (r=0.274, p=0.013). Conclusion Serum IMA levels were higher in PCOS patients than in the healthy controls. This elevation may contribute to the increased cardiovascular diseases risk in PCOS patients.

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The FSHR Polymorphisms Association With Polycystic Ovary Syndrome in Women of Erbil, Kurdistan in North of Iraq

Ibn AL- Haitham Journal For Pure and Applied Science ◽

10.30526/2017.ihsciconf.1799 ◽

2018 ◽

pp. 262 ◽

Cited By ~ 1

Author(s):

Aesha Sh. Sh. Baban ◽

Sabah H. Korsheed ◽

Anas Y. Al Hayawi

Keyword(s):

Polycystic Ovary Syndrome ◽

Clinical Symptoms ◽

Fasting Glucose ◽

Polycystic Ovary ◽

Total Testosterone ◽

Ovary Syndrome ◽

Significant Difference ◽

Pcr Rflp ◽

Serum Hormone ◽

Pcos Group

Polycystic ovary syndrome (PCOS) is an endocrine disorder in women during fertilization age that reflects changing clinical symptoms. The genetic concept of PCOS is unclear and no significant genetic association with PCOS has been established. The level of Follicle stimulating hormone FSH is encoded by FSH receptor (FSHR) and abnormal FSHR affects follicle cogenesis and ovary and consist of 9 introns, 10 exons, and the region of chromosome promoter at 2p21. Sample of 93PCOS patients and 52 controls were collected from Province of Erbil in north of Iraq. Genomic DNA was extracted from the blood and genotype dissected was improved for the two population of study using PCR-RFLP with the restriction enzyme Eam1105I. The genotype distributions and allele frequency of Ala307Thr polymorphisms of FSHR were not statistically various between the controls and the PCOS patients. Significant elevation of body mass index with all genotype of PCOS was found when compared with controls. There were statistical differences in the BMI and most of the serum hormone and lipid profile parameters including LH, total testosterone, fasting glucose, Cholesterol, HDL and LDL, there were significant various in FSH and LH levels of hormones and HDL, LDL and VLDL with PCOS group conveying different genotypes of Ala307Thr polymorphisms. The variant of Ala307Thr was not associated with PCOS in Kurdistan women; there was no relationship between the POCS and gene of FSHR polymorphism at codons 307. There was a significant difference in FSH and LH levels with PCOS patients conveying different genotypes of Ala307Thr polymorphism.

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Serum Asprosin Level in Different Subtypes of Polycystic Ovary Syndrome: A Cross-sectional Study

10.21203/rs.3.rs-41704/v1 ◽

2020 ◽

Author(s):

yonghui jiang ◽

yue liu ◽

zhiheng yu ◽

ping yang ◽

lei xie ◽

...

Keyword(s):

Insulin Resistance ◽

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Laboratory Data ◽

Control Group ◽

Model Assessment ◽

Ovary Syndrome ◽

Cross Sectional ◽

Significant Difference ◽

Pcos Group

Abstract Objective Polycystic ovary syndrome (PCOS) can be divided into different subtypes, including insulin resistance (IR) and hyperandrogenism (HA). Asprosin is a novel hormone associated with IR; however, the role of asprosin in women with PCOS has not been investigated. Thus, the aim of this study was to investigate the relationship between serum asprosin levels and PCOS subtypes. Methods Ninety-three women with PCOS and 77 healthy women as controls were selected for this study. Clinical and laboratory data were compared between the PCOS group and the control group. The PCOS group was further divided into subgroups: 1) women with or without HA (PCOS HA and PCOS NHA, respectively); 2) women with or without IR (PCOS IR and PCOS NIR, respectively). Serum asprosin was measured by ELISA. Results Serum asprosin levels showed no significant difference between the PCOS and control groups. However, it was significantly lower in the PCOS HA and IR groups compared to the respective PCOS NHA and NIR groups (P < .05). In the PCOS group, serum asprosin was negatively correlated with body mass index, luteinizing hormone, testosterone, basal antral follicles, fasting insulin, Homeostatic Model Assessment of Insulin Resistance, and triglycerides. After adjusting for BMI, the correlations were not significant and asprosin was only positively correlated with prolactin (r = 0.426, P < .001). Conclusions Our study shows that women with PCOS HA or IR exhibit significantly lower serum asprosin levels compared to controls, and the lower asprosin level directly correlated with PRL level.

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Anti-Müllerian Hormone as a Diagnostic Marker in Egyptian Infertile Polycystic Ovary Syndrome Females: Correlations with Vitamin D, Total Testosterone, Dyslipidemia and Anthropometric Parameters

Journal of Medical Biochemistry ◽

10.1515/jomb-2017-0068 ◽

2018 ◽

Vol 37 (4) ◽

pp. 448-455 ◽

Cited By ~ 7

Author(s):

Engy Bakeer ◽

Rasha Radwan ◽

Ahmed El Mandoury ◽

Abdullah Abd El Rahman ◽

Mohamed Gad ◽

...

