scholarly journals Immunization With Bovine Herpesvirus-4-Based Vector Delivering PPRV-H Protein Protects Sheep From PPRV Challenge

2021 ◽  
Vol 12 ◽  
Author(s):  
Daniel Rodríguez-Martín ◽  
José Manuel Rojas ◽  
Francesca Macchi ◽  
Valentina Franceschi ◽  
Luca Russo ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Control Strategies ◽  
Bovine Herpesvirus ◽  
Diagnostic Tools ◽  
New Approach ◽  
Virus Shedding ◽  
Bovine Herpesvirus 4 ◽  
Ifn Γ ◽  
Herpesvirus 4 ◽  
H Protein

The Morbillivirus peste des petits ruminants virus (PPRV) is the causal agent of a highly contagious disease that mostly affects sheep and goats and produces considerable losses in developing countries. Current PPRV control strategies rely on live-attenuated vaccines, which are not ideal, as they cannot differentiate infected from vaccinated animals (DIVA). Recombinant vector-based vaccines expressing viral subunits can provide an alternative to conventional vaccines, as they can be easily paired with DIVA diagnostic tools. In the present work, we used the bovine herpesvirus-4-based vector (BoHV-4-A) to deliver PPRV hemagglutinin H antigen (BoHV-4-A-PPRV-H-ΔTK). Vaccination with BoHV-4-A-PPRV-H-ΔTK protected sheep from virulent PPRV challenge and prevented virus shedding. Protection correlated with anti-PPRV IgGs, neutralizing antibodies and IFN-γ-producing cells induced by the vaccine. Detection of antibodies exclusively against H-PPRV in animal sera and not against other PPRV viral proteins such as F or N could serve as a DIVA diagnostic test when using BoHV-4-A-PPRV-H-ΔTK as vaccine. Our data indicate that BoHV-4-A-PPRV-H-ΔTK could be a promising new approach for PPRV eradication programs.

scholarly journals Recombinant Bovine Herpesvirus 4 (BoHV-4) Expressing Glycoprotein D of BoHV-1 Is Immunogenic and Elicits Serum-Neutralizing Antibodies against BoHV-1 in a Rabbit Model

2006 ◽  
Vol 13 (11) ◽  
pp. 1246-1254 ◽  
Author(s):  
Gaetano Donofrio ◽  
Sandro Cavirani ◽  
Alain Vanderplasschen ◽  
Laurent Gillet ◽  
Cesidio Filippo Flammini
Keyword(s):  
Neutralizing Antibodies ◽  
Animal Species ◽  
Hepatitis Virus ◽  
Genetic Material ◽  
Rabbit Model ◽  
Bovine Herpesvirus ◽  
Woodchuck Hepatitis Virus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

ABSTRACT Several biological characteristics of bovine herpesvirus 4 (BoHV-4) make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types coming from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. Starting from BoHV-4 cloned as a bacterial artificial chromosome (BAC), we used MuA transposase-mediated in vitro transposition to generate recombinant BoHV-4 expressing the immunodominant glycoprotein D (gD) of BoHV-1, one of the most important pathogens of cattle. Although a cis-acting element from woodchuck hepatitis virus (the woodchuck hepatitis virus posttranscriptional regulatory element [WPRE]) in the 3′ end of the gD expression cassette was required for maximal gD expression from plasmids in transient transfection assays, this element was not necessary for efficient expression of gD from recombinant BoHV-4 genomes. BoHV-4 recombinants containing gD expression cassettes with or without the WPRE expressed gD at similarly high levels. Several cell lines originating from different animal species expressed gD when infected with BoHV-4 recombinants. When rabbits were immunized with one of the recombinants, high levels of serum neutralizing antibodies against BoHV-1 were generated. This work is one of the first demonstrations of the use BoHV-4 as a vector for vaccine purposes and may provide the basis for BoHV-1 vaccination of cattle with recombinant BoHV-4.

scholarly journals Cellular Targeting of Engineered Heterologous Antigens Is a Determinant Factor for Bovine Herpesvirus 4-Based Vaccine Vector Development

2009 ◽  
Vol 16 (11) ◽  
pp. 1675-1686 ◽  
Author(s):  
Gaetano Donofrio ◽  
Valentina Franceschi ◽  
Antonio Capocefalo ◽  
Simone Taddei ◽  
Chiara Sartori ◽  
...  
Keyword(s):  
Recombinant Virus ◽  
Neutralizing Antibodies ◽  
Vaccine Development ◽  
Bovine Herpesvirus ◽  
Artificial Chromosome ◽  
Diarrhea Virus ◽  
Chimeric Peptide ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4 ◽  
Protein Cell

