scholarly journals Thymoquinone Inhibits Virulence Related Traits of Cronobacter sakazakii ATCC 29544 and Has Anti-biofilm Formation Potential

2017 ◽  
Vol 8 ◽  
Author(s):  
Chao Shi ◽  
Chunhong Yan ◽  
Yue Sui ◽  
Yi Sun ◽  
Du Guo ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Cronobacter Sakazakii ◽  
Formation Potential

Investigate of the Ability of Cronobacter sakazakii Isolated from Clinical Samples of Children Under Two Years to Induce Swimming, Swarming and Biofilm

2018 ◽  
pp. 123
Author(s):  
Luma Abdal Hady Zwein ◽  
Tharieyt Abdulrahman Motlag ◽  
Mohamed Mousa
Keyword(s):  
Cerebrospinal Fluid ◽  
Congo Red ◽  
Biofilm Formation ◽  
Microtiter Plate ◽  
Clinical Samples ◽  
Cronobacter Sakazakii ◽  
Yellow Color ◽  
Golden Yellow ◽  
Vitek 2 ◽  
Biochemical Examination

      The study included 200 samples were collected   from   children  under two   years included (50 samples from each of Cerebrospinal fluid, Blood, Stool and Urine) from, Central Children Hospital and Children's Protections Educational Hospital. Isolates bacterial were obtained cultural, microscopic and biochemical examination and diagnosed to the species by using vitek2 system. The results showed there were contamination in 6.5% of clinical samples. The diagnosed colonies which gave pink color on the MacConkey agar , golden yellow color on the Trypton Soy agar and green color on the Birillent Enterobacter sakazakii agar and gave  a probability of 99% in the vitek 2 and were identified as Cronobacter sakazakii. The identification revealed of thirteen isolates: 6(46.16%) isolated from Cerebrospinal fluid samples, 7(53.84%) isolated from blood samples and not isolated bacteria from stool and urine samples. The results of the investigation of some virulence factors showed that all bacteria isolates were able to swimming with a diameter ranging (1-9 mm) and swarming with a diameter ranging (1-40 mm) and their  ability to biofilm formation  by using three methods. The results show the ability  of  isolates to form biofilm by using  Congo red media  methods where it is 12 (92.30 %) out of 13 isolated bacteria belonging to C. sakazakii  able to form biofilm on the Congo red media  which is 3 (23.07%) were  strong production  biofilm ,   8 (61.53%)  were intermediate  production  biofilm and  1 (7.69% ) were weak  biofilm formation , while the 1 (7.69%)  unable to form biofilm.  Tubes method were all isolates were able to form biofilm, it were found that 3 (23.07%)  isolates strong, and 8 (61.53%) intermediate  and 2( 15.38%)  weak biofilm formation. Microtiter plate method  gave 5 (38.46 %) isolates strong, 6 (46.15%) intermediate and 1 (7.69%) weak biofilm formation.  

scholarly journals Roles of outer membrane protein W (OmpW) on survival, morphology, and biofilm formation under NaCl stresses in Cronobacter sakazakii

2018 ◽  
Vol 101 (5) ◽  
pp. 3844-3850 ◽  
Author(s):  
Yingwang Ye ◽  
Na Ling ◽  
Jina Gao ◽  
Xiyan Zhang ◽  
Maofeng Zhang ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Biofilm Formation ◽  
Cronobacter Sakazakii

scholarly journals Importance of Enterococcus spp. for Forming a Biofilm

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S354-S356 ◽  
Author(s):  
L. Necidová ◽  
B. Janštová ◽  
S. Karpíšková ◽  
Š. Cupáková ◽  
M. Dušková ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Dairy Products ◽  
Formation Potential ◽  
Farm Level ◽  
Milk Samples ◽  
Bulk Milk ◽  
Dairy Plant ◽  
Plant Level

The aim of this study was to monitore the capability of <I>Enterococcus fecalis</I> and <I>Enterococcus fecium</I> to form biofilms. Enterococci isolates originated from individual milk, bulk milk samples and environmental swabs obtained at farm level, dairy plant level including semi and final dairy products. Biofilm formation potential was determined by growing the tested strains in glas tubes containing BHI medium. The capability of forming biofilms was detected in 28% of <I>Enterococcus</I> spp. strains. Higher number of biofilm forming strains of the <I>Enterococcus faecium</I> (33%) than <I>Enterococcus faecalis</I> (28%) has been registered. Isolates obtained at plant level were forming biofilms more often than isolates from plant level and in final products (cheese and curd cheese), no isolate has been seen to be able to form biofilm.

scholarly journals Biofilm formation potential and chlorine resistance of typical bacteria isolated from drinking water distribution systems

RSC Advances ◽  
2020 ◽  
Vol 10 (52) ◽  
pp. 31295-31304 ◽  
Author(s):  
Zebing Zhu ◽  
Lili Shan ◽  
Fengping Hu ◽  
Zehua Li ◽  
Dan Zhong ◽  
...  
Keyword(s):  
Drinking Water ◽  
Microbial Communities ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Formation Potential ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution ◽  
Safety Of Drinking Water

Biofilms are the main carrier of microbial communities throughout drinking water distribution systems (DWDSs), and strongly affect the safety of drinking water.