Keyword(s):

Vitamin D ◽

Polycystic Ovary Syndrome ◽

Diagnostic Marker ◽

Polycystic Ovary ◽

Characteristic Curve ◽

Total Testosterone ◽

Healthy Controls ◽

Anthropometric Parameters ◽

Ovary Syndrome ◽

Pcos Group

Summary Background: Recent studies have highlighted the role of anti-Müllerian hormone (AMH) in numerous ovarian disorders. Polycystic Ovary Syndrome (PCOS) is one of the major causes of infertility in Egypt. Several reports have linked PCOS with vitamin D deficiency. This investigation illustrates the possibility of using serum AMH for PCOS diagnosis in infertile Egyptian females, determines the variables affecting it and correlates it with serum 25(OH)D, testosterone, dyslipidemia and anthropometric parameters. Methods: All parameters were assessed either with ELISA or colorimetrically in 53 infertile PCOS women and 17 age matched apparently healthy controls diagnosed according to Rotterdam consensus. Results: Serum AMH, total testosterone, triacylglycerol (TG) levels and BMI were significantly higher in PCOS group compared to healthy controls (p=0.0239, p=0.0381, p=0.0457, and p=0.0067, respectively), while serum 25(OH)D levels and HDL-cholesterol (HDL-C) were significantly lower (p=0.0397 and p=0.0443, respectively). No significant correlation existed between AMH and 25(OH) D, BMI and dyslipidemia markers. AMH was found to have a significant negative correlation with age and a highly significant positive one with total testosterone in PCOS group (r=-0.303, p=0.027 and r=0.370, p=0.008, respectively). In the receiver operating characteristic curve of AMH, the cut-off value was 42.63 pmol/L with a specificity of 59% and a sensitivity of 82%. Multivariate regression analysis showed total testosterone to be the only determinant for AMH (β=0.381 and p=0.038). Conclusions: There should be a future trend of using AMH as a diagnostic marker for PCOS in Egyptian females. The variation in serum AMH levels is determined by total testosterone.

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Association of SNP rs700519 in CYP19A1 Gene with Polycystic Ovary Syndrome (PCOS) Among Females of Quetta, Pakistan

International Journal of Human Genetics ◽

10.31901/24566330.2021/21.02.782 ◽

2021 ◽

Vol 21 (02) ◽

Author(s):

Rozeena Shaikh

Keyword(s):

Polycystic Ovary Syndrome ◽

Polycystic Ovary ◽

Cross Sectional Study ◽

Control Group ◽

Ovary Syndrome ◽

Chi Square ◽

Cross Sectional ◽

Significant Difference ◽

Reproductive Disorder ◽

Pcos Group

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Dysbiosis of the Saliva Microbiome in Patients With Polycystic Ovary Syndrome

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.624504 ◽

2021 ◽

Vol 10 ◽

Author(s):

Na Li ◽

Yangyang Li ◽

Chen Qian ◽

Qing Liu ◽

Wei Cao ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Diurnal Rhythm ◽

Polycystic Ovary ◽

Control Group ◽

Healthy Controls ◽

Ovary Syndrome ◽

Time Points ◽

Diurnal Oscillation ◽

Salivary Microbiota ◽

Pcos Group

Significant differences in salivary microbiota communities between polycystic ovary syndrome (PCOS) patients and healthy controls have been reported, and interestingly, some salivary microbiota exhibit diurnal oscillation in healthy people. However, whether the diurnal oscillation of salivary microbiota is present in PCOS patients is unknown. In this study, we describe the differences in the saliva microbiome between the PCOS group and the control group at different time points over 24 h. 16S rRNA gene amplicon sequencing was performed on salivary and fecal samples from 10 PCOS patients and 10 healthy controls, and salivary samples were collected at 6-h intervals over 24 h (Zeitgeber (ZT)0, ZT6, ZT12, and ZT18). Among the salivary samples, those from the PCOS group showed significant differences from those of the control group at each time point. Differences were evident in taxa level and metabolic pathways. Interestingly, we found that PCOS disrupted the diurnal rhythm of the salivary microbiota abundance, as determined in the group of healthy women. In addition, no similar changes were found in PCOS patients and controls between the oral and fecal microbiota, including differential microbiota at the phylum level. In this study, significant differences in the composition of the salivary microbiota between PCOS and healthy women were detected at different time points. We also showed that the diurnal rhythm of relative abundance of the salivary microbiota was disrupted in patients with PCOS, which might be related to development of oral-related diseases and systematic metabolic disorders.

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