Bacillus subtilis BSH has a protective effect on Salmonella infection by regulating the intestinal flora structure in chickens

2021 ◽  
pp. 104898
Author(s):  
Jun-Hong Xing ◽  
Qiong-Yan Li ◽  
Wei Zhao ◽  
Gui-Lian Yang ◽  
Rong-Rong Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Protective Effect ◽  
Intestinal Flora ◽  
Salmonella Infection

scholarly journals Estimation of permeability to bacteriophages of intestinal mucosa of chickens with eimeriosis

2019 ◽  
Vol 49 (2) ◽  
pp. 57-63
Author(s):  
N. V. Davydova ◽  
V. Yu. Koptev ◽  
Yu. N. Kozlova ◽  
L. I. Sulimova ◽  
V. N. Afonyushkin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Protective Effect ◽  
Intestinal Mucosa ◽  
Clinical Picture ◽  
Biological Material ◽  
Probiotic Strain ◽  
Field Isolates ◽  
Different Types ◽  
Inflammatory Processes

In the course of the study permeability of intestinal mucosa of chickens suffering from eimeriosis while treating them with various veterinary drugs, including probiotics, was evaluated. The simulation of a typical clinical picture of eimeriosis was carried out by oral administration of suspension with coccidial oocysts (1.6 × 105/head) using a probe. To create different forms and different intensity of inflammatory processes, chickens that received various anticoccidial preparations and probiotic strain Bacillus subtilis were infected with eimeria. According to the data from an autopsy, it was found that the use of a spore probiotic based on Bacillus subtilis and anticoccidial drugs containing robenidine hydrochloride and salinomycin had a positive protective effect when treating chickens from eimeriosis. A similar picture was observed when assessing permeability of intestinal mucosa as affected by bacteriophage, whereby permeability decreased with the use of probiotics and the above-mentioned active agents. In general, the decrease in productivity was significant in all groups. However, the effect of spore-based probiotics was quite pronounced against the background of eimeria polyresistance. In the situation where anticoccidial drugs are less effective, the use of a spore-based probiotic can have a noticeable protective effect. The effect of all anticoccidial drugs under study on the concentration of oocysts and the state of the mucosa was insignificant, which indicated polyresistance of different types of eimeria isolated from biological material to these drugs. The analysis of the intestinal mucosa integrity, based on the study of mucosa permeability to bacteriophages and a Johnson and Reid scoring procedure showed that a spore probiotic based on B. subtilis and anticoccidial drugs containing robenidine and salinomycin had the best protective effect against eimeriosis caused by field isolates of eimeria. When treating chickens suffering from eimeriosis caused by polyresistant forms of E. acervulina and E. tenella, it is advisable to use probiotics alongside with drugs based on robenidine and salinomycin.

scholarly journals Effects of administration of Bacillus subtilis strain BN on intestinal flora of weanling piglets.

1981 ◽  
Vol 43 (5) ◽  
pp. 771-775 ◽  
Author(s):  
Kyosuke OZAWA ◽  
Hiroshi YOKOTA ◽  
Makoto KIMURA ◽  
Tomotari MITSUOKA
Keyword(s):  
Bacillus Subtilis ◽  
Intestinal Flora ◽  
Subtilis Strain ◽  
Weanling Piglets

Prevention of Salmonella Infection in Chicks by Treatment with Fecal Cultures from Mature Chickens (Nurmi Cultures)

1981 ◽  
Vol 44 (12) ◽  
pp. 909-916 ◽  
Author(s):  
H. PIVNICK ◽  
B. BLANCHFIELD ◽  
J. -Y. D'AOUST
Keyword(s):  
Drinking Water ◽  
Protective Effect ◽  
Salmonella Typhimurium ◽  
Intestinal Tract ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Gallus Domesticus ◽  
Salmonella Infection

Chicks (Gallus domesticus) were treated per os with 24-h-old anaerobic cultures of feces from mature chickens 1 day after hatching, challenged with Salmonella typhimurium in the drinking water 2 days later, and sacrificed on day 11 or 12; then the lower third of the intestinal tract was examined for salmonellae. Cultures of feces inoculated directly into the crop or added to the drinking water, even after holding at −70 C for 21 days, protected chicks against infection by S. typhimurium. Cultures serially subcultured daily up to four times were protective, and dilution to 1:80 in drinking water containing 4 % skim milk powder did not decrease their protective effect. Treated chicks were about 1000-fold more resistant to infection by Salmonella than untreated chicks.

scholarly journals Effects of Mixed Probiotic Spores of Bacillus Coagulans and Bacillus Subtilis on Gut Microbiota and Intestinal and Liver Damage of Rats Infected with Salmonella Typhimurium

2020 ◽  
Author(s):  
Somaye Mazkour ◽  
Seyed Shahram Shekarforoush ◽  
Sara Basiri ◽  
Fatemeh Namazi ◽  
Forough Zarei-Kordshouli
Keyword(s):  
Bacillus Subtilis ◽  
Mononuclear Cells ◽  
Bacillus Coagulans ◽  
Coliform Bacteria ◽  
Intestinal Lumen ◽  
Control Group ◽  
Salmonella Infection ◽  
Early Recovery ◽  
Daily Water Intake ◽  
Histopathological Effects

