scholarly journals Glutathione Fine-Tunes the Innate Immune Response toward Antiviral Pathways in a Macrophage Cell Line Independently of Its Antioxidant Properties

2017 ◽  
Vol 8 ◽  
Author(s):  
Marina Diotallevi ◽  
Paola Checconi ◽  
Anna Teresa Palamara ◽  
Ignacio Celestino ◽  
Lucia Coppo ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Line ◽  
Innate Immune Response ◽  
Antioxidant Properties ◽  
Innate Immune ◽  
Macrophage Cell Line ◽  
Macrophage Cell

scholarly journals 2,2’4,4’-Tetrabromodiphenyl Ether (PBDE-47) Modulates the Intracellular miRNA Profile, sEV Biogenesis and Their miRNA Cargo Exacerbating the LPS-Induced Pro-Inflammatory Response in THP-1 Macrophages

2021 ◽  
Vol 12 ◽  
Author(s):  
Valeria Longo ◽  
Alessandra Longo ◽  
Giorgia Adamo ◽  
Antonino Fiannaca ◽  
Sabrina Picciotto ◽  
...  
Keyword(s):  
Immune Response ◽  
Inflammatory Response ◽  
Innate Immune Response ◽  
De Novo ◽  
In Silico Analysis ◽  
Innate Immune ◽  
Macrophage Cell ◽  
Intracellular Expression ◽  
Different Levels ◽  
Silico Analysis

The 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) is one of the most prominent PBDE congeners detected in the environment and in animal and human tissues. Animal model experiments suggested the occurrence of PBDE-induced immunotoxicity leading to different outcomes and recently we demonstrated that this substance can impair macrophage and basophil activities. In this manuscript, we decided to further examine the effects induced by PBDE-47 treatment on innate immune response by looking at the intracellular expression profile of miRNAs as well as the biogenesis, cargo content and activity of human M(LPS) macrophage cell-derived small extracellular vesicles (sEVs). Microarray and in silico analysis demonstrated that PBDE-47 can induce some epigenetic effects in M(LPS) THP-1 cells modulating the expression of a set of intracellular miRNAs involved in biological pathways regulating the expression of estrogen-mediated signaling and immune responses with particular reference to M1/M2 differentiation. In addition to the cell-intrinsic modulation of intracellular miRNAs, we demonstrated that PBDE-47 could also interfere with the biogenesis of sEVs increasing their number and selecting a de novo population of sEVs. Moreover, PBDE-47 induced the overload of specific immune related miRNAs in PBDE-47 derived sEVs. Finally, culture experiments with naïve M(LPS) macrophages demonstrated that purified PBDE-47 derived sEVs can modulate macrophage immune response exacerbating the LPS-induced pro-inflammatory response inducing the overexpression of the IL-6 and the MMP9 genes. Data from this study demonstrated that PBDE-47 can perturb the innate immune response at different levels modulating the intracellular expression of miRNAs but also interfering with the biogenesis, cargo content and functional activity of M(LPS) macrophage cell-derived sEVs.

scholarly journals Neisseria gonorrhoeaeInduces a Tolerogenic Phenotype in Macrophages to Modulate Host Immunity

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Alejandro Escobar ◽  
Enzo Candia ◽  
Sebastian Reyes-Cerpa ◽  
Bélgica Villegas-Valdes ◽  
Tanya Neira ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Line ◽  
Mhc Class Ii ◽  
Transmitted Disease ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Sexually Transmitted ◽  
Antigen Presenting

Neisseria gonorrhoeaeis the etiological agent of gonorrhoea, which is a sexually transmitted disease widespread throughout the world.N. gonorrhoeaedoes not improve immune response in patients with reinfection, suggesting that gonococcus displays several mechanisms to evade immune response and survive in the host.N. gonorrhoeaeis able to suppress the protective immune response at different levels, such as B and T lymphocytes and dendritic cells. In this study, we determined whetherN. gonorrhoeaedirectly conditions the phenotype of RAW 264.7 murine macrophage cell line and its response. We established that gonococcus was effectively phagocytosed by the RAW 264.7 cells and upregulates production of immunoregulatory cytokines (IL-10 and TGF-β1) but not the production of proinflammatory cytokine TNF-α, indicating that gonococcus induces a shift towards anti-inflammatory cytokine production. Moreover,N. gonorrhoeaedid not induce significant upregulation of costimulatory CD86 and MHC class II molecules. We also showed thatN. gonorrhoeaeinfected macrophage cell line fails to elicit proliferative CD4+ response. This implies that macrophage that can phagocytose gonococcus do not display proper antigen-presenting functions. These results indicate thatN. gonorrhoeaeinduces a tolerogenic phenotype in antigen-presenting cells, which seems to be one of the mechanisms to induce evasion of immune response.

