Synergistic Protective Effect of Konjac Mannan Oligosaccharides and Bacillus subtilis on Intestinal Epithelial Barrier Dysfunction in Caco-2 Cell Model and Mice Model of Lipopolysaccharide Stimulation

As the first line of defense against intestinal bacteria and toxins, intestinal epithelial cells are always exposed to bacteria or lipopolysaccharide (LPS), whereas pathogenic bacteria or LPS can cause intestinal epithelial cell damage. Previous studies have shown that konjac mannan oligosaccharides (KMOS) have a positive effect on maintaining intestinal integrity, and Bacillus subtilis (BS) can promote the barrier effect of the intestine. However, it is still unknown whether KMOS and BS have a synergistic protective effect on the intestines. In this study, we used the LPS-induced Caco-2 cell injury model and mouse intestinal injury model to study the synergistic effects of KMOS and BS. Compared with KMOS or BS alone, co-treatment with KMOS and BS significantly enhanced the activity and antioxidant capacity of Caco-2 cell, protected mouse liver and ileum from LPS-induced oxidative damage, and repaired tight junction and mucus barrier damage by up-regulating the expression of Claudin-1, ZO-1 and MUC-2. Our results demonstrate that the combination of KMOS and BS has a synergistic repair effect on inflammatory and oxidative damage of Caco-2 cells and aIIeviates LPS-induced acute intestinal injury in mice.

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Transcytosis of Bacillus subtilis extracellular vesicles through an in vitro intestinal epithelial cell model

Scientific Reports ◽

10.1038/s41598-020-60077-4 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Ana Paula Domínguez Rubio ◽

Jimena Martínez ◽

Marcos Palavecino ◽

Federico Fuentes ◽

Christian Miquel Sánchez López ◽

...

Keyword(s):

Bacillus Subtilis ◽

Epithelial Cell ◽

Extracellular Vesicles ◽

Intestinal Epithelial Cell ◽

Cell Model ◽

Intestinal Epithelial

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Protective effect of chitooligosaccharides on hydrogen peroxide-induced PC-12 cells death by inhibition of oxidative damage

Cell Biology International ◽

10.1042/cbi034s027d ◽

2010 ◽

Vol 34 (8) ◽

pp. S27-S27

Author(s):

Xueling Dai ◽

Ping Chang ◽

Ke Xu ◽

Changjun Lin ◽

Hanchang Huang ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Oxidative Damage ◽

Protective Effect ◽

Pc 12 Cells ◽

Cells Death

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Protective effect of Korean ginseng berry on hydrogen peroxide (H2O2)-induced oxidative damage in SH-SY5Y cells

10.1055/s-0037-1608447 ◽

2017 ◽

Author(s):

A Jin Seul ◽

J Byun ◽

Y Ban Ju

Keyword(s):

Hydrogen Peroxide ◽

Oxidative Damage ◽

Protective Effect ◽

Korean Ginseng

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Search for Compounds Suppressing Intestinal α-Glucosidase Expression in Caco-2 Cells

ICONIET PROCEEDING ◽

10.33555/iconiet.v2i2.18 ◽

2019 ◽

Vol 2 (2) ◽

pp. 96-101

Author(s):

Kota Noda ◽

Eisuke Kato ◽

Jun Kawabata

Keyword(s):

Blood Glucose ◽

Intestinal Tract ◽

Cell Model ◽

Epigallocatechin Gallate ◽

Calluna Vulgaris ◽

Active Principle ◽

Intestinal Epithelial ◽

Mrna Expression Level ◽

Epicatechin Gallate ◽

Control Post

Diabetes is a chronic disease characterized by elevated blood glucose level.Reducing carbohydrate absorption from the intestinal tract is an effective strategy to control post-meal blood glucose level. Inhibition of intestinal α-glucosidase, involved in digestion of carbohydrates, is known as an approach to accomplish this. On the other hand, reduction of α-glucosidase amount is expected to work in the similar manner. However, none of the previousstudy pursues this approach. A convenient assay was developed to evaluate α-glucosidase amount employing Caco-2 cells, the intestinal epithelial cell model reported to express α-glucosidase. Sixty plants were screened and two candidate plants, Calluna vulgaris and Perilla frutescens var. crispa were found to reduce α-glucosidase expression. C. vulgaris extract was subjected to activity guided isolation. Proanthocyanidin was identified as the active principle which was analyzed by thiol decomposition to reveal the components as a mixture ofcatechin, epicatechin, epigallocatechin, and A type procyanidin dimer. The proanthocyanidin suppressed about 30% of α-glucosidase amount evaluated through convenient assay, and suppressed bulk of mRNA expression level of sucrase-isomaltase (SI) at 0.125 mg/mL. Several flavan-3-ol monomers were also tested, and epicatechin gallate and epigallocatechin gallate were found to suppress α-glucosidase amount significantly.

