Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions

Host-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of acute infection where channel catfish were infected with Yersinia ruckeri. The infected fish showed signs of body surface hyperemia as well as hyperemia and swelling in the trunk kidney. Double RNA sequencing was performed on trunk kidneys extracted from infected channel catfish and transcriptome data was compared with data from uninfected trunk kidneys. Results revealed that the host-pathogen interaction was dynamically regulated and that the host-pathogen transcriptome fluctuated during infection. More specifically, these data revealed that the expression levels of immune genes involved in Cytokine-cytokine receptor interactions, the NF-kappa B signaling pathway, the JAK-STAT signaling pathway, Toll-like receptor signaling and other immune-related pathways were significantly upregulated. Y. ruckeri mainly promote pathogenesis through the flagellum gene fliC in channel catfish. The weighted gene co-expression network analysis (WGCNA) R package was used to reveal that the infection of catfish is closely related to metabolic pathways. This study contributes to the understanding of the host-pathogen interaction between channel catfish and Y. ruckeri, more specifically how catfish respond to infection through a transcriptional perspective and how this infection leads to enteric red mouth disease (ERM) in these fish.

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The small RNA ErsA plays a role in the regulatory network of Pseudomonas aeruginosa pathogenicity in airways infection

10.1101/2020.06.22.164558 ◽

2020 ◽

Author(s):

Silvia Ferrara ◽

Alice Rossi ◽

Serena Ranucci ◽

Ida De Fino ◽

Alessandra Bragonzi ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Inflammatory Response ◽

Small Rna ◽

Acute Infection ◽

Host Pathogen Interaction ◽

Envelope Stress ◽

Host Pathogen ◽

Lung Infections ◽

Host Inflammatory Response ◽

Stimulation Of

AbstractBacterial small RNAs play a remarkable role in the regulation of functions involved in host-pathogen interaction. ErsA is a small RNA of Pseudomonas aeruginosa that contributes to the regulation of bacterial virulence traits such as biofilm formation and motility. Shown to take part in a regulatory circuit under the control of the envelope stress response sigma factor σ22, ErsA targets post-transcriptionally the key virulence-associated gene algC. Moreover, ErsA contributes to biofilm development and motility through the post-transcriptional modulation of the transcription factor AmrZ. Intending to evaluate the regulatory relevance of ErsA in the pathogenesis of respiratory infections, we analyzed the impact of ErsA-mediated regulation on the virulence potential of P. aeruginosa and the stimulation of the inflammatory response during the infection of bronchial epithelial cells and a murine model. Furthermore, we assessed ErsA expression in a collection of P. aeruginosa clinical pulmonary isolates and investigated the link of ErsA with acquired antibiotic resistance by generating an ersA gene deletion mutant in a multidrug-resistant P. aeruginosa strain which has long been adapted in the airways of a cystic fibrosis (CF) patient. Our results show that the ErsA-mediated regulation is relevant for the P. aeruginosa pathogenicity during acute infection and contributes to the stimulation of the host inflammatory response. Besides, ErsA could be subjected to selective pressure for P. aeruginosa patho-adaptation and acquirement of resistance to antibiotics commonly used in clinical practice during chronic CF infections. Our findings establish the role of ErsA as an important regulatory element in the host-pathogen interaction.Author summaryPseudomonas aeruginosa is one of the most critical multi-drug resistant opportunistic pathogen in humans, able to cause both lethal acute and chronic lung infections. Thorough knowledge of the regulatory mechanisms involved in the establishment and persistence of the airways infections by P. aeruginosa remains elusive. Emerging candidates as molecular regulators of pathogenesis in P. aeruginosa are small RNAs, which act post-transcriptionally as signal transducers of host cues. Known for being involved in the regulation of biofilm formation and responsive to envelope stress response, we show that the small RNA ErsA can play regulatory roles in acute infection, stimulation of host inflammatory response, mechanisms of acquirement of antibiotic resistance and adaptation during the chronic lung infections of cystic fibrosis patients. Elucidating the complexity of the networks regulating host-pathogen interaction is crucial to identify novel targets for future therapeutic applications.

