scholarly journals Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

2021 ◽  
Vol 11 ◽  
Author(s):  
Chen Shen ◽  
Qianhui Zhu ◽  
Fan Dong ◽  
Wei Wang ◽  
Bo Fan ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Calcium Oxalate ◽  
White Blood Cells ◽  
Urinary Stones ◽  
High Recurrence Rate ◽  
High Power Field ◽  
Calcium Oxalate Stones ◽  
Rdna Sequencing

Urinary stones and urinary tract infection (UTI) are the most common diseases in urology and they are characterized by high incidence and high recurrence rate in China. Previous studies have shown that urinary stones are closely associated with gut or urine microbiota. Calcium oxalate stones are the most common type of urinary stones. However, the profile of urinary tract microorganisms of calcium oxalate stones with UTI is not clear. In this research, we firstly found two novel clusters in patients with calcium oxalate stones (OA) that were associated with the WBC/HP (white blood cells per high-power field) level in urine. Two clusters in the OA group (OA1 and OA2) were distinguished by the key microbiota Firmicutes and Enterobacteriaceae. We found that Enterobacteriaceae enriched in OA1 cluster was positively correlated with several infection-related pathways and negatively correlated with a few antibiotics-related pathways. Meantime, some probiotics with higher abundance in OA2 cluster such as Bifidobacterium were positively correlated with antibiotics-related pathways, and some common pathogens with higher abundance in OA2 cluster such as Enterococcus were positively correlated with infection-related pathways. Therefore, we speculated that as a sub-type of OA disease, OA1 was caused by Enterobacteriaceae and the lack of probiotics compared with OA2 cluster. Moreover, we also sequenced urine samples of healthy individuals (CK), patients with UTI (I), patients with uric acid stones (UA), and patients with infection stones (IS). We identified the differentially abundant taxa among all groups. We hope the findings will be helpful for clinical treatment and diagnosis of urinary stones.

scholarly journals Prevalence of urinary tract infection and associated risk factors among women in Sindhupalchowk district, Nepal

2018 ◽  
Vol 5 (7) ◽  
pp. 2714 ◽  
Author(s):  
Leela Paudel ◽  
Naresh Manandhar ◽  
Saroj Sah ◽  
Sudesha Khadka ◽  
Samikshya Neupane ◽  
...  
Keyword(s):  
Risk Factors ◽  
Urinary Tract Infection ◽  
Abdominal Pain ◽  
Urinary Tract ◽  
Tract Infection ◽  
Significant Risk ◽  
Lower Abdominal Pain ◽  
Common Symptom ◽  
High Power Field ◽  
Toilet Facility

Background: Urinary tract infection (UTI) occurs in all age groups, more common in women due to short urethra and its close proximity to anus and vagina. UTI is defined as “microscopic finding of >10 pus cells/high power field (40x) in urine”. The purpose of the study is to find the prevalence of UTI and its association with various risk factors.Methods: An analytical cross-sectional study on prevalence of UTI was done among 260 women aged 15 years and above. Convenient sampling technique was used. Semi-structured questionnaire was designed to collect the data and urine sample was collected for routine and microscopic examination at the time of interview. Collected urine was sent, within 3 hours of collection.Results: The mean age of the respondents was 36.43±16.17 years. The prevalence of UTI among women aged 15 years and above was 36.9%.The most common symptom was frequency of micturition (35%) followed by lower abdominal pain (38.46%). There was significant association between frequency of micturition, burning micturition and lower abdominal pain with occurrence of urinary tract infection. On urinalysis, 96 samples were positive for pus cell; one sample showed blood, 16 samples showed ca-oxalate and 57 samples showed protein which determines the type of UTI. Smoking [COR-2.15, C.I-(1.12, 4.09)] and unavailability of toilet facility [COR-0.27, C.I-(0.08, 0.93)] were the significant risk factors for occurrence of UTI.Conclusions: There was high prevalence of UTI among women aged 15 years and above and association between smoking and unavailability of toilet facility and UTI was significant.

scholarly journals Association of pyuria and positive urine culture in children with urinary tract infection

2002 ◽  
Vol 42 (5) ◽  
pp. 197
Author(s):  
Adrian Umboh ◽  
Andy Sugoro
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Abdominal Pain ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Positive Urine Culture ◽  
High Power Field ◽  
Positive Urine ◽  
Upper Abdominal

Background Infections affecting the urinary tract are commonly found in children and responsible as the second cause of morbidity after respiratory tract infections.Objective To detennine the association between pyuria and positive urine culture in children with suspected urinary tract infection (UTI).Methods We reviewed all patients who suffered from suspected UTI with pyuria, aged 1 month to 13 years at the Department of Child Health, Manado Central General Hospital from January 1999 until December 2001.Results Of the 45 patients who suffered from suspected UTI with pyuria, 33 (73%) were proved to have UTI (12 males and 21 females). There was significant association between pyuria of more than 20 white cells per high power field visualized and the incidence of UTI (P <0.05), but no association was found between sex and the incidence of UTI. In patients aged 1 year and older, the rate of UTI was higher in female than male, namely 61 % of females and 30% of males had UTI. The main cause of UTI was Escherichia coli (67%). The most common symptoms were fever (94%), vomiting (76%), and upper abdominal pain (55%).Conclusion There was a significant association between patients whom suspected UTI with pyuria and incidence of UTI.