ABSTRACT In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development.

scholarly journals Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function

Reproduction ◽  
2007 ◽  
Vol 134 (1) ◽  
pp. 183-197 ◽  
Author(s):  
Gaetano Donofrio ◽  
Shan Herath ◽  
Chiara Sartori ◽  
Sandro Cavirani ◽  
Cesidio Filippo Flammini ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Bovine Herpesvirus ◽  
Endocrine Function ◽  
Uterine Disease ◽  
Endometrial Stromal Cells ◽  
Persistently Infected ◽  
Endometrial Cells ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

Bovinepostpartumuterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the γ-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses in endometrial pathology and the mechanisms of viral infection of uterine cells are often ignored. The objectives of the present study were to explore the interaction, tropism and outcomes of BoHV-4 challenge of endometrial stromal and epithelial cells. Endometrial stromal and epithelial cells were purified and infected with a recombinant BoHV-4 carrying an enhanced green fluorescent protein (EGFP) expression cassette to monitor the establishment of infection. BoHV-4 efficiently infected both stromal and epithelial cells, causing a strong non-apoptotic cytopathic effect, associated with robust viral replication. The crucial step for the BoHV-4 endometriotropism appeared to be after viral entry as there was enhanced transactivation of the BoHV-4 immediate early 2 gene promoter following transient transfection into the endometrial cells. Infection with BoHV-4 increased cyclooxygenase 2 protein expression and prostaglandin estradiol secretion in endometrial stromal cells, but not epithelial cells. Bovine macrophages are persistently infected with BoHV-4, and co-culture with endometrial stromal cells reactivated BoHV-4 replication in the persistently infected macrophages, suggesting a symbiotic relationship between the cells and virus. In conclusion, the present study provides evidence of cellular and molecular mechanisms, supporting the concept that BoHV-4 is a pathogen associated with uterine disease.

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

scholarly journals Dot Immunobinding Assay for Detection of Bovine Herpesvirus 4 Antibodies in Rabbits

1999 ◽  
Vol 11 (3) ◽  
pp. 237-239 ◽  
Author(s):  
Mohamed Essmail ◽  
Dale Baker ◽  
James Collins ◽  
Sue Vandewoude ◽  
Mohamed Salman ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132212 ◽  
Author(s):  
Erika González Altamiranda ◽  
Julieta M. Manrique ◽  
Sandra E. Pérez ◽  
Glenda L. Ríos ◽  
Anselmo C. Odeón ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
Bovine Embryos ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4

2015 ◽  
Vol 58 (5) ◽  
pp. 725-731 ◽  
Author(s):  
Ernesto Renato Krüger ◽  
Tânia Regina Penha ◽  
Fernanda Cristina Hummelgen ◽  
Jorge Bacila Agottani ◽  
Demétrio Reva ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Bovine Herpesvirus ◽  
Serological Survey ◽  
Specific Antibodies ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Bovine herpesvirus 4 DNA is not detected in free-ranging domestic cats from California, Colorado or Florida

2016 ◽  
Vol 19 (2) ◽  
pp. 235-239 ◽  
Author(s):  
Elliott Chiu ◽  
Ryan M Troyer ◽  
Michael R Lappin ◽  
Sue VandeWoude
Keyword(s):  
Urinary Tract ◽  
Natural Infection ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Domestic Cat ◽  
Domestic Cats ◽  
High Exposure ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4 ◽  
Free Ranging

Objectives Several studies have reported that domestic cats can be naturally infected with bovine herpesvirus 4 (BHV4). Cats experimentally inoculated with BHV4 developed clinical signs involving the urinary tract, leading to the hypothesis that natural infection with BHV4 may be associated with feline lower urinary tract diseases. However, the question of whether BHV4 infection is common in cats remains equivocal. In this study, we sought to determine whether BHV4 is a common natural infection of domestic cats in the USA. Methods We used a sensitive nested PCR protocol specific to the BHV4 thymidine kinase gene to screen free-ranging domestic cat blood DNA samples (n = 101) collected from California, Colorado and Florida. Results Cats within this cohort were positive for seven other common pathogens of domestic cats, demonstrating the relatively high exposure of this population to endemic feline infections. In contrast, all domestic cat blood samples were negative for BHV4, while BHV4-containing tissue culture extracts were strongly positive. Conclusions and relevance BHV4 has been detected in tissues of latently infected cattle, though viral DNA is typically also detected in peripheral blood cells throughout infection. Our results suggest that persistent presence of BHV4 DNA in the blood of domestic cats is either rare or non-existent. We thus conclude that BHV4 is unlikely to be a major pathogen of cats.

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