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Faculty Opinions recommendation of A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726419717.793534336 ◽

2017 ◽

Author(s):

Burkhard Tümmler

Keyword(s):

Outer Membrane ◽

Membrane Vesicles ◽

Outer Membrane Vesicles ◽

Host Pathogen Interaction ◽

Host Pathogen ◽

Bacterial Outer Membrane

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Identification of key mRNAs and lncRNAs involved in the effects of anti-TWEAK on Osteosarcoma

Current Bioinformatics ◽

10.2174/1574893615999200626191405 ◽

2020 ◽

Vol 15 ◽

Author(s):

Mingxuan Yang ◽

Liangtao Zhao ◽

Xuchang Hu ◽

Haijun Feng ◽

Xuewen Kang

Keyword(s):

Dna Replication ◽

Signaling Pathway ◽

Bioinformatics Analysis ◽

Mapk Signaling ◽

Migration And Invasion ◽

Type I ◽

Interferon Signaling ◽

Nf Kappa B ◽

Ppi Networks ◽

Coexpression Networks

Background: Osteosarcoma (OS) is one of the most common primary malignant bone tumors in teenagers. Emerging studies demonstrated TWEAK and Fn14 were involved in regulating cancer cell differentiation, proliferation, apoptosis, migration and invasion. Objective: The present study identified differently expressed mRNAs and lncRNAs after anti-TWEAK treatment in OS cells using GSE41828. Methods: We identified 922 up-regulated mRNAs, 863 downregulated mRNAs, 29 up-regulated lncRNAs, and 58 down-regulated lncRNAs after anti-TWEAK treatment in OS cells. By constructing PPI networks, we identified several key proteins involved in anti-TWEAK treatment in OS cells, including MYC, IL6, CD44, ITGAM, STAT1, CCL5, FN1, PTEN, SPP1, TOP2A, and NCAM1. By constructing lncRNAs coexpression networks, we identified several key lncRNAs, including LINC00623, LINC00944, PSMB8-AS1, LOC101929787. Result: Bioinformatics analysis revealed DEGs after anti-TWEAK treatment in OS were involved in regulating type I interferon signaling pathway, immune response related pathways, telomere organization, chromatin silencing at rDNA, and DNA replication. Bioinformatics analysis revealed differently expressed lncRNAs after antiTWEAK treatment in OS were related to telomere organization, protein heterotetramerization, DNA replication, response to hypoxia, TNF signaling pathway, PI3K-Akt signaling pathway, Focal adhesion, Apoptosis, NF-kappa B signaling pathway, MAPK signaling pathway, FoxO signaling pathway. Conclusion: : This study provided useful information for understanding the mechanisms of TWEAK underlying OS progression and identifying novel therapeutic markers for OS.

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Genomic analysis and functional characterization of immune genes from the RIG-I- and MAVS-mediated antiviral signaling pathway in lamprey

Genomics ◽

10.1016/j.ygeno.2021.04.030 ◽

2021 ◽

Author(s):

Anqi Ma ◽

Meng Gou ◽

Tao Song ◽

Jun Li ◽

Yigao Zhu ◽

...

Keyword(s):

Signaling Pathway ◽

Functional Characterization ◽

Genomic Analysis ◽

Antiviral Signaling ◽

Immune Genes

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The Role of Macrophages in the Host’s Defense against Sporothrix schenckii

Pathogens ◽

10.3390/pathogens10070905 ◽

2021 ◽

Vol 10 (7) ◽

pp. 905

Author(s):

Estela Ruiz-Baca ◽

Armando Pérez-Torres ◽

Yolanda Romo-Lozano ◽

Daniel Cervantes-García ◽

Carlos A. Alba-Fierro ◽

...

Keyword(s):

Immune Responses ◽

Macrophage Polarization ◽

Sporothrix Schenckii ◽

Macrophage Cell ◽

Cell Recruitment ◽

Host Pathogen Interaction ◽

Interaction Processes ◽

Host Pathogen ◽

Molecular Patterns

The role of immune cells associated with sporotrichosis caused by Sporothrix schenckii is not yet fully clarified. Macrophages through pattern recognition receptors (PRRs) can recognize pathogen-associated molecular patterns (PAMPs) of Sporothrix, engulf it, activate respiratory burst, and secrete pro-inflammatory or anti-inflammatory biological mediators to control infection. It is important to consider that the characteristics associated with S. schenckii and/or the host may influence macrophage polarization (M1/M2), cell recruitment, and the type of immune response (1, 2, and 17). Currently, with the use of new monocyte-macrophage cell lines, it is possible to evaluate different host–pathogen interaction processes, which allows for the proposal of new mechanisms in human sporotrichosis. Therefore, in order to contribute to the understanding of these host–pathogen interactions, the aim of this review is to summarize and discuss the immune responses induced by macrophage-S. schenckii interactions, as well as the PRRs and PAMPs involved during the recognition of S. schenckii that favor the immune evasion by the fungus.

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