Role of Dipstick Testing in the Evaluation of Urinary Tract Infection in Nursing Home Residents

2007 ◽  
Vol 28 (7) ◽  
pp. 889-891 ◽  
Author(s):  
Manisha Juthani-Mehta ◽  
Mary Tinetti ◽  
Eleanor Perrelli ◽  
Virginia Towle ◽  
Vincent Quagliarello
Keyword(s):  
Urinary Tract Infection ◽  
Nursing Home ◽  
Urinary Tract ◽  
Tract Infection ◽  
Blood Cells ◽  
Urine Culture ◽  
White Blood Cells ◽  
Nursing Home Residents ◽  
Colony Forming Units

Among 101 nursing home residents with suspected urinary tract infection (UTI), we determined the negative predictive value of dipstick testing for leukocyte esterase and nitrite to be 100% (95% confidence interval, 74%-100%), compared with laboratory evidence of UTI (greater than 10 white blood cells/mm3 on urinalysis and greater than 100,000 colony forming units/mL on urine culture). Nursing home dipstick testing effectively excluded the possibility of UTI.

scholarly journals Urinalysis and Urinary Tract Infection: Update for Clinicians

2001 ◽  
Vol 9 (4) ◽  
pp. 249-255 ◽  
Author(s):  
Jennifer L. Young ◽  
David E. Soper
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Renal Involvement ◽  
Recurrent Infection ◽  
High Power Field ◽  
Symptomatic Urinary Tract Infection ◽  
A Value ◽  
Automated Microscopy

Dysuria is a common presenting complaint of women and urinalysis is a valuable tool in the initial evaluation of this presentation. Clinicians need to be aware that pyuria is the best determinate of bacteriuria requiring therapy and that values significant for infection differ depending on the method of analysis. A hemocytometer yields a value of ≥ 10 WBC/ mm3significant for bacteriuria, while manual microscopy studies show ≥ 8 WBC/high-power field reliably predicts a positive urine culture. In cases of uncomplicated symptomatic urinary tract infection, a positive value for nitrites and leukocyte esterase by urine dipstick can be treated without the need for a urine culture. Automated urinalysis used widely in large volume laboratories provides more sensitive detection of leukocytes and bacteria in the urine.With automated microscopy, a value of > 2 WBC/hpf is significant pyuria indicative of inflammation of the urinary tract. In complicated cases such as pregnancy, recurrent infection or renal involvement, further evaluation is necessary including manual microscopy and urine culture with sensitivities.

scholarly journals Predictors of Acute Pyelonephritis in Infants Less Than 4 Months Old with Febrile Urinary Tract Infection

2020 ◽  
Vol 30 (5) ◽  
Author(s):  
Tung-Yu Lin ◽  
Chih-Chieh Yang ◽  
Nai-Wen Fang ◽  
Jenn-Tzong Chang ◽  
Hsiao-Ping Wang ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Acute Pyelonephritis ◽  
Multiple Logistic Regression Analysis ◽  
White Blood Cells ◽  
Age Group ◽  
Febrile Urinary Tract Infection ◽  
Neutrophil Lymphocyte Ratio ◽  
Young Infants

Background: Detecting early predictors of acute pyelonephritis (APN) is essential for the prognosis, but few studies have focused on young infants specifically. Objectives: The aim of our research was to determine the relationship between APN and laboratory parameters in the age group less than 4 months. Methods: This retrospective study included patients aged less than 4 months with first time febrile urinary tract infection (UTI) between January 2012 and December 2018. White blood cells (WBC), C-reactive protein (CRP), and blood neutrophil/lymphocyte ratio (NLR) were analyzed. Patients were divided into two groups according to the presence of renal defects on dimercaptosuccinic acid (DMSA) scans. Results: In total, 205 patients were screened; 107 patients were in the APN group, and 98 patients were in the non-APN group. Compared with the non-APN group, the APN group showed significant differences in therapeutic response time (TRT), CRP, and NLR (all P values < 0.001). Multiple logistic regression analysis revealed that CRP, NLR, and TRT were independent risk factors for APN (P ≤ 0.001, 0.003, and 0.004, respectively). The area under the receiver operating characteristic (ROC) curve was 0.774 for CRP (P < 0.001). The optimum cut-off value for CRP was 4.27 mg/dL, with the highest sensitivity and specificity (70.1% and 73.5%, respectively). Conclusions: In the age group less than 4 months, without the image diagnosis, we could treat the patients as APN for longer antibiotic duration if CRP ≥ 4.27 mg/dL.

Sign in / Sign up

Export Citation Format

Share Document