Cytotoxicity and immune response of CdSe/ZnS Quantum dots towards a murine macrophage cell line

RSC Advances ◽  
2014 ◽  
Vol 4 (11) ◽  
pp. 5792 ◽  
Author(s):  
Guimiao Lin ◽  
Zhangchi Ding ◽  
Rui Hu ◽  
Xiaomei Wang ◽  
Qiang Chen ◽  
...  
Keyword(s):  
Immune Response ◽  
Quantum Dots ◽  
Cell Line ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

Fasciola hepatica — the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke

2013 ◽  
Vol 58 (4) ◽  
Author(s):  
Piotr Bąska ◽  
Anna Zawistowska-Deniziak ◽  
Anna Zdziarska ◽  
Katarzyna Wasyl ◽  
Marcin Wiśniewski ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Line ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Effective Vaccine ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Recombinant Antigens ◽  
Tnf Α

AbstractFasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.

scholarly journals Piscirickettsia salmonis Imbalances the Innate Immune Response to Succeed in a Productive Infection in a Salmonid Cell Line Model

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0163943 ◽  
Author(s):  
Claudio A. Álvarez ◽  
Fernando A. Gomez ◽  
Luis Mercado ◽  
Ramón Ramírez ◽  
Sergio H. Marshall
Keyword(s):  
Immune Response ◽  
Cell Line ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Productive Infection ◽  
Line Model ◽  
Cell Line Model ◽  
Piscirickettsia Salmonis

scholarly journals The Vibrio cholerae Cytotoxin (VCC) Simultaneously Activates Responses of Death, Inflammation (NF-κB) and Survival (AP-1) through the MAPK Signaling in Human Macrophages THP-1

2019 ◽  
Author(s):  
Julio R. Escartín-Gutiérrez ◽  
Aldo Arturo Reséndiz Albor ◽  
Carlos Alberto Castañón-Sánchez ◽  
Rafael Campos-Rodríguez ◽  
Gustavo Pedraza-Alva ◽  
...  
Keyword(s):  
Immune Response ◽  
Vibrio Cholerae ◽  
Innate Immune Response ◽  
Mapk Signaling ◽  
Innate Immune ◽  
Human Macrophage ◽  
Activated Macrophages ◽  
Macrophage Cell ◽  
Nf Kappa B

The human innate immune response to the pore-forming toxin of Vibrio cholerae VCC, is currently under study. Here, in vitro studies on a human macrophage cell line (THP-1), helped explore the activated pathways involved on the onset the innate immune response towards the cytotoxin. The secreted monomeric 65 KDa form interacts with mature macrophages in pg/ml concentrations, determined by dose response experiments after treatments under 1 h. Non vacuolating concentrations (pg/ml) were applied to the cells; immunoblots revealed activation of MAPKs: early overexpression of p38 and ERK. Cell lysis by release of lactate dehydrogenase (LDH) was not apparent in the first hour, nonetheless it was positive after 24 h. Finally, to discern whether the VCC stimulates transcriptional activators via MAPKs pathway, NF-κB and AP-1 were studied by real time quantitation. Increased expression of p50 (NF-κB), cJun and cFos (AP-1) was observed. Given that NF-κB is the transcription factor initiating inflammation of innate immune response and in turn, AP-1 is responsible for cell surviving response, results from this study lead us to conclude that VCC in vitro treatments, induce a pro-inflammatory and a surviving response, in less than one hour on activated macrophages.

Sign in / Sign up

Export Citation Format

Share Document