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ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.28930/jitkt.v1i2.7871 ◽

2009 ◽

Vol 1 (2) ◽

Author(s):

Risa Nofiani ◽

Siti Nurbetty ◽

Ajuk Sapar

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Pathogenic Bacteria ◽

Methanol Extract ◽

Agar Medium ◽

Antimicrobial Activities ◽

Antimicrobial Compounds ◽

Associated Bacteria ◽

Minimum Inhibitory Concentrations ◽

Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

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Faculty Opinions recommendation of Clinical trial: protective effect of a commercial fish protein hydrolysate against indomethacin (NSAID)-induced small intestinal injury.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.722677375.578882 ◽

2008 ◽

Author(s):

Ingvar Bjarnason

Keyword(s):

Clinical Trial ◽

Protective Effect ◽

Protein Hydrolysate ◽

Intestinal Injury ◽

Fish Protein ◽

Commercial Fish ◽

Small Intestinal Injury ◽

Fish Protein Hydrolysate ◽

Small Intestinal

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Protective Effect of Chungkukjang from Sunchang Province against Cellular Oxidative Damage

Preventive Nutrition and Food Science ◽

10.3746/jfn.2008.13.2.090 ◽

2008 ◽

Vol 13 (2) ◽

pp. 90-94 ◽

Cited By ~ 2

Author(s):

Ji-Myung Choi ◽

Na-Ri Yi ◽

Kyoung-Chun Seo ◽

Ji-Sook Han ◽

Young-Ok Song ◽

...

Keyword(s):

Oxidative Damage ◽

Protective Effect

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Alcohol Induced Hepato Cardiotoxicity and Oxidative Damage in Rats: The Protective Effect of n-butanol Extract of Green Tea (Camellia sinensis (L.) Kuntze)

Cardiovascular & Haematological Disorders - Drug Targets ◽

10.2174/1871529x17666170310101727 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 2

Author(s):

Amel Amrani ◽

Nassima Boubekri ◽

Somia Lassad ◽

Djamila Zama ◽

Fadil Benayache ◽

...

Keyword(s):

Oxidative Damage ◽

Protective Effect ◽

Green Tea ◽

Camellia Sinensis ◽

Butanol Extract

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SIRT1 attenuates sepsis-induced acute kidney injury via Beclin1 deacetylation-mediated autophagy activation

Cell Death and Disease ◽

10.1038/s41419-021-03508-y ◽

2021 ◽

Vol 12 (2) ◽

Author(s):

Zhiya Deng ◽

Maomao Sun ◽

Jie Wu ◽

Haihong Fang ◽

Shumin Cai ◽

...

Keyword(s):

Acute Kidney Injury ◽

Protective Effect ◽

Cell Model ◽

Kidney Injury ◽

Underlying Mechanism ◽

Autophagy Induction ◽

Promising Therapeutic Approach ◽

Related Proteins ◽

Caecal Ligation And Puncture

AbstractOur previous studies showed that silent mating-type information regulation 2 homologue-1 (SIRT1, a deacetylase) upregulation could attenuate sepsis-induced acute kidney injury (SAKI). Upregulated SIRT1 can deacetylate certain autophagy-related proteins (Beclin1, Atg5, Atg7 and LC3) in vitro. However, it remains unclear whether the beneficial effect of SIRT1 is related to autophagy induction and the underlying mechanism of this effect is also unknown. In the present study, caecal ligation and puncture (CLP)-induced mice, and an LPS-challenged HK-2 cell line were established to mimic a SAKI animal model and a SAKI cell model, respectively. Our results demonstrated that SIRT1 activation promoted autophagy and attenuated SAKI. SIRT1 deacetylated only Beclin1 but not the other autophagy-related proteins in SAKI. SIRT1-induced autophagy and its protective effect against SAKI were mediated by the deacetylation of Beclin1 at K430 and K437. Moreover, two SIRT1 activators, resveratrol and polydatin, attenuated SAKI in CLP-induced septic mice. Our study was the first to demonstrate the important role of SIRT1-induced Beclin1 deacetylation in autophagy and its protective effect against SAKI. These findings suggest that pharmacologic induction of autophagy via SIRT1-mediated Beclin1 deacetylation may be a promising therapeutic approach for future SAKI treatment.

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