Effects of Ca2+ and Mg2+ on the Biofilm Formation of Cronobacter Sakazakii Strains from Powdered Infant Formula

2015 ◽  
Vol 35 (3) ◽  
pp. 416-421 ◽  
Author(s):  
Yingwang Ye ◽  
Na Ling ◽  
Rui Jiao ◽  
Qingping Wu ◽  
Yongjia Han ◽  
...  
Keyword(s):  
Infant Formula ◽  
Biofilm Formation ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii

scholarly journals Genes Involved in Cronobacter sakazakii Biofilm Formation

2010 ◽  
Vol 76 (7) ◽  
pp. 2251-2261 ◽  
Author(s):  
Isabel Hartmann ◽  
Paula Carranza ◽  
Angelika Lehner ◽  
Roger Stephan ◽  
Leo Eberl ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Strong Impact ◽  
Powdered Infant Formula ◽  
Intestinal Epithelial ◽  
Hypothetical Proteins ◽  
Cronobacter Sakazakii ◽  
Cellular Processes ◽  
Abiotic Surfaces ◽  
Transposon Mutants ◽  
Microtiter Assay

ABSTRACT Cronobacter spp. are opportunistic food-borne pathogens that can cause severe and sometimes lethal infections in neonates. In some outbreaks, the sources of infection were traced to contaminated powdered infant formula (PIF) or contaminated utensils used for PIF reconstitution. In this study, we investigated biofilm formation in Cronobacter sakazakii strain ES5. To investigate the genetic basis of biofilm formation in Cronobacter on abiotic surfaces, we screened a library of random transposon mutants of strain ES5 for reduced biofilm formation using a polystyrene microtiter assay. Genetic characterization of the mutants led to identification of genes that are associated with cellulose biosynthesis and flagellar structure and biosynthesis and genes involved in basic cellular processes and virulence, as well as several genes whose functions are currently unknown. In two of the mutants, hypothetical proteins ESA_00281 and ESA_00282 had a strong impact on flow cell biofilm architecture, and their contribution to biofilm formation was confirmed by genetic complementation. In addition, adhesion of selected biofilm formation mutants to Caco-2 intestinal epithelial cells was investigated. Our findings suggest that flagella and hypothetical proteins ESA_00281 and ESA_00282, but not cellulose, contribute to adhesion of Cronobacter to this biotic surface.

scholarly journals Comparative analysis of carbapenemases, RND family efflux pumps and biofilm formation potential among Acinetobacter baumannii strains with different carbapenem susceptibility

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yanpeng Zhang ◽  
Bing Fan ◽  
Yong Luo ◽  
Zhiyuan Tao ◽  
Yongbo Nie ◽  
...  
Keyword(s):  
Cell Division ◽  
Comparative Analysis ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Carbapenem Resistance ◽  
Efflux Pumps ◽  
Relative Expression ◽  
Formation Potential ◽  
Tertiary Hospitals

Abstract Aim This study has conducted a comparative analysis of common carbapenemases harboring, the expression of resistance-nodulation-cell division (RND) family efflux pumps, and biofilm formation potential associated with carbapenem resistance among Acinetobacter baumannii (A. baumannii) strains with different carbapenem susceptibility. Methods: A total of 90 isolates of A. baumannii from two tertiary hospitals of China were identified and grouped as carbapenem susceptible A. baumannii (CSAB) strains and carbapenem non-susceptible A. baumannii (CnSAB) strains based on the susceptibility to imipenem. Harboring of carbapenemase genes, relative expression of RND family efflux pumps and biofilm formation potential were compared between the two groups. Result: Among these strains, 12 (13.3 %) strains were divided into the CSAB group, and 78 (86.7 %) strains into the CnSAB group. Compared with CSAB strains, CnSAB strains increased distribution of blaOXA−23 (p < 0.001) and ISAba1/blaOXA−51−like (p = 0.034) carbapenemase genes, and a 6.1-fold relative expression of adeB (p = 0.002), while CSAB strains led to biofilm formation by 1.3-fold than CnSAB strains (p = 0.021). Conclusions Clinically, harboring more blaOXA−23−like and ISAba1/blaOXA−51−like complex genes and overproduction of adeABC are relevant with carbapenem resistance, while carbapenem susceptible strains might survive the stress of antibiotic through their ability of higher biofilm formation.

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