Abstract Background: Although the intestinal lumen is a major way for the entrance of pathogens such as Salmonella, they face a dense microbiota in the gut lumen. This community confers health benefits. The probiotics enhance the colonization resistance and direct inhibitory effects against pathogens, which are important in reducing the incidence, duration of gastroenteritis and also histopathological effects. Methods: In this study spore of two probiotic bacteria, Bacillus subtilis and Bacillus coagulans were fed to rats for three weeks through their daily water intake after which Salmonella Typhimurium was gavaged to the rats. On days 1, 3, 5 and 7 after gavaging, intestinal microbiota and days 1, 3, 5 and 7 after gavaging, gut and liver histopathological changes were evaluated. Results: In comparison to the control group, Salmonella caused no change in spore and aerobic bacteria population but it caused anaerobic and coliform bacteria increase and LAB reduction. But B. subtilis and B. coagulans accompaniment with salmonella reduced the anaerobe and coliform bacteria and decreased the LAB population following Salmonella infection. Also, the histopathological evaluation showed that these probiotic attenuated shortening and fusion of villi, mononuclear cells infiltration, submucosal edema and epithelial necrosis in the intestine, hepatocyte degeneration and mononuclear cells infiltration in the liver which occurred due to Salmonella infection.Conclusion: B. subtilis and B. coagulans help the maintenance of the gut microflora population and reduce the histopathological effects when exposing to the Salmonella, which, causes the reduction of this pathogen effects and early recovery.

scholarly journals Application of endophytic bacteria Bacillus subtilis in compositions with salicylic acid to improve wheat stress tolerance

2020 ◽  
Author(s):  
D. Garshina ◽  
A. Ibragimov ◽  
O. Lastochkina
Keyword(s):  
Bacillus Subtilis ◽  
Salicylic Acid ◽  
Drought Stress ◽  
Protective Effect ◽  
Stress Tolerance ◽  
Endophytic Bacteria ◽  
Maximum Growth ◽  
Stress Conditions

Maximum growth-stimulating and protective effect of endophytic bacteria Bacillus subtilis 10-4 on wheat plants under normal and drought stress conditions reached when its applied in composition with salicylic acid were discovered.

Antagonistic activity and protective effect of a Bacillus subtilis isolate against fish pathogen Edwardsiella piscicida

2019 ◽  
Vol 85 (6) ◽  
pp. 1011-1018
Author(s):  
Xiaomeng Ren ◽  
Binbin Wu ◽  
Feng Zhao ◽  
Lingling Qi ◽  
Xianfeng Qiu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Protective Effect ◽  
Antagonistic Activity ◽  
Fish Pathogen ◽  
Edwardsiella Piscicida

scholarly journals Prevention and Curation of DSS-induced IBD in Mice With Bacillus Subtilis Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora

2020 ◽  
Author(s):  
Xuan Zhang ◽  
Yanjun Tong ◽  
Xiaomei Lyu ◽  
Jin Wang ◽  
Yuxue Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Inflammatory Response ◽  
Oral Administration ◽  
Intestinal Mucosa ◽  
Inflammatory Responses ◽  
Activity Index ◽  
Intestinal Flora ◽  
Fermented Milk ◽  
Mucosal Barrier ◽  
Therapeutic Effects

Abstract Background: The pathogenesis of inflammatory bowel disease (IBD) might be related to the local inflammatory damage and the dysbacteriosis of intestinal flora. Probiotics can regulate the intestinal flora and ameliorate IBD. The probiotic Bacillus subtilis strain B. subtilis JNFE0126 was used as the starter of fermented milk. However, the therapeutic effects of B. subtilis fermented milk on IBD remains to be explored.Methods：The therapeutic effect of the B. subtilis fermented milk on DSS-induced IBD model mice was evaluated. The disease activity index (DAI) and the pathological features of small intestinal and colonic mucosa were examined. For exploring the action mechanism of B. subtilis, immunohistochemical staining and western-blotting were used to analyse the expression of the pro-inflammatory/anti-inflammatory cytokines, the proliferation of the intestinal stem cells, and the reconstruction of the mucosa barrier. The alteration of gut microbiota was investigated by taxonomic analysis.Results: The DAI of IBD was significantly decreased through oral administration of B. subtilis (JNFE0126) fermented milk, and the intestinal mucosa injury was attenuated. Moreover, B. subtilis could reduce the inflammatory response of the intestinal mucosa, induce proliferation of the intestinal stem cell, and promote reconstruction of the mucosal barrier. Furthermore, B. subtilis could rebalance the intestinal flora, increasing the abundance of Bacillus, Alistipes and Lactobacillus, while decreasing the abundance of Escherichia and Bacteroides.Conclusion: Oral administration of the B. subtilis fermented milk could alleviate DSS-induced IBD via inhibition of inflammatory response, promotion of the mucosal barrier reconstruction and regulation of the intestinal flora.

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